This document describes Ant Colony Optimization, an algorithm inspired by ant behavior that can be applied to routing protocols in wired and wireless networks. It discusses how biological ants are able to find the shortest path between their nest and a food source by leaving pheromone trails. The algorithm is implemented in Java and demonstrated on sample networks, showing convergence on optimal paths. Examples are given of how it has been adapted to routing protocols and been shown to outperform protocols like OSPF and RIP in terms of efficiency and scalability. Results of tests on different networks found that ant-based routing algorithms delivered packets with less overhead compared to protocols like DSR and AODV.