Agrobacteirum use in Gene Transfer Techniques

1. DISCOVER . LEARN . EMPOWER
Agrobacterium mediated gene transfer
UNIVERSITY INSTITUTE OF
BIOTECHNOLOGY
Bechlor’s of Science
Subject Name: Genetic Engineering
Subject Code: BTT-231

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32. imitations of cloning with Agrobacterium plasmids
Higher plants are divided into two broad categories, the monocots and the dicots.
Several factors have combined to make it much easier to clone genes in dicots such as
tomato, tobacco, potato, peas, and beans, but much more difficult to obtain the same
results with monocots. This has been frustrating because monocots include wheat,
barley, rice, and maize, which are the most important crop plants and hence the most
desirable targets for genetic engineering projects.
imitations of cloning with Agrobacterium plasmids
Higher plants are divided into two broad categories, the monocots and the dicots.
Several factors have combined to make it much easier to clone genes in dicots such as
tomato, tobacco, potato, peas, and beans, but much more difficult to obtain the same
results with monocots. This has been frustrating because monocots include wheat,
barley, rice, and maize, which are the most important crop plants and hence the most
desirable targets for genetic engineering projects.
monocots were totally resistant to transformation with Ti and Ri vectors

33. Attempts to circumvent this problem have centered on the use of biolistics—
bombardment with microprojectiles (p. 85)—to introduce plasmid DNA directly into
plant embryos. Although this is a fairly violent transformation procedure it does not
appear to be too damaging for the embryos, which still continue their normal development program to produce
mature plants. The approach has been successful with maize
and several other important monocots

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