VisualSonics has introduced a revolutionary micro-ultrasound and photoacoustic imaging system that allows researchers to collect a plethora of important data over the lifespan of animals, thereby significantly reducing the number of animals needed.
Molecular imaging uses radiotracers and imaging modalities like PET and SPECT to non-invasively image biological processes at the molecular and cellular level. It has applications in both diagnostic imaging to locate targeted molecules involved in disease, as well as therapy to treat disease targets. PET provides higher resolution images while SPECT requires less equipment but has lower resolution. Both modalities detect emissions from radiotracers to construct 2D or 3D images showing the distribution of chemicals in the body.
Contrast-enhanced ultrasound uses microbubbles and ultrasound to improve visualization of blood vessels and assessment of vascular perfusion. Microbubbles are spherical gas-filled shells approximately 1-4 micrometers in size composed of materials such as albumin or lipids. When combined with ultrasound imaging, they enhance the contrast between blood pools and surrounding tissues. This technique is useful for evaluation of organ perfusion and differentiation of benign and malignant lesions. Targeted microbubbles also show promise for molecular imaging applications.
This document summarizes a retrospective analysis of contrast enhanced ultrasound (CEUS) examinations performed at Södra Älvsborgs Sjukhus from 2011-2014. It analyzes the conclusiveness of CEUS for various organs like liver, kidneys, and spleen. It finds that CEUS was more accurate than CT or MRI in many cases, able to identify more lesions or characterize lesions that other modalities could not. It discusses areas for expanding the use of CEUS in the future, including for trauma, small parts, and extravascular applications. The document aims to evaluate the hospital's experience with CEUS to improve its use and planning going forward.
(December 2, 2021) The Bench to Bedside Series: Preclinical Cancer Research w...Scintica Instrumentation
Overview:
The goal of this webinar will be to provide a high-level overview of the various stages of preclinical cancer research and discuss the role that innovative instrumentation can play in moving science forward.
To better understand how to treat and control cancer, researchers start by investigating the basics – the cells, molecules, and genes that make up the human body. This type of study, which is often referred to as basic or discovery research, aims to understand the underlying mechanisms contributing to cancer growth and spread. This knowledge is an essential starting point for developing future diagnostic tests and treatment strategies.
After finding an innovative idea that works in cells, researchers need to take their studies to the next level by employing animal models that have similar biology to humans. Animal models have helped scientists make some of the most important cancer discoveries over the years. Furthermore, preclinical imaging technologies allow researchers to perform longitudinal animal studies that are noninvasive leaving the underlying biology intact so that one can track changes throughout the entire disease process.
It was previously thought that the journey from bench to bedside was unidirectional, starting with discovery research and moving towards clinical trials. However, in the last decade, it has become crucial for basic scientists and clinicians to work together towards finding innovative solutions that will positively impact patient care.
After attending this webinar, we hope you will have a better understanding of the preclinical workflow needed to push an idea from bench to bedside as well as some of the key equipment that is needed along the way.
This webinar series will be hosted by Drs. Katie Parkins and Tyler Lalonde, both of which have extensive experience in translational research areas including oncology, neuroscience, molecular imaging, and drug development.
In this webinar we will discuss the following topics:
• Introduction To Cancer Research
• What does “Bench to Bedside” mean?
• In vitro characterization
• Rapid throughput screening
• Quantitative tools
• Moving towards translation
Multimodality Molecular Imaging – An Overview With Special Focus on PET/CTApollo Hospitals
Multimodality molecular imaging combines multiple imaging modalities to provide enhanced visualization of biological processes. Positron emission tomography (PET) is particularly useful for molecular imaging as it can radiolabel biological molecules to image specific targets or pathways. PET combined with computed tomography (CT) or magnetic resonance imaging (MRI) provides highly accurate anatomical and functional information by precisely aligning PET and anatomical images. These hybrid PET/CT and PET/MRI systems utilize the strengths of each modality and offer opportunities to study molecular biology and disease in novel ways.
In this webinar, Katie will discuss the role hypoxia plays in disease progression and treatment response, specifically in cancer. She will also dive into the various molecular imaging technologies that can be used to visualize and assess hypoxia in preclinical cancer models. Some modalities that will be covered include magnetic resonance imaging (MRI), positron emission tomography (PET), and optical imaging.
Topics to be covered:
What is hypoxia?
Is there a link between hypoxia and cancer?
What imaging modalities can be used to visualize hypoxia in vivo?
What are the advantages and limitations of each technique?
What are some applications of hypoxia imaging?
Hypoxia has been shown to influence many facets of cancer including tumor growth, treatment response, and metastatic potential. Thus, the ability to noninvasively visualize hypoxia in vivo may be critical to understanding the underlying tumor biology, guiding treatment plans, and determining prognosis in the clinic.
Many different modalities have been used for preclinical hypoxia imaging. While some techniques have been around for decades and have extensive data behind them, others are emerging technologies that aim to overcome existing limitations in the field. Choosing the right modality can be challenging and is dependent on experimental conditions including tumor model, animal strain, and the desired measurement, as each technique will target a different aspect of hypoxia. In this webinar, we will discuss some molecular imaging techniques that can be used to visualize and characterize tumor hypoxia including MRI, PET, optical, and PAI. We will compare the various options, discuss the advantages and limitations of each approach, and show some examples of how scientists are using these techniques within their research.
References
Rebecca A. D’Alonzo, Suki Gill, Pejman Rowshanfarzad, Synat Keam, Kelly M. MacKinnon, Alistair M. Cook & Martin A. Ebert (2021) In vivo noninvasive preclinical tumor hypoxia imaging methods: a review, International Journal of Radiation Biology, 97:5, 593-631, DOI: 10.1080/09553002.2021.1900943
Nanotechnology and its Application in Cancer TreatmentHasnat Tariq
Nanotechnology
Nanomaterials
Nanostructures
Nanoparticles
Unexpected Optical Properties of Nanoparticles
Synthesis of Nanoparticles
Nanotechnology in Cancer Treatment
Role of Sulfur NPs in Cancer Treatment
Human Tumour Cell Lines Used in Research
Ehrlich ascites carcinoma (EAC)
Sulfur Nanoparticles Preparation
MTT Assay
Sulphorhodamine-B (SRB) Assay
Median lethal dose (LD 50)
Experimental design
FT-IR Characterization of Sulfur Nanoparticles
SEM Characterization of Sulfur Nanoparticles
EDS Characterization of Sulfur Nanoparticles
XRD Characterization of Sulfur Nanoparticles
Chemical Studies on Sulfur Nanoparticles In Vitro
Biochemical investigations
Conclusion
Applications of Nanoparticles in cancer treatment
Nanoshells
Nano X-Ray therapy
Drug Delivery by Nanoparticles
The Molecular Imaging Laboratory at Howard University provides state-of-the-art imaging equipment including high resolution MRI systems for small animal and clinical research. The lab aims to train students and foster multidisciplinary research using imaging to study disease processes and investigate new treatments. Areas of research include in vivo MRI and optical imaging of disease models in small animals, as well as molecular imaging of biological processes and developing new imaging probes and nanoparticles.
Molecular imaging uses radiotracers and imaging modalities like PET and SPECT to non-invasively image biological processes at the molecular and cellular level. It has applications in both diagnostic imaging to locate targeted molecules involved in disease, as well as therapy to treat disease targets. PET provides higher resolution images while SPECT requires less equipment but has lower resolution. Both modalities detect emissions from radiotracers to construct 2D or 3D images showing the distribution of chemicals in the body.
Contrast-enhanced ultrasound uses microbubbles and ultrasound to improve visualization of blood vessels and assessment of vascular perfusion. Microbubbles are spherical gas-filled shells approximately 1-4 micrometers in size composed of materials such as albumin or lipids. When combined with ultrasound imaging, they enhance the contrast between blood pools and surrounding tissues. This technique is useful for evaluation of organ perfusion and differentiation of benign and malignant lesions. Targeted microbubbles also show promise for molecular imaging applications.
This document summarizes a retrospective analysis of contrast enhanced ultrasound (CEUS) examinations performed at Södra Älvsborgs Sjukhus from 2011-2014. It analyzes the conclusiveness of CEUS for various organs like liver, kidneys, and spleen. It finds that CEUS was more accurate than CT or MRI in many cases, able to identify more lesions or characterize lesions that other modalities could not. It discusses areas for expanding the use of CEUS in the future, including for trauma, small parts, and extravascular applications. The document aims to evaluate the hospital's experience with CEUS to improve its use and planning going forward.
(December 2, 2021) The Bench to Bedside Series: Preclinical Cancer Research w...Scintica Instrumentation
Overview:
The goal of this webinar will be to provide a high-level overview of the various stages of preclinical cancer research and discuss the role that innovative instrumentation can play in moving science forward.
To better understand how to treat and control cancer, researchers start by investigating the basics – the cells, molecules, and genes that make up the human body. This type of study, which is often referred to as basic or discovery research, aims to understand the underlying mechanisms contributing to cancer growth and spread. This knowledge is an essential starting point for developing future diagnostic tests and treatment strategies.
After finding an innovative idea that works in cells, researchers need to take their studies to the next level by employing animal models that have similar biology to humans. Animal models have helped scientists make some of the most important cancer discoveries over the years. Furthermore, preclinical imaging technologies allow researchers to perform longitudinal animal studies that are noninvasive leaving the underlying biology intact so that one can track changes throughout the entire disease process.
It was previously thought that the journey from bench to bedside was unidirectional, starting with discovery research and moving towards clinical trials. However, in the last decade, it has become crucial for basic scientists and clinicians to work together towards finding innovative solutions that will positively impact patient care.
After attending this webinar, we hope you will have a better understanding of the preclinical workflow needed to push an idea from bench to bedside as well as some of the key equipment that is needed along the way.
This webinar series will be hosted by Drs. Katie Parkins and Tyler Lalonde, both of which have extensive experience in translational research areas including oncology, neuroscience, molecular imaging, and drug development.
In this webinar we will discuss the following topics:
• Introduction To Cancer Research
• What does “Bench to Bedside” mean?
• In vitro characterization
• Rapid throughput screening
• Quantitative tools
• Moving towards translation
Multimodality Molecular Imaging – An Overview With Special Focus on PET/CTApollo Hospitals
Multimodality molecular imaging combines multiple imaging modalities to provide enhanced visualization of biological processes. Positron emission tomography (PET) is particularly useful for molecular imaging as it can radiolabel biological molecules to image specific targets or pathways. PET combined with computed tomography (CT) or magnetic resonance imaging (MRI) provides highly accurate anatomical and functional information by precisely aligning PET and anatomical images. These hybrid PET/CT and PET/MRI systems utilize the strengths of each modality and offer opportunities to study molecular biology and disease in novel ways.
In this webinar, Katie will discuss the role hypoxia plays in disease progression and treatment response, specifically in cancer. She will also dive into the various molecular imaging technologies that can be used to visualize and assess hypoxia in preclinical cancer models. Some modalities that will be covered include magnetic resonance imaging (MRI), positron emission tomography (PET), and optical imaging.
Topics to be covered:
What is hypoxia?
Is there a link between hypoxia and cancer?
What imaging modalities can be used to visualize hypoxia in vivo?
What are the advantages and limitations of each technique?
What are some applications of hypoxia imaging?
Hypoxia has been shown to influence many facets of cancer including tumor growth, treatment response, and metastatic potential. Thus, the ability to noninvasively visualize hypoxia in vivo may be critical to understanding the underlying tumor biology, guiding treatment plans, and determining prognosis in the clinic.
Many different modalities have been used for preclinical hypoxia imaging. While some techniques have been around for decades and have extensive data behind them, others are emerging technologies that aim to overcome existing limitations in the field. Choosing the right modality can be challenging and is dependent on experimental conditions including tumor model, animal strain, and the desired measurement, as each technique will target a different aspect of hypoxia. In this webinar, we will discuss some molecular imaging techniques that can be used to visualize and characterize tumor hypoxia including MRI, PET, optical, and PAI. We will compare the various options, discuss the advantages and limitations of each approach, and show some examples of how scientists are using these techniques within their research.
References
Rebecca A. D’Alonzo, Suki Gill, Pejman Rowshanfarzad, Synat Keam, Kelly M. MacKinnon, Alistair M. Cook & Martin A. Ebert (2021) In vivo noninvasive preclinical tumor hypoxia imaging methods: a review, International Journal of Radiation Biology, 97:5, 593-631, DOI: 10.1080/09553002.2021.1900943
Nanotechnology and its Application in Cancer TreatmentHasnat Tariq
Nanotechnology
Nanomaterials
Nanostructures
Nanoparticles
Unexpected Optical Properties of Nanoparticles
Synthesis of Nanoparticles
Nanotechnology in Cancer Treatment
Role of Sulfur NPs in Cancer Treatment
Human Tumour Cell Lines Used in Research
Ehrlich ascites carcinoma (EAC)
Sulfur Nanoparticles Preparation
MTT Assay
Sulphorhodamine-B (SRB) Assay
Median lethal dose (LD 50)
Experimental design
FT-IR Characterization of Sulfur Nanoparticles
SEM Characterization of Sulfur Nanoparticles
EDS Characterization of Sulfur Nanoparticles
XRD Characterization of Sulfur Nanoparticles
Chemical Studies on Sulfur Nanoparticles In Vitro
Biochemical investigations
Conclusion
Applications of Nanoparticles in cancer treatment
Nanoshells
Nano X-Ray therapy
Drug Delivery by Nanoparticles
The Molecular Imaging Laboratory at Howard University provides state-of-the-art imaging equipment including high resolution MRI systems for small animal and clinical research. The lab aims to train students and foster multidisciplinary research using imaging to study disease processes and investigate new treatments. Areas of research include in vivo MRI and optical imaging of disease models in small animals, as well as molecular imaging of biological processes and developing new imaging probes and nanoparticles.
This document summarizes various molecular imaging techniques. It discusses how nuclear medicine involves molecular imaging by combining detectable labels with biologically important molecules to assess cellular functions. Positron emission tomography (PET) and single photon emission computed tomography (SPECT) allow noninvasive assessment and quantification of small differences between patients. New molecular imaging agents currently in clinical trials will help transform imaging and therapy by quantifying targets like gene expression, receptors, and tumor metabolism. The document outlines different imaging modalities including PET, SPECT, magnetic resonance imaging, optical imaging, ultrasound, and identifies various biomarkers that can be used as imaging targets.
Opto-acoustic breast imaging is a new diagnostic tool that uses non-ionizing laser pulses and ultrasound detection to generate high-resolution functional images of breast tissue. It can help detect breast cancer at early stages by identifying tumors as small as 2 mm based on their optical absorption properties and blood oxygenation levels. This technique offers greater specificity than ultrasound alone and may help reduce unnecessary biopsies by providing additional information to rule out benign lesions.
(June 28th, 2021) Webinar: Applications Overview Using Low Field MRI To Study...Scintica Instrumentation
About Rutgers Molecular Imaging Center and the Aspect M2
The Rutgers Molecular Imaging Center received the Aspect M2 in the summer of 2012. With the versatility of the M2, a wide range of studies and over 85000 scans have been completed at the center. We have had conventional scans such as cancers, traumatic brain injuries, contrast agents, and other biologicals. The center has also reviewed items such as flow measurements of toothpaste, water filters in action, and pills breaking down during digestion. Please join us as we review examples of the various scans completed here at Rutgers University.
The Rutgers Molecular Imaging Center received the Aspect M2 in 2012. Since the M2’s arrival, it has been one of the most utilized instruments at our center. As of May 1st, 2021, the instrument has seen over 6900 hours of use generating 450 projects/patient files comprising a total of over 85,000 scans. Since the M2 was installed, a combination of 60 departments (over 100 researchers) from multiple universities and pharmaceutical companies have had scans completed at Rutgers. At least ten users from five different departments have been trained over the years to independently run the MRI. This webinar will highlight some of the scans completed here at the center.
One major focus of the center has been the brain and central nervous system. Some examples are the following: Longevity scans of Traumatic Brain Injury have been completed using the Mouse Brain Coil. For another TBI investigator project, leakage into the ventricles post-injury has been tracked with the use of positive contrast agents. Negative contrast agents have been utilized to track stem cells traveling from the lower vertebrae up the spine to points of injury or accumulating around damage in the brain. Changes to the brain following low levels of radiation exposure have been observed using the rat coil. Spontaneous paralysis was reviewed when phenotyping a new mouse strain. A wound healing model tracked the progress of recovery within a spinal injury model.
A second important focus involves cancer models actively scanned at Rutgers. Multiple investigators track the progress of lung cancer and its metastasis. Another investigator tracked cancers in the liver through an interesting negative contrast technique. One Cancer Institute of NJ investigator tracked prostate growth in the same mice for up to two years. Yet another research group tracked an aggressive lymphoma on a weekly basis. Other interesting cancer scans that have been recorded are tumors in the bone, under the tongue, breast duct, uterus, just to name a few of the ones scanned at the center.
This document summarizes oncology and pulmonary services offered by a specialty contract research organization. The CRO provides microCT imaging and analysis, histology, immunohistochemistry, and software services to support preclinical drug development. Key services highlighted include microCT analysis of bleomycin-induced lung fibrosis, vascular casting, histopathology, and a cloud-based platform for managing and exploring experimental data.
Ultrasound contrast agents rely on the different ways sound waves are reflected at interfaces between substances. Commercially available contrast media are gas-filled microbubbles administered intravenously, which have a high echogenicity compared to soft tissues. Contrast-enhanced ultrasound can image blood perfusion in organs and measure blood flow. Microbubbles are around 1-4 μm, similar to red blood cell size, and consist of a gas core surrounded by a lipid shell. Non-targeted contrast agents remain in circulation temporarily, while targeted agents are designed to bind specific molecules expressed in tissues of interest. Contrast imaging techniques include linear and nonlinear methods.
Picturing Science: An overview of Imaging TechnologiesDr. Sima Salahshor
In the past decades imaging technologies are increasingly used to model the dynamics and structure of biological systems. Biomedical imaging is now an integral part of biological and medical sciences and is used in both clinical practice and research. In this session some of the latest imaging technologies were reviewed.
Skeletal radionuclide imaging plays an important role in understanding bone metabolism and evaluating bone abnormalities. Various radiotracers are used including 99mTc-MDP, 18F-NaF, and 18F-FDG. SPECT and PET are employed to produce images showing radiotracer distribution. Normal scans show clear, symmetric uptake throughout the skeleton with increased uptake in joints, while abnormal scans can detect changes in blood flow, osteoblastic activity, or bone destruction.
In Vivo Bioluminescent / Fluorescent Imagingachang07
The presentation is an introduction to using bioluminescent "reagents" to evaluate drug efficacy in tumor models. This presentation briefly highlights one of many research platforms available at Caliper Life Sciences\' Discovery Alliances\' In Vivo Division that performs contract research for the life science community.
- Small Animal In Vivo Imaging (SAIVI) has 25 years of experience developing fluorescent molecules and 45 chemists with expertise in organic and inorganic dyes, ligands, and enzyme substrates.
- SAIVI is now applying its expertise and tools to enable animal imaging to better understand the key characteristics required for effective animal imaging reagents.
- Molecular imaging combines targeting technology from molecular biology with detection technology from imaging instrumentation to image and monitor cellular and animal physiology and function in vivo.
Nanotechnology has applications in the medical field through the use of nano-scale devices called nanorobots. These nanorobots can operate at the scale of individual atoms and molecules to diagnose and treat diseases. Some potential uses include imaging tumors at the nano-scale, targeted drug delivery to specific locations in the body, destruction of cancer cells through generation of radiation or acoustic signals, repair of damaged tissues, and detection and removal of viruses. While promising advances, nanomedicine also faces challenges of high costs and difficulties in practical implementation.
Current and future techniques for cancer diagnosisNitin Talreja
This document discusses various nanotechnology approaches for cancer diagnosis, including the use of gold nanoparticles, quantum dots, carbon nanotubes, and nanoflares. Gold nanoparticles can be used for detection through techniques like dynamic light scattering and surface plasmon coupling. Quantum dots and carbon nanotubes can also be functionalized for ultrasensitive detection of cancer biomarkers. Emerging tools like nanoflares allow for detection of genetic targets associated with cancer within living cells. Overall, nanotechnology enables low detection limits and early cancer diagnosis.
Near-infrared spectroscopy can identify features of vulnerable atherosclerotic plaques by analyzing biochemical composition without staining or reagents. Studies of human and rabbit plaques in vitro and in vivo demonstrated high sensitivity and specificity for detecting lipid pools and thin caps. A catheter-based near-infrared spectroscopy system was tested in pigs and humans with no complications, allowing identification of high-risk plaques during cardiac catheterization. Further clinical studies are needed to determine if near-infrared spectroscopy can successfully detect vulnerable plaques and stratify cardiac risk in human patients.
Near-infrared spectroscopy can identify features of vulnerable atherosclerotic plaques by analyzing biochemical composition without staining or reagents. Studies of human and rabbit plaques in vitro and in vivo demonstrated high sensitivity and specificity for detecting lipid pools and thin caps. A catheter-based near-infrared spectroscopy system was tested in pigs and humans with no complications, allowing identification of high-risk plaques during cardiac catheterization. Further clinical studies are needed to determine if near-infrared spectroscopy can successfully detect vulnerable plaques and stratify cardiac risk in human patients.
Near-infrared spectroscopy can identify features of vulnerable atherosclerotic plaques by analyzing biological tissue absorbance in the near-infrared wavelength range. Studies from 1998-2001 showed this technique could detect lipid pools, thin caps, and macrophages in human aortic plaques with high sensitivity and specificity. It also identified lipid-rich plaques in human coronary sections and living rabbit models. A percutaneous coronary near-infrared spectroscopy catheter was tested safely in pigs and humans. Additional clinical studies are needed but this technique may help identify vulnerable plaques in the catheterization laboratory.
Near-infrared spectroscopy can identify features of vulnerable atherosclerotic plaques by analyzing biological tissue absorbance in the near-infrared wavelength range. Studies from 1998-2001 showed this technique could detect lipid pools, thin fibrous caps, and macrophages in human aortic plaques with high sensitivity and specificity. It also identified lipid-rich plaques in human coronary arteries and living rabbit models. A percutaneous coronary near-infrared spectroscopy catheter was tested safely in pigs and humans. Additional clinical studies are needed but this technique may help identify vulnerable plaques in the cardiac catheterization laboratory.
This document discusses the potential applications of nanotechnology in cancer diagnosis and treatment. It begins with an overview of nanotechnology and nanomedicine. It then discusses how cancer forms and the various factors that can cause cancer like chemicals, radiation, viruses and lifestyle. The document outlines how nanotechnology can be used to more effectively deliver drugs, detect cancer at an early stage, and treat cancer through approaches like photothermal ablation using gold nanoparticles. It acknowledges challenges like ensuring nanoparticles are biocompatible and non-toxic, but envisions that human clinical trials within the next few years could demonstrate how nanotechnology allows for safer and more targeted cancer treatment.
Lab-on-a-Chip for cancer diagnostics and monitoringstanislas547
This document discusses lab-on-a-chip technology for cancer diagnostics and monitoring. It describes how lab-on-a-chip allows miniaturization of diagnostic tools to fit on a small chip. Examples are given of chips that can detect cancer markers from small samples of blood or other bodily fluids. The document outlines how lab-on-a-chip could provide frequent, non-invasive monitoring of cancer markers to guide treatment and detect recurrence. However, challenges remain in developing control units and integrating all necessary functions like fluid handling and molecular analysis onto a single chip.
Expanding preclinical and histopathology capabilities with MRI technology: a ...Scintica Instrumentation
This free webinar hosted by Scintica Instrumentation reviewed the fundamentals of Magnetic Resonance Histology (MRH) and provided a number of relevant examples. Magnetic Resonance Imaging (MRI) has been used for years in preclinical research to perform in vivo studies allowing for the sensitive detection of pathological changes in soft tissue and to provide quantitative three-dimensional data.It has been used in longitudinal studies to noninvasively monitor the genesis, progression and regression of a wide variety of diseases, reducing the need for interim sacrifice of animals at specified time points, thus allowing the same animal to be used as its own control within a given study. MRH is the use of MR imaging on formalin-fixed tissues for high resolution characterization of tissue structure. It is a highly valuable complimentary adjunct to conventional histopathology, as it permits a thorough examination to be performed through multiple digital slices of an entire organ, while leaving the formalin-fixed specimen intact for subsequent definitive conventional diagnostic histopathology.
Breast cancer research in animal models has long been hindered by the lack of a fast, portable, high resolution, research and animal focused imaging system that can visualize 2D tumor size, 3D tumor volume, neoangiogenesis and blood perfusion in vivo, in real-time and most importantly, non-invasively.
Peter is an experienced accountant skilled in accounts payable, month-end and year-end close processes, and process improvement. He is known to be extremely hard-working, self-directed, and possesses high integrity. Prior recommendations note that Peter consistently delivers high quality work, takes initiative to improve processes, and maintains positive working relationships.
This document summarizes various molecular imaging techniques. It discusses how nuclear medicine involves molecular imaging by combining detectable labels with biologically important molecules to assess cellular functions. Positron emission tomography (PET) and single photon emission computed tomography (SPECT) allow noninvasive assessment and quantification of small differences between patients. New molecular imaging agents currently in clinical trials will help transform imaging and therapy by quantifying targets like gene expression, receptors, and tumor metabolism. The document outlines different imaging modalities including PET, SPECT, magnetic resonance imaging, optical imaging, ultrasound, and identifies various biomarkers that can be used as imaging targets.
Opto-acoustic breast imaging is a new diagnostic tool that uses non-ionizing laser pulses and ultrasound detection to generate high-resolution functional images of breast tissue. It can help detect breast cancer at early stages by identifying tumors as small as 2 mm based on their optical absorption properties and blood oxygenation levels. This technique offers greater specificity than ultrasound alone and may help reduce unnecessary biopsies by providing additional information to rule out benign lesions.
(June 28th, 2021) Webinar: Applications Overview Using Low Field MRI To Study...Scintica Instrumentation
About Rutgers Molecular Imaging Center and the Aspect M2
The Rutgers Molecular Imaging Center received the Aspect M2 in the summer of 2012. With the versatility of the M2, a wide range of studies and over 85000 scans have been completed at the center. We have had conventional scans such as cancers, traumatic brain injuries, contrast agents, and other biologicals. The center has also reviewed items such as flow measurements of toothpaste, water filters in action, and pills breaking down during digestion. Please join us as we review examples of the various scans completed here at Rutgers University.
The Rutgers Molecular Imaging Center received the Aspect M2 in 2012. Since the M2’s arrival, it has been one of the most utilized instruments at our center. As of May 1st, 2021, the instrument has seen over 6900 hours of use generating 450 projects/patient files comprising a total of over 85,000 scans. Since the M2 was installed, a combination of 60 departments (over 100 researchers) from multiple universities and pharmaceutical companies have had scans completed at Rutgers. At least ten users from five different departments have been trained over the years to independently run the MRI. This webinar will highlight some of the scans completed here at the center.
One major focus of the center has been the brain and central nervous system. Some examples are the following: Longevity scans of Traumatic Brain Injury have been completed using the Mouse Brain Coil. For another TBI investigator project, leakage into the ventricles post-injury has been tracked with the use of positive contrast agents. Negative contrast agents have been utilized to track stem cells traveling from the lower vertebrae up the spine to points of injury or accumulating around damage in the brain. Changes to the brain following low levels of radiation exposure have been observed using the rat coil. Spontaneous paralysis was reviewed when phenotyping a new mouse strain. A wound healing model tracked the progress of recovery within a spinal injury model.
A second important focus involves cancer models actively scanned at Rutgers. Multiple investigators track the progress of lung cancer and its metastasis. Another investigator tracked cancers in the liver through an interesting negative contrast technique. One Cancer Institute of NJ investigator tracked prostate growth in the same mice for up to two years. Yet another research group tracked an aggressive lymphoma on a weekly basis. Other interesting cancer scans that have been recorded are tumors in the bone, under the tongue, breast duct, uterus, just to name a few of the ones scanned at the center.
This document summarizes oncology and pulmonary services offered by a specialty contract research organization. The CRO provides microCT imaging and analysis, histology, immunohistochemistry, and software services to support preclinical drug development. Key services highlighted include microCT analysis of bleomycin-induced lung fibrosis, vascular casting, histopathology, and a cloud-based platform for managing and exploring experimental data.
Ultrasound contrast agents rely on the different ways sound waves are reflected at interfaces between substances. Commercially available contrast media are gas-filled microbubbles administered intravenously, which have a high echogenicity compared to soft tissues. Contrast-enhanced ultrasound can image blood perfusion in organs and measure blood flow. Microbubbles are around 1-4 μm, similar to red blood cell size, and consist of a gas core surrounded by a lipid shell. Non-targeted contrast agents remain in circulation temporarily, while targeted agents are designed to bind specific molecules expressed in tissues of interest. Contrast imaging techniques include linear and nonlinear methods.
Picturing Science: An overview of Imaging TechnologiesDr. Sima Salahshor
In the past decades imaging technologies are increasingly used to model the dynamics and structure of biological systems. Biomedical imaging is now an integral part of biological and medical sciences and is used in both clinical practice and research. In this session some of the latest imaging technologies were reviewed.
Skeletal radionuclide imaging plays an important role in understanding bone metabolism and evaluating bone abnormalities. Various radiotracers are used including 99mTc-MDP, 18F-NaF, and 18F-FDG. SPECT and PET are employed to produce images showing radiotracer distribution. Normal scans show clear, symmetric uptake throughout the skeleton with increased uptake in joints, while abnormal scans can detect changes in blood flow, osteoblastic activity, or bone destruction.
In Vivo Bioluminescent / Fluorescent Imagingachang07
The presentation is an introduction to using bioluminescent "reagents" to evaluate drug efficacy in tumor models. This presentation briefly highlights one of many research platforms available at Caliper Life Sciences\' Discovery Alliances\' In Vivo Division that performs contract research for the life science community.
- Small Animal In Vivo Imaging (SAIVI) has 25 years of experience developing fluorescent molecules and 45 chemists with expertise in organic and inorganic dyes, ligands, and enzyme substrates.
- SAIVI is now applying its expertise and tools to enable animal imaging to better understand the key characteristics required for effective animal imaging reagents.
- Molecular imaging combines targeting technology from molecular biology with detection technology from imaging instrumentation to image and monitor cellular and animal physiology and function in vivo.
Nanotechnology has applications in the medical field through the use of nano-scale devices called nanorobots. These nanorobots can operate at the scale of individual atoms and molecules to diagnose and treat diseases. Some potential uses include imaging tumors at the nano-scale, targeted drug delivery to specific locations in the body, destruction of cancer cells through generation of radiation or acoustic signals, repair of damaged tissues, and detection and removal of viruses. While promising advances, nanomedicine also faces challenges of high costs and difficulties in practical implementation.
Current and future techniques for cancer diagnosisNitin Talreja
This document discusses various nanotechnology approaches for cancer diagnosis, including the use of gold nanoparticles, quantum dots, carbon nanotubes, and nanoflares. Gold nanoparticles can be used for detection through techniques like dynamic light scattering and surface plasmon coupling. Quantum dots and carbon nanotubes can also be functionalized for ultrasensitive detection of cancer biomarkers. Emerging tools like nanoflares allow for detection of genetic targets associated with cancer within living cells. Overall, nanotechnology enables low detection limits and early cancer diagnosis.
Near-infrared spectroscopy can identify features of vulnerable atherosclerotic plaques by analyzing biochemical composition without staining or reagents. Studies of human and rabbit plaques in vitro and in vivo demonstrated high sensitivity and specificity for detecting lipid pools and thin caps. A catheter-based near-infrared spectroscopy system was tested in pigs and humans with no complications, allowing identification of high-risk plaques during cardiac catheterization. Further clinical studies are needed to determine if near-infrared spectroscopy can successfully detect vulnerable plaques and stratify cardiac risk in human patients.
Near-infrared spectroscopy can identify features of vulnerable atherosclerotic plaques by analyzing biochemical composition without staining or reagents. Studies of human and rabbit plaques in vitro and in vivo demonstrated high sensitivity and specificity for detecting lipid pools and thin caps. A catheter-based near-infrared spectroscopy system was tested in pigs and humans with no complications, allowing identification of high-risk plaques during cardiac catheterization. Further clinical studies are needed to determine if near-infrared spectroscopy can successfully detect vulnerable plaques and stratify cardiac risk in human patients.
Near-infrared spectroscopy can identify features of vulnerable atherosclerotic plaques by analyzing biological tissue absorbance in the near-infrared wavelength range. Studies from 1998-2001 showed this technique could detect lipid pools, thin caps, and macrophages in human aortic plaques with high sensitivity and specificity. It also identified lipid-rich plaques in human coronary sections and living rabbit models. A percutaneous coronary near-infrared spectroscopy catheter was tested safely in pigs and humans. Additional clinical studies are needed but this technique may help identify vulnerable plaques in the catheterization laboratory.
Near-infrared spectroscopy can identify features of vulnerable atherosclerotic plaques by analyzing biological tissue absorbance in the near-infrared wavelength range. Studies from 1998-2001 showed this technique could detect lipid pools, thin fibrous caps, and macrophages in human aortic plaques with high sensitivity and specificity. It also identified lipid-rich plaques in human coronary arteries and living rabbit models. A percutaneous coronary near-infrared spectroscopy catheter was tested safely in pigs and humans. Additional clinical studies are needed but this technique may help identify vulnerable plaques in the cardiac catheterization laboratory.
This document discusses the potential applications of nanotechnology in cancer diagnosis and treatment. It begins with an overview of nanotechnology and nanomedicine. It then discusses how cancer forms and the various factors that can cause cancer like chemicals, radiation, viruses and lifestyle. The document outlines how nanotechnology can be used to more effectively deliver drugs, detect cancer at an early stage, and treat cancer through approaches like photothermal ablation using gold nanoparticles. It acknowledges challenges like ensuring nanoparticles are biocompatible and non-toxic, but envisions that human clinical trials within the next few years could demonstrate how nanotechnology allows for safer and more targeted cancer treatment.
Lab-on-a-Chip for cancer diagnostics and monitoringstanislas547
This document discusses lab-on-a-chip technology for cancer diagnostics and monitoring. It describes how lab-on-a-chip allows miniaturization of diagnostic tools to fit on a small chip. Examples are given of chips that can detect cancer markers from small samples of blood or other bodily fluids. The document outlines how lab-on-a-chip could provide frequent, non-invasive monitoring of cancer markers to guide treatment and detect recurrence. However, challenges remain in developing control units and integrating all necessary functions like fluid handling and molecular analysis onto a single chip.
Expanding preclinical and histopathology capabilities with MRI technology: a ...Scintica Instrumentation
This free webinar hosted by Scintica Instrumentation reviewed the fundamentals of Magnetic Resonance Histology (MRH) and provided a number of relevant examples. Magnetic Resonance Imaging (MRI) has been used for years in preclinical research to perform in vivo studies allowing for the sensitive detection of pathological changes in soft tissue and to provide quantitative three-dimensional data.It has been used in longitudinal studies to noninvasively monitor the genesis, progression and regression of a wide variety of diseases, reducing the need for interim sacrifice of animals at specified time points, thus allowing the same animal to be used as its own control within a given study. MRH is the use of MR imaging on formalin-fixed tissues for high resolution characterization of tissue structure. It is a highly valuable complimentary adjunct to conventional histopathology, as it permits a thorough examination to be performed through multiple digital slices of an entire organ, while leaving the formalin-fixed specimen intact for subsequent definitive conventional diagnostic histopathology.
Breast cancer research in animal models has long been hindered by the lack of a fast, portable, high resolution, research and animal focused imaging system that can visualize 2D tumor size, 3D tumor volume, neoangiogenesis and blood perfusion in vivo, in real-time and most importantly, non-invasively.
Peter is an experienced accountant skilled in accounts payable, month-end and year-end close processes, and process improvement. He is known to be extremely hard-working, self-directed, and possesses high integrity. Prior recommendations note that Peter consistently delivers high quality work, takes initiative to improve processes, and maintains positive working relationships.
Pulse oximetry has been used for diagnosing cyanosis, dyspnea and tachypnea (Schutz and Saunders., 2001). However, it is limited by accuracy-inhibiting factors, such as; decreased peripheral pulses, body temperature and blood pressure.
Development and Validation of a Combined Photoacoustic Micro-Ultrasound Syste...FUJIFILM VisualSonics Inc.
Photoacoustic (PA) Imaging can estimate the spatial distribution of oxygen saturation (sO2) and total hemoglobin concentration (HbT) in blood, and be co-registered with B-Mode ultrasound images of the surrounding anatomy. This study will focus on the development of a PA imaging mode on a commercially available array based micro-ultrasound (μUS) system that is capable of creating such images.
This document discusses the need for improved imaging systems for breast cancer research using animal models. It introduces a new micro-ultrasound system that allows researchers to non-invasively collect longitudinal data on tumor size, volume, vascularity and perfusion over an animal's lifespan. This significantly reduces the number of animals needed for research. The system has been adopted by leading cancer research institutions and numerous studies have been published demonstrating its ability to accurately track tumor growth and response to therapies.
This document provides a bibliography of top nephrology research papers from 2010 and earlier, focusing on topics related to polycystic kidney disease, acute kidney injury, fibrosis, cyst formation, glomerular disease, target organ damage, regional blood flow in the mouse kidney, renal ischemia, phosphate homeostasis, aortic valve calcification in rats, kidney safety in drug development, imaging the kidney with ultrasound, changes to lymphatic and blood vessel architecture from transgenic expression of Angiopoietin 1 in the liver, and a functional floxed allele of Pkd1 that can be conditionally inactivated in vivo. The bibliography contains 20 research papers published between 2004 and 2010.
In this application, Cellvizio was used to study the neuronal degeneration and regeneration processes in live, anaesthetized, adult Thy1-YFP transgenic mice.
Microdialysis is an integral part of preclinical research to determine extracellular fluid and blood concentrations of metabolites, hormones, drugs, etc, and is often used in quantifying the biochemistry of brain and peripheral tissues. However, it is a molecular-only technique and other imaging modalities are needed to provide the researcher with functional and anatomical information of the animal in vivo.
This document summarizes an application brief about using micro-ultrasound to study cancer angiogenesis. It discusses how micro-ultrasound allows researchers to non-invasively visualize 3D tumor volume, neoangiogenesis, and blood perfusion over time in small animal models. This helps researchers better understand cancer development and anti-angiogenic therapies. VisualSonics' micro-ultrasound systems provide high resolution real-time imaging of tumor growth and vascularity to help reduce the number of animals needed for research.
Tumor angiogenesis is currently one of the key focal points in biomedical research. It is based upon the hypothesis laid out by Judah Folkman in 1971 that neovasculature is needed to support the growth and metastasis of tumors, and thus anti-angiogenic treatment might be an effective way to cure cancer. Genentech’s anti-VEGF-A drug Avastin a great demonstration of this concept, generating more than $2.7 billion of sales in 2008.
Abdominal Aortic Aneurysm (AAA) is a serious and potentially fatal disease that is prevalent in the older population. Scientists are making use of animal models to study the progression of this disease and the effects of therapeutic interventions over longitudinal studies.
This document discusses various applications of nuclear techniques that benefit human life and health across the world. It describes how nuclear techniques are used in healthcare for medical diagnosis through in vivo and in vitro applications like PET scans, bone scans, and radioimmunoassays. It also discusses therapeutic uses like radiotherapy and brachytherapy to treat cancer. Additionally, it outlines how nuclear techniques help with agricultural production, livestock production, pest and disease control, and ensuring food quality and safety.
Application Brief: Tumor Microenvironment Imaging with Photoacoustic TechnologyFUJIFILM VisualSonics Inc.
Photoacoustics (PA) combines optical contrast with the high spatial resolution and deep tissue penetration offered by ultrasound. Such applications are especially beneficial for monitoring tumor development, measuring blood concentration changes within it, and quantifying networks of vasculature formation and carcinoma growth over time.
Application Brief: Tumor Microenvironment Imaging with Photoacoustic TechnologyFUJIFILM VisualSonics Inc.
Combining photoacoustic technology with high-resolution ultrasound projections offered by the Vevo 2100 system provides tremendous benefits for cancer screening. Researchers can now benefit from the combined high-resolution ultrasound and optical contrast ability of the Vevo® LAZR photoacoustic imaging system to achieve clear, deep, images in 2D and 3D for optimal in vivo visualization and quantification of internal anatomy, tumor tissue, and hemodynamics.
In the last decade, there has been a rapid refinement of the diagnosis of neck thyroid lesions, especially in the field of thyroid disease. Ultrasound plays a fundamental role in the management of thyroid nodules and tumors and in the approach to recurring neck problems after thyroidectomy. Specifically, ultrasound examination is the main tool for the indication of fine needle aspiration biopsy (FNA) of thyroid lesions, suspicious cervical lymph nodes and parathyroid glands. Moreover, besides its diagnostic role, ultrasound is currently used as a guidance procedure for ablative treatment of benign and malignant cervical lesions.
This document describes a study that used a portable multiphoton gradient index (GRIN) endoscope to image unprocessed ex vivo human prostate tissue samples obtained from radical prostatectomy patients. The endoscope produced images at subcellular resolution and was able to identify differences between benign and malignant prostate tissue as well as other prostatic and periprostatic tissues. The study aims to evaluate the potential for multiphoton GRIN endoscopy to serve as a diagnostic tool for prostate cancer.
The document discusses various types of radiological equipment and procedures used for diagnostic and interventional purposes. It provides detailed descriptions of common radiological exams including chest X-rays, mammography, CT scans, angiography, myelography, and others. It also covers interventional radiology techniques such as MRI, ultrasound, and nuclear medicine studies. The goal of radiological exams is to produce diagnostic images of the inside of the body to evaluate conditions and guide procedures.
High Precision And Fast Functional Mapping Of Cortical Circuitry Through A No...Taruna Ikrar
Taruna Ikrar, MD., PhD. Author at (High Precision and Fast Functional Mapping of Cortical Circuitry Through a Novel Combination of Voltage Sensitive Dye Imaging and Laser Scanning Photostimulation)
The document discusses various technologies used at the House Ear Institute including genomics, proteomics, and imaging. It describes how researchers are using these tools to study diseases like neurofibromatosis type 2 (NF2) at the molecular level in order to develop personalized treatments and therapies. Maintaining high quality biospecimens is important for enabling various types of research.
This document discusses how nanomedicine uses molecular tools and knowledge of the human body to diagnose, treat, and prevent disease at the molecular level. It provides examples of how nanotechnology can enhance drug solubility and bioavailability, enable imaging for diagnosis via MRI, CT, and PET scans, and allow for targeted drug delivery through passive mechanisms like the EPR effect or active targeting using ligands. Future applications discussed include nanorobots for repairing damage, inducing blood clots, enhancing brain cells, and assisting with dental and surgical procedures. Nanoparticles may also enable more effective vaccine development.
- The document describes a study that used multispectral cystoscopy with different light wavelengths to examine bladder mucosa and tumors.
- When green light was used, blood vessels in the bladder mucosa and submucosa showed up clearly in 3D. Blue light also made vessels visible but less clearly. Under red light, the mucosal surface appeared completely red and vessels could not be distinguished.
- For bladder tumors, green light allowed observation of tumor vessels and assessment of invasion depth, while white light showed intact muscle layer after tumor resection but not blood vessels.
Motion-Based Angiogenesis Analysis_A Simple Method to Quanitfy Blood Vessel G...Joe Lee
This document describes a new method called motion-based angiogenesis analysis (MBAA) to quantify blood vessel growth through the motion of blood cells. The method involves recording a video of the regenerating tissue in zebrafish fins after amputation. Image analysis software is used to analyze the video frames and highlight pixels where motion occurs, revealing all blood vessels. Basic fibroblast growth factor and vascular endothelial growth factor were used to stimulate angiogenesis and an inhibitor was used to suppress it. Both ImageJ and ENVI software produced comparable results quantifying the area of new vasculature formed. This simple, accurate, and cost-effective method provides an easy way to quantify angiogenesis without using fluorescent agents or transgenic zebrafish.
This document describes a depth-resolved near-infrared oximeter device being developed to diagnose necrotizing enterocolitis (NEC) in neonates. NEC is a costly disease with high mortality that currently lacks methods for early diagnosis. The device uses near-infrared spectroscopy to measure tissue oxygen saturation levels at different depths in the intestines. It aims to detect NEC at an earlier stage by identifying lower oxygen levels indicative of tissue ischemia. The document outlines the clinical motivation and need for the device, its design which uses LEDs and detectors to obtain depth-resolved measurements, simulation and testing methods, and future implications for improving NEC diagnosis and outcomes.
The document discusses the "camera in a pill", or capsule endoscopy. It provides a high-level overview of the technology, including that a pill-sized camera is swallowed to take images of the small intestine as it passes through. The camera transmits over 56,000 images wirelessly to a sensor array and data recorder worn on the body. The images can later be reviewed by a doctor on specialized software to diagnose conditions of the small intestine.
This document summarizes the potential for using near infrared spectroscopy (NIRS) to diagnose testicular torsion in a non-invasive manner. It describes a case of a 14-month-old male presenting with left scrotal swelling and pain whose ultrasound and Doppler were equivocal. NIRS was used and found a 6.8% lower tissue saturation index in the left testis compared to the right, correctly identifying a 1080 degree torsion requiring left orchiectomy. Animal and limited human studies support NIRS' ability to distinguish torsed from non-torsed testes by detecting lower oxygen saturation in torsed tissues. NIRS offers a non-invasive, radiation-free alternative to ultrasound for diagn
Nuclear medicine in musculoskeletal disordersfatmahoceny
This document discusses nuclear medicine techniques for evaluating musculoskeletal disorders like rheumatoid arthritis. It provides an overview of normal bone scintigraphy patterns and discusses how nuclear medicine can detect functional changes in RA before structural damage occurs. Specifically, it notes that three-phase bone scintigraphy and SPECT are sensitive for detecting early RA abnormalities and monitoring treatment response. Positron emission tomography using FDG can also quantify joint inflammation and correlate with disease activity measures. Nuclear medicine thus provides functional imaging that complements anatomical imaging for evaluating musculoskeletal diseases.
This document describes a study using spectral-domain optical coherence tomography (SD-OCT) to examine long-term cerebrovascular changes in response to voluntary exercise in rats. 14 rats were assigned to either an exercise or control group. SD-OCT scanning was performed under anesthesia to image the motor cortex at high resolution before and after inducing hypoxia, allowing non-invasive longitudinal assessment. Histology will also be performed and compared to SD-OCT. The results will help explore exercise-induced cerebrovascular plasticity and validate SD-OCT for mapping vascular changes over time.
This document describes a high resolution multi-modal imaging platform that combines ultrasound and photoacoustics. It provides anatomical, functional, and molecular data with superior resolution down to 30 μm. The system has a customizable touchscreen interface and is compact and portable. It can be used for applications in neurobiology, molecular biology, cardiology, and oncology to assess vascularity, perfusion, biomarkers, and response to therapy.
Vevo 3100 - The ultimate preclinical imaging experience. The Vevo 3100 is a new and innovative platform created for the future of imaging. It combines ultra high frequency ultrasound imaging, quantification and education in a convenient all-in-one touchscreen platform.
This document provides a bibliography of top cardiovascular research papers organized by topic. The topics covered include abdominal aortic aneurysm, atherosclerosis, cardiac hypertrophy, cardiac injection, cardiomyopathy, chick embryo, contrast, developmental cardiology, diastolic dysfunction, graft transplantation, Holt-Oram syndrome, Marfan syndrome, myocardial infarction, pulmonary hypertension, rabbit cardiovascular, rat cardiovascular, stem cells, stress echocardiography, and valvular flow & function. For each topic, several of the most influential papers from 2009-2010 are listed with their citation information.
This document provides a bibliography of research papers related to cancer organized by topic. It includes 3-7 references for each of 17 cancer-related topics, such as 3D tumor imaging, angiogenesis, bladder cancer, breast cancer, contrast imaging, and others. The references provided for each topic are journal articles published between 2005-2010 that describe research on imaging, molecular markers, treatment responses, and other aspects of various cancers.
This document provides a protocol for imaging the deep brain of freely moving mice. It describes:
1) Implanting a guide cannula into the mouse skull to access the brain region of interest. This involves drilling holes, inserting screws, and cementing the cannula in place.
2) Precisely positioning the cannula using stereotactic coordinates to target a specific brain structure.
3) Inserting the cannula 300-600 micrometers into the brain depending on the target depth.
White Paper: In vivo Fiberoptic Fluorescence Microscopy in freely behaving miceFUJIFILM VisualSonics Inc.
Fiberoptic fluorescence microscopy (FFM) employs optical fibers as small as 300 micrometers in diameter and offers the ability to image cellular and subcellular processes in deep brain structures including the Ventral Tegmental Area (VTA) and the substantia nigra (Sn).
1. The document describes the Vevo 2100 imaging platform from VisualSonics, which redefines preclinical ultrasound imaging with high resolution, advanced functionality, and quantitative analysis capabilities.
2. It can image a wide range of animal models from embryos to adults, provides anatomical, functional and molecular data non-invasively, and has applications across many research areas.
3. The system offers superior resolution, color and power Doppler, 3D imaging, advanced measurements, and is easy to use with one-button presets and analysis software.
Radiotherapy and chemotherapy aim at killing tumor cells or at least stopping their multiplication. Those therapies have strong limitations: first, their inherent toxicity is not limited to tumoral cells, but also affects healthy tissue; second, only the strongest and most resistant tumoral cells are able to survive, leading to increasingly aggressive tumors.
The document compares the features of the Vevo 2100 and Vevo 770 ultrasound systems, noting that the Vevo 2100 has improved high frequency transducers, multiple focal zones, higher frame rates, access to raw RF data export, improved non-linear contrast and power Doppler imaging, and faster 3D imaging compared to the Vevo 770. The Vevo 2100 also has new features like VevoStrain, color Doppler, simultaneous dual mode viewing, steerable Doppler, ECG-gating, and rabbit imaging that are not available on the Vevo 770.
The document compares the features of the Vevo 2100 and Vevo 770 ultrasound systems, noting that the Vevo 2100 has improved high frequency transducers, multiple focal zones, higher frame rates, access to raw RF data export, improved non-linear contrast and power Doppler imaging, and faster 3D imaging compared to the Vevo 770. The Vevo 2100 also has new features like VevoStrain, color Doppler, simultaneous dual mode viewing, steerable Doppler, ECG-gating, and rabbit imaging that are not available on the Vevo 770.
This 3-sentence summary provides the key details about the protocol:
The protocol describes how to implant a guide cannula in transgenic mice expressing fluorescent proteins to allow insertion of a fiber optic probe for imaging deep brain structures of freely moving mice under anesthesia. The guide cannula is implanted using stereotactic surgery and then a fiber optic probe is inserted through the cannula and lowered to the target brain region under microscope guidance for in vivo imaging of fluorescent cells over multiple time points.
Ischemia - or the lack of blood supply to a tissue - and subsequent reperfusion induces physiological and biochemical changes in the affected tissue and is an important area of study since the damage that occurs as a result is clinically important in diabetes and stroke.
UiPath Test Automation using UiPath Test Suite series, part 6DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 6. In this session, we will cover Test Automation with generative AI and Open AI.
UiPath Test Automation with generative AI and Open AI webinar offers an in-depth exploration of leveraging cutting-edge technologies for test automation within the UiPath platform. Attendees will delve into the integration of generative AI, a test automation solution, with Open AI advanced natural language processing capabilities.
Throughout the session, participants will discover how this synergy empowers testers to automate repetitive tasks, enhance testing accuracy, and expedite the software testing life cycle. Topics covered include the seamless integration process, practical use cases, and the benefits of harnessing AI-driven automation for UiPath testing initiatives. By attending this webinar, testers, and automation professionals can gain valuable insights into harnessing the power of AI to optimize their test automation workflows within the UiPath ecosystem, ultimately driving efficiency and quality in software development processes.
What will you get from this session?
1. Insights into integrating generative AI.
2. Understanding how this integration enhances test automation within the UiPath platform
3. Practical demonstrations
4. Exploration of real-world use cases illustrating the benefits of AI-driven test automation for UiPath
Topics covered:
What is generative AI
Test Automation with generative AI and Open AI.
UiPath integration with generative AI
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
AI 101: An Introduction to the Basics and Impact of Artificial IntelligenceIndexBug
Imagine a world where machines not only perform tasks but also learn, adapt, and make decisions. This is the promise of Artificial Intelligence (AI), a technology that's not just enhancing our lives but revolutionizing entire industries.
Threats to mobile devices are more prevalent and increasing in scope and complexity. Users of mobile devices desire to take full advantage of the features
available on those devices, but many of the features provide convenience and capability but sacrifice security. This best practices guide outlines steps the users can take to better protect personal devices and information.
HCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAUpanagenda
Webinar Recording: https://www.panagenda.com/webinars/hcl-notes-und-domino-lizenzkostenreduzierung-in-der-welt-von-dlau/
DLAU und die Lizenzen nach dem CCB- und CCX-Modell sind für viele in der HCL-Community seit letztem Jahr ein heißes Thema. Als Notes- oder Domino-Kunde haben Sie vielleicht mit unerwartet hohen Benutzerzahlen und Lizenzgebühren zu kämpfen. Sie fragen sich vielleicht, wie diese neue Art der Lizenzierung funktioniert und welchen Nutzen sie Ihnen bringt. Vor allem wollen Sie sicherlich Ihr Budget einhalten und Kosten sparen, wo immer möglich. Das verstehen wir und wir möchten Ihnen dabei helfen!
Wir erklären Ihnen, wie Sie häufige Konfigurationsprobleme lösen können, die dazu führen können, dass mehr Benutzer gezählt werden als nötig, und wie Sie überflüssige oder ungenutzte Konten identifizieren und entfernen können, um Geld zu sparen. Es gibt auch einige Ansätze, die zu unnötigen Ausgaben führen können, z. B. wenn ein Personendokument anstelle eines Mail-Ins für geteilte Mailboxen verwendet wird. Wir zeigen Ihnen solche Fälle und deren Lösungen. Und natürlich erklären wir Ihnen das neue Lizenzmodell.
Nehmen Sie an diesem Webinar teil, bei dem HCL-Ambassador Marc Thomas und Gastredner Franz Walder Ihnen diese neue Welt näherbringen. Es vermittelt Ihnen die Tools und das Know-how, um den Überblick zu bewahren. Sie werden in der Lage sein, Ihre Kosten durch eine optimierte Domino-Konfiguration zu reduzieren und auch in Zukunft gering zu halten.
Diese Themen werden behandelt
- Reduzierung der Lizenzkosten durch Auffinden und Beheben von Fehlkonfigurationen und überflüssigen Konten
- Wie funktionieren CCB- und CCX-Lizenzen wirklich?
- Verstehen des DLAU-Tools und wie man es am besten nutzt
- Tipps für häufige Problembereiche, wie z. B. Team-Postfächer, Funktions-/Testbenutzer usw.
- Praxisbeispiele und Best Practices zum sofortigen Umsetzen
Observability Concepts EVERY Developer Should Know -- DeveloperWeek Europe.pdfPaige Cruz
Monitoring and observability aren’t traditionally found in software curriculums and many of us cobble this knowledge together from whatever vendor or ecosystem we were first introduced to and whatever is a part of your current company’s observability stack.
While the dev and ops silo continues to crumble….many organizations still relegate monitoring & observability as the purview of ops, infra and SRE teams. This is a mistake - achieving a highly observable system requires collaboration up and down the stack.
I, a former op, would like to extend an invitation to all application developers to join the observability party will share these foundational concepts to build on:
“An Outlook of the Ongoing and Future Relationship between Blockchain Technologies and Process-aware Information Systems.” Invited talk at the joint workshop on Blockchain for Information Systems (BC4IS) and Blockchain for Trusted Data Sharing (B4TDS), co-located with with the 36th International Conference on Advanced Information Systems Engineering (CAiSE), 3 June 2024, Limassol, Cyprus.
HCL Notes and Domino License Cost Reduction in the World of DLAUpanagenda
Webinar Recording: https://www.panagenda.com/webinars/hcl-notes-and-domino-license-cost-reduction-in-the-world-of-dlau/
The introduction of DLAU and the CCB & CCX licensing model caused quite a stir in the HCL community. As a Notes and Domino customer, you may have faced challenges with unexpected user counts and license costs. You probably have questions on how this new licensing approach works and how to benefit from it. Most importantly, you likely have budget constraints and want to save money where possible. Don’t worry, we can help with all of this!
We’ll show you how to fix common misconfigurations that cause higher-than-expected user counts, and how to identify accounts which you can deactivate to save money. There are also frequent patterns that can cause unnecessary cost, like using a person document instead of a mail-in for shared mailboxes. We’ll provide examples and solutions for those as well. And naturally we’ll explain the new licensing model.
Join HCL Ambassador Marc Thomas in this webinar with a special guest appearance from Franz Walder. It will give you the tools and know-how to stay on top of what is going on with Domino licensing. You will be able lower your cost through an optimized configuration and keep it low going forward.
These topics will be covered
- Reducing license cost by finding and fixing misconfigurations and superfluous accounts
- How do CCB and CCX licenses really work?
- Understanding the DLAU tool and how to best utilize it
- Tips for common problem areas, like team mailboxes, functional/test users, etc
- Practical examples and best practices to implement right away
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slackshyamraj55
Discover the seamless integration of RPA (Robotic Process Automation), COMPOSER, and APM with AWS IDP enhanced with Slack notifications. Explore how these technologies converge to streamline workflows, optimize performance, and ensure secure access, all while leveraging the power of AWS IDP and real-time communication via Slack notifications.
Best 20 SEO Techniques To Improve Website Visibility In SERPPixlogix Infotech
Boost your website's visibility with proven SEO techniques! Our latest blog dives into essential strategies to enhance your online presence, increase traffic, and rank higher on search engines. From keyword optimization to quality content creation, learn how to make your site stand out in the crowded digital landscape. Discover actionable tips and expert insights to elevate your SEO game.
In the rapidly evolving landscape of technologies, XML continues to play a vital role in structuring, storing, and transporting data across diverse systems. The recent advancements in artificial intelligence (AI) present new methodologies for enhancing XML development workflows, introducing efficiency, automation, and intelligent capabilities. This presentation will outline the scope and perspective of utilizing AI in XML development. The potential benefits and the possible pitfalls will be highlighted, providing a balanced view of the subject.
We will explore the capabilities of AI in understanding XML markup languages and autonomously creating structured XML content. Additionally, we will examine the capacity of AI to enrich plain text with appropriate XML markup. Practical examples and methodological guidelines will be provided to elucidate how AI can be effectively prompted to interpret and generate accurate XML markup.
Further emphasis will be placed on the role of AI in developing XSLT, or schemas such as XSD and Schematron. We will address the techniques and strategies adopted to create prompts for generating code, explaining code, or refactoring the code, and the results achieved.
The discussion will extend to how AI can be used to transform XML content. In particular, the focus will be on the use of AI XPath extension functions in XSLT, Schematron, Schematron Quick Fixes, or for XML content refactoring.
The presentation aims to deliver a comprehensive overview of AI usage in XML development, providing attendees with the necessary knowledge to make informed decisions. Whether you’re at the early stages of adopting AI or considering integrating it in advanced XML development, this presentation will cover all levels of expertise.
By highlighting the potential advantages and challenges of integrating AI with XML development tools and languages, the presentation seeks to inspire thoughtful conversation around the future of XML development. We’ll not only delve into the technical aspects of AI-powered XML development but also discuss practical implications and possible future directions.
Essentials of Automations: The Art of Triggers and Actions in FMESafe Software
In this second installment of our Essentials of Automations webinar series, we’ll explore the landscape of triggers and actions, guiding you through the nuances of authoring and adapting workspaces for seamless automations. Gain an understanding of the full spectrum of triggers and actions available in FME, empowering you to enhance your workspaces for efficient automation.
We’ll kick things off by showcasing the most commonly used event-based triggers, introducing you to various automation workflows like manual triggers, schedules, directory watchers, and more. Plus, see how these elements play out in real scenarios.
Whether you’re tweaking your current setup or building from the ground up, this session will arm you with the tools and insights needed to transform your FME usage into a powerhouse of productivity. Join us to discover effective strategies that simplify complex processes, enhancing your productivity and transforming your data management practices with FME. Let’s turn complexity into clarity and make your workspaces work wonders!
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!SOFTTECHHUB
As the digital landscape continually evolves, operating systems play a critical role in shaping user experiences and productivity. The launch of Nitrux Linux 3.5.0 marks a significant milestone, offering a robust alternative to traditional systems such as Windows 11. This article delves into the essence of Nitrux Linux 3.5.0, exploring its unique features, advantages, and how it stands as a compelling choice for both casual users and tech enthusiasts.
Cosa hanno in comune un mattoncino Lego e la backdoor XZ?Speck&Tech
ABSTRACT: A prima vista, un mattoncino Lego e la backdoor XZ potrebbero avere in comune il fatto di essere entrambi blocchi di costruzione, o dipendenze di progetti creativi e software. La realtà è che un mattoncino Lego e il caso della backdoor XZ hanno molto di più di tutto ciò in comune.
Partecipate alla presentazione per immergervi in una storia di interoperabilità, standard e formati aperti, per poi discutere del ruolo importante che i contributori hanno in una comunità open source sostenibile.
BIO: Sostenitrice del software libero e dei formati standard e aperti. È stata un membro attivo dei progetti Fedora e openSUSE e ha co-fondato l'Associazione LibreItalia dove è stata coinvolta in diversi eventi, migrazioni e formazione relativi a LibreOffice. In precedenza ha lavorato a migrazioni e corsi di formazione su LibreOffice per diverse amministrazioni pubbliche e privati. Da gennaio 2020 lavora in SUSE come Software Release Engineer per Uyuni e SUSE Manager e quando non segue la sua passione per i computer e per Geeko coltiva la sua curiosità per l'astronomia (da cui deriva il suo nickname deneb_alpha).
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...SOFTTECHHUB
The choice of an operating system plays a pivotal role in shaping our computing experience. For decades, Microsoft's Windows has dominated the market, offering a familiar and widely adopted platform for personal and professional use. However, as technological advancements continue to push the boundaries of innovation, alternative operating systems have emerged, challenging the status quo and offering users a fresh perspective on computing.
One such alternative that has garnered significant attention and acclaim is Nitrux Linux 3.5.0, a sleek, powerful, and user-friendly Linux distribution that promises to redefine the way we interact with our devices. With its focus on performance, security, and customization, Nitrux Linux presents a compelling case for those seeking to break free from the constraints of proprietary software and embrace the freedom and flexibility of open-source computing.
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...
Application Brief: Nephrology
1. Application Brief: Nephrology
Introduction
1 in 9 adults in North America suffer from chronic kidney diseases.1 Furthermore, acute
renal failure is not an uncommon adverse event associated with many drugs. It is no wonder that
nephrology research is currently one of the key focal points in biomedical research. One of the
difficulties associated with kidney disease, especially chronic forms, is that in vivo studies must be
used to track disease progression longitudinally. Furthermore, techniques that can visualize renal
blood flow will greatly complement research in this area.
However, nephrology research in animal models has long been hindered by the lack of a
fast, portable, high-resolution, research and animal focused imaging system that can visualize 2D
and 3D kidney images, blood flow and tissue perfusion in vivo, in real-time, and most importantly,
non-invasively. In order to ameliorate this problem, VisualSonics has introduced a revolutionary
micro-ultrasound and photoacoustic imaging system that allows researchers to collect a plethora of
important data over the lifespan of animals, thereby significantly reducing the number of animals
needed.
Numerous satisfied nephrology researchers using Vevo® micro-ultrasound systems, from
institutions such as Johns Hopkins University, Princess Margaret Hospital and NIH are publishing
articles in leading journals such as The American Journal of Pathology, Journal of the American
Society of Nephrology and American Journal of Physiology – Renal Physiology. This is a testament
of the power and versatility of high-resolution micro-ultrasound.
Figure 1 - Mouse kidney 3D Vasculature
A pplication Brief: Nephrology ve r1.2 Page 1
2. Micro-Ultrasound in Nephrology Research
The use of micro-ultrasound in nephrology research has been regarded by leading
researchers as an attractive technique for non-invasive, in vivo imaging of kidney micro-
structures,2 monitoring drug safety in kidneys3 and kidney blood flow and perfusion.4,5,6 Most
research in this area is done using mice, because of their wide availability, variety of strains and
ease of handling. Micro-ultrasound is especially suitable to study mice as they are the perfect size
to take advantage of the maximum resolution Vevo systems offer.
For example, Sullivan et al. recently reported their findings in the American Journal of
Physiology – Renal Physiology of using the Vevo high-resolution system with enhanced contrast
agents to measure renal blood flow.4 The authors were limited by the fact that conventional
methods such as laser Doppler flowmetry are highly invasive and location-restricted and thus
explored the use of micro-ultrasound as an exciting alternative. 4 The authors found that renal
cortical and medullary rates of perfusion in response to endothelin-1 infusion were readily
measured by micro-ultrasound and the results could be verified by laser Doppler flowmetry. They
concluded that the Vevo system offers a rapid, non-invasive way of obtaining hemodynamic
measurements with little risk to experimental subjects.4
Dieterle et al. recently published in Current Opinion in Drug Discovery & Development their
review of different methods to monitor drug-induced nephrotoxicity in preclinical research.3 The
researchers found that the Vevo 770 system is capable of high-resolution, non-invasive imaging of
not only renal artery blood flow, but even microvascular blood flow that is paramount in studies of
changes such as renal artery stenosis and renal remodeling.3 They concluded that micro-ultrasound
is non-invasive, does not require radiation, does not suffer from motion artifacts and thus allows
the performance of longitudinal studies of disease progression and regression. 3
Another very interesting paper was published in Ultrasound in Medicine & Biology by Wang
et al.2 The authors examined the use of micro-ultrasound to image conscious rats.2 Conscious
imaging not only speeds up the screening process, but also decreases confounding variables such
as anesthesia interactions in new drug discovery research. In this example, the authors used the
Vevo system to identify unilateral congenital hydronephrosis of the right kidney in rats, which was
later confirmed via histology.2 They further visualized kidney capsule, cortex, corticomedullary
junction, medulla, papilla, pelvis and hilum vasculature via micro-ultrasound in 24 kidneys.2 It was
concluded that the Vevo system allowed imaging of organ and tissue in the living state where
normal tissue dynamics and physiological processes are intact and that conscious imaging of
animals was possible without causing stress.2
A pplication Brief: Nephrology ve r1.2 Page 2
3. Many other researchers in recent years have also been using Vevo systems as an easily
accessible, applicable, fast and superior way to image kidney structure and blood flow. For a
complete list of publications, please refer to Top Nephrology Research Papers using the Vevo
Systems.
Figure 2 – Rat Kidney Blood Flow
A pplication Brief: Nephrology ve r1.2 Page 3
4. Photoacoustics in Nephrology Research
Photoacoustic imaging has recently been recognized in the field of nephrology research as a
hybrid technology capable of retaining the sensitivity and specificity of optical imaging while
overcoming poor spatial resolution through the depth and scalability of ultrasonic imaging8. Song
and Wang (2008) demonstrated the feasibility of photoacoustic imaging for imaging deep organ
tissues, specifically the kidney in both a rat and rabbit. Because of the depth penetration offered by
ultrasound, combined with the optical imaging abilities of photoacoustic imaging, both anatomical
and functional imaging can be achieved within a tissue on the same plane. Specifically, oxygen
saturation10, blood volume11 and hemoglobin content can be detected & quantified (Figure 3).
Finally, photoacoustics further expands the molecular imaging capabilities for researchers through
the use of nanoparticles and contrast agents, which can be targeted to specific intracellular
receptors and biomarkers. This capability is useful for monitoring disease progression and
therapeutic intervention.
100
1mm
% sO 2
Cortical region
50
Figure 3 –Oxygen saturation map (62% in outlined region) in mouse kidney inhaling 100%
O 2 under anesthesia. Red corresponds to regions to high oxygenation levels, blue low.
Outlined area is an overlay of the cortex.
A pplication Brief: Nephrology ve r1.2 Page 4
5. What Can The Vevo® LAZR Photoacoustic Imaging System Do For Me?
High-frequency ultrasound and photoacoustics are unique imaging modalities for small
animals non-invasive imaging in real-time. With Vevo systems, researchers now have access to a
tool to conduct longitudinal studies in vivo. It has been demonstrated by Loveless et al. that micro-
ultrasound produces images eclipsing MRI resolution and that the two imaging systems can be
combined to validate each other’s results.7 Numerous unique tools for quantification of perfusion,
as well as an open data platform allows the nephrology researcher to have full control over his/her
findings.
Below is a summary of the unique value proposition VisualSonics delivers to researchers with
the Vevo micro ultrasound systems:
1. Non-invasive, in vivo, real-time imaging for processes that happen over a period of time,
such as chronic kidney disease, cyst development and tumor progression.
2. High-spatial resolution up to 30 μm allows for excellent delineation of renal and renal-
related structures such as medulla, cortex, renal vein and artery, ureter, urethra and
bladder in 2D and 3D.
3. Quantification of renal function and flow, such as pulsatility and resistivity indices,
including flow in small vessels of diameter >50 μm.
4. Unique microbubble contrast agents allow quantification of kinetics in the
microcirculation.
5. Targeted contrast agents for quantification of biomarkers involved in inflammation and
angiogenesis.
6. Image-guided injection of cancer cells, stem cells, therapeutic compounds, etc. into the
kidney and/or surrounding tissue without surgery.
7. Gene transfection and enhanced drug delivery into renal cells using the Vevo
SoniGene™ system through sonoporation.
8. Dedicated animal platform to monitor ECG, heart rate, body temperature and respiration
rates.
9. Data export capabilities in excel. Image/movie export capabilities in TIFF, BMP, AVI, etc.
10. Measurement of oxygen saturation for studies in ischemia.
11. Nanoparticle detection and quantification in vivo for targeted cellular and molecular
studies.
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6. The Vevo LAZR Photoacoustic Imaging System
A pplication Brief: Nephrology ve r1.2 Page 6
7. References
1. Craig B. Langman. The Epidemic of Chronic Kidney Disease. MedGenMed 2006; 8(3): 55.
2. Wang YX, Betton G, Floettmann E, Fantham E, Ridgwell G. Imaging kidney in conscious rats
with high-frequency ultrasound and detection of two cases of unilateral congenital
hydronephrosis. Ultrasound Med Biol 2007 Mar;33(3):483-6.
3. Dieterle F, Marrer E, Suzuki E, Grenet O, Cordier A, Vonderscher J. Monitoring kidney safety
in drug development: emerging technologies and their implications. Curr Opin Drug Discov
Devel 2008 Jan;11(1):60-71.
4. Sullivan JC, Wang B, Boesen EI, D'Angelo G, Pollock JS, Pollock DM. Novel use of ultrasound
to examine regional blood flow in the mouse kidney. Am J Physiol Renal Physiol 2009
Jul;297(1):F228-35.
5. Andonian S, Coulthard T, Smith AD, Singhal PS, Lee BR. Real-time quantitation of renal
ischemia using targeted microbubbles: in-vivo measurement of P-selectin expression. J
Endourol 2009 Mar;23(3):373-8.
6. Hakroush S, Moeller MJ, Theilig F, Kaissling B, Sijmonsma TP, Jugold M, Akeson AL,
Traykova-Brauch M, Hosser H, Hähnel B, Gröne HJ, Koesters R, Kriz W. Effects of increased
renal tubular vascular endothelial growth factor (VEGF) on fibrosis, cyst formation, and
glomerular disease. Am J Pathol 2009 Nov;175(5):1883-95.
7. Loveless ME, Whisenant JG, Wilson K, Lyshchik A, Sinha TK, Gore JC, Yankeelov TE.
Coregistration of ultrasonography and magnetic resonance imaging with a preliminary
investigation of the spatial colocalization of vascular endothelial growth factor receptor 2
expression and tumor perfusion in a murine tumor model. Mol Imaging 2009;8(4):187-98.
8. Ntziachristos V. et al. Looking and listening to light: The evolution of whole-body photonic
imaging. Nat. Biotechnol. 2005 (23):313–320
9. Song KH and Wang, LV. Noninvasive photoacoustic imaging of the thoracic cavity and the
kidney in small and large animals. Med Phys. 2008 Oct;35(10):4524-9.
10. Wang X et al. Noninvasive laser-induced photoacoustic tomography for structural and
functional in vivo imaging of the brain. Nat. Biotechnol. 2003 (21):803–6.
11. Stein EW, Maslov K, and Wang LV. Noninvasive mapping of the electrically stimulated
mouse brain using photoacoustic microscopy. Proc. SPIE (2008):685-6.
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