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Information must be
provided
In pathology request form
and other forms
Prepared:
By:Dr.AhmadReshadWajed
18/12/2017
arwajed.com
Request Form: Document bearing patient
information, nature of sample collected and the
details of testing required. This may be in either
paper or in electronic (e.g. ICE) format.
arwajed.com
INFORMATION MUST BE PROVIDED
IN PATHOLOGY REQUEST FORM
 Patient Identification
 Name:
 F/Name:
 Age:
 Sex:
 Phone Number:
 Medical Record Number:
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 Current Address:
1-Province
2-District
3-lenght of stay
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 Tissue Identification /site of biopsy
 labeling
 Orientation
 Fixative
 Chief Complaint
 Current Medications
 Previous Treatment /Operative procedure
 Clinical Diagnosis
 Requested
 Date &Time of Operation
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 Doctor Identification
 Name of Doctor
 Email Address
 Doctor Phone Number
 Signature
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This section to be filled by Pathology Lab
 Submitted by
 Phone #:
 Signature:
 Received by:
 Date&Time
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PATIENT NAME
Why patient name is necessary
 For cancer registry
 For different between multiple same name
 For prevent the mistake
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 The patient's details should not be written in the
form or on the container by pencil
 It should be write by pen
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PATIENT FATHER NAME
 Why patient father name is necessary
 It define the personality of a person
 Make different between same names maybe many
patients have same name
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PATIENT AGE
 Why patient age is important
 Oldest in high level face with cancer then young
 Youngest less face with cancer
 If we find a case of cancer with youngest it is
cancelable it need more attention.
 Molecular diagnostics is a collection of techniques
used to analyse biological markers in the
genome done with other member of family
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WHY SEX OF PATIENT IS IMPORTANT
 there is a great deal to be said about the
incidence of cancers in different sexes. Some
cases, like general malignancy, female face more
then male with breast cancer. breast cancers are
not common with males. Gender is important. We
have three genera in general, male, female, and
(third generation) between male and female
 important for consolidating the majority of cases
 Lung cancer
 Colorectal cancer bath type of cancer are more
common in male
 Breast cancer is more common in female and also
 Colon cancer
 Endometrial cancer
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PHONE#
 It is a way to connect with when somethings not
clear in pathology request form belong to the
patient.
 If we have some advice for patient by phone
connect with him or her and give the advice
 When some time tell about dosage of drug for
patient you can tell by phone
 When the patient life away form hospital
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MEDICAL RECORD NUMBER
follow-up of patients in cancer registry
When the doctor wants see the the information
contained in the medical record allows health care
providers to determine the patient's medical
history and provide informed care. The medical
record serves as the central repository for planning
patient care and documenting communication
among patient and health care provider and
professionals contributing to the patient's care. An
increasing purpose of the medical record is to
ensure documentation of compliance with
institutional, professional or governmental
regulation.
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LOCATION
 Location is important?
 In location………..
 -Province
 -District
 In length of stay
 Is importin
 Goiter cancer must commune in some place in
Afghanistan like parwan province in the
mountainous areas and also commune in some
part of nangarhar province, where Iodine
deficiency is seen, people are more likely to
develop Goiter cancer, depending on the areas
where the iodine surface is normal
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LABELING NUMBER WITH
 the specimen are accessioned by giving them a
number with labeling that will identify each
specimen for each patient
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 Specimen •
 MRN number OR other unique identity number
• Patient surname • Patient first name • Date of
birth • Initialled / Signed by specimen collector •
Date of specimen collection
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 Samples must never be pre labelled, labelling
should be on the body of the container and never
on the lid.
 All samples MUST be clearly labelled in the
presence of the patient.
 Details in bold are essential, labels will be
accepted but hand written samples are preferable
 Last name/Surname (or coded identifier as in GU
patients); • First name; • Date of Birth; •
NHS/Hospital number; • Date and time of sample;
• (and signature) Initials of person who took
sample Source/location of patient;
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 Equipment Pathology request forms Patient
identification labels Specimen containers e.g.
blood tubes, urine pots Specimen collection
Biohazard bags Sealed buckets
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SITE OF BIOPSY
 Why site biopsy is important Because if the
location of the sample is not clear, the pathologist
face with problem drying diagnosis and also some
of the symptoms that are the same, such as the
neck and cervix, it should be clear that the site of
the sample, as the sample is taken from stomach
, should be determined from which part of the
stomach is taken from
the,duodenum,cardia,body,small intestines,
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ORIENTATION
Orientation of the specimen becomes important when the
margins of the wound must be proven to be free of tumor
The tumor edges are marked with suture or other method
The STSR should accurately communicate the significance
of the markers to the circulator or pathologist
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ORIENTATION
 A--------
 B--------
 C--------
 D--------
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CLINICAL DIAGNOSIS
 This section is also very important. It shows the
doctor's attention, which is how much sick it is. It
shows the doctor's study
 It is very important that you contact your doctor
if you need to. Sometimes some of the issues are
dumb in the form of postponement or
postponement of the patient's chronic
complication
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FIXATIVE
 Complex series of chemical events to preserve the
basic structure of the tissue in such a manner
that subsequent examination may be made to
determine the microanatomy and allow
localization of its various chemical constituents

 The purpose of fixation is to preserve tissues
permanently in life-like
 state as possible
arwajed.com
 Formalin 10%
 90% water 10% formalin best fixative for all
kind of biopsy
 Formaldehyde37%

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CHIEF COMPLAINT
 Why should the main complaints come from?
Why should the main complaints come from?
This section is also important because it is
difficult for a patient to suffer for a long time, but
he has not been able to refer to the pathology
center,
 Face with some problem like..
 Family
 Traditional
 Religious
 Lack of knowledge
 Poverty
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 The date of the operation is important The date
of the operation is important It is important to
know when the operation is performed and when
the biopsy is delivered to its center, the sample
should be sent to the center after the operation if
there is an unnecessary delay, it may cause
autolysis or spontaneous destruction, or cause
tissue necrosis, which is irreversible. The sample
should be transmitted immediately to its center,
otherwise it loses its importance.
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CURRENT MEDICATIONS
It is important before the patient pathology
diagnosis or after the pathology diagnosis if the
patient take drug tell about that make clear it
 Chimotrapy
 Antibiotic
 Specially tell about chemo the patient take the
chemo or not take chemo
relevant medication e.g. drug therapy, coagulation
related medication
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PREVIOUS TREATMENT /OPERATIVE
PROCEDURE
 If the patient take any kind of drug before take
complete history about it form patient
 If the patient before do any operation also clear it

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CLINICAL DIAGNOSIS
 This section clear first idea of doctor before the
histopathologic diagnosis
 Doctor know about the anatomy and histology of
a organ after the operation finish the doctor can
say it is maybe cancer .
 When the biopsy form breast can estimated it is
invasive ductal carcinoma
 Fibro adenoma
 Fibrocystic change
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REQUESTED
 When you send the biopsy for pathology
examination tell in pathology request form for
pathology center you send the simple for which
kind of examination
 H&E
 Specially exam
 HER2--------0-3 from 0-+1Nig +2 +3 ---+3
positive +2 rep
 1-FISH/ Flrocent insitu hybridisation
 2-CISH/ Chromogenic in situ hybridisation
 ICH/ Immunohistochemistry
 Molecular exam

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DATE &TIME OF OPERATION
 Make clear date and time of operation it is
necessary when the operation is done on which
time
 Date:02/05/2017
 Time: 2:30pm:
 After the operation the biopsy send to pathology
deportment for diagnosis in fixative biopsy will
be received on time not late if it is received late
without any fixative lost the diagnosis value
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DOCTOR IDENTIFICATION
 Name of doctor
 Signature of doctor
 Telephone Number of doctor
 Email address of doctor
arwajed.com
 Submitted by
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‫اهداف‬‫تثبیت‬
FIXATION GOALS
1-‫جلوگیری‬‫ویا‬ ‫خودبخودی‬ ‫ازتخریب‬‫انساج‬ ‫اتولیز‬
2.‫نموده‬ ‫جلوگیری‬ ‫انتانات‬ ‫ازمداخله‬
3.‫شود‬ ‫اسانی‬ ‫به‬ ‫ان‬ ‫کارروی‬ ‫که‬ ‫نسج‬ ‫نمودن‬ ‫سخت‬ ‫جهت‬
4.‫درنسج‬ ‫نوری‬ ‫تراکم‬ ‫افزایش‬
5.‫نسج‬ ‫شدن‬ ‫ازجمع‬ ‫جلوگیری‬
6.‫تلوین‬ ‫مواد‬ ‫به‬ ‫سازکاری‬
8.‫،رنگ‬ ‫شکل‬ ‫حفظ‬ ‫امکان‬ ‫تاحد‬‫بیوپسی‬ ‫واندازه‬
7.‫میکند‬ ‫جلوگیری‬ ‫درانساج‬ ‫ازمرگ‬ ‫بعد‬ ‫ازتغیرات‬ ‫درکل‬
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‫کننده‬ ‫تثبیت‬ ‫محلول‬
‫شده‬ ‫قبول‬ ‫که‬ ‫کننده‬ ‫تثبیت‬ ‫محلول‬ ‫بهترین‬
‫میباشد‬ ‫فیصد‬ ‫ده‬ ‫فارملین‬ ‫است‬ ‫جهانی‬
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‫فارملین‬10%
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‫فرمالین‬10%
‫بهترین‬ ‫عنوان‬ ‫به‬‫میگردد‬ ‫قرار‬ ‫استفاده‬ ‫مورد‬ ‫ها‬ ‫نمونه‬ ‫دراکثر‬ ‫که‬ ‫است‬ ‫کننده‬ ‫تثبیت‬.
‫محلول‬ ‫غلظت‬ ‫با‬ ‫خالص‬ ‫فرمالین‬40%‫است‬ ‫آب‬ ‫در‬ ‫فرمالدئید‬ ‫گاز‬.
‫فرمالین‬10%‫محلول‬ ‫غلظت‬ ‫با‬4%‫است‬ ‫آب‬ ‫در‬ ‫فرمالین‬ ‫گاز‬.
‫بین‬ ‫رقت‬ ‫با‬ ‫نهایی‬ ‫غلظت‬8-12%‫با‬ ‫ای‬ ‫مالحظه‬ ‫قابل‬ ‫تفاوت‬ ‫و‬ ‫شده‬ ‫پذیرفته‬10%‫ندارد‬.
‫فرمالین‬ ‫تهیه‬ ‫برای‬10%‫مقدار‬ ،‫خالص‬ ‫فرمالین‬ ‫از‬10‫با‬ ‫خالص‬ ‫فرمالین‬ ‫سی‬ ‫سی‬90
‫گردد‬ ‫می‬ ‫مخلوط‬ ‫آب‬ ‫سی‬ ‫سی‬.
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‫فرمالین‬10%
‫حداقل‬ ‫باید‬ ‫فرمالین‬ ‫حجم‬10‫باشد‬ ‫بافت‬ ‫برابر‬.
‫ظ‬‫از‬ ‫بعد‬ ‫که‬ ‫طوری‬ ‫به‬ ،‫باشد‬ ‫داشته‬ ‫بزرگ‬ ‫درب‬ ‫باید‬ ‫نمونه‬ ‫حاوی‬ ‫رف‬
‫شود‬ ‫آورده‬ ‫در‬ ‫ظرف‬ ‫از‬ ‫راحتی‬ ‫به‬ ‫نمونه‬ ،‫فیکساسیون‬.
‫کند‬ ‫احاطه‬ ‫طرف‬ ‫هر‬ ‫از‬ ‫را‬ ‫نمونه‬ ‫باید‬ ‫فرمالین‬.
‫پوشانده‬ ‫گاز‬ ‫وسیله‬ ‫به‬ ‫بایستی‬ ‫هستند‬ ‫فرمالین‬ ‫در‬ ‫شناور‬ ‫که‬ ‫بزرگ‬ ‫های‬ ‫نمونه‬
‫شوند‬.
‫گیرند‬ ‫می‬ ‫قرار‬ ‫ظرف‬ ‫کف‬ ‫در‬ ‫که‬ ،‫بزرگ‬ ،‫ای‬ ‫صفحه‬ ،‫سنگین‬ ‫های‬ ‫نمونه‬ ‫برای‬
‫گیرد‬ ‫قرار‬ ‫نمونه‬ ‫و‬ ‫ظرف‬ ‫کف‬ ‫بین‬ ‫گاز‬ ‫بایستی‬.
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‫فرمالین‬10%
‫ماه‬ ‫در‬ ‫ولی‬ ‫است‬ ‫خوب‬ ‫شود‬ ‫اطاق‬ ‫دردرجه‬ ‫اگر‬ ‫کردن‬ ‫تثبیت‬
‫زمستان‬‫کنیم‬ ‫گرم‬ ‫را‬ ‫فارملین‬ ‫متوانیم‬ ‫را‬ ‫فارملین‬
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‫فواید‬‫فرمالین‬10%
‫ارزان‬
‫ماندن‬ ‫باقی‬‫نمونه‬‫تخریب‬ ‫بدون‬ ،‫طوالنی‬ ‫مدت‬ ‫به‬ ‫آن‬ ‫در‬
‫مانند‬ ‫خاص‬ ‫های‬ ‫آمیزی‬ ‫رنگ‬ ‫اکثریت‬ ‫با‬ ‫آن‬ ‫مطابقت‬IHC
‫شدن‬ ‫چملک‬‫حداقل‬‫نمونها‬
‫حفظ‬DNA‫در‬ ‫فری‬ ‫با‬ ‫فرمالین‬ ‫با‬4‫سانتیگراد‬ ‫درجه‬
‫است‬ ‫دسترس‬ ‫قابل‬ ‫زیاد‬
‫است‬ ‫ارزان‬
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‫دیگر‬ ‫مهم‬ ‫نکات‬....
OTHER IMPORTANT POINTS ....
1.‫بیو‬ ‫نکات‬ ‫درتمام‬ ‫مساویآ‬ ‫بشکل‬ ‫باید‬ ‫فارملین‬‫شود‬ ‫منتشر‬ ‫پسی‬
2-‫شود‬ ‫گذاشته‬ ‫نسج‬ ‫ترازخود‬ ‫کالن‬ ‫کانتینر‬ ‫در‬ ‫باید‬ ‫نسج‬
3-‫باید‬ ‫کننده‬ ‫تثبیت‬ ‫محلول‬6‫تا‬10‫باشد‬ ‫نسج‬ ‫خود‬ ‫برابر‬
4-‫باشد‬ ‫شناور‬ ‫کننده‬ ‫تثبیت‬ ‫محلول‬ ‫بین‬ ‫در‬ ‫کانتینر‬ ‫بین‬ ‫در‬ ‫خوبی‬ ‫به‬ ‫باید‬ ‫نسج‬
5-‫کننده‬ ‫تثبیت‬ ‫محلول‬ ‫بخاطریکه‬ ‫شود‬ ‫شق‬ ‫باید‬ ‫باشد‬ ‫بزرگ‬ ‫نسج‬ ‫که‬ ‫درصورت‬
‫درنسج‬ ‫بتواند‬ ‫کرده‬ ‫نفوذ‬ ‫بخوبی‬
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‫باشد‬ ‫عملیات‬ ‫اطاق‬ ‫نزدیک‬ ‫شفاخانه‬ ‫داخل‬ ‫در‬ ‫تشخیصه‬ ‫مرکز‬ ‫که‬ ‫بود‬ ‫خواهد‬ ‫بهتر‬
‫وبه‬ ‫داده‬ ‫قرار‬ ‫کننده‬ ‫تثبیت‬ ‫درمحلول‬ ‫را‬ ‫ان‬ ‫بیوپسی‬ ‫ازگرفتن‬ ‫پس‬ ‫بالفاصله‬ ‫وتازه‬
‫مناسب‬ ‫ظروف‬ ‫در‬ ‫باید‬ ‫نمونها‬ ‫تشخیص‬ ‫غرض‬ ‫دهیم‬ ‫انتقال‬ ‫پتالوژی‬ ‫بخش‬
‫شود‬ ‫گذاشته‬ ‫ای‬ ‫شیشه‬ ‫یا‬ ‫و‬ ‫پالستیکی‬
‫شود‬ ‫داده‬ ‫انتقال‬ ‫تشخیصه‬ ‫مرکز‬ ‫به‬ ‫نمونها‬ ‫تاخیر‬ ‫کدام‬ ‫بدون‬ ‫که‬ ‫شود‬ ‫کوشش‬
‫تاخیر‬ ‫کدام‬ ‫اگر‬ ‫درصورت‬‫بیو‬ ‫درانتقال‬‫بیوپسی‬ ‫درفاملین‬ ‫باید‬ ‫بدهد‬ ‫رخ‬ ‫پسی‬
‫شود‬ ‫جلوگیری‬ ‫دران‬ ‫ازمرگ‬ ‫بعد‬ ‫تاازتغیرات‬ ‫شود‬ ‫گذاشته‬
‫شود‬ ‫گذاشته‬ ‫یخچال‬ ‫دربین‬ ‫نمونه‬ ‫کننده‬ ‫تثبیت‬ ‫محلول‬ ‫نبودن‬ ‫درصورت‬ ‫ولی‬
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‫شود‬ ‫فرستاده‬ ‫شود‬ ‫انداخته‬ ‫کالن‬ ‫ظرف‬ ‫یک‬ ‫بین‬ ‫در‬ ‫نمونه‬ ‫که‬ ‫شود‬ ‫کوشش‬
‫از‬ ‫نیست‬ ‫شفاخانه‬ ‫داخل‬ ‫در‬ ‫ظرف‬ ‫امکانات‬ ‫اگر‬‫پایوازمریض‬‫خواسته‬
‫بیاورد‬ ‫بیرون‬ ‫از‬ ‫را‬ ‫کالن‬ ‫ظرف‬ ‫یک‬ ‫که‬ ‫شود‬
‫میباشد‬ ‫ذیل‬ ‫نواقص‬ ‫داری‬ ‫شود‬ ‫انداخته‬ ‫نمونه‬ ‫درپالستیک‬ ‫اگر‬
1-‫شود‬ ‫پاره‬ ‫راه‬ ‫در‬ ‫ان‬ ‫انتقان‬ ‫جریان‬ ‫در‬ ‫پالستیک‬ ‫دارد‬ ‫امکان‬
2-‫نور‬ ‫با‬ ‫زود‬ ‫بسیار‬ ‫گیرد‬ ‫قرار‬ ‫افتاب‬ ‫مستقیم‬ ‫شعاع‬ ‫مقابل‬ ‫در‬ ‫پالستیک‬ ‫اگر‬
‫میشود‬ ‫پزیری‬ ‫رنگ‬ ‫پروسه‬ ‫تغیر‬ ‫سبب‬ ‫و‬ ‫میکند‬ ‫تعامل‬ ‫افتاب‬
3-‫داده‬ ‫قرار‬ ‫است‬ ‫دوا‬ ‫ان‬ ‫اثرات‬ ‫دران‬ ‫که‬ ‫دیگر‬ ‫دوا‬ ‫بوتل‬ ‫بین‬ ‫در‬ ‫نباید‬ ‫نمونه‬
‫معقطع‬ ‫تغیر‬ ‫سبب‬ ‫میگرد‬ ‫را‬ ‫مواد‬ ‫همان‬ ‫رنگ‬ ‫نمونه‬ ‫که‬ ‫بخاطریکه‬ ‫شود‬
‫میشود‬
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‫پتالوژی‬
6-‫شود‬ ‫انجام‬ ‫جراح‬ ‫داکتر‬ ‫ازطرف‬ ‫کالن‬ ‫کتله‬ ‫شق‬
‫میکند‬ ‫کمک‬ ‫تشخیص‬ ‫در‬ ‫کی‬ ‫مهم‬ ‫نکات‬ ‫بخاطرحفظ‬
7-‫و‬ ‫شده‬ ‫نسج‬ ‫نکروز‬ ‫سبب‬ ‫تواند‬ ‫نه‬ ‫نفوذکرده‬ ‫نسج‬ ‫دربین‬ ‫اگرمحلول‬ ‫چرا‬
‫میدهد‬ ‫دست‬ ‫از‬ ‫را‬ ‫تشخیصه‬ ‫ارزش‬
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‫میشود‬ ‫گرفته‬ ‫جراح‬ ‫توسط‬ ‫نمونه‬
‫به‬ ‫و‬ ‫داده‬ ‫قرار‬ ‫کننده‬ ‫تثبیت‬ ‫محلول‬ ‫در‬ ‫را‬ ‫ان‬ ‫جراح‬ ‫توسط‬ ‫کتله‬ ‫برداشتن‬ ‫از‬ ‫بعد‬
‫که‬ ‫است‬ ‫داکتر‬ ‫وظیفه‬ ‫تشخیص‬ ‫جهت‬ ‫بالفاصله‬ ‫شود‬ ‫داده‬ ‫انتقال‬ ‫تشخیصه‬ ‫مرکز‬
‫مکمل‬ ‫تاریخچه‬ ‫کند‬ ‫ترتیب‬ ‫راجستر‬ ‫ورق‬ ‫تا‬ ‫داده‬ ‫دستور‬ ‫خود‬ ‫دستان‬ ‫زیر‬ ‫به‬
‫داده‬ ‫قرار‬ ‫کننده‬ ‫تثبیت‬ ‫مناسب‬ ‫محلول‬ ‫در‬ ‫را‬ ‫نمونه‬ ‫نماید‬ ‫درج‬ ‫دران‬ ‫مریض‬
‫وری‬ ‫و‬ ‫ترباشد‬ ‫بزرگ‬ ‫نمونه‬ ‫خود‬ ‫از‬ ‫درکانتینرکه‬ ‫فیصد‬ ‫ده‬ ‫فارملین‬ ‫مانند‬
‫گردد‬ ‫درج‬ ‫مریض‬ ‫به‬ ‫راجع‬ ‫معلومات‬ ‫تمام‬ ‫کانتینر‬
‫مربوطه‬ ‫وارد‬ ‫یا‬ ‫بخش‬ ‫عملیات‬ ‫جستر،تاریخ‬ ‫را‬ ‫،ولد،سن،شماره‬ ‫اسم‬.........
arwajed.com
‫به‬ ‫راجع‬ ‫معلومات‬ ‫تمام‬ ‫باید‬ ‫مریض‬ ‫پتالوژی‬ ‫معاینات‬ ‫درخواستی‬ ‫ورق‬ ‫در‬
‫پری‬ ‫خانه‬ ‫داکتر‬ ‫خود‬ ‫توسط‬ ‫باید‬ ‫مریض‬ ‫درخواستی‬ ‫ورق‬ ‫شود‬ ‫درج‬ ‫مریض‬
‫را‬ ‫مریض‬ ‫چون‬ ‫جراح‬ ‫داکتر‬ ‫بخاطریکه‬ ‫دیگر‬ ‫صحی‬ ‫کارمند‬ ‫کدام‬ ‫توسط‬ ‫نه‬ ‫شود‬
‫میتواند‬ ‫دارد‬ ‫معلومات‬ ‫موارد‬ ‫،وغیره‬ ‫شکل‬ ‫حدود‬ ‫مورد‬ ‫در‬ ‫کرده‬ ‫عملیات‬
‫درست‬ ‫بشکل‬ ‫نماید‬ ‫ارایه‬ ‫را‬ ‫خوبتر‬ ‫معلومات‬
‫اسم،ولد،سن،جنس،محلول‬ ‫گردد‬ ‫درج‬ ‫مریض‬ ‫ورق‬ ‫در‬ ‫باید‬ ‫که‬ ‫معلومات‬
‫و‬ ‫مریض‬ ‫قبلی‬ ‫،عملیات‬ ‫مریض‬ ‫قبلی‬ ‫،تداویی‬ ‫دیگر‬ ‫مریضی‬ ‫نمونه،تاریخچه‬
‫اکر‬ ‫میشود‬ ‫داده‬ ‫انتقال‬ ‫نمونه‬ ‫ان‬ ‫در‬ ‫که‬ ‫محلول‬ ‫وذکر‬ ‫عملیات‬ ‫تاریخ‬ ‫همچنان‬
‫گردد‬ ‫ذکر‬ ‫مریض‬ ‫معاینات‬ ‫درخواستی‬ ‫ورق‬ ‫در‬ ‫باید‬ ‫باشد‬ ‫استخوان‬ ‫نمونه‬
‫است‬ ‫متفاوت‬ ‫کامآل‬ ‫استخوان‬ ‫باال‬ ‫کار‬ ‫شیوه‬ ‫چون‬ ‫است‬ ‫استخوان‬ ‫نمونه‬ ‫که‬
arwajed.com

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About pathology sending biopsy

  • 1. Information must be provided In pathology request form and other forms Prepared: By:Dr.AhmadReshadWajed 18/12/2017 arwajed.com
  • 2. Request Form: Document bearing patient information, nature of sample collected and the details of testing required. This may be in either paper or in electronic (e.g. ICE) format. arwajed.com
  • 3. INFORMATION MUST BE PROVIDED IN PATHOLOGY REQUEST FORM  Patient Identification  Name:  F/Name:  Age:  Sex:  Phone Number:  Medical Record Number: arwajed.com
  • 5.  Tissue Identification /site of biopsy  labeling  Orientation  Fixative  Chief Complaint  Current Medications  Previous Treatment /Operative procedure  Clinical Diagnosis  Requested  Date &Time of Operation arwajed.com
  • 6.  Doctor Identification  Name of Doctor  Email Address  Doctor Phone Number  Signature arwajed.com
  • 7. This section to be filled by Pathology Lab  Submitted by  Phone #:  Signature:  Received by:  Date&Time arwajed.com
  • 8. PATIENT NAME Why patient name is necessary  For cancer registry  For different between multiple same name  For prevent the mistake arwajed.com
  • 9.  The patient's details should not be written in the form or on the container by pencil  It should be write by pen arwajed.com
  • 10. PATIENT FATHER NAME  Why patient father name is necessary  It define the personality of a person  Make different between same names maybe many patients have same name arwajed.com
  • 11. PATIENT AGE  Why patient age is important  Oldest in high level face with cancer then young  Youngest less face with cancer  If we find a case of cancer with youngest it is cancelable it need more attention.  Molecular diagnostics is a collection of techniques used to analyse biological markers in the genome done with other member of family arwajed.com
  • 12. WHY SEX OF PATIENT IS IMPORTANT  there is a great deal to be said about the incidence of cancers in different sexes. Some cases, like general malignancy, female face more then male with breast cancer. breast cancers are not common with males. Gender is important. We have three genera in general, male, female, and (third generation) between male and female  important for consolidating the majority of cases  Lung cancer  Colorectal cancer bath type of cancer are more common in male  Breast cancer is more common in female and also  Colon cancer  Endometrial cancer arwajed.com
  • 13. PHONE#  It is a way to connect with when somethings not clear in pathology request form belong to the patient.  If we have some advice for patient by phone connect with him or her and give the advice  When some time tell about dosage of drug for patient you can tell by phone  When the patient life away form hospital arwajed.com
  • 14. MEDICAL RECORD NUMBER follow-up of patients in cancer registry When the doctor wants see the the information contained in the medical record allows health care providers to determine the patient's medical history and provide informed care. The medical record serves as the central repository for planning patient care and documenting communication among patient and health care provider and professionals contributing to the patient's care. An increasing purpose of the medical record is to ensure documentation of compliance with institutional, professional or governmental regulation. arwajed.com
  • 15. LOCATION  Location is important?  In location………..  -Province  -District  In length of stay  Is importin  Goiter cancer must commune in some place in Afghanistan like parwan province in the mountainous areas and also commune in some part of nangarhar province, where Iodine deficiency is seen, people are more likely to develop Goiter cancer, depending on the areas where the iodine surface is normal arwajed.com
  • 16. LABELING NUMBER WITH  the specimen are accessioned by giving them a number with labeling that will identify each specimen for each patient arwajed.com
  • 17.  Specimen •  MRN number OR other unique identity number • Patient surname • Patient first name • Date of birth • Initialled / Signed by specimen collector • Date of specimen collection arwajed.com
  • 18.  Samples must never be pre labelled, labelling should be on the body of the container and never on the lid.  All samples MUST be clearly labelled in the presence of the patient.  Details in bold are essential, labels will be accepted but hand written samples are preferable  Last name/Surname (or coded identifier as in GU patients); • First name; • Date of Birth; • NHS/Hospital number; • Date and time of sample; • (and signature) Initials of person who took sample Source/location of patient; arwajed.com
  • 19.  Equipment Pathology request forms Patient identification labels Specimen containers e.g. blood tubes, urine pots Specimen collection Biohazard bags Sealed buckets arwajed.com
  • 20. SITE OF BIOPSY  Why site biopsy is important Because if the location of the sample is not clear, the pathologist face with problem drying diagnosis and also some of the symptoms that are the same, such as the neck and cervix, it should be clear that the site of the sample, as the sample is taken from stomach , should be determined from which part of the stomach is taken from the,duodenum,cardia,body,small intestines, arwajed.com
  • 21. ORIENTATION Orientation of the specimen becomes important when the margins of the wound must be proven to be free of tumor The tumor edges are marked with suture or other method The STSR should accurately communicate the significance of the markers to the circulator or pathologist arwajed.com
  • 22. ORIENTATION  A--------  B--------  C--------  D-------- arwajed.com
  • 23. CLINICAL DIAGNOSIS  This section is also very important. It shows the doctor's attention, which is how much sick it is. It shows the doctor's study  It is very important that you contact your doctor if you need to. Sometimes some of the issues are dumb in the form of postponement or postponement of the patient's chronic complication arwajed.com
  • 24. FIXATIVE  Complex series of chemical events to preserve the basic structure of the tissue in such a manner that subsequent examination may be made to determine the microanatomy and allow localization of its various chemical constituents   The purpose of fixation is to preserve tissues permanently in life-like  state as possible arwajed.com
  • 25.  Formalin 10%  90% water 10% formalin best fixative for all kind of biopsy  Formaldehyde37%  arwajed.com
  • 26. CHIEF COMPLAINT  Why should the main complaints come from? Why should the main complaints come from? This section is also important because it is difficult for a patient to suffer for a long time, but he has not been able to refer to the pathology center,  Face with some problem like..  Family  Traditional  Religious  Lack of knowledge  Poverty arwajed.com
  • 27.  The date of the operation is important The date of the operation is important It is important to know when the operation is performed and when the biopsy is delivered to its center, the sample should be sent to the center after the operation if there is an unnecessary delay, it may cause autolysis or spontaneous destruction, or cause tissue necrosis, which is irreversible. The sample should be transmitted immediately to its center, otherwise it loses its importance. arwajed.com
  • 28. CURRENT MEDICATIONS It is important before the patient pathology diagnosis or after the pathology diagnosis if the patient take drug tell about that make clear it  Chimotrapy  Antibiotic  Specially tell about chemo the patient take the chemo or not take chemo relevant medication e.g. drug therapy, coagulation related medication arwajed.com
  • 29. PREVIOUS TREATMENT /OPERATIVE PROCEDURE  If the patient take any kind of drug before take complete history about it form patient  If the patient before do any operation also clear it  arwajed.com
  • 30. CLINICAL DIAGNOSIS  This section clear first idea of doctor before the histopathologic diagnosis  Doctor know about the anatomy and histology of a organ after the operation finish the doctor can say it is maybe cancer .  When the biopsy form breast can estimated it is invasive ductal carcinoma  Fibro adenoma  Fibrocystic change arwajed.com
  • 31. REQUESTED  When you send the biopsy for pathology examination tell in pathology request form for pathology center you send the simple for which kind of examination  H&E  Specially exam  HER2--------0-3 from 0-+1Nig +2 +3 ---+3 positive +2 rep  1-FISH/ Flrocent insitu hybridisation  2-CISH/ Chromogenic in situ hybridisation  ICH/ Immunohistochemistry  Molecular exam  arwajed.com
  • 32. DATE &TIME OF OPERATION  Make clear date and time of operation it is necessary when the operation is done on which time  Date:02/05/2017  Time: 2:30pm:  After the operation the biopsy send to pathology deportment for diagnosis in fixative biopsy will be received on time not late if it is received late without any fixative lost the diagnosis value arwajed.com
  • 33. DOCTOR IDENTIFICATION  Name of doctor  Signature of doctor  Telephone Number of doctor  Email address of doctor arwajed.com
  • 35. ‫اهداف‬‫تثبیت‬ FIXATION GOALS 1-‫جلوگیری‬‫ویا‬ ‫خودبخودی‬ ‫ازتخریب‬‫انساج‬ ‫اتولیز‬ 2.‫نموده‬ ‫جلوگیری‬ ‫انتانات‬ ‫ازمداخله‬ 3.‫شود‬ ‫اسانی‬ ‫به‬ ‫ان‬ ‫کارروی‬ ‫که‬ ‫نسج‬ ‫نمودن‬ ‫سخت‬ ‫جهت‬ 4.‫درنسج‬ ‫نوری‬ ‫تراکم‬ ‫افزایش‬ 5.‫نسج‬ ‫شدن‬ ‫ازجمع‬ ‫جلوگیری‬ 6.‫تلوین‬ ‫مواد‬ ‫به‬ ‫سازکاری‬ 8.‫،رنگ‬ ‫شکل‬ ‫حفظ‬ ‫امکان‬ ‫تاحد‬‫بیوپسی‬ ‫واندازه‬ 7.‫میکند‬ ‫جلوگیری‬ ‫درانساج‬ ‫ازمرگ‬ ‫بعد‬ ‫ازتغیرات‬ ‫درکل‬ arwajed.com
  • 36. ‫کننده‬ ‫تثبیت‬ ‫محلول‬ ‫شده‬ ‫قبول‬ ‫که‬ ‫کننده‬ ‫تثبیت‬ ‫محلول‬ ‫بهترین‬ ‫میباشد‬ ‫فیصد‬ ‫ده‬ ‫فارملین‬ ‫است‬ ‫جهانی‬ arwajed.com
  • 41. ‫فرمالین‬10% ‫بهترین‬ ‫عنوان‬ ‫به‬‫میگردد‬ ‫قرار‬ ‫استفاده‬ ‫مورد‬ ‫ها‬ ‫نمونه‬ ‫دراکثر‬ ‫که‬ ‫است‬ ‫کننده‬ ‫تثبیت‬. ‫محلول‬ ‫غلظت‬ ‫با‬ ‫خالص‬ ‫فرمالین‬40%‫است‬ ‫آب‬ ‫در‬ ‫فرمالدئید‬ ‫گاز‬. ‫فرمالین‬10%‫محلول‬ ‫غلظت‬ ‫با‬4%‫است‬ ‫آب‬ ‫در‬ ‫فرمالین‬ ‫گاز‬. ‫بین‬ ‫رقت‬ ‫با‬ ‫نهایی‬ ‫غلظت‬8-12%‫با‬ ‫ای‬ ‫مالحظه‬ ‫قابل‬ ‫تفاوت‬ ‫و‬ ‫شده‬ ‫پذیرفته‬10%‫ندارد‬. ‫فرمالین‬ ‫تهیه‬ ‫برای‬10%‫مقدار‬ ،‫خالص‬ ‫فرمالین‬ ‫از‬10‫با‬ ‫خالص‬ ‫فرمالین‬ ‫سی‬ ‫سی‬90 ‫گردد‬ ‫می‬ ‫مخلوط‬ ‫آب‬ ‫سی‬ ‫سی‬. arwajed.com
  • 45. ‫فرمالین‬10% ‫حداقل‬ ‫باید‬ ‫فرمالین‬ ‫حجم‬10‫باشد‬ ‫بافت‬ ‫برابر‬. ‫ظ‬‫از‬ ‫بعد‬ ‫که‬ ‫طوری‬ ‫به‬ ،‫باشد‬ ‫داشته‬ ‫بزرگ‬ ‫درب‬ ‫باید‬ ‫نمونه‬ ‫حاوی‬ ‫رف‬ ‫شود‬ ‫آورده‬ ‫در‬ ‫ظرف‬ ‫از‬ ‫راحتی‬ ‫به‬ ‫نمونه‬ ،‫فیکساسیون‬. ‫کند‬ ‫احاطه‬ ‫طرف‬ ‫هر‬ ‫از‬ ‫را‬ ‫نمونه‬ ‫باید‬ ‫فرمالین‬. ‫پوشانده‬ ‫گاز‬ ‫وسیله‬ ‫به‬ ‫بایستی‬ ‫هستند‬ ‫فرمالین‬ ‫در‬ ‫شناور‬ ‫که‬ ‫بزرگ‬ ‫های‬ ‫نمونه‬ ‫شوند‬. ‫گیرند‬ ‫می‬ ‫قرار‬ ‫ظرف‬ ‫کف‬ ‫در‬ ‫که‬ ،‫بزرگ‬ ،‫ای‬ ‫صفحه‬ ،‫سنگین‬ ‫های‬ ‫نمونه‬ ‫برای‬ ‫گیرد‬ ‫قرار‬ ‫نمونه‬ ‫و‬ ‫ظرف‬ ‫کف‬ ‫بین‬ ‫گاز‬ ‫بایستی‬. arwajed.com
  • 46. ‫فرمالین‬10% ‫ماه‬ ‫در‬ ‫ولی‬ ‫است‬ ‫خوب‬ ‫شود‬ ‫اطاق‬ ‫دردرجه‬ ‫اگر‬ ‫کردن‬ ‫تثبیت‬ ‫زمستان‬‫کنیم‬ ‫گرم‬ ‫را‬ ‫فارملین‬ ‫متوانیم‬ ‫را‬ ‫فارملین‬ arwajed.com
  • 47. ‫فواید‬‫فرمالین‬10% ‫ارزان‬ ‫ماندن‬ ‫باقی‬‫نمونه‬‫تخریب‬ ‫بدون‬ ،‫طوالنی‬ ‫مدت‬ ‫به‬ ‫آن‬ ‫در‬ ‫مانند‬ ‫خاص‬ ‫های‬ ‫آمیزی‬ ‫رنگ‬ ‫اکثریت‬ ‫با‬ ‫آن‬ ‫مطابقت‬IHC ‫شدن‬ ‫چملک‬‫حداقل‬‫نمونها‬ ‫حفظ‬DNA‫در‬ ‫فری‬ ‫با‬ ‫فرمالین‬ ‫با‬4‫سانتیگراد‬ ‫درجه‬ ‫است‬ ‫دسترس‬ ‫قابل‬ ‫زیاد‬ ‫است‬ ‫ارزان‬ arwajed.com
  • 48. ‫دیگر‬ ‫مهم‬ ‫نکات‬.... OTHER IMPORTANT POINTS .... 1.‫بیو‬ ‫نکات‬ ‫درتمام‬ ‫مساویآ‬ ‫بشکل‬ ‫باید‬ ‫فارملین‬‫شود‬ ‫منتشر‬ ‫پسی‬ 2-‫شود‬ ‫گذاشته‬ ‫نسج‬ ‫ترازخود‬ ‫کالن‬ ‫کانتینر‬ ‫در‬ ‫باید‬ ‫نسج‬ 3-‫باید‬ ‫کننده‬ ‫تثبیت‬ ‫محلول‬6‫تا‬10‫باشد‬ ‫نسج‬ ‫خود‬ ‫برابر‬ 4-‫باشد‬ ‫شناور‬ ‫کننده‬ ‫تثبیت‬ ‫محلول‬ ‫بین‬ ‫در‬ ‫کانتینر‬ ‫بین‬ ‫در‬ ‫خوبی‬ ‫به‬ ‫باید‬ ‫نسج‬ 5-‫کننده‬ ‫تثبیت‬ ‫محلول‬ ‫بخاطریکه‬ ‫شود‬ ‫شق‬ ‫باید‬ ‫باشد‬ ‫بزرگ‬ ‫نسج‬ ‫که‬ ‫درصورت‬ ‫درنسج‬ ‫بتواند‬ ‫کرده‬ ‫نفوذ‬ ‫بخوبی‬ arwajed.com
  • 49. ‫باشد‬ ‫عملیات‬ ‫اطاق‬ ‫نزدیک‬ ‫شفاخانه‬ ‫داخل‬ ‫در‬ ‫تشخیصه‬ ‫مرکز‬ ‫که‬ ‫بود‬ ‫خواهد‬ ‫بهتر‬ ‫وبه‬ ‫داده‬ ‫قرار‬ ‫کننده‬ ‫تثبیت‬ ‫درمحلول‬ ‫را‬ ‫ان‬ ‫بیوپسی‬ ‫ازگرفتن‬ ‫پس‬ ‫بالفاصله‬ ‫وتازه‬ ‫مناسب‬ ‫ظروف‬ ‫در‬ ‫باید‬ ‫نمونها‬ ‫تشخیص‬ ‫غرض‬ ‫دهیم‬ ‫انتقال‬ ‫پتالوژی‬ ‫بخش‬ ‫شود‬ ‫گذاشته‬ ‫ای‬ ‫شیشه‬ ‫یا‬ ‫و‬ ‫پالستیکی‬ ‫شود‬ ‫داده‬ ‫انتقال‬ ‫تشخیصه‬ ‫مرکز‬ ‫به‬ ‫نمونها‬ ‫تاخیر‬ ‫کدام‬ ‫بدون‬ ‫که‬ ‫شود‬ ‫کوشش‬ ‫تاخیر‬ ‫کدام‬ ‫اگر‬ ‫درصورت‬‫بیو‬ ‫درانتقال‬‫بیوپسی‬ ‫درفاملین‬ ‫باید‬ ‫بدهد‬ ‫رخ‬ ‫پسی‬ ‫شود‬ ‫جلوگیری‬ ‫دران‬ ‫ازمرگ‬ ‫بعد‬ ‫تاازتغیرات‬ ‫شود‬ ‫گذاشته‬ ‫شود‬ ‫گذاشته‬ ‫یخچال‬ ‫دربین‬ ‫نمونه‬ ‫کننده‬ ‫تثبیت‬ ‫محلول‬ ‫نبودن‬ ‫درصورت‬ ‫ولی‬ arwajed.com
  • 50. ‫شود‬ ‫فرستاده‬ ‫شود‬ ‫انداخته‬ ‫کالن‬ ‫ظرف‬ ‫یک‬ ‫بین‬ ‫در‬ ‫نمونه‬ ‫که‬ ‫شود‬ ‫کوشش‬ ‫از‬ ‫نیست‬ ‫شفاخانه‬ ‫داخل‬ ‫در‬ ‫ظرف‬ ‫امکانات‬ ‫اگر‬‫پایوازمریض‬‫خواسته‬ ‫بیاورد‬ ‫بیرون‬ ‫از‬ ‫را‬ ‫کالن‬ ‫ظرف‬ ‫یک‬ ‫که‬ ‫شود‬ ‫میباشد‬ ‫ذیل‬ ‫نواقص‬ ‫داری‬ ‫شود‬ ‫انداخته‬ ‫نمونه‬ ‫درپالستیک‬ ‫اگر‬ 1-‫شود‬ ‫پاره‬ ‫راه‬ ‫در‬ ‫ان‬ ‫انتقان‬ ‫جریان‬ ‫در‬ ‫پالستیک‬ ‫دارد‬ ‫امکان‬ 2-‫نور‬ ‫با‬ ‫زود‬ ‫بسیار‬ ‫گیرد‬ ‫قرار‬ ‫افتاب‬ ‫مستقیم‬ ‫شعاع‬ ‫مقابل‬ ‫در‬ ‫پالستیک‬ ‫اگر‬ ‫میشود‬ ‫پزیری‬ ‫رنگ‬ ‫پروسه‬ ‫تغیر‬ ‫سبب‬ ‫و‬ ‫میکند‬ ‫تعامل‬ ‫افتاب‬ 3-‫داده‬ ‫قرار‬ ‫است‬ ‫دوا‬ ‫ان‬ ‫اثرات‬ ‫دران‬ ‫که‬ ‫دیگر‬ ‫دوا‬ ‫بوتل‬ ‫بین‬ ‫در‬ ‫نباید‬ ‫نمونه‬ ‫معقطع‬ ‫تغیر‬ ‫سبب‬ ‫میگرد‬ ‫را‬ ‫مواد‬ ‫همان‬ ‫رنگ‬ ‫نمونه‬ ‫که‬ ‫بخاطریکه‬ ‫شود‬ ‫میشود‬ arwajed.com
  • 51. ‫پتالوژی‬ 6-‫شود‬ ‫انجام‬ ‫جراح‬ ‫داکتر‬ ‫ازطرف‬ ‫کالن‬ ‫کتله‬ ‫شق‬ ‫میکند‬ ‫کمک‬ ‫تشخیص‬ ‫در‬ ‫کی‬ ‫مهم‬ ‫نکات‬ ‫بخاطرحفظ‬ 7-‫و‬ ‫شده‬ ‫نسج‬ ‫نکروز‬ ‫سبب‬ ‫تواند‬ ‫نه‬ ‫نفوذکرده‬ ‫نسج‬ ‫دربین‬ ‫اگرمحلول‬ ‫چرا‬ ‫میدهد‬ ‫دست‬ ‫از‬ ‫را‬ ‫تشخیصه‬ ‫ارزش‬ arwajed.com
  • 52. ‫میشود‬ ‫گرفته‬ ‫جراح‬ ‫توسط‬ ‫نمونه‬ ‫به‬ ‫و‬ ‫داده‬ ‫قرار‬ ‫کننده‬ ‫تثبیت‬ ‫محلول‬ ‫در‬ ‫را‬ ‫ان‬ ‫جراح‬ ‫توسط‬ ‫کتله‬ ‫برداشتن‬ ‫از‬ ‫بعد‬ ‫که‬ ‫است‬ ‫داکتر‬ ‫وظیفه‬ ‫تشخیص‬ ‫جهت‬ ‫بالفاصله‬ ‫شود‬ ‫داده‬ ‫انتقال‬ ‫تشخیصه‬ ‫مرکز‬ ‫مکمل‬ ‫تاریخچه‬ ‫کند‬ ‫ترتیب‬ ‫راجستر‬ ‫ورق‬ ‫تا‬ ‫داده‬ ‫دستور‬ ‫خود‬ ‫دستان‬ ‫زیر‬ ‫به‬ ‫داده‬ ‫قرار‬ ‫کننده‬ ‫تثبیت‬ ‫مناسب‬ ‫محلول‬ ‫در‬ ‫را‬ ‫نمونه‬ ‫نماید‬ ‫درج‬ ‫دران‬ ‫مریض‬ ‫وری‬ ‫و‬ ‫ترباشد‬ ‫بزرگ‬ ‫نمونه‬ ‫خود‬ ‫از‬ ‫درکانتینرکه‬ ‫فیصد‬ ‫ده‬ ‫فارملین‬ ‫مانند‬ ‫گردد‬ ‫درج‬ ‫مریض‬ ‫به‬ ‫راجع‬ ‫معلومات‬ ‫تمام‬ ‫کانتینر‬ ‫مربوطه‬ ‫وارد‬ ‫یا‬ ‫بخش‬ ‫عملیات‬ ‫جستر،تاریخ‬ ‫را‬ ‫،ولد،سن،شماره‬ ‫اسم‬......... arwajed.com
  • 53. ‫به‬ ‫راجع‬ ‫معلومات‬ ‫تمام‬ ‫باید‬ ‫مریض‬ ‫پتالوژی‬ ‫معاینات‬ ‫درخواستی‬ ‫ورق‬ ‫در‬ ‫پری‬ ‫خانه‬ ‫داکتر‬ ‫خود‬ ‫توسط‬ ‫باید‬ ‫مریض‬ ‫درخواستی‬ ‫ورق‬ ‫شود‬ ‫درج‬ ‫مریض‬ ‫را‬ ‫مریض‬ ‫چون‬ ‫جراح‬ ‫داکتر‬ ‫بخاطریکه‬ ‫دیگر‬ ‫صحی‬ ‫کارمند‬ ‫کدام‬ ‫توسط‬ ‫نه‬ ‫شود‬ ‫میتواند‬ ‫دارد‬ ‫معلومات‬ ‫موارد‬ ‫،وغیره‬ ‫شکل‬ ‫حدود‬ ‫مورد‬ ‫در‬ ‫کرده‬ ‫عملیات‬ ‫درست‬ ‫بشکل‬ ‫نماید‬ ‫ارایه‬ ‫را‬ ‫خوبتر‬ ‫معلومات‬ ‫اسم،ولد،سن،جنس،محلول‬ ‫گردد‬ ‫درج‬ ‫مریض‬ ‫ورق‬ ‫در‬ ‫باید‬ ‫که‬ ‫معلومات‬ ‫و‬ ‫مریض‬ ‫قبلی‬ ‫،عملیات‬ ‫مریض‬ ‫قبلی‬ ‫،تداویی‬ ‫دیگر‬ ‫مریضی‬ ‫نمونه،تاریخچه‬ ‫اکر‬ ‫میشود‬ ‫داده‬ ‫انتقال‬ ‫نمونه‬ ‫ان‬ ‫در‬ ‫که‬ ‫محلول‬ ‫وذکر‬ ‫عملیات‬ ‫تاریخ‬ ‫همچنان‬ ‫گردد‬ ‫ذکر‬ ‫مریض‬ ‫معاینات‬ ‫درخواستی‬ ‫ورق‬ ‫در‬ ‫باید‬ ‫باشد‬ ‫استخوان‬ ‫نمونه‬ ‫است‬ ‫متفاوت‬ ‫کامآل‬ ‫استخوان‬ ‫باال‬ ‫کار‬ ‫شیوه‬ ‫چون‬ ‫است‬ ‫استخوان‬ ‫نمونه‬ ‫که‬ arwajed.com