This study evaluated retinal parameters in 37 healthy young adults using OCT to determine normal inter-ocular asymmetry values. Statistically significant differences were found between the left and right eyes in mean and superior retinal nerve fiber layer thickness and central macular thickness, with larger values in the left eye. Inter-ocular asymmetry tolerance limits were also obtained. Significant differences in mean retinal nerve fiber layer thickness were also found between males and females in the right eye. These findings provide support for exploring normal retinal asymmetries as an approach for early glaucoma detection. However, differences in instrumentation and samples limit direct comparisons to other research.