Mise-en-scene refers to everything within the frame of a shot that can be used to enhance the audience's understanding, including costumes, makeup, setting, props, and lighting. It aims to create a realistic feeling and help viewers comprehend the emotions and roles of characters. Key elements of mise-en-scene are costumes, which convey a character's identity; makeup, which can set the time period and distinguish characters; setting, which provides context for understanding roles; and props and lighting, which further immerse the audience in the story and its themes. Together, these components allow filmmakers to interact with and express meaning to audiences visually as well as verbally.