This study compared the efficacy of four mouthwash solutions in reducing plaque and gingivitis over 6 months in a population with good oral hygiene: 1) two low-dose 0.06% chlorhexidine preparations, 2) a commercially available 0.1% chlorhexidine solution, 3) an amine fluoride/stannous fluoride solution, and 4) a water control. All solutions reduced plaque levels, but the 0.1% and 0.06% chlorhexidine solutions were more effective at 3 months. The 0.1% chlorhexidine solution also produced greater reductions in gingivitis than the control. However, it also caused more discoloration than potential alternative solutions.
44 윤정현 diet and the microbial aetiology of dental caries new paradigmsdlawogud
This article reviews the evolving theories around the causes of dental caries over the last 120 years. It discusses how early theories focused on deficiencies in diet, but it is now understood that bacteria like Streptococcus mutans play a key role by metabolizing sugars like sucrose in plaque and lowering the pH. While S. mutans was long thought to be the primary cause, more recent studies find a broader range of bacteria are involved. In addition to sugars, starches can also cause caries. Future caries prevention strategies may focus more broadly on targeting plaque acidity and acidogenic microbes, rather than only S. mutans. An optimal diet remains important to reducing caries risk.
This study compared the efficacy of four mouthwash solutions in reducing plaque and gingivitis over 6 months in a population with good oral hygiene: 1) two low-dose 0.06% chlorhexidine preparations, 2) a commercially available 0.1% chlorhexidine solution, 3) an amine fluoride/stannous fluoride solution, and 4) a water control. All solutions reduced plaque levels, but the 0.1% and 0.06% chlorhexidine solutions were more effective at 3 months. The 0.1% chlorhexidine solution also produced greater reductions in gingivitis than the control. However, it also caused more discoloration than potential alternative solutions.
44 윤정현 diet and the microbial aetiology of dental caries new paradigmsdlawogud
This article reviews the evolving theories around the causes of dental caries over the last 120 years. It discusses how early theories focused on deficiencies in diet, but it is now understood that bacteria like Streptococcus mutans play a key role by metabolizing sugars like sucrose in plaque and lowering the pH. While S. mutans was long thought to be the primary cause, more recent studies find a broader range of bacteria are involved. In addition to sugars, starches can also cause caries. Future caries prevention strategies may focus more broadly on targeting plaque acidity and acidogenic microbes, rather than only S. mutans. An optimal diet remains important to reducing caries risk.
15번 김안석 microleakage assessment of fissure sealant following by a fisuuratomy...dlawogud
This document summarizes a study that evaluated the microleakage of dental fissure sealants following the use of different preparatory techniques. 90 teeth were divided into 3 groups: group A received preparation with a fissurotomy bur, group B received pumice prophylaxis, and group C received no preparation. All groups received etching, adhesive, and sealant. Teeth were thermocycled and stained to assess microleakage. The study found that the fissurotomy bur and pumice prophylaxis groups had lower microleakage scores than the no preparation group, indicating that preparatory techniques enhance sealant adhesion and reduce microleakage.
This document discusses personal protective equipment (PPE) used for infection control, including gloves, masks, and protective eyewear. Gloves should be changed between patients and not worn for long periods. Masks covering the nose and mouth can reduce inhalation of infectious particles from dental aerosols. Protective eyewear with side shields can prevent physical injury and infection from particles ejected from patients' mouths during dental procedures.
2. S. mutans adherence method
S. mutans screening test – Dentoclut SM kit
치면세균막 재형성율 검사
3. S. mutans adherence method
원리
- S. mutans 가 sucrose 함유된 배지에서 자랄 때 유리면에
접착하는 능력에 기초한 타액검사방법
장비
- 타액 수집용 시험관
- 배양 시험관 꽂이
- 1회용 피펫, 배양기
- MSB broth
4. S. mutans adherence method
과정
1. 비자극 타액 0.1ml를 MSB broth 에 주입
2. 타액이 주입된 시험관을 60° 각도로 고정하고 24시간동안
호기성, 37°C에서 배양한다.
3. 성장을 관찰한 후에 상층 배지(supernatant medium)를
제거하고 유리면에 접착된 세포들을 눈으로 관찰하고
다음과 같이 점수를 매긴다.
5. S. mutans screening test –
Dentoclut SM kit
원리
- 치아우식증의 원인균인 S. mutans 만을 선택적으로
배양하여 타액내에 있는 S. mutans 양을 정량화함으로써
치아우식증의 활성정도를 파악
장비
- 배양기
- 배지 및 배양액
- Bacitracin tablet
- Paraffin wax 등
6. S. mutans screening test –
Dentoclut SM kit
• 과정
a. bacitracin plate를 핀셋으로 파란병(배양액이 담긴 용기)에 넣고,
15분 동안 가만히 두었다가 부드럽게 병을 흔들어 준다.
b. 환자에게 paraffin wax를 주어 1분 정도 씹게 한다.
c. 타액용 배지 strip을 구강내에 2/3 정도 삽입한 후 혀 위에서 10번
정도 돌려주며 타액이 충분히 붇히도록 한다. 그 후 입술을 다문
상태에서 배지 strip을 빼내어 과량의 타액을 제거하도록 한다.
d. 구강내 치면세균막의 세균활성도를 확인하기 위한 4부위를
결정하고 기록한다.
e. 각각의 부위에서 치실이나 explorer 등을 이용하여 치면세균막을
채득한 후 해당하는 치면세균막용 배지 strip에 골고루 발라준다.
f. 타액용 배지 strip과 치면세균막용 배지 strip을 결합한 후 배지
뚜껑에 삽입하고 배양액이 담긴 배지 용기에 넣어주고 뚜껑을
닫아준다.
g. 배지가 들어 있는 용기를 배양기에 넣고 37°C 에서 48시간 배양한다.
h. 배지에서 형성된 집락의 양상을 기준표와 비교 판정한다.
8. S. mutans screening test –
Dentoclut SM kit
0 negligible
1 Less than 100,000
2 100,000 to 1,000,000
3 More than 1,000,000
<판정표>
9. 치면세균막 재형성율 검사
목적
1. 철저한 구강위생관리를 습관화 시킨다.
2. 환자 스스로 치면세균막을 제거할 수 있게 해준다.
3. 구강위생관리를 주기적으로 평가할 수 있도록 한다.
원리
1. 치면세마를 실시한다.
2. 치면세마 후 2일간 칫솔질을 하지 않도록 지시한다.
3. 진료대상자의 치아표면을 착색제로 착색시킨다.
4. 치면세균막이 새로 형성된 정도를 관찰, 평가한다.
5. 구강환경관리에 대해 지도한다.