Cluster analysis identified 4 clusters of childhood asthma in a study of 161 children with severe asthma. Cluster 1 had relatively normal lung function and less atopy. Cluster 2 had slightly lower lung function, more atopy, and increased symptoms and medication use. Cluster 3 had greater comorbidity, increased bronchial responsiveness, and lower lung function. Cluster 4 had the lowest lung function and greatest symptoms and medication use. The clusters did not correspond to current definitions of asthma severity. Larger longitudinal studies are needed to validate the clusters.
The document discusses HIV/AIDS among people over 50 years old. It references a CDC report from 2005 that shows the number of people over 50 living with HIV has been increasing, both due to improved treatment allowing people to live longer, and new infections in that age group. The proposed methodology would survey doctors and nurses to disseminate prevention and intervention messages to patients at private clinics and personal doctors' offices.
Sinusitis and Immunodeficiency - IDF Conferencesinusblog
This is Dr. Andrew Pugliese's powerpoint on the connection between chronic sinusitis and immunodeficiencies. This was specifically for an educational conference for the Immune Deficiency Foundation.
A study published in early 2019 examined the connection between thyroid function and the onset of atrial fibrillation (AF) by analyzing over 37,000 patient medical records. The researchers found that genetic variations resulting in even slightly higher or lower thyroid hormone levels within normal ranges can impact the risk of developing AF. The findings suggest doctors should consider treating subclinical hyperthyroidism with drugs, as that may reduce AF risk.
Vitamin D and Multiple Sclerosis - An Annotated BiographyKat Venegas
This document provides an annotated bibliography summarizing several studies on the relationship between vitamin D and multiple sclerosis (MS). The studies found:
1) Early supplementation trials in the 1980s saw a decrease in MS relapse rates with calcium, magnesium and vitamin D. However, the sample size was small.
2) Childhood sun exposure reduced MS risk in monozygotic twins, supporting other findings that sun exposure before age 15 prevents MS.
3) A longitudinal study found similar low vitamin D levels in MS patients and controls in Finland. Relapses occurred when vitamin D levels were low and parathyroid hormone levels were high.
4) A cohort study found women with higher vitamin D intake had
1. Acute rheumatic fever (ARF) is an autoimmune response to strep throat infection that can lead to long-term heart damage known as rheumatic heart disease (RHD).
2. While penicillin remains the primary treatment, ARF and RHD continue to be a major problem in low-income countries.
3. Recent research has provided a better understanding of the true global burden of RHD and identified priorities like vaccine development, early detection of RHD, and improving quality of life for those affected.
The document discusses the future of allergy and clinical immunology. It notes that medicine is moving from a reactive "one size fits all" approach to a more personalized approach based on disease stratification, biomarkers, and targeted treatments. This personalized approach will rely on large datasets, reclassification of diseases according to causal pathways, and a systems approach integrating multiple levels of data from patients.
This document summarizes a study investigating the role of autophagy in lung inflammation and corticosteroid-resistant neutrophilic asthma. The study found that deletion of the autophagy gene Atg5 specifically in CD11c+ cells caused spontaneous airway hyperreactivity and severe neutrophilic lung inflammation in mice. Mice lacking Atg5 had higher IL-17A and IL-1β levels in the lungs and increased IL-17A-producing T cells, indicating autophagy impairment induces neutrophilic inflammation through IL-17A secretion. The study also found that lack of autophagy in immune cells, but not lung epithelial cells, contributed to lung inflammation. This suggests
The document discusses HIV/AIDS among people over 50 years old. It references a CDC report from 2005 that shows the number of people over 50 living with HIV has been increasing, both due to improved treatment allowing people to live longer, and new infections in that age group. The proposed methodology would survey doctors and nurses to disseminate prevention and intervention messages to patients at private clinics and personal doctors' offices.
Sinusitis and Immunodeficiency - IDF Conferencesinusblog
This is Dr. Andrew Pugliese's powerpoint on the connection between chronic sinusitis and immunodeficiencies. This was specifically for an educational conference for the Immune Deficiency Foundation.
A study published in early 2019 examined the connection between thyroid function and the onset of atrial fibrillation (AF) by analyzing over 37,000 patient medical records. The researchers found that genetic variations resulting in even slightly higher or lower thyroid hormone levels within normal ranges can impact the risk of developing AF. The findings suggest doctors should consider treating subclinical hyperthyroidism with drugs, as that may reduce AF risk.
Vitamin D and Multiple Sclerosis - An Annotated BiographyKat Venegas
This document provides an annotated bibliography summarizing several studies on the relationship between vitamin D and multiple sclerosis (MS). The studies found:
1) Early supplementation trials in the 1980s saw a decrease in MS relapse rates with calcium, magnesium and vitamin D. However, the sample size was small.
2) Childhood sun exposure reduced MS risk in monozygotic twins, supporting other findings that sun exposure before age 15 prevents MS.
3) A longitudinal study found similar low vitamin D levels in MS patients and controls in Finland. Relapses occurred when vitamin D levels were low and parathyroid hormone levels were high.
4) A cohort study found women with higher vitamin D intake had
1. Acute rheumatic fever (ARF) is an autoimmune response to strep throat infection that can lead to long-term heart damage known as rheumatic heart disease (RHD).
2. While penicillin remains the primary treatment, ARF and RHD continue to be a major problem in low-income countries.
3. Recent research has provided a better understanding of the true global burden of RHD and identified priorities like vaccine development, early detection of RHD, and improving quality of life for those affected.
The document discusses the future of allergy and clinical immunology. It notes that medicine is moving from a reactive "one size fits all" approach to a more personalized approach based on disease stratification, biomarkers, and targeted treatments. This personalized approach will rely on large datasets, reclassification of diseases according to causal pathways, and a systems approach integrating multiple levels of data from patients.
This document summarizes a study investigating the role of autophagy in lung inflammation and corticosteroid-resistant neutrophilic asthma. The study found that deletion of the autophagy gene Atg5 specifically in CD11c+ cells caused spontaneous airway hyperreactivity and severe neutrophilic lung inflammation in mice. Mice lacking Atg5 had higher IL-17A and IL-1β levels in the lungs and increased IL-17A-producing T cells, indicating autophagy impairment induces neutrophilic inflammation through IL-17A secretion. The study also found that lack of autophagy in immune cells, but not lung epithelial cells, contributed to lung inflammation. This suggests
Chikungunya as a Cause of Acute Febrile Illness in Southern Sri LankaYan'an Hou
This study investigated chikungunya virus (CHIKV) as a cause of acute febrile illness in southern Sri Lanka during a 2007 outbreak. Researchers enrolled 797 patients presenting with undifferentiated fever at a large hospital. Serology identified acute CHIKV infection in 3.5% of patients without acute dengue virus infection, and PCR/viral isolation confirmed 64.3% of these cases. Sequencing of CHIKV isolates showed they possessed the E1-226A residue and were closely related to contemporary Sri Lankan and Indian isolates. Except for more frequent and persistent musculoskeletal symptoms, acute CHIKV mimicked dengue and other febrile illnesses clinically. Only 1.
Frequency of migraine headaches in patients with fibromyalgiaPaul Coelho, MD
- The study evaluated the frequency of migraine headaches in a large cohort of patients with fibromyalgia using a brief migraine screening tool.
- Of the 1730 patients with fibromyalgia who completed the survey, 966 (55.8%) met the criteria for migraine headaches based on the screening tool.
- Several comorbid conditions such as depression, anxiety, chronic fatigue syndrome, and irritable bowel syndrome were found to be significantly more common in those fibromyalgia patients who also met the criteria for migraines.
The number of existing functional somatic syndromes (fs ss) is an important r...Paul Coelho, MD
The study tested the hypothesis that the number of existing functional somatic syndromes (FSSs; e.g. fibromyalgia, chronic fatigue syndrome, irritable bowel syndrome) predicts the development of new FSSs. The study found that the incidence of a new FSS increased with the number of pre-existing (antecedent) FSSs in both cases (women with interstitial cystitis) and controls. Specifically, the risk of a new FSS was highest for individuals with 3 or more antecedent FSSs. Logistic regression showed that the number of antecedent FSSs significantly predicted new FSSs even after accounting for other risk factors. This supports the idea that FSSs are linked
A Prospective Observational Study of Zinc As Adjunct Therapy In Pediatric Pop...iosrjce
IOSR Journal of Dental and Medical Sciences is one of the speciality Journal in Dental Science and Medical Science published by International Organization of Scientific Research (IOSR). The Journal publishes papers of the highest scientific merit and widest possible scope work in all areas related to medical and dental science. The Journal welcome review articles, leading medical and clinical research articles, technical notes, case reports and others.
The document discusses cardiovascular disease in HIV patients. It notes that HIV can predispose patients to cardiovascular diseases, and that antiretroviral drugs used to treat HIV can impact lipid profiles. Elderly HIV patients are also more susceptible to comorbidities like cardiovascular disease. The document outlines the need for primary, secondary, and tertiary prevention strategies like education, individualized antiretroviral treatment, and lipid-modifying techniques to reduce cardiovascular risks for HIV patients. It concludes by stressing the importance of risk profiling to identify and educate high-risk patients.
This study analyzed data from 780 COVID-19 patients in Indonesia to examine the association between vitamin D status and mortality. The results showed that most deaths occurred in older male patients with pre-existing conditions and low vitamin D levels. After controlling for other factors, low vitamin D status was strongly associated with higher COVID-19 mortality. Randomized controlled trials are needed to further investigate the role of vitamin D supplementation on COVID-19 outcomes.
The document discusses sepsis, including definitions, severity, statistics, emergency management, and treatment strategies. Key points include: recognizing sepsis early and treating it aggressively is important as mortality increases with each additional failing organ system; the "Sepsis Six" bundle includes administering antibiotics and fluids, maintaining oxygenation and circulation, and obtaining cultures within one hour; goal-directed resuscitation focusing on lactate clearance and ScvO2 normalization improves outcomes compared to usual care; corticosteroids may be considered for noradrenaline-resistant shock; source control through early intervention is important. The case study demonstrates application of these principles in a patient with severe sepsis.
This document discusses the application of precision medicine to Alzheimer's disease. It begins with background on the history and concepts of precision medicine and Alzheimer's disease. Precision medicine aims to personalize treatment based on individual characteristics like genetics and biomarkers. For Alzheimer's, precision medicine could help identify at-risk groups, understand the disease process, and develop targeted treatments. The role of genetics in precision medicine for Alzheimer's is discussed. Future directions include incorporating precision medicine into clinical trials to further advance personalized prevention and treatment of Alzheimer's disease.
Sandra Odebrechet and her team investigated the potential pathogenesis of schizophrenia through the Borna Disease Virus. They tested 27 schizophrenic and schizoaffective patients, as well as 27 healthy volunteer controls and family members with and without mood disorders, for traces of Borna Disease Virus RNA. Their methodology followed international safety guidelines to prevent contamination. They found BDV RNA in 44% of schizophrenic subjects, 50% of relatives without mental disorders, and 37.5% of relatives with mood disorders. This study provided more geographical variation than others by being conducted in Brazil.
This document provides an overview of chronic spontaneous urticaria (CSU), including its epidemiology, pathophysiology, clinical presentation, investigations, and management. It discusses the diagnosis and classification of urticaria and focuses on the diagnosis and investigation of CSU. Routine diagnostic measures for CSU include CBC, ESR or CRP, and eliminating possible causes such as medications or foods. Extended diagnostic testing may include allergy testing, infections screening, autoantibody testing such as the autologous serum skin test, and screening for underlying conditions. The gold standard treatment for CSU is non-sedating H1 antihistamines as monotherapy or in increased doses. Refractory cases may require the addition
This document discusses the importance of drug allergy testing, specifically for penicillin allergy. It reviews the steps for taking a medication allergy history and testing, including skin testing for penicillin. Avoiding antibiotics due to reported allergy can increase costs, treatment failure, infections, and resistance. Testing is important as up to 95% of patients reporting penicillin allergy may actually be able to tolerate it. The document reviews indications for challenge or desensitization, including using challenge for low-risk histories and desensitization when no alternative treatments exist. It provides an example case of a boy who has penicillin skin testing to safely receive treatment for an infection.
James Fingleton PhD thesis amended FINAL version 14th NovemberJames Fingleton
This thesis explores phenotypes of obstructive airways disease through cluster analysis of data from the New Zealand Respiratory Health Survey (NZRHS). The NZRHS involved over 1,000 participants and examined clinical characteristics, lung function, biomarkers and responses to bronchodilators and inhaled corticosteroids to identify phenotypes. Cluster analysis identified five distinct phenotypes that differ in pathophysiology and treatment response. The thesis aims to characterize the identified phenotypes and develop rules to allocate patients to the appropriate phenotype, in order to enable personalized treatment of obstructive airway diseases.
There is Time to Adjust. Aging as a Protective Factor for Autism-Crimson Publ...CrimsonPublishersGGS
There is Time to Adjust. Aging as a Protective Factor for Autism by Diego Iacono in Gerontology & Geriatrics studies
Autism spectrum disorder (ASD) is formally diagnosed before the age of 3 that is, when the central nervous system (CNS) is not yet completely formed, but it is mature enough to generate behavioural abnormalities in some individuals when compared to an age- matched group of typically developed children [1,2]. However, ASD is not a life-threating disease and children diagnosed with ASD age at the same rate as their peers. The possible detrimental or beneficial factors associated with aging in children affected by ASD are not fully known. Surprisingly, the amount of peer-reviewed medical and scientific international literature published on the topic of aging with autism is quite modest and sporadic [3]. The scarcity of aging-ASD investigations derives from the lower level of attention, and related funding opportunities, from the major public and private funding agencies for research across the globe
This document provides a summary of Prof. Hakan Erdem's professional experience and qualifications. It outlines that he has over 20 years of experience in infectious diseases and clinical microbiology. He founded ID-IRI, an international clinical research platform, in 2008. He has published over 100 papers in international journals and textbook sections on topics like brucellosis, tuberculosis, and community-acquired pneumonia. He has worked in leadership roles at several hospitals and universities in Turkey.
A systematic review of the association between ptb and the development of chr...EArl Copina
This systematic review examined evidence for an association between pulmonary tuberculosis (PTB) and the development of chronic airflow obstruction (CAO). The review included 19 studies comprising 1 case series, 3 case-control studies, 4 cohort studies, and 8 cross-sectional studies involving over 10,000 subjects total. The majority of studies, including 3 large population-based surveys, found a significant positive association between PTB and CAO, with odds ratios ranging from 1.37 to 2.94. While causality cannot be proven, the evidence confirms that a history of PTB is independently associated with CAO.
This document summarizes and discusses several journal articles and medical cases. It includes the following:
- A study of over 224,000 rapid strep tests and throat cultures finding non-Group A strep in 3.1% of cultures.
- Nitrofurantoin being an effective treatment for lower urinary tract infections caused by ESBL bacteria.
- A review of Lyme disease epidemiology and management in Northeast Ohio.
- Guidelines for rabies post-exposure prophylaxis in dog bites, noting most dog bites do not require treatment.
- A study finding asymptomatic carriage of diarrheagenic E. coli equally in symptomatic and asymptomatic patients.
- A study on influenza vaccine efficacy finding
1) The document discusses guidelines for treating Clostridium difficile (C. diff) infection in children, including risk factors, disease classification, and treatment recommendations.
2) Treatment for initial mild or moderate C. diff includes oral metronidazole or vancomycin for 10 days, while severe cases recommend oral vancomycin with or without IV metronidazole for 10-14 days.
3) Recurrent cases suggest pulsed oral vancomycin dosing or alternative therapies like fidaxomicin or nitazoxanide under infectious disease guidance.
This collaboration between Albert Einstein College of Medicine and Cardozo Law builds upon the long history and rich clinical experience of the Montefiore-Einstein bioethics consultation service. With integrated courses designed specifically for the bioethics curriculum, our program provides personal enrichment while equipping you with the skills you need to navigate the nuances of policy and regulation in a changing healthcare environment.
Format 2016: what is new in allergic & diseases respiratory 2016.Envicon Medical Srl
The document summarizes recent research in the fields of allergic and respiratory diseases from 2016. It includes summaries of multiple studies related to topics like drug allergy, food allergy, asthma, allergic rhinitis, and infectious respiratory diseases. The studies examined issues such as improving the effectiveness of penicillin allergy de-labeling through skin and drug testing, using drug provocation tests to diagnose non-immediate reactions to antibiotics like amoxicillin in children, and establishing reference doses for precautionary food labeling. One survey also found discrepancies between what health care professionals believe indicates risks of cross-contamination and what they consider to be best practices for precautionary allergen labeling.
The ERS/ATS Task Force guidelines provide an updated definition of severe asthma, discuss severe asthma phenotypes, and make recommendations for evaluating and treating severe asthma.
When asthma diagnosis is confirmed and comorbidities addressed, severe asthma is defined as requiring high-dose inhaled corticosteroids plus a second controller and/or systemic corticosteroids to control asthma or asthma that remains uncontrolled despite this therapy.
Severe asthma is heterogeneous, with phenotypes including eosinophilic asthma. Recommendations include using sputum eosinophil counts and exhaled nitric oxide to guide therapy, as well as treating with anti-IgE antibody, methotrexate, macrolide antibiotics, antifungal agents and
The ERS/ATS Task Force guidelines provide an updated definition of severe asthma, discuss severe asthma phenotypes, and make recommendations for evaluating and treating severe asthma.
When asthma diagnosis is confirmed and comorbidities addressed, severe asthma is defined as requiring high-dose inhaled corticosteroids plus a second controller and/or systemic corticosteroids to control asthma or asthma that remains uncontrolled despite this therapy.
Severe asthma is heterogeneous, with phenotypes including eosinophilic asthma. Recommendations include using sputum eosinophil counts and exhaled nitric oxide to guide therapy, as well as treating with anti-IgE antibody, methotrexate, macrolide antibiotics, antifungal agents and
Chikungunya as a Cause of Acute Febrile Illness in Southern Sri LankaYan'an Hou
This study investigated chikungunya virus (CHIKV) as a cause of acute febrile illness in southern Sri Lanka during a 2007 outbreak. Researchers enrolled 797 patients presenting with undifferentiated fever at a large hospital. Serology identified acute CHIKV infection in 3.5% of patients without acute dengue virus infection, and PCR/viral isolation confirmed 64.3% of these cases. Sequencing of CHIKV isolates showed they possessed the E1-226A residue and were closely related to contemporary Sri Lankan and Indian isolates. Except for more frequent and persistent musculoskeletal symptoms, acute CHIKV mimicked dengue and other febrile illnesses clinically. Only 1.
Frequency of migraine headaches in patients with fibromyalgiaPaul Coelho, MD
- The study evaluated the frequency of migraine headaches in a large cohort of patients with fibromyalgia using a brief migraine screening tool.
- Of the 1730 patients with fibromyalgia who completed the survey, 966 (55.8%) met the criteria for migraine headaches based on the screening tool.
- Several comorbid conditions such as depression, anxiety, chronic fatigue syndrome, and irritable bowel syndrome were found to be significantly more common in those fibromyalgia patients who also met the criteria for migraines.
The number of existing functional somatic syndromes (fs ss) is an important r...Paul Coelho, MD
The study tested the hypothesis that the number of existing functional somatic syndromes (FSSs; e.g. fibromyalgia, chronic fatigue syndrome, irritable bowel syndrome) predicts the development of new FSSs. The study found that the incidence of a new FSS increased with the number of pre-existing (antecedent) FSSs in both cases (women with interstitial cystitis) and controls. Specifically, the risk of a new FSS was highest for individuals with 3 or more antecedent FSSs. Logistic regression showed that the number of antecedent FSSs significantly predicted new FSSs even after accounting for other risk factors. This supports the idea that FSSs are linked
A Prospective Observational Study of Zinc As Adjunct Therapy In Pediatric Pop...iosrjce
IOSR Journal of Dental and Medical Sciences is one of the speciality Journal in Dental Science and Medical Science published by International Organization of Scientific Research (IOSR). The Journal publishes papers of the highest scientific merit and widest possible scope work in all areas related to medical and dental science. The Journal welcome review articles, leading medical and clinical research articles, technical notes, case reports and others.
The document discusses cardiovascular disease in HIV patients. It notes that HIV can predispose patients to cardiovascular diseases, and that antiretroviral drugs used to treat HIV can impact lipid profiles. Elderly HIV patients are also more susceptible to comorbidities like cardiovascular disease. The document outlines the need for primary, secondary, and tertiary prevention strategies like education, individualized antiretroviral treatment, and lipid-modifying techniques to reduce cardiovascular risks for HIV patients. It concludes by stressing the importance of risk profiling to identify and educate high-risk patients.
This study analyzed data from 780 COVID-19 patients in Indonesia to examine the association between vitamin D status and mortality. The results showed that most deaths occurred in older male patients with pre-existing conditions and low vitamin D levels. After controlling for other factors, low vitamin D status was strongly associated with higher COVID-19 mortality. Randomized controlled trials are needed to further investigate the role of vitamin D supplementation on COVID-19 outcomes.
The document discusses sepsis, including definitions, severity, statistics, emergency management, and treatment strategies. Key points include: recognizing sepsis early and treating it aggressively is important as mortality increases with each additional failing organ system; the "Sepsis Six" bundle includes administering antibiotics and fluids, maintaining oxygenation and circulation, and obtaining cultures within one hour; goal-directed resuscitation focusing on lactate clearance and ScvO2 normalization improves outcomes compared to usual care; corticosteroids may be considered for noradrenaline-resistant shock; source control through early intervention is important. The case study demonstrates application of these principles in a patient with severe sepsis.
This document discusses the application of precision medicine to Alzheimer's disease. It begins with background on the history and concepts of precision medicine and Alzheimer's disease. Precision medicine aims to personalize treatment based on individual characteristics like genetics and biomarkers. For Alzheimer's, precision medicine could help identify at-risk groups, understand the disease process, and develop targeted treatments. The role of genetics in precision medicine for Alzheimer's is discussed. Future directions include incorporating precision medicine into clinical trials to further advance personalized prevention and treatment of Alzheimer's disease.
Sandra Odebrechet and her team investigated the potential pathogenesis of schizophrenia through the Borna Disease Virus. They tested 27 schizophrenic and schizoaffective patients, as well as 27 healthy volunteer controls and family members with and without mood disorders, for traces of Borna Disease Virus RNA. Their methodology followed international safety guidelines to prevent contamination. They found BDV RNA in 44% of schizophrenic subjects, 50% of relatives without mental disorders, and 37.5% of relatives with mood disorders. This study provided more geographical variation than others by being conducted in Brazil.
This document provides an overview of chronic spontaneous urticaria (CSU), including its epidemiology, pathophysiology, clinical presentation, investigations, and management. It discusses the diagnosis and classification of urticaria and focuses on the diagnosis and investigation of CSU. Routine diagnostic measures for CSU include CBC, ESR or CRP, and eliminating possible causes such as medications or foods. Extended diagnostic testing may include allergy testing, infections screening, autoantibody testing such as the autologous serum skin test, and screening for underlying conditions. The gold standard treatment for CSU is non-sedating H1 antihistamines as monotherapy or in increased doses. Refractory cases may require the addition
This document discusses the importance of drug allergy testing, specifically for penicillin allergy. It reviews the steps for taking a medication allergy history and testing, including skin testing for penicillin. Avoiding antibiotics due to reported allergy can increase costs, treatment failure, infections, and resistance. Testing is important as up to 95% of patients reporting penicillin allergy may actually be able to tolerate it. The document reviews indications for challenge or desensitization, including using challenge for low-risk histories and desensitization when no alternative treatments exist. It provides an example case of a boy who has penicillin skin testing to safely receive treatment for an infection.
James Fingleton PhD thesis amended FINAL version 14th NovemberJames Fingleton
This thesis explores phenotypes of obstructive airways disease through cluster analysis of data from the New Zealand Respiratory Health Survey (NZRHS). The NZRHS involved over 1,000 participants and examined clinical characteristics, lung function, biomarkers and responses to bronchodilators and inhaled corticosteroids to identify phenotypes. Cluster analysis identified five distinct phenotypes that differ in pathophysiology and treatment response. The thesis aims to characterize the identified phenotypes and develop rules to allocate patients to the appropriate phenotype, in order to enable personalized treatment of obstructive airway diseases.
There is Time to Adjust. Aging as a Protective Factor for Autism-Crimson Publ...CrimsonPublishersGGS
There is Time to Adjust. Aging as a Protective Factor for Autism by Diego Iacono in Gerontology & Geriatrics studies
Autism spectrum disorder (ASD) is formally diagnosed before the age of 3 that is, when the central nervous system (CNS) is not yet completely formed, but it is mature enough to generate behavioural abnormalities in some individuals when compared to an age- matched group of typically developed children [1,2]. However, ASD is not a life-threating disease and children diagnosed with ASD age at the same rate as their peers. The possible detrimental or beneficial factors associated with aging in children affected by ASD are not fully known. Surprisingly, the amount of peer-reviewed medical and scientific international literature published on the topic of aging with autism is quite modest and sporadic [3]. The scarcity of aging-ASD investigations derives from the lower level of attention, and related funding opportunities, from the major public and private funding agencies for research across the globe
This document provides a summary of Prof. Hakan Erdem's professional experience and qualifications. It outlines that he has over 20 years of experience in infectious diseases and clinical microbiology. He founded ID-IRI, an international clinical research platform, in 2008. He has published over 100 papers in international journals and textbook sections on topics like brucellosis, tuberculosis, and community-acquired pneumonia. He has worked in leadership roles at several hospitals and universities in Turkey.
A systematic review of the association between ptb and the development of chr...EArl Copina
This systematic review examined evidence for an association between pulmonary tuberculosis (PTB) and the development of chronic airflow obstruction (CAO). The review included 19 studies comprising 1 case series, 3 case-control studies, 4 cohort studies, and 8 cross-sectional studies involving over 10,000 subjects total. The majority of studies, including 3 large population-based surveys, found a significant positive association between PTB and CAO, with odds ratios ranging from 1.37 to 2.94. While causality cannot be proven, the evidence confirms that a history of PTB is independently associated with CAO.
This document summarizes and discusses several journal articles and medical cases. It includes the following:
- A study of over 224,000 rapid strep tests and throat cultures finding non-Group A strep in 3.1% of cultures.
- Nitrofurantoin being an effective treatment for lower urinary tract infections caused by ESBL bacteria.
- A review of Lyme disease epidemiology and management in Northeast Ohio.
- Guidelines for rabies post-exposure prophylaxis in dog bites, noting most dog bites do not require treatment.
- A study finding asymptomatic carriage of diarrheagenic E. coli equally in symptomatic and asymptomatic patients.
- A study on influenza vaccine efficacy finding
1) The document discusses guidelines for treating Clostridium difficile (C. diff) infection in children, including risk factors, disease classification, and treatment recommendations.
2) Treatment for initial mild or moderate C. diff includes oral metronidazole or vancomycin for 10 days, while severe cases recommend oral vancomycin with or without IV metronidazole for 10-14 days.
3) Recurrent cases suggest pulsed oral vancomycin dosing or alternative therapies like fidaxomicin or nitazoxanide under infectious disease guidance.
This collaboration between Albert Einstein College of Medicine and Cardozo Law builds upon the long history and rich clinical experience of the Montefiore-Einstein bioethics consultation service. With integrated courses designed specifically for the bioethics curriculum, our program provides personal enrichment while equipping you with the skills you need to navigate the nuances of policy and regulation in a changing healthcare environment.
Format 2016: what is new in allergic & diseases respiratory 2016.Envicon Medical Srl
The document summarizes recent research in the fields of allergic and respiratory diseases from 2016. It includes summaries of multiple studies related to topics like drug allergy, food allergy, asthma, allergic rhinitis, and infectious respiratory diseases. The studies examined issues such as improving the effectiveness of penicillin allergy de-labeling through skin and drug testing, using drug provocation tests to diagnose non-immediate reactions to antibiotics like amoxicillin in children, and establishing reference doses for precautionary food labeling. One survey also found discrepancies between what health care professionals believe indicates risks of cross-contamination and what they consider to be best practices for precautionary allergen labeling.
The ERS/ATS Task Force guidelines provide an updated definition of severe asthma, discuss severe asthma phenotypes, and make recommendations for evaluating and treating severe asthma.
When asthma diagnosis is confirmed and comorbidities addressed, severe asthma is defined as requiring high-dose inhaled corticosteroids plus a second controller and/or systemic corticosteroids to control asthma or asthma that remains uncontrolled despite this therapy.
Severe asthma is heterogeneous, with phenotypes including eosinophilic asthma. Recommendations include using sputum eosinophil counts and exhaled nitric oxide to guide therapy, as well as treating with anti-IgE antibody, methotrexate, macrolide antibiotics, antifungal agents and
The ERS/ATS Task Force guidelines provide an updated definition of severe asthma, discuss severe asthma phenotypes, and make recommendations for evaluating and treating severe asthma.
When asthma diagnosis is confirmed and comorbidities addressed, severe asthma is defined as requiring high-dose inhaled corticosteroids plus a second controller and/or systemic corticosteroids to control asthma or asthma that remains uncontrolled despite this therapy.
Severe asthma is heterogeneous, with phenotypes including eosinophilic asthma. Recommendations include using sputum eosinophil counts and exhaled nitric oxide to guide therapy, as well as treating with anti-IgE antibody, methotrexate, macrolide antibiotics, antifungal agents and
This document presents a debate on whether preschool children experiencing acute wheezing episodes should be treated with oral corticosteroids (OCS). The pro side argues that many preschool children with recurrent wheezing develop atopic disease and sensitization which predicts increased risk of asthma and response to OCS therapy. Studies have shown heterogeneity in design and populations making it difficult to make definitive recommendations against OCS use. The con side argues that most studies have not demonstrated beneficial effects of OCS for acute wheezing in preschool children. Repeated OCS bursts may also be associated with adverse effects. Both sides agree more efficacy trials are needed targeting phenotypes likely to respond to OCS.
Running head: PICOT 1
PICOT 6
PICOT STATEMENT AND LITERATURE SEARCH
Student’s Name: Idalmis Espinosa
Institutional Affiliation: Grand Canyon University
Date: 04/23/17
EBS PROCESS
The nurses ought to measure the blood pressure of the patients depending on the evidence-based process to ensure accuracy. Accurate measurements are a crucial factor in the effective treatment of diabetes, pediatric and dialysis. The method used to measure the blood pressure in children is different from that employed in adults. In children, the process includes an auscultatory strategy that compares the results with those in the oscillometric tool.
PICOT STATEMENT
P – Population: Children about 8 to 15 years with a clinical diagnosis of diabetes, pediatric and dialysis.
I – Intervention: The subjects will be randomized to have management in different time frames of 2, 4, 6 and eight weeks.
C – Comparison: A standardized subject would be used as a control to make active comparisons. This strategy will help us to minimize effects related to not attending the clinic.
O – Outcome: Changes in the blood pressure and blood sugar level.
T – Time: The outcome would be assessed weekly for eight weeks.
Chavers, B. M., Li, S., Collins, A. J., & Herzog, C. A. (2002). Cardiovascular disease in pediatric chronic dialysis patients. Kidney international.
According to Chavers and the rest, there is little information regarding the mortality rate of the children with diabetes and renal diseases. The study evaluated the mortality rate in children suffering from pediatric chronic dialysis. Children of ages ranging from 2 to 17 years were identified from the data system of the United States Renal Data system. A sum of 1500 children was eligible for the enclosure. 31 percent of the kids developed cardiac related diseases, while the rest developed other conditions that are related to either diabetes or pediatric dialysis. The study concluded that cardiovascular disease is the primary cause of child mortality and morbidity in pediatric chronic dialysis.
Brenner, B. M., Cooper, M. E., & Shahinfar, S. (2001). Effects of losartan on renal and cardiovascular outcomes in patients with type 2 diabetes and nephropathy. New England Journal of Medicine.
Brenner et al. contend that nephropathy is the leading source of renal disease. The researchers evaluated the function of the receptor antagonist in the type 2 diabetes patients and nephropathy. A sum of 1500 patients was randomly selected for assessment over the period of 3 years. The receptor antagonist indicated substantial benefits to the renal in the type 2 diabetes patients and nephropathy. The researchers, therefore, concluded that nephropathy could ca ...
1) Allergic rhinitis (AR) is a common condition that affects millions of people in the US. It imposes a significant economic burden due to direct and indirect medical costs.
2) The diagnosis of AR can often be made based on a patient's symptoms of sneezing, rhinorrhea, nasal congestion, and watery eyes. It is important to differentiate between seasonal and perennial AR.
3) Other conditions like sinusitis and non-allergic rhinitis should also be considered in patients with nasal symptoms. Examination may reveal signs of conditions like asthma that commonly accompany AR.
This document summarizes several articles on asthma heterogeneity and treatment. It discusses how asthma has many origins leading to airway inflammation, and both genetic and environmental factors influence disease pathogenesis. Origins of childhood asthma often have atopic roots, and the infant microbiome may impact susceptibility. In adults, asthma commonly co-occurs with other conditions like obesity. Biomarkers can help identify asthma phenotypes to select the right biologic treatment, though they are not definitive on their own. Caregiver depression also influences childhood asthma through socioeconomic, familial and biological pathways and mechanisms.
This document summarizes a study analyzing data from 14 countries that participated in the Burden of Obstructive Lung Disease (BOLD) study to describe characteristics of COPD in never smokers and identify possible risk factors. The study found that among 4,291 never smokers, 6.6% had mild COPD and 5.6% had moderate to severe COPD. Never smokers comprised 23.3% of those with moderate to severe COPD. Predictors of COPD in never smokers included older age, lower education levels, occupational exposures, childhood respiratory diseases, and abnormal BMI. The study confirms that never smokers represent a substantial proportion of COPD cases and suggests additional risk factors beyond smoking.
The document summarizes a clinical trial that compared the effectiveness of adding montelukast (Singulair) versus salmeterol/fluticasone (Advair) or increasing the dose of fluticasone for children with uncontrolled asthma on low-dose inhaled corticosteroids. The BADGER trial found that adding salmeterol to fluticasone or increasing the fluticasone dose resulted in better asthma control compared to adding montelukast. However, some children responded better to montelukast. The document concludes that while several options exist, the patient's current level of control indicates a need to step up therapy beyond low-dose inhaled corticosteroids alone
This document summarizes an article from the journal Asthma in General Practice. The article investigates the underpresentation of shortness of breath symptoms to general practitioners in the general population without a confirmed diagnosis of obstructive airways disease. Of 285 people who experienced shortness of breath in the past year, only 93 (33%) had ever consulted their GP for this. Neither perception of symptoms nor psychological factors could explain the underpresentation. A random sample of over 1,000 people was screened for respiratory symptoms, and those reporting shortness of breath were studied further. While shortness of breath was common, most people did not seek medical help for it. This suggests that underpresentation, not underdiagnosis, contributes significantly to the
The document discusses the evaluation and management of difficult or severe asthma. It notes that in evaluating these patients, it is important to first establish an accurate diagnosis of asthma through objective measures like spirometry before and after bronchodilation. Studies have found that a substantial percentage of patients diagnosed with difficult asthma were later found to have an alternative or incorrect diagnosis after thorough evaluation. Assessing and addressing comorbidities, adherence, environmental factors, and phenotypes is also important for optimizing treatment of difficult asthma. Difficult asthma can be divided into cases where underlying problems can be addressed versus true therapy-resistant severe asthma.
ASSESSING DEMOGRAPHIC DISPARITIES IN UTILIZATION OF INHALED CORTICOSTEROIDS A...gpartha85
This study analyzed demographic disparities in the utilization of inhaled corticosteroids (ICS) among patients with persistent asthma using data from the National Asthma Survey. The results showed that over half of patients did not use ICS. African Americans and uninsured patients had significantly lower odds of using ICS compared to whites and insured patients respectively. Multivariate analysis controlling for demographic factors found that African Americans had lower odds than whites of using ICS, potentially due to lack of access to care and inconsistencies in treatment guidelines. Insurance status was also a significant predictor of ICS use, with insured patients having higher odds of use.
This document discusses a study on the use of Phatak's Repertory in treating respiratory diseases in children. 30 cases of acute and chronic respiratory diseases in children of different ages and socioeconomic backgrounds were treated over 12 months. The cases were analyzed and medicines selected using Phatak's Repertory. The results found 53.3% of cases recovered, 30% improved, and 16.6% saw no improvement. While the repertory has limitations due to fewer rubrics and remedies compared to other repertories, it was found to be useful in selecting similimums in pediatric respiratory cases in the shortest time based on its inclusion of modalities, causations, and pathological generals.
This document discusses a study on using Phatak's Repertory to treat respiratory diseases in children. It provides background on repertories and Phatak's Repertory. The study aimed to evaluate the scope and limitations of Phatak's Repertory in treating children's respiratory diseases. 30 cases of acute and chronic respiratory diseases in children were treated using Phatak's Repertory. Most cases were school-aged children from average socioeconomic families with a family history of respiratory or other miasmatic diseases. Results showed improvement in most acute cases and recovery in many chronic cases.
Effects of moderate doses of vitamin A as an adjunct to the treatment of pneu...ISAMI1
Effects of moderate doses of vitamin A as an adjunct to the treatment of pneumonia in underweight and normal-weight children: a randomized, double-blind, placebo-controlled trial
This document provides clinical practice guidelines for the management of community-acquired pneumonia (CAP) in infants and children older than 3 months. It includes recommendations on site-of-care management decisions, diagnostic testing, antimicrobial and surgical therapy, and prevention. The expert panel developed evidence-based guidelines to assist clinicians in decreasing the morbidity and mortality of CAP in children. The guidelines cover practical questions of diagnosis and treatment of CAP in both outpatient and inpatient settings.
Benjamin Littenberg and colleagues conducted a meta-analysis of 59 studies involving nearly 56,000 patients to analyze the accuracy and inconsistencies of rapid strep tests. They found that while the tests are generally excellent at correctly diagnosing patients, their ability to accurately return negative results varied between 70-95%. Key factors influencing a test's accuracy included the patient's age, testing location, and whether the study was sponsored by a manufacturer. The analysis revealed inconsistencies in test performance but not the underlying reasons. Littenberg concludes more research is needed to address sources of variability and improve data on rapid strep test accuracy.
This document provides guidelines from the American Heart Association and American Thoracic Society for the diagnosis, evaluation, and treatment of pulmonary hypertension in children. It was created by an expert panel through extensive literature review and discussion. The guidelines aim to address gaps in knowledge about pediatric pulmonary hypertension, which differs from the adult form in causes, natural history, and response to treatment. The document presents recommendations to help clinicians manage this condition, though many are based on expert opinion due to the lack of pediatric clinical trials. The goals are to improve care for children with pulmonary hypertension.
Indice Predictor de Asma en edad pediátricaYan Giraldo G
This document describes a study that developed two indices to predict the risk of asthma in young children with recurrent wheezing. The researchers used data from the Tucson Children's Respiratory Study to create a stringent index and a loose index based on combinations of wheezing frequency, family history, eczema, allergic rhinitis, eosinophilia, and wheezing apart from colds. Children who met the criteria for the loose index had 2.6 to 5.5 times higher risk of active asthma between ages 6-13. Those who met the stringent index criteria had 4.3 to 9.8 times higher risk. 59% of children with a positive loose index and 76% of those with a positive
This document provides clinical practice guidelines for the management of community-acquired pneumonia (CAP) in infants and children older than 3 months. It was created by an expert panel to assist clinicians in caring for children with CAP. The guidelines address site-of-care management decisions, diagnostic testing, antimicrobial and surgical therapy, and prevention. Specific recommendations are made regarding criteria for hospitalization versus outpatient treatment and appropriate diagnostic tests. The goal is to decrease morbidity and mortality from CAP in children by presenting management strategies that can be applied based on individual cases.
Cor pulmonale, also known as pulmonary heart disease, is a type of heart disease where the right side of the heart enlarges and fails over time due to high blood pressure in the lungs or pulmonary hypertension. It is usually caused by lung diseases like chronic obstructive pulmonary disease (COPD) that result in low oxygen levels and resistance within the pulmonary arteries of the lungs. The enlarged right ventricle must work harder to pump blood into the lungs, causing it to thicken and eventually fail if left untreated.
Peptic ulcers develop in the lining of the stomach or small intestine. Common causes are infection by H. pylori bacteria and use of pain medications like ibuprofen. Symptoms include stomach pain that is worse when the stomach is empty. Diagnosis involves tests to detect H. pylori infection and endoscopy to view the digestive tract. Treatment eliminates the bacteria with antibiotics if present, reduces acid production, and promotes healing with proton pump inhibitors or acid blockers. Preventive measures include careful use of pain medications and protecting against infections.
Pancreatitis is inflammation of the pancreas that can be acute or chronic. It occurs when digestive enzymes in the pancreas are activated and damage pancreatic cells. Common causes include alcoholism, gallstones, certain medications, abdominal injuries, and genetic factors. Symptoms vary but often include abdominal pain that worsens with eating as well as nausea and vomiting. Diagnosis involves blood tests, imaging scans, and endoscopy. Treatment focuses on relieving symptoms, treating underlying causes, and managing complications. To prevent pancreatitis, avoiding excessive alcohol consumption and following a low-fat diet can help reduce risk.
Irritable bowel syndrome (IBS) is a functional gastrointestinal disorder defined by abdominal pain and changes in bowel habits. The causes are unclear but may involve stress, infection, or brain-gut interactions. Symptoms include abdominal pain, gas, bloating, and diarrhea or constipation. Diagnosis is based on symptoms, and tests rule out other conditions. Treatment focuses on lifestyle changes like diet, exercise, and stress relief. Medications may help control symptoms but no single treatment works for everyone with this common disorder.
Inflammatory bowel disease (IBD) describes disorders that involve chronic inflammation of the digestive tract, including ulcerative colitis and Crohn's disease. The exact cause is unknown but is likely related to malfunctions of the immune system. Symptoms vary in severity from mild to severe and include diarrhea, abdominal pain, bleeding, and weight loss. Diagnosis involves blood tests, endoscopy, imaging, and ruling out other potential causes. Treatment aims to reduce inflammation and includes anti-inflammatory drugs, immunosuppressants, antibiotics, and surgery in some cases.
Helicobacter pylori is a type of bacteria that infects the stomach and is the most common cause of gastritis and peptic ulcers worldwide. Infection is very common and increases with age. H. pylori bacteria can be transmitted through direct contact with infected feces or vomit, or through contaminated food or water. While most infected people never show symptoms, potential symptoms include abdominal pain, nausea, loss of appetite, and weight loss. Diagnosis involves tests of the blood, breath, or stool to detect H. pylori. Treatment consists of antibiotics along with medication to reduce stomach acid in order to kill the bacteria and allow the stomach lining to heal.
Gastroesophageal reflux disease (GERD) occurs when stomach acid returns up into the esophagus and causes irritation. It is common for people to experience acid reflux occasionally, but GERD is when it occurs at least twice a week or more severely once a week. Risk factors include obesity, pregnancy, smoking, and certain medications. Symptoms include heartburn, nausea, coughing, and sore throat. Diagnosis is usually based on symptoms, but tests like endoscopy or pH monitoring can be done. Treatment involves lifestyle changes like losing weight, avoiding foods and drinks that trigger symptoms, and medications to reduce acid production. Surgery may be an option for severe cases that do not improve with other treatments.
Gastroenteritis is inflammation of the stomach and intestines that is usually caused by viruses, bacteria, or parasites. Common symptoms include diarrhea, nausea, vomiting, and abdominal pain. While generally self-limiting, gastroenteritis can cause dehydration, which is more common and dangerous in infants and young children. Oral rehydration solutions are the recommended treatment for mild to moderate dehydration. Prevention involves proper food handling and drinking clean water, especially when traveling.
1. Gastrointestinal stromal tumors (GIST) are rare tumors that can develop in the wall of the gastrointestinal tract and can be cancerous (malignant) or non-cancerous (benign). Risk factors include inherited genetic mutations and rare genetic syndromes.
2. Symptoms of GIST tumors include blood in stool or vomit, abdominal pain, fatigue, difficulty swallowing, and feeling full after eating small amounts of food. Diagnosis involves physical exams, CT scans, MRI scans, endoscopic ultrasounds, and biopsies of suspicious tissue.
3. Treatment depends on the stage and location of the tumor and may involve surgical resection as the only potentially curative treatment
1. Gastritis is inflammation of the lining of the stomach that is usually caused by Helicobacter pylori infection or excessive alcohol or drug use. It can be acute or chronic.
2. Common causes include H. pylori infection, NSAIDs, alcohol, bile reflux, autoimmune disorders, and stress. Symptoms may include abdominal pain, nausea, vomiting, and black stools from bleeding.
3. Diagnosis involves blood tests, endoscopy, and stool tests. Treatment focuses on eliminating the cause, using antacids or other drugs to reduce stomach acid, and antibiotics to treat H. pylori infection. Preventing overuse of NSAIDs and limiting alcohol and sp
1. Esophageal motor disorders are alterations in the peristaltic activity of the esophageal body and/or functioning of the sphincters. There are primary motor disorders that affect the esophageal body and lower esophageal sphincter including achalasia, diffuse esophageal spasm, and hypercontractile disorders.
2. The etiology of spastic motor disorders is divided into primary and secondary causes. The primary causes include diffuse esophageal spasm, nutcracker esophagus, and hypertensive lower esophageal sphincter. The pathophysiology involves alterations in nitric oxide synthesis and degradation affecting normal peristalsis.
3. Symptoms include chest pain, dysphagia
Dyspepsia, also known as indigestion, refers to discomfort or pain in the upper abdomen that is often caused by eating. Common symptoms include pain, swelling, heartburn, and nausea. While the specific cause is unknown in most cases, potential causes include stress, medications like aspirin, Helicobacter pylori bacteria, smoking, alcohol, spicy or greasy foods, and large meal sizes. Diagnosis involves tests like abdominal ultrasounds and endoscopies of the esophagus, stomach, and duodenum. Treatment focuses on diet changes, antibiotics to eliminate H. pylori if present, and medications to reduce acid like omeprazole. Prevention includes relaxing after
This document discusses diseases of the rectum and anus. It begins with definitions and explains that these diseases are commonly seen in primary care. It describes some of the main diseases including their causes, symptoms, and risk factors. Some of the diseases covered include hemorrhoids, anal fissures, abscesses, and anal cancer. The document discusses how these diseases are diagnosed, often through examination with an anoscope or sigmoidoscope. It also outlines treatments for some conditions like surgery, radiation therapy, and chemotherapy for anal cancer. Lastly, it discusses some ways to prevent diseases like avoiding risk factors, screening high risk patients, and vaccinations to prevent HPV infections.
The document discusses diseases of the mouth cavity. It defines the mouth and its functions, and describes common mouth problems like cold sores, canker sores, and infections. Diagnosis involves examination by a dentist, and treatment depends on the specific problem but may include cleaning, surgery, or maintenance visits. Prevention strategies include avoiding tobacco and excessive alcohol, eating fruits and vegetables, protecting lips from sun, and regular dental exams.
The document discusses digestive hemorrhage, also known as gastrointestinal bleeding. It defines high and low hemorrhages based on their origin in the digestive tract. Some common causes of digestive hemorrhage include anal fissures, hemorrhoids, ulcers, and cancers of the digestive system. Symptoms include vomiting blood or black tarry stools. Diagnosis involves endoscopy, imaging tests, or surgery depending on the location of bleeding. Treatment focuses on stabilization, determining the cause through endoscopy, and addressing the specific condition causing the hemorrhage. Prevention strategies target those with risk factors like ulcers, cirrhosis, or who take anti-inflammatory drugs.
1. Diarrhea is defined as more frequent bowel movements with soft and liquid stools. It is usually caused by viruses, bacteria, parasites, certain foods, medications, and other digestive disorders.
2. Symptoms of diarrhea include soft, watery stools, abdominal cramps, pain, fever, blood in stool, swelling, and urgency. It can cause dehydration, especially in children.
3. Doctors may perform blood tests, stool analysis, and imaging tests to diagnose the cause. Treatment depends on the cause but often involves replacing fluids and electrolytes, antibiotics if bacteria is involved, and managing any underlying conditions.
Constipation is defined as having less than three bowel movements per week or hard stools that are difficult to pass. Common causes include low-fiber diets, lack of physical activity, certain medications, and ignoring the urge to have a bowel movement. Diagnosis involves physical exams, blood tests, and imaging tests of the colon and rectum. Treatment begins with lifestyle changes like increasing fiber intake and exercise. If those don't help, doctors may prescribe laxatives, stool softeners, or suppositories to relieve constipation symptoms.
Sleep apnea is a potentially serious sleep disorder where breathing stops and starts repeatedly during sleep. There are three main types - obstructive, central, and complex. Risk factors include being overweight, having a narrow throat, and certain facial structures. Symptoms include loud snoring, waking with shortness of breath, and daytime sleepiness. Diagnosis involves a physical exam and polysomnogram sleep test. Treatment options include lifestyle changes, CPAP devices, dental devices, and sometimes surgery. Losing weight and avoiding alcohol can help prevent sleep apnea.
1. Pulmonary tuberculosis is a chronic infectious disease caused by the bacterium Mycobacterium tuberculosis, which usually affects the lungs. It spreads through the air when people who are sick with TB cough, sneeze or speak.
2. Symptoms include a persistent cough lasting more than two weeks, coughing up blood, weight loss, fever, night sweats and fatigue. Diagnosis involves physical exams, chest x-rays, sputum tests and tuberculosis skin tests.
3. Treatment involves a multi-drug regimen administered by a tuberculosis specialist to prevent drug resistance. Patients are advised to rest at home and cover their mouth when coughing to prevent spreading the disease until treatment has reduced their contagious
1. Pulmonary embolism is caused by a blood clot in the lung, usually formed in the deep veins of the lower limbs. It is a leading cause of preventable death in hospitalized patients.
2. Symptoms include shortness of breath, chest pain, increased heart rate, coughing up blood, and fainting. Diagnosis involves assessing risk factors and test like D-dimer and CT scan.
3. Treatment involves anticoagulant drugs to dissolve clots initially by injection and then orally for 3-6 months. Prevention focuses on continuing anticoagulants, lifestyle changes like exercise and diet, wearing compression stockings, and early movement after surgery.
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Asthma
1. Heterogeneity of severe asthma in childhood: Confirmation
by cluster analysis of children in the National Institutes of
Health/National Heart, Lung, and Blood Institute Severe
Asthma Research Program
Anne M. Fitzpatrick, PhD,a
W. Gerald Teague, MD,b
Deborah A. Meyers, PhD,c
Stephen P. Peters, MD, PhD,c
Xingnan Li,
PhD,c
Huashi Li, MS,c
Sally E. Wenzel, MD,d
Shean Aujla, MD,d
Mario Castro, MD,e
Leonard B. Bacharier, MD,e
Benjamin M. Gaston, MD,b
Eugene R. Bleecker, MD,c
and Wendy C. Moore, MD,c
for the National Institutes of Health/
National Heart, Lung, and Blood Institute Severe Asthma Research Program* Atlanta, Ga, Charlottesville, Va, Winston-Salem,
NC, Pittsburgh, Pa, and St Louis, Mo
Background: Asthma in children is a heterogeneous disorder
with many phenotypes. Although unsupervised cluster analysis
is a useful tool for identifying phenotypes, it has not been
applied to school-age children with persistent asthma across a
wide range of severities.
Objectives: This study determined how children with severe
asthma are distributed across a cluster analysis and how well
these clusters conform to current definitions of asthma severity.
Methods: Cluster analysis was applied to 12 continuous and
composite variables from 161 children at 5 centers enrolled in
the Severe Asthma Research Program.
ResultsFour clusters of asthma were identified. Children in
cluster 1 (n 5 48) had relatively normal lung function and less
atopy. Children in cluster 2 (n 5 52) had slightly lower lung
function, more atopy, and increased symptoms and medication
use. Cluster 3 (n 5 32) had greater comorbidity, increased
bronchial responsiveness, and lower lung function. Cluster 4
(n 5 29) had the lowest lung function and the greatest
symptoms and medication use. Predictors of cluster assignment
were asthma duration, the number of asthma controller
medications, and baseline lung function. Children with severe
asthma were present in all clusters, and no cluster corresponded
to definitions of asthma severity provided in asthma treatment
guidelines.
Conclusion: Severe asthma in children is highly heterogeneous.
Unique phenotypic clusters previously identified in adults can
also be identified in children, but with important differences.
Larger validation and longitudinal studies are needed to
determine the baseline and predictive validity of these
phenotypic clusters in the larger clinical setting. (J Allergy Clin
Immunol 2011;127:382-9.)
Key words: Allergic sensitization, asthma, severe asthma, asthma
guidelines, children, cluster analysis, lung function, phenotype
Asthma in children is a chronic, persistent disorder character-
ized by airway inflammation and episodic airflow obstruction in
response to specific triggers.1
Whereas some children with
asthma have intermittent symptoms that are improved with
short-acting bronchodilators, many have classic, persistent symp-
toms requiring daily treatment with inhaled corticosteroids
(ICSs).2,3
Children with severe asthma are differentiated by ongo-
ing symptoms and airway inflammation despite treatment with
high doses of ICSs and other controller medications.4-6
Although
the prevalence of severe asthma is low, these children have ex-
treme morbidity4,5
and account for 30% to 50% of all pediatric
asthma health care costs.7,8
Children with severe asthma are a challenging group of patients
who can be difficult to treat. Although national and international
guidelines from the Global Initiative for Asthma (GINA) and the
National Asthma Education and Prevention Program (NAEPP)
emphasize the importance of assessing asthma severity in children
From a
the Department of Pediatrics, Emory University School of Medicine, Atlanta; b
the
Department of Pediatrics, University of Virginia School of Medicine; c
the Center for
Human Genomics, Wake Forest University School of Medicine, Winston-Salem; d
the
University of Pittsburgh School of Medicine; and e
the Washington University School
of Medicine, St Louis.
*A complete listing of Severe Asthma Research Program investigators is provided in the
acknowledgments.
Supported by National Institutes of Health grants RO1 HL069170, RO1 HL069167, RO1
HL069174, RO1 HL69149, and RO1 HL091762 and in part by the Center for Devel-
opmental Lung Biology, Children’s Healthcare of Atlanta, and PHS grants UL1
RR025008, KL2 RR025009, TL1 RR025010, and UL1 RR024992 from the Clinical
and Translational Science Award Program, National Institutes of Health, National
Center for Research Resources.
Disclosure of potential conflict of interest: A. M. Fitzpatrick has received research
support from the National Heart, Lung, and Blood Institute Severe Asthma Research
Program. W. G. Teague is a speaker for Merck, has received research support from the
National Institutes of Health and the American Lung Association, and is a volunteer for
Not One More Life. D. A. Meyers has received research support from the National
Institutes of Health. S. P. Peters has received research support from the National
Institutes of Health, National Heart, Lung, and Blood Institute Severe Asthma
Research Program. M. Castro is a consultant for Electrocore, NKTT, Schering,
Asthmatx, and Cephalon; is on the advisory board for Genentech; is a speaker for
AstraZeneca, Boehringer-Ingelheim, Pfizer, Merck, and GlaxoSmithKline; has re-
ceived grants from Asthmatx, Amgen, Ception, Genentech, Medimmune, Merck,
Novartis, the National Institutes of Health, and GlaxoSmithKline; and has received
royalties from Elsevier. L. B. Bacharier has received honoraria from AstraZeneca and
has received honoraria from and is on the advisory board for Genentech, Glaxo-
SmithKline, Merck, Schering-Plough, and Aerocrine. B. M. Gaston has received
research support from the National Institutes of Health and has served as an expert
witness on the topic of exhaled nitric oxide for Apieron. E. R. Bleecker is an advisor
and consultant for Aerovance, AstraZeneca, Boehringer-Ingelheim, Genentech,
GlaxoSmithKline, Merck, Novartis, Pfizer, and Wyeth and has received research
support from Aerovance, Amgen, AstraZeneca, Boehringer-Ingelheim, Centocor,
Ception, Genentech, GlaxoSmithKline, the National Institutes of Health, Novartis,
Pfizer, and Wyeth. The rest of the authors have declared that they have no conflict of
interest.
Received for publication July 8, 2010; revised November 8, 2010; accepted for publica-
tion November 12, 2010.
Available online January 6, 2011.
Reprint requests: Anne M. Fitzpatrick, PhD, 2015 Uppergate Drive, Atlanta, GA 30322.
E-mail: anne.fitzpatrick@emory.edu.
0091-6749/$36.00
Ó 2011 American Academy of Allergy, Asthma & Immunology
doi:10.1016/j.jaci.2010.11.015
382
2. Abbreviations used
ATS: American Thoracic Society
GINA: Global Initiative for Asthma
ICS: Inhaled corticosteroid
LABA: Long-acting b-agonist
NAEPP: National Asthma Education and Prevention Program
NHLBI: National Heart, Lung, and Blood Institute
SARP: Severe Asthma Research Program
before the initiation of therapy, severe asthma is defined primarily
by lung function abnormalities, persistent symptoms, and exac-
erbations despite appropriate therapy.3,9
This approach underesti-
mates the phenotypic heterogeneity of the disorder10
and may
further lead to suboptimal asthma treatment, because the majority
of children with persistent asthma have relatively normal lung
function during symptom-free periods with abnormal pulmonary
function only during acute exacerbations.11,12
Indeed, FEV1 does
not correlate well with the magnitude of asthma symptoms in chil-
dren,13
and values less than 80% predicted have a low sensitivity
(approximately 40%) for distinguishing asthma severity in this
population.14
These findings suggest that more specific ap-
proaches are needed to differentiate asthma heterogeneity in chil-
dren to assess better the risk and impairment associated with the
disorder as well as to guide clinical asthma therapies.
Cluster analysis is an unsupervised analytical approach that is
useful in the refinement of pediatric asthma diagnosis and severity
assessments because of its ability to distinguish complex pheno-
types without a priori (and therefore biased) definitions of disease
severity.15-17
In adults with chronic obstructive pulmonary disease
and asthma,18,19
cluster analyses have revealed distinct pheno-
types of obstructive airway disease that may ultimately require
modified approaches for their identification and diagnosis as
well as different therapeutic interventions. Cluster analysis de-
rived from the Severe Asthma Research Program (SARP) of the
National Heart, Lung, and Blood Institute (NHLBI) has resulted
in 5 novel clusters of asthma phenotypes in adults that do not cor-
respond to the levels of asthma severity as outlined by current
guidelines.19
Although that study19
and others20
emphasized the
importance of age of asthma onset in distinguishing the asthma
clusters, no cluster analysis has been undertaken in childhood
asthma. Given the significant heterogeneity in children with
asthma, thepurpose of this study was to apply unsupervised cluster
analysis to a diverse sample of children enrolled in SARP to deter-
mine (1) whether phenotypic clusters that conform to established
definitions of severe and nonsevere asthma are identifiable in chil-
dren, and (2) how these clusters relate to definitions of asthma se-
verity as proposed by the American Thoracic Society (ATS),15
the
NAEPP,3
and GINA.9
Because children enrolled in SARP are
characterized with comprehensive phenotyping similar to the
adult subjects,4,21
we raised the question whether previously
identified clusters of early-onset asthma in adults19
would also
be detected in children with similar phenotypic characteristics.
METHODS
The SARP is an NHLBI-supported research program with recruitment of
children 6 to 17 years of age across 5 centers in the United States. Each of the
SARP centers is affiliated with a major university teaching program, and
children are recruited into SARP from the outpatient clinics and inpatient
hospital wards of those academic centers. As a result, children enrolled in
SARP are more likely to have difficult asthma and are representative of a
referral population of children who receive care at academic versus commu-
nity centers. The protocol was approved by each center’s institutional review
board. Informed consent was obtained from the legal guardians of each child,
and verbal and written consent was obtained from participating children.
All children 6 to 17 years of age who underwent standardized character-
ization in SARP were eligible for inclusion. Eligible children had never
smoked and had physician-diagnosed asthma and historical evidence of
bronchial hyperresponsiveness or at least 12% FEV1 bronchodilator revers-
ibility either at baseline or during an acute exacerbation. Children were clas-
sified as having severe asthma according to ATS workshop criteria (see this
article’s Table E1 in the Online Repository at www.jacionline.org).15
This def-
inition assumes that comorbid conditions have been treated or addressed and
that the patient is adherent with prescribed asthma treatment. Thresholds for
high-dose ICS were adjusted for children and defined as >_440 mg fluticasone
equivalent per day for children less than 12 years and >_880 mg of fluticasone
equivalent per day for children 12 to 17 years of age (see this article’s Table E2
in the Online Repository at www.jacionline.org).4
All children enrolled re-
ceived a stable dose of ICS for at least 6 months. All were stable at the time
of characterization with no signs of acute respiratory illnesses. Children pre-
senting to the SARP clinic with an acute worsening of asthma control were
treated accordingly and were reassessed at a later date.
Characterization procedures
Participants underwent comprehensive phenotypic characterization con-
sisting of questionnaires, serum IgE and eosinophil quantification, allergy skin
prick testing, and bronchial responsiveness to methacholine as previously
described.4,21
Exhaled nitric oxide was determined with both offline (Sievers
NOA 280-I; Ionic Instruments, Boulder, Colo) and online (NIOX; Aerocrine,
Solna, Sweden) methods in accordance with published recommendations.22
Spirometry (KoKo PDS; Ferraris, Louisville, Colo) was performed at baseline
and after bronchodilator reversibility testing with 4, 6, and 8 inhalations of al-
buterol sulfate (90 mg per inhalation) to determine the best response to short-
acting b-agonists. Lung volumes were measured with a body plethysmograph
(MedGraphics Elite Series; MEDGRAPHICS, St Paul, Minn). Spirometry
predicted values were obtained by using the equations of Wang et al,23
and ple-
thysmographic lung volume predicted values were obtained by using the
Crapo24
predicted equations.
Variable reduction
The entire SARP dataset provided more than 500 variables that were
reduced to 12 variables before cluster analysis. Continuous variables included
the duration of asthma in months, baseline FEV1 percent predicted, and the
best postbronchodilator FEV1 percent predicted. Categorical variables in-
cluded sex, race (white, black, or other) and ICS group (none, low-dose, or
high-dose). Semiquantitative variables included b-agonist use over the previ-
ous 3 months, the frequency of symptoms, the magnitude of atopic sensitiza-
tion, and exhaled nitric oxide quartile. Composite variables were derived from
binary or discrete questionnaire data and were developed by study physicians
with experience in the study and treatment of childhood asthma to cover the
broad spectrum of routine asthma assessment in the clinical setting (see this
article’s Table E3 in the Online Repository at www.jacionline.org).19
These
composite variables included the number of asthma controller medications
and health care use in the previous year. For the composite variable health
care use in the previous year, subjects were assigned a rank on the basis
of the most severe use reported by the individual. Further description and per-
formance of the variables for atopic sensitization and exhaled nitric oxide
quartile appears in this article’s Tables E4 and E5 in the Online Repository
at www.jacionline.org. All variables were equally weighted in the analysis.
Subjects with missing data were excluded.
Statistical analysis
Cluster analysis was performed with SAS version 9.1 (SAS Institute Inc,
Cary, NC) as previously described (see this article’s Methods section in the
J ALLERGY CLIN IMMUNOL
VOLUME 127, NUMBER 2
FITZPATRICK ET AL 383
3. Online Repository at www.jacionline.org).19
The Ward minimum-variance hi-
erarchical clustering method was performed by using an agglomerative (bot-
tom-up) approach and Ward linkage (see this article’s Fig E1 in the Online
Repository at www.jacionline.org). At each generation of clusters, samples
were merged into larger clusters to minimize and maximize with within-
subjects and between-subjects sum of squares, respectively. ANOVAwith Tu-
key post hoc testing and x2
tests were used to determine differences between
groups. To determine the strongest predictors of cluster assignment, stepwise
discriminant analysis of the cluster variables was performed with the Fisher25
method as previously described26
by using an F value entry probability of 0.05
and removal probability of 0.10. Cross-validation was performed by extracting
each case and treating it as test data against the remaining cases.
RESULTS
Results from 273 children (mean age 10 years) enrolled in
SARP across 4 centers in Atlanta, Ga, Winston-Salem, NC,
Pittsburgh, Pa, St Louis, Mo, and Charlottesville, Va, were
available for analysis. Of these, 112 were missing 1 or more of
the cluster variables and were excluded. The features of excluded
children did not differ from those of the final sample (see this
article’s Table E6 in the Online Repository at www.jacionline.
org). The final sample included 161 children. Features of the sam-
ple are presented in Table I. Whereas treatment with combination
ICS and long-acting b-agonist (LABA) therapy was prevalent
even among children with mild-to-moderate asthma (Table I),
the study sample is representative of children with difficult
asthma treated at academic medical centers.
Cluster analysis
Using the agglomerative cluster approach, a dendogram was
generated and revealed 4 clusters of children with shared
phenotypic characteristics (Fig E1). The presence of 4 clusters
was confirmed when the cluster analysis was repeated with alter-
native linkage methods, including the average between groups
and centroid linkage. These clusters were distinguished by
age, race, asthma onset and duration, a history of sinusitis and
gastroesophageal reflux, the degree of atopic sensitization, and
exhaled nitric oxide (Table II). Clusters also differed according
to medication and healthcare use (Table III) and lung function
(Table IV). These lung function differences between clusters
persisted even after stratification by age of enrollment (see this
article’s Tables E7 and E8 in the Online Repository at www.
jacionline.org).
Cluster 1
Forty-eight children were grouped into cluster 1 (termed ‘‘late-
onset symptomatic asthma’’). This cluster had the lowest preva-
lence of severe asthma defined by ATS criteria (n 5 15; 31%) and
GINA or NAEPP criteria (n 5 1; 2%; Fig 1; see this article’s Table
E9 in the Online Repository at www.jacionline.org). Ten (67%) of
the children with ATS-defined severe asthma in this cluster were
hospitalized within the previous year, and 6 (40%) were hospital-
ized for the first time. This cluster was younger with more non-
Hispanic white subjects and was differentiated by an older age
of symptom onset and shorter asthma duration. Although many
children in this cluster had markers of atopy with positive allergy
skin prick tests, the magnitude of allergic sensitization was rela-
tively lesser compared with the other clusters, with lower exhaled
nitric oxide concentrations. Eighty-eight percent (n 5 42) of chil-
dren in this cluster had an asthma exacerbation necessitating a
physician encounter, and 23% (n 5 11) were hospitalized.
Despite having bronchial hyperresponsiveness to methacholine,
these children had relatively normal lung function (or mild airflow
limitation) with minimal hyperinflation (air trapping) and de-
creased airway resistance. Children in cluster 1 were treated
with relatively fewer controller medications including a signifi-
cantly lower daily dose of ICS. Although 21% of this cluster
did report daily short-acting bronchodilator use, this finding
may be related in part to prophylactic treatment of exercise-
induced symptoms. Approximately 69% (n 5 33) of the children
in this group reported that sports were a primary trigger of asthma
symptoms.
Cluster 2
Fifty-two children were assigned to cluster 2 (termed ‘‘early-
onset atopic asthma with normal lung function’’). Whereas 61%
(n 5 28) of children in this cluster had ATS-defined severe
asthma, only 4% (n 5 2) had severe asthma by GINA or NAEPP
criteria (Fig 1). Children were similar in age and race to cluster
1 but had an earlier age of asthma onset, a longer duration of
TABLE I. Features of the sample
Feature
Mild-to-moderate
asthma
n 5 72
Severe
asthma
n 5 89
P
value
Age (y) 11 6 3 11 6 3 .879
Male 40 (56) 49 (55) .571
White 38 (53) 24 (27) .001
Black 27 (38) 56 (63)
Other
Emergency department visit
(previous year)
22 (31) 64 (72) <.001
Hospitalization (previous year) 6 (8) 49 (55) <.001
History of intubation (ever) 2 (3) 22 (25) .002
Parental history of asthma 41 (58) 62 (70) .022
History of atopic dermatitis 35 (49) 54 (61) .114
History of pneumonia 30 (42) 57 (64) .001
History of sinusitis 26 (31) 35 (39) .255
History of gastroesophageal
reflux
8 (11) 31 (35) .001
Daily ICS dose
(mg fluticasone equivalent per
day)
227 6 211 893 6 225 <.001
No ICS 18 (25) 0 <.001
Montelukast 38 (53) 88 (99) <.001
ICS 1 LABA 31 (43) 77 (87) <.001
Daily short-acting
bronchodilators
17 (24) 54 (61) <.001
Daily oral corticosteroids 0 13 (15) <.001
Number of aeroallergen skin
prick responses (out of 12),
median (range)*
1 (0-9) 4 (0-12) <.001
Serum IgE (kU/L), median
(range)*
142 (2-3484) 344 (3-5458) <.001
Blood eosinophils (%), median
(range)*
3.9 (0.3-23.8) 4.4 (0.1-23.6) .684
Baseline FEV1 (% predicted) 94 6 14 85 6 21 .002
Best FEV1 (% predicted) 104 6 14 98 619 .021
Methacholine (PC20), median
(range)*
2.1 (0.1-24.3) 0.9 (0.1-23.1) .047
Severe asthma was defined according to ATS criteria.4,14
Data represent mean 6 SD
or frequency (%) unless otherwise specified.
*Data were logarithmically transformed before analysis.
J ALLERGY CLIN IMMUNOL
FEBRUARY 2011
384 FITZPATRICK ET AL
4. asthma symptoms, and increased markers of atopy, although ex-
haled nitric oxide was not significantly different from cluster 1.
Health care use was again prominent; 88% (n 5 46) of children
in this cluster had a physician encounter for an acute asthma ex-
acerbation within the previous year, and 33% (n 5 17) were hos-
pitalized. Although children in this group were treated more
frequently with controller medications as well as higher daily
doses of ICS, lung function, including spirometric and lung vol-
ume variables, and best postbronchodilator responses were simi-
lar to those observed in cluster 1. However, 52% (n 5 27) reported
daily short-acting bronchodilator use. Because 37% (n 5 19) of
children in this group also reported asthma symptoms with daily
activities such as walking up stairs, it is unlikely that short-acting
bronchodilator use was solely a result of prophylactic therapy
before exercise.
Cluster 3
Thirty-two children were grouped into cluster 3 (termed
‘‘early-onset atopic asthma with mild airflow limitation and
comorbidities’’). Similar to cluster 2, 63% (n 5 12) had ATS-
defined severe asthma, whereas only 16% (n 5 5) had severe
asthma by GINA or NAEPP criteria (Fig 1). This cluster included
fewer non-Hispanic white subjects with an earlier onset of asthma
symptoms and the longest asthma duration. Children in cluster 3
also had elevated exhaled nitric oxide concentrations compared
with clusters 1 and 2 and significant comorbidities, including a
higher prevalence of gastroesophageal reflux and chronic sinusitis
requiring antibiotic treatment. Children in this cluster were also
more likely to be treated with oral corticosteroids. Seventy-two
percent (n 5 23) had a physician encounter for an asthma exacer-
bation within the previous year, and 41% (n 5 13) were hospital-
ized. This cluster was further differentiated by the degree of
airflow limitation and hyperinflation. Although children in cluster
3 had an enhanced bronchodilator response, airflow limitation
was not completely reversed after 6 to 8 inhalations of albuterol.
Children in this cluster also had a lower total lung capacity, in-
creased airway resistance, and greater bronchial hyperresponsive-
ness to methacholine. More than half of this group (n 5 18; 56%)
used short-acting bronchodilators on a daily basis, and 47% (n 5
15) reported asthma symptoms with daily activities such as walk-
ing and climbing stairs.
Cluster 4
Twenty-nine children were assigned to cluster 4 (termed
‘‘early-onset atopic asthma with advanced airflow limitation’’).
Eighty-six percent (n 5 24) of children in this cluster were
classified as having severe asthma according to ATS criteria,
whereas only 14% (n 5 4) met GINA or NAEPP criteria for
severe asthma (Fig 1). Cluster 4 included the highest prevalence
of black subjects and was similar to cluster 3 with regard to
TABLE II. Demographic and atopic features of subjects
Feature
Total
sample
(n 5 161)
Cluster 1
Late-onset symptomatic
asthma with normal
lung function
(n 5 48)
Cluster 2
Early-onset atopic
asthma with normal
lung function
(n 5 52)
Cluster 3
Early-onset atopic
asthma with mild
airflow limitation
(n 5 32)
Cluster 4
Early-onset atopic
asthma with advanced
airflow limitation
(n 5 29)
P
value*
Age (y) 11 6 3 9 6 3 10 6 2 15 6 2 12 6 2 <.001
Male 89 (55) 22 (46) 27 (52) 21 (66) 19 (66) .205
White 62 (39) 26 (54) 25 (48) 8 (25) 3 (10) <.001
Black 83 (52) 15 (31) 25 (48) 19 (59) 24 (83)
Other 14 (9) 7 (15) 2 (4) 5 (16) 2 (7)
Age of asthma diagnosis
(mo)
38 6 39 73 6 46 30 6 29 14 6 12 19 6 17 <.001
Duration of asthma (mo) 99 6 51 38 6 23 95 6 15 170 6 15 129 6 13 <.001
Body mass index >90th
percentile
47 (29) 13 (27) 16 (31) 12 (38) 6 (21) .522
Parental history of asthma 103 (64) 29 (60) 33 (64) 19 (59) 22 (76) .398
History of atopic
dermatitis
89 (55) 24 (50) 29 (56) 15 (47) 21 (72) .179
History of pneumonia 87 (54) 23 (48) 27 (52) 22 (69) 15 (52) .299
History of sinusitis 61 (38) 16 (33) 14 (27) 21 (66) 10 (35) .003
History of
gastroesophageal reflux
39 (24) 7 (15) 13 (25) 11 (34) 8 (28) .028
Number of skin prick
responses (out of 12),
median (range)
3 (0-12) 1 (0-12) 3 (0-12) 4 (0-10) 3 (0-8) .007
Serum IgE (kU/L),
median (range)
548 (2-5458) 105 (2-3484) 405 (3-3511) 216 (25-5458) 361 (7-1800) .005
Blood eosinophils (%),
median (range)
4.1 (0.1-23.8) 2.9 (0.4-13.2) 5.5 (0.4-23.8) 3.9 (0.2-13.9) 5.4 (0.1-23.6) .053
Exhaled nitric oxide
Offline (ppb, n 5 80) 9 (2-46) 7 (2-30) 9 (4-31) 12 (4-27) 14 (7-46) .021
Online (ppb, n 5 81) 20 (3-260) 12 (3-63) 16 (4-74) 21 (6-260) 30 (4-169) .041
Data represent mean 6 SD or frequency (%) unless otherwise specified.
*P value from ANOVA or x2
analysis between the 4 clusters.
Data were logarithmically transformed before analysis.
J ALLERGY CLIN IMMUNOL
VOLUME 127, NUMBER 2
FITZPATRICK ET AL 385
5. asthma onset and asthma duration, although there were fewer co-
morbidities. This cluster was further differentiated by the highest
exhaled nitric oxide values and the highest extent of health care
use. Ninety-seven percent (n 5 28) of children in this group
saw a physician for an acute exacerbation within the previous
year, and 48% (n 5 22) were hospitalized, with 28% (n 5 8) re-
quiring intensive care. Children in cluster 4 were therefore treated
with the highest daily doses of ICS, and most were receiving at
least 3 asthma controller medications. This cluster was also differ-
entiated by the lowest lung function, including baseline airflow
limitation and hyperinflation that were not completely reversed
with bronchodilator administration. Similar to cluster 3, children
in this cluster also had increased airway resistance and greater
bronchial responsiveness to methacholine. Lower total lung ca-
pacity was also observed in this cluster, although this finding
was restricted to children 12 to 17 years of age (Tables E7 and
E8). Daily symptoms requiring short-acting bronchodilator treat-
ment were also common in this group (n 5 16; 55%), and nearly
one half (n 5 14; 48%) reported asthma symptoms with activities
of daily living.
Predictors of cluster assignment
Asthma duration (P < .001), the number of asthma controller
medications (P 5 .001), and baseline FEV1 percent predicted
values (P < .001) were identified as the strongest predictors of
cluster assignment in this sample (Wilks l 5 0.071; x2
5
401.99; P <.001; see this article’s Table E10 in the Online Repos-
itory at www.jacionline.org). These 3 variables alone resulted in
correct classification of 93% of the original subjects (Fig 2) and
92% of cross-validated grouped cases (see this article’s Table
E11 in the Online Repository at www.jacionline.org).
DISCUSSION
Asthma in children is a complicated and heterogeneous disor-
der with distinct phenotypes. By using an unsupervised cluster
analysis in children with a wide range of asthma severity
characterized in the SARP network, we have identified 4 clusters
of childhood asthma with shared phenotypic features. Similar to
the previous SARP report that described increased allergic
sensitization in clusters of adults with early-onset asthma,21,27
clusters of childhood asthma were all atopic, although the magni-
tude of allergic sensitization differed between groups. Asthma du-
ration, the number of asthma controller medications, and baseline
lung function were also major determinants of asthma phenotype
in this cluster analysis. Although children with ATS-defined se-
vere asthma were present in all clusters, no single cluster corre-
sponded well to the definitions of asthma severity proposed in
published guidelines.3,9
This is likely a result of overly stringent
lung function requirements (ie, FEV1 < 60%) for childhood severe
asthma,12
which were extrapolated from adult reference norms.3,9
These findings highlight the complexity and unique differences of
childhood asthma and emphasize the need for unbiased ap-
proaches to refine current guidelines for asthma diagnosis and
treatment in children.
In a previous cluster analysis of adults enrolled in SARP,
Moore et al19
observed 5 distinct clusters of asthma that differed
TABLE III. Medication use and health care use
Variable
Total
sample
(n 5 161)
Cluster 1
Late-onset
symptomatic
asthma with
normal
lung function
(n 5 48)
Cluster 2
Early-onset atopic
asthma with normal
lung function
(n 5 52)
Cluster 3
Early-onset atopic
asthma with mild
airflow limitation
(n 5 32)
Cluster 4
Early-onset atopic
asthma with
advanced airflow
limitation
(n 5 29) P value*
No ICS 17 (11) 11 (23) 1 (2) 5 (16) 0 <.001
Low-dose to moderate-dose ICS 54 (34) 21 (44) 20 (38) 7 (22) 5 (17)
High-dose ICS 90 (56) 16 (33) 31 (59) 20 (63) 24 (83)
Daily ICS dose (mg fluticasone)* 587 6 393 399 6 332 622 6 354 623 6 450 829 6 364 <.001
Daily b-agonist use 77 (44) 10 (21) 27 (52) 18 (56) 16 (55) .002
Controller medications
No controller medications 14 (9) 9 (19) 2 (4) 3 (9) 0 .015
Montelukast only 6 (4) 2 (4) 0 4 (13) 0 .018
ICS only 13 (8) 6 (13) 4 (8) 1 (3) 2 (7) .496
ICS + LABA or montelukast 31 (19) 16 (33) 9 (17) 3 (9) 3 (10) .021
ICS + LABA + montelukast 97 (60) 15 (31) 37 (71) 21 (66) 24 (83) <.001
Omalizumab 3 (2) 0 2 (4) 0 1 (3) .386
Oral corticosteroids 12 (7) 0 4 (8) 5 (16) 3 (10) .062
At least 1 oral corticosteroid burst 120 (75) 31 (65) 41 (79) 23 (72) 26 (90) .128
No. of oral corticosteroid bursts 2 6 3 2 6 2 3 6 3 4 6 4 3 6 2 .018
Health care use (previous year)
None 22 (14) 6 (13) 6 (12) 9 (28) 1 (3) .037
Physician visit for acute symptoms 149 (93) 42 (88) 46 (88) 23 (72) 28 (97) .037
Emergency department visit 87 (54) 20 (42) 32 (62) 18 (56) 17 (59) .217
Hospital admission 55 (3) 11 (23) 17 (33) 13 (41) 14 (48) .116
ICU admission 33 (21) 8 (17) 10 (19) 7 (22) 8 (28) .702
Intubation (ever) 19 (15) 0 9 (21) 4 (20) 6 (24) .018
ICU, Intensive care unit.
Data represent mean 6 SD or frequency (%).
*P value from x2
analysis between the 4 clusters.
Data are mutually exclusive (subjects were ranked by the most severe level of health care use).
J ALLERGY CLIN IMMUNOL
FEBRUARY 2011
386 FITZPATRICK ET AL
6. primarily in the age of asthma onset, allergic sensitization, base-
line lung function, bronchodilator reversibility, medication use,
and health care use. Two of these clusters were associated with
early-onset atopic asthma and normal or relatively mild airflow
obstruction, whereas 2 others were associated with airflow ob-
struction that displayed different degrees of bronchodilator re-
versibility.19
By using a similar characterization method, we
have identified 4 similar clusters of asthma in children, although
the degree of lung function impairment was significantly lesser.
Whereas baseline FEV1 percent predicted values were 75% to
84% in clusters 3 and 4, clusters of adults with early-onset atopic
asthma had baseline FEV1 percent predicted values of 43% to
57%.19
Similarly, the magnitude of FEV1 bronchodilator admin-
istration was significantly greater in children and suggests that
‘‘fixed’’ airflow limitation is not a distinguishing feature of severe
asthma in this age group. Interestingly, children in clusters 3 and 4
did have evidence of hyperinflation (air trapping) both at baseline
and after bronchodilator administration, but to a much lesser ex-
tent than what has been previously reported in adults.19,21
Although the stability of airflow obstruction and hyperinflation
in childhood asthma is not entirely clear, there is increasing
evidence that an important subgroup of children with persistent
wheezing and asthma symptoms acquires significant baseline
airflow limitation by the early adult years.28-30
In the Melbourne
birth cohort study,31
children with severe asthma at 10 years of
age had the lowest FEV1 and FEV1/forced vital capacity ratios
throughout the first 42 years of life.31
Thus the magnitude of air-
flow limitation in childhood asthma may represent an important
marker of progressive asthma that worsens and results in more
severe disease in adults over time. Even in children with mild-
to-moderate asthma, approximately 30% have declines in the
postbronchodilator FEV1 percent predicted value of more than
1% per year regardless of treatment with ICS.32
This observation
may be related to impaired lung growth,33
which could result in
accelerated lung function decline in the adult years. Further study
is needed to understand how lung function changes and evolves in
these clusters with age.
Unlike previous cluster analyses of asthma in adults,18-20
health
care use was not a robust discriminator of cluster assignment in
children. Although children in cluster 4 had the highest degree
of health care use, the majority of children in each cluster had
physician contact for an asthma exacerbation within the previous
year. Although this observation may be an artifact of the study
sample because children in SARP were recruited from academic
medical centers, this finding is also consistent with the episodic
nature of childhood asthma. Indeed, there is an important distinc-
tion between the severity of exacerbations and overall asthma
control.10,34
Whereas asthma severity refers to the required level
of therapy during active treatment of asthma symptoms (ie, the
magnitude of disease activity), asthma control refers to the extent
to which asthma symptoms are alleviated by treatment.35
Although asthma control often predicts the risk of future exacer-
bations,36
children can have severe exacerbations despite limited
symptoms and normal lung function before the event.37
These
children are difficult to evaluate because many are not sympto-
matic between exacerbations and medications may be
TABLE IV. Lung function variables
Variable
Total sample
(n 5 161)
Cluster 1
Late-onset symptomatic
asthma with normal
lung function
(n 5 48)
Cluster 2
Early-onset atopic
asthma with normal
lung function
(n 5 52)
Cluster 3
Early-onset atopic
asthma with mild
airflow limitation
(n 5 32)
Cluster 4
Early-onset atopic
asthma with advanced
airflow limitation
(n 5 29)
P
value*
Baseline spirometry
FVC (% predicted) 99 6 14 102 6 15 101 6 11 93 6 18 92 6 12 .002
FEV1 (% predicted) 89 6 19 96 6 19 91 6 15 84 6 21 75 6 16 <.001
FEV1/FVC 0.78 6 0.11 0.82 6 0.11 0.79 6 0.09 0.72 6 0.10 0.73 6 0.10 <.001
Postbronchodilator
spirometry
FVC (% predicted) 105 6 16 109 6 16 105 6 13 100 6 20 99 6 17 .038
FEV1 (% predicted) 101 6 17 109 6 19 103 6 13 97 6 19 90 6 12 <.001
FEV1/FVC 0.84 6 0.08 0.86 6 0.08 0.86 6 0.06 0.82 6 0.08 0.79 6 0.11 .003
Change in % predicted
FEV1
15 6 16 13 6 15 14 6 14 18 6 19 20 6 19 .220
Baseline lung volumes
TLC (% predicted) 99 6 13 102 6 13 100 6 11 92 6 11 95 6 16 .034
RV (% predicted) 127 6 49 122 6 49 126 6 42 122 6 53 139 6 58 .618
RV/TLC 0.28 6 0.11 0.26 6 0.08 0.26 6 0.08 0.29 6 0.15 0.34 6 0.15 .025
Raw (% predicted) 132 6 68 108 6 46 120 6 63 185 6 68 154 6 84 <.001
Postbronchodilator lung
volumes
TLC (% predicted) 98 6 12 99 6 10 102 6 11 91 6 9 94 6 14 .004
RV (% predicted) 116 6 39 115 6 31 116 6 46 115 6 49 116 6 34 .998
RV/TLC 0.25 6 0.08 0.26 6 0.07 0.24 6 0.08 0.27 6 0.14 0.26 6 0.07 .613
Raw (% predicted) 83 6 33 74 6 36 79 6 36 99 6 41 83 6 33 .170
Methacholine PC20 (mg),
median (range)
1.32
(0.16-23.14)
1.20
(0.09-3.05)
1.13
(0.12-3.02)
0.43
(0.06-3.18)
0.63
(0.25-2.21)
.018
FVC, Forced vital capacity; Raw, airway resistance; RV, residual volume; TLC, total lung capacity.
Data represent mean 6 SD or frequency (%) unless otherwise specified.
*P value from analysis of variance between the 4 clusters.
Data were logarithmically transformed before analysis.
J ALLERGY CLIN IMMUNOL
VOLUME 127, NUMBER 2
FITZPATRICK ET AL 387
7. discontinued. Future revision of definitions of asthma severity
may need to take this observation into account, because the inten-
sity of treatment in these children may not be the best indicator of
impairment and future risk.
An important strength of this study is that cluster analysis, by
definition, is unsupervised, and thus the identified clusters con-
form to shared phenotypic features and not a priori severity as-
signments. This study nonetheless does have limitations. First,
it is unclear whether children enrolled in SARP differ systemati-
cally from children who refused participation. Although selection
bias is a concern in all observational studies, this bias may influ-
ence the conclusions drawn and the generalization of our results,
particularly because the SARP sample was enriched for children
with difficult asthma who are evaluated at academic medical cen-
ters. However, the clinical characteristics associated with asthma
severity in this sample, including lung function measures,
markers of allergic sensitization, and exhaled nitric oxide values,
are similar to what has been previously reported in other samples
of children with severe asthma.5,6,12
Regardless, our sample may
not accurately identify different phenotypes of milder asthma se-
verity that are likely encountered in clinical practice. Thus, ex-
pansion of our study to children with more mild intermittent
forms of asthma would likely have resulted in additional subjects
and therefore subclustering within clusters 1 and 2. Second, al-
though enrollment of additional non-Hispanic white subjects
would have led to a more geographically representative sample,
the disproportionate grouping of black subjects in clusters 3 and
4 likely reflects important ethnic differences in asthma pheno-
types. Because health care use was highly prevalent in each clus-
ter, the disproportionate racial distributions are not solely
attributable to health care access. Indeed, other genetic-based
studies have shown that black subjects with asthma have the ear-
liest age of asthma onset, the strongest family history of asthma,
and the lowest baseline FEV1 percent predicted values compared
with white and Hispanic subjects.38
Third, it is also important to
note that the results obtained from cluster analysis may be depen-
dent on the cluster technique used. Because a cluster analysis will
always find patterns in data, regardless of the organization of the
dataset, there is not a single best method for performing the anal-
ysis. Thus, the inclusion of more children would likely have re-
sulted in further subclustering within our 4 identified clusters.
For this reason, these results must be interpreted within the larger
clinical context. Although all children in this study were stable at
the time of assessment, the stability of these clusters over time and
in response to different or novel asthma interventions (including
pharmacologic therapies) is unknown. Thus, the predictive as-
pects of these clusters are also unclear and will require validation
in future longitudinal studies of childhood asthma. A separate val-
idation in a different and perhaps larger sample of children with
severe asthma would also be useful to understand better the het-
erogeneity of the disorder.
In conclusion, we have identified 4 clusters of childhood
asthma in the NIH/NHLBI SARP. Foremost, these data empha-
size that asthma, particularly severe asthma, is a highly hetero-
geneous disorder. Importantly, no identified cluster corresponded
entirely to definitions of severe asthma proposed by national and
international guidelines or the ATS. Although this may reflect our
variable selection, the consensus-based definitions of severe
asthma may also require further validation in children. Whereas
the GINA and NAEPP criteria for severe asthma are based
primarily on symptoms and lung function, our pediatric asthma
clusters were determined as much by the magnitude of atopy and
duration of asthma as by airflow limitation and hyperinflation.
Exhaled nitric oxide concentrations and the age of asthma
symptom onset were also differentiating features of the clusters,
whereas health care use was a lesser determinant. These data
highlight the complexity and heterogeneity of childhood asthma
FIG 1. A, Frequency of children with mild, moderate, and severe asthma de-
fined by NAEPP or GINA guidelines. B, Frequency of children with mild-to-
moderate and severe asthma defined by ATS criteria in each cluster (cluster
1, black bars; cluster 2, white bars; cluster 3, gray bars; cluster 4, hatched
bars).
FIG 2. Scatterplot of the discriminant functions generated from discrimi-
nant analysis of asthma duration, the extent of asthma controller therapy,
and baseline FEV1 percent predicted values. Each data point represents a
single subject. The plot depicts clustering and separation of cluster 1 (white
triangles), cluster 2 (gray circles), cluster 3 (black squares), and cluster 4
(white diamonds) using these 3 variables.
J ALLERGY CLIN IMMUNOL
FEBRUARY 2011
388 FITZPATRICK ET AL
8. and support the need for additional studies, including validation
of these clusters in other samples of children with severe asthma.
If these clusters are indeed clinically meaningful, then cluster
analysis and other unsupervised approaches may ultimately assist
with the refinement of current guidelines for asthma diagnosis and
treatment in children.
The SARP is a multicenter asthma research group funded by the NHLBI and
consisting of the following contributors (principal investigators are marked
with an asterisk): Brigham & Women’s Hospital, Elliot Israel,* Bruce D.
Levy, Michael E. Wechsler, Shamsah Kazani, Gautham Marigowda; Cleve-
land Clinic, Serpil C. Erzurum,* Raed A. Dweik, Suzy A. A. Comhair, Emmea
Cleggett-Mattox, Deepa George, Marcelle Baaklini, Daniel Laskowski;
Emory University, Anne M. Fitzpatrick, Denise Whitlock, Shanae Wakefield;
Imperial College School of Medicine, Kian Fan Chung,* Mark Hew, Patricia
Macedo, Sally Meah, Florence Chow; University of Iowa, Eric Hoffman,*
Janice Cook-Granroth; University of Pittsburgh, Sally E. Wenzel,* Fernando
Holguin, Silvana Balzar, Jen Chamberlin; University of Texas—Medical
Branch, William J. Calhoun,* Bill T. Ameredes; University of Virginia, Ben-
jamin Gaston,* W. Gerald Teague,* Denise Thompson-Batt; University of
Wisconsin, William W. Busse,* Nizar Jarjour, Ronald Sorkness, Sean Fain,
Gina Crisafi; Wake Forest University, Eugene R. Bleecker,* Deborah Meyers,
Wendy Moore, Stephen Peters, Rodolfo M. Pascual, Annette Hastie, Gregory
Hawkins, Jeffrey Krings, Regina Smith; Washington University in St Louis,
Mario Castro,* Leonard Bacharier, Jaime Tarsi; Data Coordinating Center,
Douglas Curran-Everett,* Ruthie Knowles, Maura Robinson, Lori Silveira;
NHLBI, Patricia Noel, Robert Smith.
Clinical implications: Cluster analysis identifies distinct pheno-
types of asthma in children that do not correspond to definitions
of asthma severity proposed by current guidelines. Clusters of
asthma in adults can also be indentified in children, but with im-
portant differences.
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3. Expert Panel Report 3 (EPR-3): guidelines for the diagnosis and management of
asthma-summary report 2007. J Allergy Clin Immunol 2007;120:S94-138.
4. Fitzpatrick AM, Gaston BM, Erzurum SC, Teague WG. Features of severe asthma
in school-age children: atopy and increased exhaled nitric oxide. J Allergy Clin Im-
munol 2006;118:1218-25.
5. Bossley CJ, Saglani S, Kavanagh C, Payne DN, Wilson N, Tsartsali L, et al. Cor-
ticosteroid responsiveness and clinical characteristics in childhood difficult asthma.
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6. Chipps BE, Szefler SJ, Simons FE, Haselkorn T, Mink DR, Deniz Y, et al. Demo-
graphic and clinical characteristics of children and adolescents with severe or
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related with severity: a 1-yr prospective study. Eur Respir J 2002;19:61-7.
8. Sullivan SD, Rasouliyan L, Russo PA, Kamath T, Chipps BE. Extent, patterns, and
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62:126-33.
9. Bateman ED, Hurd SS, Barnes PJ, Bousquet J, Drazen JM, FitzGerald M, et al.
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asthma. Proc Am Thorac Soc 2009;6:712-9.
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second the best measure of severity in childhood asthma? Am J Respir Crit Care
Med 2004;169:784-6.
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sifying asthma severity in children: mismatch between symptoms, medication use,
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14. Lang AM, Konradsen J, Carlsen KH, Sachs-Olsen C, Mowinckel P, Hedlin G, et al.
Identifying problematic severe asthma in the individual child—does lung function
matter? Acta Paediatr 2010;99:404-10.
15. Proceedings of the ATS workshop on refractory asthma: current understanding,
recommendations, and unanswered questions. American Thoracic Society. Am J
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17. Von Mutius E. Presentation of new GINA guidelines for paediatrics. The Global
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An official American Thoracic Society/European Respiratory Society statement:
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Overall asthma control: the relationship between current control and future risk.
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9. METHODS
Cluster analysis was performed with SAS version 9.1 (SAS Institute Inc,
Cary, NC). The Ward minimum-variance hierarchical clustering method was
performed by using an agglomerative (bottom-up) approach and Ward
linkage. At each generation of clusters, samples were merged into larger
clusters to minimize and maximize with within-subjects and between-subjects
sum of squares, respectively. ANOVAwith Tukey post hoc testing and x2
tests
were used to determine differences between groups. To determine the stron-
gest predictors of cluster assignment, stepwise discriminant analysis of the
12 cluster variables was performed with the Fisher method, which is robust
against departures from normality. This method yields a set of discriminant
functions on the basis of the linear combinations of variables that provide
the best discrimination between groups. Previous probabilities for group as-
signment were adjusted for the number of cases included in the analysis. Co-
variance of the predictor variables was assessed by using pooled within-groups
matrices and Box M tests. The ability of the canonical discriminant functions
to distinguish between groups was further evaluated by Wilks l and x2
tests.
All variables were entered simultaneously using the Wilks l method. Entry
and removal probabilities for the F statistic were set at 0.05 and 0.10, respec-
tively. Cross-validation was performed by classifying each case by the func-
tions derived from all other cases.
REFERENCES
E1. Proceedings of the ATS workshop on refractory asthma: current understanding,
recommendations, and unanswered questions. American Thoracic Society. Am
J Respir Crit Care Med 2000;162:2341-51.
E2. Bateman ED, Hurd SS, Barnes PJ, Bousquet J, Drazen JM, FitzGerald M, et al.
Global strategy for asthma management and prevention: GINA executive sum-
mary. Eur Respir J 2008;31:143-78.
E3. Expert Panel Report 3 (EPR-3): guidelines for the diagnosis and management of
asthma-summary report 2007. J Allergy Clin Immunol 2007;120(suppl 5):
S94-138.
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389.e1 FITZPATRICK ET AL
10. FIG E1. Dendogram. Using the Wald minimum-variance hierarchical clustering method and an agglom-
erative (bottom-up approach), 161 subjects from the NIH/NHLBI SARP were clustered into a single final
group. At each generation of clusters, samples were merged into larger clusters to minimize the within-
cluster sum of squares or maximize the between-subjects sum of squares. With successive clustering, 4
groups were apparent.
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11. TABLE E1. The NIH/NHLBI SARP definition of severe asthma
Major criteria for severe asthma (must have at least 1 to achieve asthma control):
d Treatment with high-dose ICSs
d Treatment with continuous oral corticosteroids (at least 50% of the year)
Minor criteria for severe asthma (must have at least 2):
d Treatment with additional controller medications to maintain asthma control
d Daily use of short-acting bronchodilators (5 of 7 days)
d Persistent airflow obstruction, with baseline FEV1 <80% predicted
d One or more urgent care visits for asthma in the previous year
d Three or more oral corticosteroid bursts in the previous year
d A history of prompt deterioration in asthma symptoms with a reduction in the dose of ICS or oral corticosteroids
d A near-fatal asthma event requiring intubation in the past
The SARP definition of severe asthma was adopted from the ATS’s Workshop on Refractory Asthma.E1
According to this definition, for subjects to have severe asthma, they must
have at least 1 (of 2) major criteria and at least 2 (of 7) minor criteria. This definition further assumes that subjects are adherent with their prescribed asthma therapy and that all
relevant comorbidities have been addressed and treated accordingly.
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13. TABLE E3. Variables included in the cluster analysis
Variable Variable type Variable name Key
1 Continuous Asthma duration In months
2 Continuous Baseline FEV1 % predicted before bronchodilation
3 Continuous Maximum FEV1 % predicted after maximal bronchodilation
4 Categoric Race White, black, other
5 Categoric Sex Male, female
6 Categoric ICS group None, low-dose ICS, high-dose ICS
7 Semiquantitative b-Agonist use 0: Never
1: Once per month
2: Weekly
3: Daily
8 Composite Asthma controller medications 0: None
1: Montelukast or ICS monotherapy
2: ICS plus montelukast or LABA
3: ICS plus montelukast and LABA
4: Oral corticosteroids or omalizumab
9 Composite Health care use in the previous year 0: None
1: Emergency visit for asthma (physician/emergency department)
2: >_3 oral corticosteroid bursts
3: Hospital admission
4: Intensive care unit admission
10 Semiquantitative Frequency of symptoms 0: Once a month or less
1: Weekly, but less than twice per week
2: Weekly, but less than once per day
3: Daily
11 Semiquantitative Atopic sensitization 0: Ln IgE <5.53, <_2 positive skin tests
1: Ln IgE >5.53, <_2 positive skin tests
2: Ln IgE <5.53, >_3 positive skin tests
3: Ln IgE >5.53, >_3 positive skin tests
12 Semiquantitative Exhaled nitric oxide quartile 0: Lowest (offline <6.4, online <6.7 ppb)
1: First (offline <9.1, online <18.1 ppb)
2: Second (offline <14.5, online <38 ppb)
3: Highest (offline >14.5, online >38 ppb)
Ln, Natural logarithm.
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14. TABLE E4. Generation and performance of the composite variable atopic sensitization
Atopic sensitization composite variable responses n High-dose ICS (%)
Hospitalized
previous year (%)
Baseline
FEV1 (%)
Asthma duration
(mo)
Ln IgE <5.53, <_2 positive skin tests 57 39 26 98.34 79.86
Ln IgE >5.53, <_2 positive skin tests 24 71 46 89.27 100.83
Ln IgE <5.53, >_3 positive skin tests 34 66 41 92.10 109.71
Ln IgE >5.53, >_3 positive skin tests 59 74 41 85.93 111.39
Ln, Natural logarithm.
This composite variable was generated from a median split of serum IgE (ln IgE cut point of 5.53, which corresponds to a serum IgE of 252 kU/L) data and a median split of the
number of positive skin prick responses (cut point of 2). The 5 columns on the right verify that the variable effectively discriminates between ICS dose, health care use, lung
function, and duration of asthma.
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15. TABLE E5. Generation and performance of the composite variable exhaled nitric oxide quartile
Exhaled nitric oxide composite variable responses n High-dose ICS (%)
Hospitalized
previous year (%)
Baseline
FEV1 (%) Log eosinophils Log IgE
Lowest (offline <6.4, online <6.7 ppb) 36 41 22 94.44 0.40 4.40
First (offline <9.1, online <18.1 ppb) 42 51 26 95.91 0.51 5.00
Second (offline <14.5, online <38 ppb) 38 67 47 88.39 0.62 5.75
Highest (offline >14.5, online >38 ppb) 51 65 41 86.09 0.66 6.08
Because exhaled nitric oxide was measured in some children by using offline methods and in others by using online methods, quartiles of exhaled nitric oxide were assigned to
offline values and online values separately. Cut points for offline exhaled nitric oxide quartiles were 6.4 ppb (25th percentile), 9.1 ppb (50th percentile), and 14.5 ppb (75th
percentile). Cut points for online exhaled nitric oxide quartiles were 6.7 ppb (25th percentile), 18.1 ppb (50th percentile), and 38 ppb (75th percentile). The separate quartile
assignments were then merged to create 1 variable. The 4 columns on the right verify that the variable effectively discriminates between ICS dose, health care use, lung function,
blood eosinophils, and IgE.
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16. TABLE E6. Features of children excluded from cluster analysis
because of missing data
Feature
Excluded
children
n 5 112
Included
children
n 5 161
P
value
Age (y) 12 6 2 11 6 3 .762
Male/female 60/40 55/45 .464
White/black/other 30/60/10 39/52/9 .422
ATS-defined severe asthma 67 55 .431
Asthma duration (mo) 106 6 25 99 6 50 .607
Controller medications
ICS only 7 8 .661
ICS 1 LABA or montelukast 20 19 .584
ICS 1 LABA 1 montelukast 67 60 .423
Baseline FEV1 (% predicted) 94 6 13 89 6 19 .221
Data represent mean 6 SD or percentage.
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19. TABLE E9. GINA and NAEPP criteria for severe persistent asthmaE2,E3
Feature of severe asthma GINA guidelines NAEPP guidelines (children 5-11 y) NAEPP guidelines (children >_12 y)
Daytime asthma Symptoms Daily Throughout the day Throughout the day
Nocturnal asthma symptoms Frequent Often (7 times/wk) Often (7 times/wk)
Exacerbations Frequent >_2 times/y >_2 times/y
Activities Limited Extremely limited Extremely limited
FEV1 <60% predicted FEV1
variability >30%
<60% predicted FEV1/FVC <75% <60% predicted FEV1/FVC
reduced >5%
FVC, Forced vital capacity.
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20. TABLE E10. Results of stepwise linear discriminant analysis of the 12 variables used in cluster analysis
Step Variables included Tolerance Significance of F to remove Wilks l F value P value
1 Asthma duration 1.000 403.03 0.115 403.03 <.001
2 Asthma duration 0.958 389.74 0.103 110.01 <.001
No. of controller medications 0.958 6.01
3 Asthma duration 0.935 372.76 0.089 71.59 <.001
No. of controller medications 0.832 8.67
Baseline FEV1% predicted 0.864 8.423
Asthma duration, the number of asthma controller medications, and baseline FEV1 percent predicted values were identified as the strongest predictors of cluster assignment.
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21. TABLE E11. Cluster assignment classification results according to asthma duration, the number of asthma controller medications, and
baseline FEV1 percent predicted values
Predicted cluster membership
Model Cluster Cluster 1 Cluster 2 Cluster 3 Cluster 4 Total
Original model Cluster 1 43 5 0 0 48
Cluster 2 0 50 0 2 52
Cluster 3 0 0 31 1 32
Cluster 4 0 1 2 26 29
Cross-validated model* Cluster 1 43 5 0 0 48
Cluster 2 0 50 0 2 52
Cluster 3 0 0 30 2 32
Cluster 4 0 1 3 25 29
Data were generated from stepwise linear discriminant analysis and represent the number of subjects.
*Cross-validation was performed by classifying each case by the functions derived from all other cases (excluding the case of interest).
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