This document discusses genetics research on Alzheimer's disease. It covers several key points:
- Three genes - PSEN1, PSEN2, and APP - are responsible for early-onset Alzheimer's disease in rare familial cases. PSEN1 mutations account for the most cases.
- ApoE4 is the strongest genetic risk factor for the more common late-onset Alzheimer's disease. Carriage of one or two ApoE4 alleles increases risk and decreases amyloid clearance.
- Genome-wide association studies have identified new genetic loci associated with Alzheimer's risk, including Clusterin and PICALM, though Alzheimer's has a complex genetic architecture.
- Ongoing research aims to discover biomarkers, through
Genome Wide Association Studies in PsychiatryDr.Guru S Gowda
Neuropsychiatric and neurodevelopmental disorders are multifactorial in origin with multiple genetic determinants and its interaction with others resulting in clinically diverse phenotypes. Affected individuals harbour different risk alleles in a heterogeneous genetic background that make candidate disorder genes difficult to detect via studies. One such effort to find the influence of genetic factors is Genome Wide Association Study (GWAS), which is an approach to identify common genetic variations like Single-Nucleotide Polymorphism (SNP) and Copy Number Variation (CNV) associated with a disease / traits. It is a powerful method for such investigations, particularly by enabling the integrated analysis of SNPs and CNVs. There are now hundreds of candidate genes with DNA copy number variations or single nucleotide polymorphisms (SNPs) characterised from clinically diagnosed individuals.
With more mature molecular and statistical methods, we are entering a new era of Network and Pathway Analyses. This may reveal coherent biological processes contributing to psychiatric illnesses but the evidence is limited to date.
This seminar aims at providing a broad overview on GWAS - History, approach to G W A S, GWAS studies in Psychiatry and its Implication, complex network and pathway analysis concepts, current challenges etc, and ends with conclusion and future directions.
Circulating Biomarkers for Alzheimer's Disease: Neurodegenerative Disorders ...QIAGEN
Alzheimer's disease (AD) is a complex neurodegenerative disorder. Circulating miRNAs hold great promise in the discovery of non-invasive and novel biomarkers for AD diagnosis and prognosis. This slideshow presents the role of miRNAs in AD and details current progress in biomarker discovery. Various tools for pathway-focused and genome-wide miRNA expression profiling, miRNA functional studies and target identification are also included.
Genome Wide Association Studies in PsychiatryDr.Guru S Gowda
Neuropsychiatric and neurodevelopmental disorders are multifactorial in origin with multiple genetic determinants and its interaction with others resulting in clinically diverse phenotypes. Affected individuals harbour different risk alleles in a heterogeneous genetic background that make candidate disorder genes difficult to detect via studies. One such effort to find the influence of genetic factors is Genome Wide Association Study (GWAS), which is an approach to identify common genetic variations like Single-Nucleotide Polymorphism (SNP) and Copy Number Variation (CNV) associated with a disease / traits. It is a powerful method for such investigations, particularly by enabling the integrated analysis of SNPs and CNVs. There are now hundreds of candidate genes with DNA copy number variations or single nucleotide polymorphisms (SNPs) characterised from clinically diagnosed individuals.
With more mature molecular and statistical methods, we are entering a new era of Network and Pathway Analyses. This may reveal coherent biological processes contributing to psychiatric illnesses but the evidence is limited to date.
This seminar aims at providing a broad overview on GWAS - History, approach to G W A S, GWAS studies in Psychiatry and its Implication, complex network and pathway analysis concepts, current challenges etc, and ends with conclusion and future directions.
Circulating Biomarkers for Alzheimer's Disease: Neurodegenerative Disorders ...QIAGEN
Alzheimer's disease (AD) is a complex neurodegenerative disorder. Circulating miRNAs hold great promise in the discovery of non-invasive and novel biomarkers for AD diagnosis and prognosis. This slideshow presents the role of miRNAs in AD and details current progress in biomarker discovery. Various tools for pathway-focused and genome-wide miRNA expression profiling, miRNA functional studies and target identification are also included.
Genome-wide association study (GWAS) technology has been a primary method for identifying the genes responsible for diseases and other traits for the past ten years. GWAS continues to be highly relevant as a scientific method. Over 2,000 human GWAS reports now appear in scientific journals. Our free eBook aims to explain the basic steps and concepts to complete a GWAS experiment.
"Epigenetics refers to genetic factors that change an organism’s appearance or biological functions without changing the actual DNA sequence. In other words, gene expression changes but the genes themselves don’t. Epigenetics adds an additional level of complexity to the genetic code." - Public Health Cafe
Synopsis
Introduction
Some Facts
Types of SNPs
SNPs act as gene markers
Methods of Detection
Techniques to detect SNPs
Allelic Specific Cleavage
Differential Hybridization
Single Base Extension or minisequencing
Alternate Methods for Detecting SNPs
Mass Spectrometry
Microchips
SIGNIFICANCE OF SNPs
HAPLOTYPE
ADVANTAGES
Are SNP data available to the public?
Some important SNP database Resources
CONCLUSION
References
-Basic Concepts in Genetics
-What is Epigenetic?
-History of Epigenetic
-How do epigenetics work?
-Epigenetics and the Environment
-Epigenetic Inheritance
-Epigenetics in Psychiatry
Single Nucleotide Polymorphism Analysis
Predictive Analytics and Data Science Conference May 27-28
Asst. Prof. Vitara Pungpapong, Ph.D.
Department of Statistics
Faculty of Commerce and Accountancy
Chulalongkorn University
Epigenetics is the study of heritable changes in gene expression (active versus inactive genes) that do not involve changes to the underlying DNA sequence — a change in phenotype without a change in genotype — which in turn affects how cells read the genes. - [https://www.whatisepigenetics.com/fundamentals/]
Author of this presentation: The University of Western Australia
Genome-wide association study (GWAS) technology has been a primary method for identifying the genes responsible for diseases and other traits for the past ten years. GWAS continues to be highly relevant as a scientific method. Over 2,000 human GWAS reports now appear in scientific journals. Our free eBook aims to explain the basic steps and concepts to complete a GWAS experiment.
"Epigenetics refers to genetic factors that change an organism’s appearance or biological functions without changing the actual DNA sequence. In other words, gene expression changes but the genes themselves don’t. Epigenetics adds an additional level of complexity to the genetic code." - Public Health Cafe
Synopsis
Introduction
Some Facts
Types of SNPs
SNPs act as gene markers
Methods of Detection
Techniques to detect SNPs
Allelic Specific Cleavage
Differential Hybridization
Single Base Extension or minisequencing
Alternate Methods for Detecting SNPs
Mass Spectrometry
Microchips
SIGNIFICANCE OF SNPs
HAPLOTYPE
ADVANTAGES
Are SNP data available to the public?
Some important SNP database Resources
CONCLUSION
References
-Basic Concepts in Genetics
-What is Epigenetic?
-History of Epigenetic
-How do epigenetics work?
-Epigenetics and the Environment
-Epigenetic Inheritance
-Epigenetics in Psychiatry
Single Nucleotide Polymorphism Analysis
Predictive Analytics and Data Science Conference May 27-28
Asst. Prof. Vitara Pungpapong, Ph.D.
Department of Statistics
Faculty of Commerce and Accountancy
Chulalongkorn University
Epigenetics is the study of heritable changes in gene expression (active versus inactive genes) that do not involve changes to the underlying DNA sequence — a change in phenotype without a change in genotype — which in turn affects how cells read the genes. - [https://www.whatisepigenetics.com/fundamentals/]
Author of this presentation: The University of Western Australia
"Impact of front-end architecture on development cost", Viktor TurskyiFwdays
I have heard many times that architecture is not important for the front-end. Also, many times I have seen how developers implement features on the front-end just following the standard rules for a framework and think that this is enough to successfully launch the project, and then the project fails. How to prevent this and what approach to choose? I have launched dozens of complex projects and during the talk we will analyze which approaches have worked for me and which have not.
Slack (or Teams) Automation for Bonterra Impact Management (fka Social Soluti...Jeffrey Haguewood
Sidekick Solutions uses Bonterra Impact Management (fka Social Solutions Apricot) and automation solutions to integrate data for business workflows.
We believe integration and automation are essential to user experience and the promise of efficient work through technology. Automation is the critical ingredient to realizing that full vision. We develop integration products and services for Bonterra Case Management software to support the deployment of automations for a variety of use cases.
This video focuses on the notifications, alerts, and approval requests using Slack for Bonterra Impact Management. The solutions covered in this webinar can also be deployed for Microsoft Teams.
Interested in deploying notification automations for Bonterra Impact Management? Contact us at sales@sidekicksolutionsllc.com to discuss next steps.
Software Delivery At the Speed of AI: Inflectra Invests In AI-Powered QualityInflectra
In this insightful webinar, Inflectra explores how artificial intelligence (AI) is transforming software development and testing. Discover how AI-powered tools are revolutionizing every stage of the software development lifecycle (SDLC), from design and prototyping to testing, deployment, and monitoring.
Learn about:
• The Future of Testing: How AI is shifting testing towards verification, analysis, and higher-level skills, while reducing repetitive tasks.
• Test Automation: How AI-powered test case generation, optimization, and self-healing tests are making testing more efficient and effective.
• Visual Testing: Explore the emerging capabilities of AI in visual testing and how it's set to revolutionize UI verification.
• Inflectra's AI Solutions: See demonstrations of Inflectra's cutting-edge AI tools like the ChatGPT plugin and Azure Open AI platform, designed to streamline your testing process.
Whether you're a developer, tester, or QA professional, this webinar will give you valuable insights into how AI is shaping the future of software delivery.
JMeter webinar - integration with InfluxDB and GrafanaRTTS
Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
Essentials of Automations: Optimizing FME Workflows with ParametersSafe Software
Are you looking to streamline your workflows and boost your projects’ efficiency? Do you find yourself searching for ways to add flexibility and control over your FME workflows? If so, you’re in the right place.
Join us for an insightful dive into the world of FME parameters, a critical element in optimizing workflow efficiency. This webinar marks the beginning of our three-part “Essentials of Automation” series. This first webinar is designed to equip you with the knowledge and skills to utilize parameters effectively: enhancing the flexibility, maintainability, and user control of your FME projects.
Here’s what you’ll gain:
- Essentials of FME Parameters: Understand the pivotal role of parameters, including Reader/Writer, Transformer, User, and FME Flow categories. Discover how they are the key to unlocking automation and optimization within your workflows.
- Practical Applications in FME Form: Delve into key user parameter types including choice, connections, and file URLs. Allow users to control how a workflow runs, making your workflows more reusable. Learn to import values and deliver the best user experience for your workflows while enhancing accuracy.
- Optimization Strategies in FME Flow: Explore the creation and strategic deployment of parameters in FME Flow, including the use of deployment and geometry parameters, to maximize workflow efficiency.
- Pro Tips for Success: Gain insights on parameterizing connections and leveraging new features like Conditional Visibility for clarity and simplicity.
We’ll wrap up with a glimpse into future webinars, followed by a Q&A session to address your specific questions surrounding this topic.
Don’t miss this opportunity to elevate your FME expertise and drive your projects to new heights of efficiency.
Connector Corner: Automate dynamic content and events by pushing a buttonDianaGray10
Here is something new! In our next Connector Corner webinar, we will demonstrate how you can use a single workflow to:
Create a campaign using Mailchimp with merge tags/fields
Send an interactive Slack channel message (using buttons)
Have the message received by managers and peers along with a test email for review
But there’s more:
In a second workflow supporting the same use case, you’ll see:
Your campaign sent to target colleagues for approval
If the “Approve” button is clicked, a Jira/Zendesk ticket is created for the marketing design team
But—if the “Reject” button is pushed, colleagues will be alerted via Slack message
Join us to learn more about this new, human-in-the-loop capability, brought to you by Integration Service connectors.
And...
Speakers:
Akshay Agnihotri, Product Manager
Charlie Greenberg, Host
Accelerate your Kubernetes clusters with Varnish CachingThijs Feryn
A presentation about the usage and availability of Varnish on Kubernetes. This talk explores the capabilities of Varnish caching and shows how to use the Varnish Helm chart to deploy it to Kubernetes.
This presentation was delivered at K8SUG Singapore. See https://feryn.eu/presentations/accelerate-your-kubernetes-clusters-with-varnish-caching-k8sug-singapore-28-2024 for more details.
Smart TV Buyer Insights Survey 2024 by 91mobiles.pdf91mobiles
91mobiles recently conducted a Smart TV Buyer Insights Survey in which we asked over 3,000 respondents about the TV they own, aspects they look at on a new TV, and their TV buying preferences.
Builder.ai Founder Sachin Dev Duggal's Strategic Approach to Create an Innova...Ramesh Iyer
In today's fast-changing business world, Companies that adapt and embrace new ideas often need help to keep up with the competition. However, fostering a culture of innovation takes much work. It takes vision, leadership and willingness to take risks in the right proportion. Sachin Dev Duggal, co-founder of Builder.ai, has perfected the art of this balance, creating a company culture where creativity and growth are nurtured at each stage.
Key Trends Shaping the Future of Infrastructure.pdfCheryl Hung
Keynote at DIGIT West Expo, Glasgow on 29 May 2024.
Cheryl Hung, ochery.com
Sr Director, Infrastructure Ecosystem, Arm.
The key trends across hardware, cloud and open-source; exploring how these areas are likely to mature and develop over the short and long-term, and then considering how organisations can position themselves to adapt and thrive.
6. Proteomics: Data-mining
McCUSKER
RESEARCHFOUNDATION
INC
ALZHEIMER’S
DIAN is a 6 year multicentre study involving 10 sites worldwide.
The major aim of the study is:
AIM:
To collecting and analysing clinical and neuropsychological sequence of
changes that occurring in dominantly inherited AD by neuroimaging
(MRI, FTD-PET and PIB-PET) and neuropathological testing.
REQUIREMENT:
The study is seeking to establish a cohort of 400 people
who are at high risk of developing autosomal dominant AD.
The cost of research is US$10,000 per person every 3 years.
STUDY LAYOUT:
The study will commence in the second half of 2010 and
Each site (of which the Sir James McCusker ADRU is one)
will aim to recruit 40 people. Participants will undergo
periodic assessments over 6 years, including clinical and
neuropsychological assessment, collection of blood and
CSF, and neuroimaging.
EARLY DIAGNOSIS & BIOMARKERS
7. Proteomics: Data-mining
McCUSKER
RESEARCHFOUNDATION
INC
ALZHEIMER’S
ALZHEIMER DISEASE: GENETICS
PSEN1
PSEN2
APP
Other
EARLY ONSET AD (EOAD)
Age at onset < 65 years
≤5% of AD cases
Familial (EOFAD)
3 Gene Loci
Autosomal Dominant Inheritance
LATE ONSET AD (LOAD)
Age at onset > 65 years
~95% of AD cases
Familial
Predominantly Sporadic
Multiple Gene Loci
- Genetic Risk Factors
9. Proteomics: Data-mining
McCUSKER
RESEARCHFOUNDATION
INC
ALZHEIMER’S
APOLIPOPROTEIN E: GENETIC RISK
*Martins et al., 1995, NeuroReport;
Laws et al., 1999, NeuroReport
0 5 10 15
1/2
2
Odds Ratio*
vs. 0: 3.7
vs. 1: 6.2
vs. 0/1: 12.7
vs. 0: 18.8
#ofε4Alleles
Zhong, Weisgraber, 2009,
J. Biol. Chem.
Estimated human genotype
frequency of APOE
Allele ε2 ε3 ε4
ε2 ~1% ~11% ~2%
ε3 ~62% ~22%
ε4 ~2%
AD: ~43% ~15%
All ethnicities
http://www.alzgene.org
10. Proteomics: Data-mining
McCUSKER
RESEARCHFOUNDATION
INC
ALZHEIMER’S
APOLIPOPROTEIN E: GENETIC RISK
Reiman et al., 2009
Proc Natl Acad Sci U S A., 106(16): 6820–6825
Peripheral Clearance of Beta Amyloid
following tail vein injection of 20 μg Aβ42
n=5
E3
n=5
E4
n=5
E2
5 min 10 min 15 min 30 min 60 min 90 min
Aβ Clearance: ApoE knock-in Mice
(8 Wks Old)
non-E4E4
13. Proteomics: Data-mining
McCUSKER
RESEARCHFOUNDATION
INC
ALZHEIMER’S
BIOMARKER DISCOVERY AND THE IDENTIFICATION OF A DIAGNOSTIC SIGNATURE
AIM: Identify the best biomarker (a substance used as an indicator of a biological state e.g.
Alzheimer’s) signature for the diagnosis, prediction and monitoring of Alzheimer’s disease.
SIGNIFICANCE:
• Early diagnosis or accurate prediction
prevention/treatment strategies initiated
when they are most effective
• Monitoring response to medical/lifestyle
interventions.
FUTURE WORK:
• Discovery of the optimal panel of biomarkers
is likely to require a combined approach.
Proteins, Lipids and Genes (‘multi-omic’)
• Best chance of capturing the optimal panel
of biomarkers.
EARLY DIAGNOSIS & BIOMARKERS
Editor's Notes
Novel Genetic FactorsCandidate Gene ApproachLinkage StudiesGenome Wide Association Studies