1) The study evaluated the influence of fatty acid composition in feed on the muscle composition of Rana catesbeiana at different development stages. 2) The results showed that feed 1 contained more unsaturated fatty acids like omega-3 and less saturated fatty acids compared to feeds 2 and 3. 3) The muscle of imagos and adults contained higher levels of omega-3 fatty acids and less saturated fatty acids than the feeds, suggesting the frogs retained more unsaturated fatty acids in their tissues.

1. INCORPORATION OF OMEGA 3
FATTY ACIDS IN MUSCLE Rana castesbeiana SHAW,
(1802) "BULL FROG"
Peterson G 1, Albo G, Tavella M., Martinez Zabala S. y Ricci A.
1- PROPIA (Stroke Prevention Program in Argentina)-Scientific Research Commission of the Province of Buenos Aires Faculty of
Medical Sciences. Universidad Nacional de La Plata. Argentina. E-mail: gpeterso@atlas.med.unlp.edu.ar
Introduction: Cardiovascular diseases are the leading cause of
death in our country. According to international health organizations, Objective: The aim of this study was to evaluate the influence of
the diet should be low in salt, sugar and saturated fatty acids and fatty acid composition of the lipid portion of the food used in the
hydrogenated (trans) and rich in unsaturated fatty acids (particularly three stages of development of Rana catesbeiana, on the fatty acid
family omega 3) and fiber. The standard diet in our country is poor in composition of muscle in imagos and adults
omega-3 fatty acids. The Rana catesbeiana, Shaw, 1802, "Bull Frog"is
presented as an alternative to the consumption of meat rich in omega
3, given that feed on the basis of balanced diets enriched with these
fatty acids.
Methods: We analyzed the fatty acid composition of 3 feed formulations: I-flour
to the tadpole stage; II-pellet 1.5 mm in diameter for display and III-3-mm pellet
for adults. Analysis was performed on the fatty acid composition of muscle of
imago and adult frog. The method used was gas chromatography. Measurements
were performed on a Hewlett Packard 5890 gas chromatograph using a capillary
column 50 m in length (CP Sil 88-CHROMPACK). The compositions were
determined by comparison with commercial standard solutions analyzed under
the same conditions.
Results: The calf starter (I) has a low content of saturated fatty acids (28.50%) compared to the others (45.86% and 43.53% for II and III
respectively) .- On the other hand, the starter feed contains 71.16% of unsaturated fatty acid of which 37.40% are Omega 6, Omega 9 are
26.60% and 5.80% are Omega 3 .- Foods II and III have fatty acid contents unsaturated similar to each other (51.61 and 54.26% respectively)
.- Also there are similarities in the content of omega-6 fatty acids (9.96 and 8.86% respectively). The content of Omega 3 fatty acids is
approximately doubled in the case of food III (9.63% for food III and 4.32% for food II). In feed (I) predominate Omega 3 fatty acids short
chain (18: 3 n3), while in food II and III is dominated by long-chain fatty acids (20: 5 n3 and 22: 6 n3). Similar content are Omega 9 fatty acids
from food I and III 26.60% and 29.57% respectively and the food has 36.33% II .- In the muscle samples from both adult frog image as seen a
high content of omega 3 fatty acids (17.22% for adult frog and 15.22% for images). The saturated fatty acid content is significantly lower than
in the food supply (35.54% in image and 32.53% in adults). The content of omega-6 fatty acids is higher than in the ration provided (22.39%
and 15.04% in imagos in adults).
Table 1-Fatty acid composition Table 2-Fatty acid composition
food for frogs frog muscle at different stages of development
Fatty acid (%) I II III Fatty acid Muscle Muscle
(%) imagos adults
14:0 2,63 1,96 3,96 14:0 0,81 1,81
15:0 ND 2,30 1,47 15:0 1,60 2,16
16:0 16,91 28,13 25,38 16:0 2239 21,39
16:1n7 1,36 1,57 6,20 16:1n7 3,48 7,55
17:0 ND 0,72 1,1 17:0 0,40 0,55
18:0 8,96 12,75 12,72 18:0 10,7 6,62
18:1n9t 0,34 2,53 2,21 18:1n9t 1,27 0,81
18:1n9c 26,60 36,33 29,57 18:1n9c 20,13 25,70
18:2n6 36,71 9,96 8,86 18:2n6 18,39 10,90
18:3n3 5,25 0,60 0,16 18:3n3 1,51 1,15
Σ Sat. 28,50 45,86 43,53 Σ Satur. 35,53 32,53
Σ Insat. cis 71,16 51,61 54,26 Σ Insat. cis 63,2 66,66
Σ Insat. n6 37,40 9,96 8,86 Σ Insat. n6 22,39 15,04
Σ Insat. n3 5,80 4,32 9,63 Σ Insat. n3 17,11 18,37
Rel n6/n3 4,58 2,31 0,92 Rel n6/n3 1,3 0,48
Conclusion: The reduced content of saturated fatty acids associated with increased content of omega 6
and 3, in the flesh of both imagos and adults about the food supplied, would infer that the saturated fatty
acids would be used to obtain while the unsaturated power would be retained in the tissues.
Keywords: Rana castesbeiana, saturated fatty acids, omega 3 fatty acids, omega-6 fatty acids.