This document outlines a proposed study on chronic inflammatory diseases between several partner organizations. The study would examine over 1,000 patients with conditions like chronic gastritis, rheumatoid arthritis, and irritable bowel disease using clinical exams, imaging, molecular genetics analysis, and other methods. It aims to identify common genetic and organ-specific factors involved, understand immune response regulation mechanisms, and estimate microRNA expression and roles to inform new diagnostics and immunomodulation therapies.

1. Partners:
- The Donetsk national medical university
Department of Histology, Cytology and Embryology
Department of Internal Medicine
Department of Medical Biophysics, Medical Equipment and Clinical Informatics
- Rheumatologic Clinic
- Institute of Urgent and Recovery Surgery
Laboratory of Fundamental Research

2. CHRONIC INFLAMMATORY DISEASES
Aggressive factors :
microbes (H. pylori), acid,
bile, enzymes, nicotine,
alcohol, drugs
INJURY
Persistent inflammation
Immunological reactivity
Tissues reactivity (GF, BAS, local regulators)
INTEGRATING MECHANISMS (Neuro-Endocrine reactivity)
GENETIC FACTORS

3. CHRONIC INFLAMMATORY DISEASES
Unclear points:
-What genes and organospecific factors are involved?
- What are the common mechanisms of association
between inflammatory diseases of joints and digestive
system?
- What cells and molecules should be the targets for
therapeutic action of pharmaceutical agents?

4. NEURO
TRANSMITTERS
Proposition
LOCAL IMMUNE STATE
REGULATION BLOOD CELLS
CHRONIC INFLAMMATION Changes of stem cells kinetics
TISSUES DESTRUCTION Violation of reparation
NUTRIENTS. TOXINS,
DRUGS

5. PROPOSED STUDY DESIGN
PATIENTS WITH:
- CHRONIC GASTRITIES; PEPTIC ULCERS (~200 patients),
- CHRONIC PANCREATITIS (~200 patients), IRRITABLE BOWEL DISEASE (~200 patients)
- RHEUMATOID ARTHRITIS, SYSTEMIC LUPUS ERYTHEMATOSUS, POLYMYOSITIS,
SYSTEMIC SCLEROSIS, ANKYLOSING SPONDYLITIS (~400 patients)
METHODS:
I . CLINICAL
I I . X-RAYS
I I I . DENSITOMETRY
I V. ATOMIC SPECTROMETRY
V. IMMUNOENZYME ANALYSIS
MOLECULAR GENETIC ANALYSIS

6. Approach for analysis
Investigation of
Platelets INDUCTION BY: INTERACTIONS
-aggregation -Neurotransmitters BETWEEN BLOOD
-Hormones CELLS
-Cytokines
Leukocytes:
- NADPH-oxidase INHIBITION WITH
- L-arginine -receptor blockers
metabolism
- Cytokine
- Inhibitors of
production enzymes and
-Phagocytosis transductors
Investigation of the EXPRESSION PATTERN AND
FUNCTION OF miRNAs

7. EXPEXTED RESULTS
1. Key factors of reactivity which are associated with chronic
inflammatory diseases
2. Informative criteria for early diagnostics and prognosis of
chronic inflammatory diseases
3. Understanding of mechanisms of immune response regulation
- for development of ways of immunomodulation.
4. Estimation of expression and role of miRNA in the MSCs will
certify or revoke their therapeutic use for the control of
autoimmunity.

8. THANK YOU FOR ATTANTION