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Answer "True or False" to the following statements Colony is formed from a large number of
bacterial cells. Both spread-plate and pour plate methods will only yield pure culture.
Turbidometeric methods count only live cells. Increase in turbidity is an index of bacterial
growth and biomass. The primary stain of the gram's staining reaction is called safranin. The
secondary/counter stain of the grams staining reaction is methylene blue. Gram positive cells
have an extra layer in the cell component. Carbolfuchsin is used in staining acid fast organisms.
Nucleic acids usually bind to basic dyes. Blood agar medium is an enrichment medium instead
of a selective medium. A clone consists different kinds of bacteria. A pure culture can be
formed from streaked plate method.
Solution
Group of bacteria grow on solid media called as colony. Hence colony formed by large number
of bacterial cells. True statement. Spread plate method gives the pure culture. Pour plating dose
not gives the pure culture , used for quantitative urine cultures.meny methods are there for
isolation of pure culture example, enrichment media, selective media, indicator media,
pretreatment, inoculation etc. Hence false statement. Turbidometry counts both live and dead
cells. Hence false statement. True statement, increased turbidity index for bacterial growth and
biomass. False, safranine is used for counter staining ,but not for primary staining. False,
methylene blue is used for primary staining not for secondary staining. False statement, cell wall
of positive bacteria has peptidoglycon layer and techoic acid. Gram negative has,
lipopolysaccharides, outer membrane, lipoproteins and peptidoglycon. True statement,
carbolfuchsin used for staining acid fast organisms. True statement, nucleic acids bind to basic
dyes. True statement, blood agar is not selective media. False statement, clone consis of similar
bacteria. True statement, streak culture or surface plating is used in pure culture.

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  • 1. Answer "True or False" to the following statements Colony is formed from a large number of bacterial cells. Both spread-plate and pour plate methods will only yield pure culture. Turbidometeric methods count only live cells. Increase in turbidity is an index of bacterial growth and biomass. The primary stain of the gram's staining reaction is called safranin. The secondary/counter stain of the grams staining reaction is methylene blue. Gram positive cells have an extra layer in the cell component. Carbolfuchsin is used in staining acid fast organisms. Nucleic acids usually bind to basic dyes. Blood agar medium is an enrichment medium instead of a selective medium. A clone consists different kinds of bacteria. A pure culture can be formed from streaked plate method. Solution Group of bacteria grow on solid media called as colony. Hence colony formed by large number of bacterial cells. True statement. Spread plate method gives the pure culture. Pour plating dose not gives the pure culture , used for quantitative urine cultures.meny methods are there for isolation of pure culture example, enrichment media, selective media, indicator media, pretreatment, inoculation etc. Hence false statement. Turbidometry counts both live and dead cells. Hence false statement. True statement, increased turbidity index for bacterial growth and biomass. False, safranine is used for counter staining ,but not for primary staining. False, methylene blue is used for primary staining not for secondary staining. False statement, cell wall of positive bacteria has peptidoglycon layer and techoic acid. Gram negative has, lipopolysaccharides, outer membrane, lipoproteins and peptidoglycon. True statement, carbolfuchsin used for staining acid fast organisms. True statement, nucleic acids bind to basic dyes. True statement, blood agar is not selective media. False statement, clone consis of similar bacteria. True statement, streak culture or surface plating is used in pure culture.