32.Harshitha B, Subhada B, Mustafa M, Solanki H, Safiya NAM, Tiwari RVC. DNA Laddering to Evaluate Cytogenetic Damage in Patients with Periodontitis. J Int Soc Prev Community Dent. 2019 Sep-Oct;9(5):486-491. doi: 10.4103/jispcd.JISPCD_245_19. eCollection 2019 Sep-Oct. PubMed PMID: 31620382; PubMed Central PMCID: PMC6792315.
Harshitha B, Subhada B, Mustafa M, Solanki H, Safiya NA, Tiwari RV. DNA laddering to evaluate cytogenetic damage in patients with periodontitis. J Int Soc Prevent Communit Dent 2019;9:486-91.
Short-term improvement of clinical parameters and microbial diversity in peri...M ALTAMIMI
Indocyanine green-mediated photodynamic therapy is effective against chronic periodontitis. Here, we evaluated the efficiency of indocyanine green-based adjunctive antimicrobial photodynamic therapy in non- surgical treatment of chronic periodontitis patients
Correlation of Clinical Attachment Level (CAL) and C - Reactive Protein (CRP)...Dr. Anuj S Parihar
Periodontal disease, caused mainly by bacteria, is characterized by inflammation and destruction of the attachment apparatus of the teeth. Periodontitis is a multi-factorial disease with microbial dental plaque as the initiator of periodontal disease. Studies indicate that the periodontal lesion is not strictly a localized process but may lead to systemic alterations in the immune function. The present study intends to evaluate the correlation of clinical attachment level and C-reactive protein levels in
smoker and non-smoker patients with chronic generalized periodontitis. A total of fifty patients were included in the study, and they were divided into two group. Group A consisting of 25 patients who are smokers and they are having chronic generalized periodontitis, while Group B consist of 25 patients who are nonsmokers and having chronic generalized periodontitis. In the study clinical parameters we checked were Oral hygiene index – Simplified (OHI-S), Gingival Index (GI), Probing pocket depth (PPD) and Clinical Attachment level (CAL). Furthermore, CRP was evaluated as well between
Group-A (Smokers with chronic generalized periodontitis) and Group-B (Nonsmokers with chronic generalized periodontitis). The results showed higher OHI – S, PPD, CAL and CRP levels in Group - A (Smokers having chronic generalized periodontitis) than Group - B (Nonsmokers having chronic generalized periodontitis). GI score was higher in Group - B as compared to Group - A. Increased levels of clinical attachment level
(CAL) were seen in smokers suffering from chronic periodontitis. Significantly an increased level of C - reactive protein (CRP) was seen in smokers suffering from chronic periodontitis. Correlation between Clinical attachment level (CAL) and Creactive protein levels (CRP) was very strongly positive and significant. Suggesting, as value of CAL increases, CRP also increases.
Chairside Diagnosis of Periodontal Diseases A ReviewYogeshIJTSRD
A good clinical diagnosis has always been the need of the hour. Proper diagnosis is essential for better treatment and planning of the diseases. Customary clinical estimations utilized for periodontal finding are regularly of restricted convenience as they are pointers of past periodontal illness instead of present disease action. Subsequently, there is a requirement for creating novel demonstrative kits that can identify dynamic diseases, anticipate future illness crisis or movement and assess reaction to periodontal treatment, and treatment encouragement in periodontal patients. In this futuristic era, there has been a tremendous amount of research in the field of diagnostic tools that can be utilized by a dental practitioners and even periodontists in their day to day practice. Distinctive chair side diagnostic kits will be discussed in this paper which will be useful for appropriate diagnosis, assessing the disease anticipation and proper treatment planning. Dr. Sumeet Khanna | Dr. Smarth Khanna | Dr. Parul Goel "Chairside Diagnosis of Periodontal Diseases: A Review" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-3 , April 2021, URL: https://www.ijtsrd.com/papers/ijtsrd37981.pdf Paper URL: https://www.ijtsrd.com/biological-science/allied-sciences/37981/chairside-diagnosis-of-periodontal-diseases-a-review/dr-sumeet-khanna
CONFERENCE PROCEEDINGS
11th International Conference on Biotechnology, Bio Informatics, Bio Medical Sciences and Stem Cell Applications (B3SC), 21-22 Sept, 2016,
Imperial College London, South Kensington Campus | London SW7 2AZd
Effectiveness of Curry Leaf and Mint Decoction on Indigestion among Geriatric...ijtsrd
The prevalence of digestive problems in older people and its link to hospitalization and death in older people living in the community are both poorly understood. The primary sign of ageing is the gradual loss of physiological integrity, which results in dysfunction and increased mortality risk. A Quasi experimental research design and convenience sampling technique was adopted. 60 Geriatric samples with upper gastrointestinal tract problems were selected. Out of which 30 samples were within the experimental group and remaining 30 under control group. The objectives of the study were to assess the level of indigestion among geriatric clients, to assess the effectiveness of curry leaf and mint decoction on indigestion among geriatric clients and to associate the posttest level of indigestion among geriatric clients with selected demographic variables. The study was conducted at Kondancherry village, Thiruvallur district, Tamil Nadu. Pretest was conducted for both the experimental and control group. Demonstrated the control samples with, how to prepare a drink using curry leaves and mint decoction to experimental group and conducted post test for both the experimental group and control group after seven days of interval. The investigator found that the demographic variable marital status 2=8.103, p=0.044 had shown statistically significant association with posttest level of indigestion among geriatrics at p 0.05 level. This type of study can be conducted among large samples. Dayana. B. A. A | Mohanapriya. M | Kingsly. J "Effectiveness of Curry Leaf and Mint Decoction on Indigestion among Geriatric Clients at Kondancherry Village, Thiruvallur District" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-6 , October 2022, URL: https://www.ijtsrd.com/papers/ijtsrd52175.pdf Paper URL: https://www.ijtsrd.com/medicine/nursing/52175/effectiveness-of-curry-leaf-and-mint-decoction-on-indigestion-among-geriatric-clients-at-kondancherry-village-thiruvallur-district/dayana-b-a-a
Harshitha B, Subhada B, Mustafa M, Solanki H, Safiya NA, Tiwari RV. DNA laddering to evaluate cytogenetic damage in patients with periodontitis. J Int Soc Prevent Communit Dent 2019;9:486-91.
Short-term improvement of clinical parameters and microbial diversity in peri...M ALTAMIMI
Indocyanine green-mediated photodynamic therapy is effective against chronic periodontitis. Here, we evaluated the efficiency of indocyanine green-based adjunctive antimicrobial photodynamic therapy in non- surgical treatment of chronic periodontitis patients
Correlation of Clinical Attachment Level (CAL) and C - Reactive Protein (CRP)...Dr. Anuj S Parihar
Periodontal disease, caused mainly by bacteria, is characterized by inflammation and destruction of the attachment apparatus of the teeth. Periodontitis is a multi-factorial disease with microbial dental plaque as the initiator of periodontal disease. Studies indicate that the periodontal lesion is not strictly a localized process but may lead to systemic alterations in the immune function. The present study intends to evaluate the correlation of clinical attachment level and C-reactive protein levels in
smoker and non-smoker patients with chronic generalized periodontitis. A total of fifty patients were included in the study, and they were divided into two group. Group A consisting of 25 patients who are smokers and they are having chronic generalized periodontitis, while Group B consist of 25 patients who are nonsmokers and having chronic generalized periodontitis. In the study clinical parameters we checked were Oral hygiene index – Simplified (OHI-S), Gingival Index (GI), Probing pocket depth (PPD) and Clinical Attachment level (CAL). Furthermore, CRP was evaluated as well between
Group-A (Smokers with chronic generalized periodontitis) and Group-B (Nonsmokers with chronic generalized periodontitis). The results showed higher OHI – S, PPD, CAL and CRP levels in Group - A (Smokers having chronic generalized periodontitis) than Group - B (Nonsmokers having chronic generalized periodontitis). GI score was higher in Group - B as compared to Group - A. Increased levels of clinical attachment level
(CAL) were seen in smokers suffering from chronic periodontitis. Significantly an increased level of C - reactive protein (CRP) was seen in smokers suffering from chronic periodontitis. Correlation between Clinical attachment level (CAL) and Creactive protein levels (CRP) was very strongly positive and significant. Suggesting, as value of CAL increases, CRP also increases.
Chairside Diagnosis of Periodontal Diseases A ReviewYogeshIJTSRD
A good clinical diagnosis has always been the need of the hour. Proper diagnosis is essential for better treatment and planning of the diseases. Customary clinical estimations utilized for periodontal finding are regularly of restricted convenience as they are pointers of past periodontal illness instead of present disease action. Subsequently, there is a requirement for creating novel demonstrative kits that can identify dynamic diseases, anticipate future illness crisis or movement and assess reaction to periodontal treatment, and treatment encouragement in periodontal patients. In this futuristic era, there has been a tremendous amount of research in the field of diagnostic tools that can be utilized by a dental practitioners and even periodontists in their day to day practice. Distinctive chair side diagnostic kits will be discussed in this paper which will be useful for appropriate diagnosis, assessing the disease anticipation and proper treatment planning. Dr. Sumeet Khanna | Dr. Smarth Khanna | Dr. Parul Goel "Chairside Diagnosis of Periodontal Diseases: A Review" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-3 , April 2021, URL: https://www.ijtsrd.com/papers/ijtsrd37981.pdf Paper URL: https://www.ijtsrd.com/biological-science/allied-sciences/37981/chairside-diagnosis-of-periodontal-diseases-a-review/dr-sumeet-khanna
CONFERENCE PROCEEDINGS
11th International Conference on Biotechnology, Bio Informatics, Bio Medical Sciences and Stem Cell Applications (B3SC), 21-22 Sept, 2016,
Imperial College London, South Kensington Campus | London SW7 2AZd
Effectiveness of Curry Leaf and Mint Decoction on Indigestion among Geriatric...ijtsrd
The prevalence of digestive problems in older people and its link to hospitalization and death in older people living in the community are both poorly understood. The primary sign of ageing is the gradual loss of physiological integrity, which results in dysfunction and increased mortality risk. A Quasi experimental research design and convenience sampling technique was adopted. 60 Geriatric samples with upper gastrointestinal tract problems were selected. Out of which 30 samples were within the experimental group and remaining 30 under control group. The objectives of the study were to assess the level of indigestion among geriatric clients, to assess the effectiveness of curry leaf and mint decoction on indigestion among geriatric clients and to associate the posttest level of indigestion among geriatric clients with selected demographic variables. The study was conducted at Kondancherry village, Thiruvallur district, Tamil Nadu. Pretest was conducted for both the experimental and control group. Demonstrated the control samples with, how to prepare a drink using curry leaves and mint decoction to experimental group and conducted post test for both the experimental group and control group after seven days of interval. The investigator found that the demographic variable marital status 2=8.103, p=0.044 had shown statistically significant association with posttest level of indigestion among geriatrics at p 0.05 level. This type of study can be conducted among large samples. Dayana. B. A. A | Mohanapriya. M | Kingsly. J "Effectiveness of Curry Leaf and Mint Decoction on Indigestion among Geriatric Clients at Kondancherry Village, Thiruvallur District" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-6 , October 2022, URL: https://www.ijtsrd.com/papers/ijtsrd52175.pdf Paper URL: https://www.ijtsrd.com/medicine/nursing/52175/effectiveness-of-curry-leaf-and-mint-decoction-on-indigestion-among-geriatric-clients-at-kondancherry-village-thiruvallur-district/dayana-b-a-a
CONFERENCE PROCEEDINGS
11th International Conference on Healthcare, Nursing and Disease Management (HNDM), 21-22 Sept, 2016, London
Imperial College London, South Kensington Campus | London SW7 2AZd
Email: info@iaphlsr.com
http://www.iaphlsr.com
The final protocol (v5.3). Notable changes include:
1) Confirmation of audit standard (Page 6).
2) Refinement of inclusion and exclusion criteria (Page 7)
3) Confirmation of audit status (Appendix C)
4) Refinement of required data fields (Page 19) including definitions (Pages 20-25)
This presentation explains the various controversies in different topics in periodontics. Discusses the controversies in Classification of periodontal diseases,
Diagnosis of periodontal diseases,
Prognosis,
Tooth mobility & splinting,
Gingival curettage one stage full-mouth disinfection versus quadrant SRP,
Systemic antimicrobials in periodontal therapy, Non-surgical versus surgical periodontal therapy,
Postsurgical antimicrobial medication,
Periodontal pack,
Periodontal-endodontic relationship,
Periodontal and systemic diseases,
Implant therapy in periodontally compromised patients.
1) What waswere the outcome(s) of interest in the study If there.docxSONU61709
1) What was/were the outcome(s) of interest in the study? If there is more than one, list all of them
2) Evaluate whether the discussion section of the article adequately addresses the strengths and limitations of the study. Explain your reasoning in complete sentences.
3) Discuss future research studies that would be appropriate given the study findings. Please answer in complete sentences.
4) What are the social change implications of the study results? Please answer in complete sentences.
5) What potential effect modifiers were assessed in the study, and how were they assessed? Please answer in complete sentences
6) What are the limitations of the study, and how do these limitations impact how the study results are interpreted? Please answer in complete sentences.
7) Describe how the study participants were selected for the study, including the inclusion and exclusion criteria used.
8) How were the data on the exposure(s) in the study collected and measured? Were they collected and measured the same way for all participants? Explain using complete sentences.
9) How were the data on the outcome(s) in the study collected and measured? Were they collected and measured the same way for all participants?
10) Given what you have learned in this course about confounding, was the adjustment for confounding adequate, or is residual confounding a concern?
11) What types of selection bias might be affecting this study? Please describe the biases and how they might be affecting the results of the study
12) Please describe the major results of the study. Include the major numerical/statistical results as well as interpretations of them in your own words.
13) Given what you have learned in this course about effect modification, was the assessment adequate? Why or why not? Explain using complete sentences.
14) How well did the study participants represent the larger population from which they were selected? Explain any concerns about generalizability with the study results.
15) What measure of disease frequency, association, and/or impact was calculated to answer the main research question(s)? Please answer in complete sentences.
RESEARCH ARTICLE
Is aspirin associated with diabetic
retinopathy? The Singapore Epidemiology of
Eye Disease (SEED) study
Yuan Shi
1
, Yih-Chung Tham
1,2
, Ning Cheung
1
, Jacqueline Chua
1
, Gavin Tan
1
,
Paul Mitchell
3
, Jie Jin Wang
3,4
, Yin Bun Cheung
4,5
, Ching-Yu Cheng
1,2,4
,
Tien Yin Wong
1,2,4*
1 Singapore Eye Research Institute, Singapore National Eye Centre, Singapore, Singapore, 2 Department of
Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore,
3 Centre for Vision Research, Department of Ophthalmology and Westmead Institute for Medical Research,
University of Sydeney, Sydeney, Australia, 4 Duke-NUS Medical School, Singapore, Singapore, 5 Tampere
Center for Child Health Research, University of Tampere and Tampere University Hospit ...
33.Vohra P, Jamatia K, Subhada B, Tiwari RVC, Althaf MN, Jain C. Correlation of CD4 counts with oral and systemic manifestations in HIV patients. J Family Med Prim Care. 2019 Oct;8(10):3247-3252. doi: 10.4103/jfmpc.jfmpc_767_19. eCollection 2019 Oct. PubMed PMID: 31742150; PubMed Central PMCID: PMC6857402.
Vohra P, Jamatia K, Subhada B, Tiwari RV, Althaf MS, Jain C. Correlation of CD4 counts with oral and systemic manifestations in HIV patients. J Family Med Prim Care 2019;8:3247-52.
Comparison of Frenotomy Techniques for the Treatment of Ankyloglossia in Chil...JaimePlazasRomn
Objective. To compare the effectiveness of conventional (CF),
laser (LF), and Z-plasty (ZF) frenotomies for the treatment
of ankyloglossia in the pediatric population.
Data Sources. A comprehensive search of PUBMED,
EMBASE, and COCHRANE databases was performed.
Review Methods. Relevant articles were independently assessed
by 2 reviewers according to the Preferred Reporting Items for
Systematic Reviews and Meta-Analysis (PRISMA) guidelines.
Results. Thirty-five articles assessing CF (27 articles), LF (4
articles), ZF (3 articles), and/or rhomboid plasty frenotomy
(1 article) were included. A high level of outcome heterogeneity
prevented pooling of data. All 7 randomized controlled
trials (RCTs) were of low quality. Both CF (5 articles with
589 patients) and LF (2 articles with 78 patients) were independently
shown to reduce maternal nipple pain on a visual
analog or numeric rating scale. There were reports of
improvement with breastfeeding outcomes as assessed on
validated assessment tools for 88% (7/8) of CF articles (588
patients) and 2 LF articles (78 patients). ZF improved
breastfeeding outcomes on subjective maternal reports (1
article with 18 infants) only. One RCT with a high risk of
bias concluded greater speech articulation improvements
with ZF compared to CF. Only minor adverse events were
reported for all frenotomy techniques.
Conclusions. Current literature does not demonstrate a clear
advantage for one frenotomy technique when managing children
with ankyloglossia. Recommendations for future research
are provided to overcome the methodological shortcomings in
the literature. We conclude that all frenotomy techniques are
safe and effective for treating symptomatic ankyloglossia.
CONFERENCE PROCEEDINGS
11th International Conference on Healthcare, Nursing and Disease Management (HNDM), 21-22 Sept, 2016, London
Imperial College London, South Kensington Campus | London SW7 2AZd
Email: info@iaphlsr.com
http://www.iaphlsr.com
The final protocol (v5.3). Notable changes include:
1) Confirmation of audit standard (Page 6).
2) Refinement of inclusion and exclusion criteria (Page 7)
3) Confirmation of audit status (Appendix C)
4) Refinement of required data fields (Page 19) including definitions (Pages 20-25)
This presentation explains the various controversies in different topics in periodontics. Discusses the controversies in Classification of periodontal diseases,
Diagnosis of periodontal diseases,
Prognosis,
Tooth mobility & splinting,
Gingival curettage one stage full-mouth disinfection versus quadrant SRP,
Systemic antimicrobials in periodontal therapy, Non-surgical versus surgical periodontal therapy,
Postsurgical antimicrobial medication,
Periodontal pack,
Periodontal-endodontic relationship,
Periodontal and systemic diseases,
Implant therapy in periodontally compromised patients.
1) What waswere the outcome(s) of interest in the study If there.docxSONU61709
1) What was/were the outcome(s) of interest in the study? If there is more than one, list all of them
2) Evaluate whether the discussion section of the article adequately addresses the strengths and limitations of the study. Explain your reasoning in complete sentences.
3) Discuss future research studies that would be appropriate given the study findings. Please answer in complete sentences.
4) What are the social change implications of the study results? Please answer in complete sentences.
5) What potential effect modifiers were assessed in the study, and how were they assessed? Please answer in complete sentences
6) What are the limitations of the study, and how do these limitations impact how the study results are interpreted? Please answer in complete sentences.
7) Describe how the study participants were selected for the study, including the inclusion and exclusion criteria used.
8) How were the data on the exposure(s) in the study collected and measured? Were they collected and measured the same way for all participants? Explain using complete sentences.
9) How were the data on the outcome(s) in the study collected and measured? Were they collected and measured the same way for all participants?
10) Given what you have learned in this course about confounding, was the adjustment for confounding adequate, or is residual confounding a concern?
11) What types of selection bias might be affecting this study? Please describe the biases and how they might be affecting the results of the study
12) Please describe the major results of the study. Include the major numerical/statistical results as well as interpretations of them in your own words.
13) Given what you have learned in this course about effect modification, was the assessment adequate? Why or why not? Explain using complete sentences.
14) How well did the study participants represent the larger population from which they were selected? Explain any concerns about generalizability with the study results.
15) What measure of disease frequency, association, and/or impact was calculated to answer the main research question(s)? Please answer in complete sentences.
RESEARCH ARTICLE
Is aspirin associated with diabetic
retinopathy? The Singapore Epidemiology of
Eye Disease (SEED) study
Yuan Shi
1
, Yih-Chung Tham
1,2
, Ning Cheung
1
, Jacqueline Chua
1
, Gavin Tan
1
,
Paul Mitchell
3
, Jie Jin Wang
3,4
, Yin Bun Cheung
4,5
, Ching-Yu Cheng
1,2,4
,
Tien Yin Wong
1,2,4*
1 Singapore Eye Research Institute, Singapore National Eye Centre, Singapore, Singapore, 2 Department of
Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore,
3 Centre for Vision Research, Department of Ophthalmology and Westmead Institute for Medical Research,
University of Sydeney, Sydeney, Australia, 4 Duke-NUS Medical School, Singapore, Singapore, 5 Tampere
Center for Child Health Research, University of Tampere and Tampere University Hospit ...
33.Vohra P, Jamatia K, Subhada B, Tiwari RVC, Althaf MN, Jain C. Correlation of CD4 counts with oral and systemic manifestations in HIV patients. J Family Med Prim Care. 2019 Oct;8(10):3247-3252. doi: 10.4103/jfmpc.jfmpc_767_19. eCollection 2019 Oct. PubMed PMID: 31742150; PubMed Central PMCID: PMC6857402.
Vohra P, Jamatia K, Subhada B, Tiwari RV, Althaf MS, Jain C. Correlation of CD4 counts with oral and systemic manifestations in HIV patients. J Family Med Prim Care 2019;8:3247-52.
Comparison of Frenotomy Techniques for the Treatment of Ankyloglossia in Chil...JaimePlazasRomn
Objective. To compare the effectiveness of conventional (CF),
laser (LF), and Z-plasty (ZF) frenotomies for the treatment
of ankyloglossia in the pediatric population.
Data Sources. A comprehensive search of PUBMED,
EMBASE, and COCHRANE databases was performed.
Review Methods. Relevant articles were independently assessed
by 2 reviewers according to the Preferred Reporting Items for
Systematic Reviews and Meta-Analysis (PRISMA) guidelines.
Results. Thirty-five articles assessing CF (27 articles), LF (4
articles), ZF (3 articles), and/or rhomboid plasty frenotomy
(1 article) were included. A high level of outcome heterogeneity
prevented pooling of data. All 7 randomized controlled
trials (RCTs) were of low quality. Both CF (5 articles with
589 patients) and LF (2 articles with 78 patients) were independently
shown to reduce maternal nipple pain on a visual
analog or numeric rating scale. There were reports of
improvement with breastfeeding outcomes as assessed on
validated assessment tools for 88% (7/8) of CF articles (588
patients) and 2 LF articles (78 patients). ZF improved
breastfeeding outcomes on subjective maternal reports (1
article with 18 infants) only. One RCT with a high risk of
bias concluded greater speech articulation improvements
with ZF compared to CF. Only minor adverse events were
reported for all frenotomy techniques.
Conclusions. Current literature does not demonstrate a clear
advantage for one frenotomy technique when managing children
with ankyloglossia. Recommendations for future research
are provided to overcome the methodological shortcomings in
the literature. We conclude that all frenotomy techniques are
safe and effective for treating symptomatic ankyloglossia.
60.Srinivasan S, Velusamy G, Munshi MAI, Radhakrishnan K, Tiwari RVC. Comparative Study of Antifungal Efficacy of Various Endodontic Irrigants with and without Clotrimazole in Extracted Teeth Inoculated with Candida albicans. J Contemp Dent Pract. 2020 Dec 1;21(12):1325-1330. PubMed PMID: 33893253.
Mathew P, Kattimani VS, Tiwari RV, Iqbal MS, Tabassum A, Syed KG. New Classification System for Cleft Alveolus: A Computed Tomography-based Appraisal. J Contemp Dent Pract. 2020 Aug 1;21(8):942-948. PubMed PMID: 33568619
Sahu S, Patley A, Kharsan V, Madan RS, Manjula V, Tiwari RVC. Comparative evaluation of efficacy and latency of twin mix vs 2% lignocaine HCL with 1:80000 epinephrine in surgical removal of impacted mandibular third molar. J Family Med Prim Care. 2020 Feb;9(2):904-908. doi: 10.4103/jfmpc.jfmpc_998_19. eCollection 2020 Feb. PubMed PMID: 32318443; PubMed Central PMCID: PMC7113948.
65.Izna, Sasank Kuntamukkula VK, Khanna SS, Salokhe O, Chandra Tiwari RV, Tiwari H. Knowledge and Apprehension of Dental Health Professionals Pertaining to COVID in Southern India: A Questionnaire Study. J Pharm Bioallied Sci. 2021 Jun;13(Suppl 1):S448-S451. doi: 10.4103/jpbs.JPBS_551_20. Epub 2021 Jun 5. PubMed PMID: 34447131; PubMed Central PMCID: PMC8375944.
Vohra P, Belkhode V, Nimonkar S, Potdar S, Bhanot R, Izna, Tiwari RVC. Evaluation and diagnostic usefulness of saliva for detection of HIV antibodies: A cross-sectional study. J Family Med Prim Care. 2020 May;9(5):2437-2441. doi: 10.4103/jfmpc.jfmpc_138_20. eCollection 2020 May. PubMed PMID: 32754516; PubMed Central PMCID: PMC7380795
Mittal S, Hussain SA, Tiwari RVC, Poovathingal AB, Priya BP, Bhanot R, Tiwari H. Extensive pelvic and abdominal lymphadenopathy with hepatosplenomegaly treated with radiotherapy-A case report. J Family Med Prim Care. 2020 Feb;9(2):1215-1218. doi: 10.4103/jfmpc.jfmpc_1125_19. eCollection 2020 Feb. PubMed PMID: 32318498; PubMed Central PMCID: PMC7113973.
36.Kesharwani P, Hussain SA, Sharma N, Karpathak S, Bhanot R, Kothari S, Tiwari RVC. Massive radicular cyst involving multiple teeth in pediatric mandible- A case report. J Family Med Prim Care. 2020 Feb;9(2):1253-1256. doi: 10.4103/jfmpc.jfmpc_1059_19. eCollection 2020 Feb. PubMed PMID: 32318508; PubMed Central PMCID: PMC7113959.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
Basavarajeeyam is an important text for ayurvedic physician belonging to andhra pradehs. It is a popular compendium in various parts of our country as well as in andhra pradesh. The content of the text was presented in sanskrit and telugu language (Bilingual). One of the most famous book in ayurvedic pharmaceutics and therapeutics. This book contains 25 chapters called as prakaranas. Many rasaoushadis were explained, pioneer of dhatu druti, nadi pareeksha, mutra pareeksha etc. Belongs to the period of 15-16 century. New diseases like upadamsha, phiranga rogas are explained.
263778731218 Abortion Clinic /Pills In Harare ,sisternakatoto
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RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
2. 487
Journal of International Society of Preventive and Community Dentistry ¦ Volume 9 ¦ Issue 5 ¦ September-October 2019
Baddam Harshitha, et al.: DNA laddering to evaluate cytogenetic damage
Chronic periodontitis (CGP) is characterized by
slow progression of periodontal attachment loss,
whereas generalized aggressive periodontitis (GAP)
is characterized by rapid loss of periodontal tissues
in otherwise clinically healthy subjects.[5]
Reactive oxygen species (ROS) have gained more
attention, because of their central role to the progression
of inflammatory diseases.[6]
They are involved in normal
cellular metabolism and continuously generated by the
cells in most tissues. The role of ROS in the pathogenesis
of periodontal disease has been illustrated by various
mechanisms such as DNA damage, lipid peroxidation,
protein disruption, and inflammatory cytokines, which
contribute to the periodontal tissue destruction.[7-9]
ROS can cause potential tissue damage by initiating
free radical chain reactions.[10]
Literature suggests
that genetic damage has an important role to play in
various chronic inflammatory and degenerative diseases
such as Parkinson’s disease,[11]
chronic obstructive
pulmonary disease (COPD),[12]
inflammatory bowel
disease,[13]
Behcet’s disease,[14]
coronary artery disease,[15]
and Raynaud’s phenomenon.[16]
The role of instability
of the chromosomes and the propensity of these
diseases has been evaluated with the help of various
cytogenetic tests. Cytogenetic damage in peripheral
blood leukocytes can be assessed using micronucleus
test, flow cytometry, fluorescent assays, COMET assay,
Terminal Deoxynucleotidyl Transferase dUTP Nick
End Labeling (TUNEL) assay, and DNA laddering.[17]
DNA laddering (fragmentation) was first described in
1980 by Andrew Wyllie at the University of Edinburgh
Medical School. It is a sensitive assay that evaluates the
cytogenetic damage that can be visualized as a ladder
pattern of 180–200 base-pairs (bp) due to cleavage of
the ds-DNA by the activation of endonucleases when
run on a standard agarose gel electrophoresis.[18]
The aim
of this study was to evaluate the cytogenetic damage in
chronic and GAP in comparison with healthy controls
by using the DNA-laddering technique.
Materials and Methods
This study was cross-sectional in design, comprising of
45 patients who attended the Outpatient Department
of Periodontics. A total of 74 patients were recruited
for the study. Of these, 14 subjects dropped out, 15 did
not meet the inclusion criteria, and finally 45 subjects
completed the study. Informed consent was obtained
from all the patients. Ethical clearance was obtained
from the Institutional Ethical Committee and Review
Board (Protocol no. SSCDS/2017/42). The sampling
method is the non-probability sampling or convenience
sampling where the subjects were recruited based on the
following criteria [Figure 1].
Inclusion criteria
The inclusion criteria of the study included the
following:
CGP group: This group comprised of 15 systemically
healthy individuals with moderate-to-severe alveolar
bone loss and attachment loss of 5
mm or more in
multiple sites of all four quadrants of the mouth.
GAP group: This group comprised of 15 systemically
healthy individuals with generalized pattern of severe
destruction and attachment loss of at least 5 mm on at
least three permanent teeth other than central incisors
or first molars.
Control group: This group comprised of 15
systemically healthy subjects with no clinical evidence
of periodontal disease who underwent oral prophylaxis
before their treatment procedures such as restorations
and orthodontia.
Exclusion criteria
The exclusion criteria of the study included any kind
of systemic disease (hypertension, diabetes, etc.)
that can change the course of periodontal disease,
pregnancy/lactation, smokers or subjects consuming
Figure 1: Consort diagram showing the patients allocation and the
procedure
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Baddam Harshitha, et al.: DNA laddering to evaluate cytogenetic damage
alcohol, subjects on antibiotics and anti-inflammatory
medications within the past three months, history of any
recentinfections,andsubjectswithhistoryof periodontal
treatment in past six months prior to the study.
Procedure
After recruitment in the study, patients were subjected
to a detailed general and clinical examination and the
data pertaining to all the relevant parameters were
recorded in a proforma specially designed for the study.
The clinical parameters included were probing pocket
depth (PPD), clinical attachment level (CAL), modified
gingival index (MGI), plaque index (PI), and bleeding
index (BI).
For the blood collection, the skin over the antecubital
fossa was disinfected and 5 mL of blood was collected
by venipuncture using 20-gauge needle in a 5-mL
syringe from each patient in all the three groups.
Collected sample is centrifuged at 3,000 rpm for 5 min
to separate white buffy coat that consists of leucocytes.
A series of chemicals are added to lyze the cells and to
dissolve RNA. The final isolated DNA is run on 2%
agarose gel for electrophoresis to visualize the DNA
fragmentation.
The grading of DNA fragmentation as mild (+),
moderate (++), or severe (+++) can be categorized
by the location of the bands that correspond to the
fragmented DNA.
In the mild (+) degree, the location of the bands was
nearer to the wells or in the upper third where the DNA
sample was loaded. In the moderate (++) degree, the
location of the bands was in the middle third, midway
between the wells where the DNA sample was loaded
and the end of the gel. However, in the severe (+++)
degree, the location of the bands was away from the
wells where the DNA sample was loaded and nearer to
the end of the gel in the lower third.[19]
Results
All the clinical parameters in the patients with CGP
and GAP were significantly higher (P < 0.001) than the
control subjects. MGI and PI were significantly higher
in the CGP and the GAP groups than the control
group (P < 0.001). MGI was 1.71 ± 0.40 in Group B
and 1.77 ± 0.26 in Group C. The mean PPD was 1.65 ±
0.15 mm in Group A, 6.57 ± 0.80 mm in Group B, and
6.74 ± 0.45 in Group C, respectively. The mean CAL
was 5.89 ± 0.53 in Group B and 6.56 ± 0.24 in Group C
[Table 1].
Apoptotic DNA fragmentation was observed as a
‘ladder’patternat180–200 bpintervalsinbothCGPand
GAP groups [Figure 2]. The gradation was ‘moderate’
as the location of the bands was in the middle third,
midway between the wells where the DNA sample was
loaded and the end of the gel [Table 2].
In this study, DNA fragmentation was observed in both
CGP and GAP groups suggestive of the cytogenetic
damage. DNA laddering was observed as a ‘ladder’
pattern at 180-bp intervals in the middle third of the
wells in both the CGP and the GAP groups.
Discussion
Periodontitis, a chronic inflammatory disease, is caused
by the periodontopathic bacteria such as Actinobacillus
actinomycetemcomitans, Porphyromonas gingivalis,
Tannerella forsythia, etc. Components of microbial
plaque induce an initial infiltrate of inflammatory cells,
including macrophages, polymorphonuclear leukocytes
(PMNs), and lymphocytes. Microbial components,
lipopolysaccharide (LPS), activate macrophages to
synthesize and release a variety of proinflammatory
molecules, cytokines such as interleukin-1 (IL-
1) and tumor necrosis factor-alpha (TNF-alpha),
prostaglandins, especially prostaglandin E2 (PGE2),
and hydrolytic enzymes. These cytokines exhibit potent
pro-inflammatory and catabolic activities and play a key
roleinperiodontaltissuebreakdownthroughproduction
of collagenolytic enzymes such as metalloproteinases
(MMPs). In the inflammatory environment, these
latent collagenolytic enzymes are activated by ROS
giving rise to increased levels of interstitial collagenases
leading to periodontal breakdown. Evidence links
the association of periodontitis with several systemic
diseases such as diabetes, cardiovascular disease,
COPD, adverse pregnancy outcomes, etc., where
the periodontal inflammation directly contributes to
systemic inflammation, through the release of toxins or
leakage of microbial products into the bloodstream.[20]
These chemical mediators cause destruction of extra
cellular matrix components leading to an increase in
ROS production, which is implicated in the connective
tissue damage sustained during inflammatory diseases.
Table 1: Clinical parameters of patients of tests group and
control group
Clinical parameters Controls CGP GAP
Number of samples 15 15 15
Age (mean ± standard
deviation [SD])
41.8 ± 5.83 45.2 ± 7.07 24.7 ± 3.63
MGI 0.00 ± 0.00 1.71 ± 0.40 1.77 ± 0.26
PI 0.20 ± 0.07 2.02 ± 0.30 1.86 ± 0.26
PD 1.65 ± 0.15 6.57 ± 0.80 6.74 ± 0.45
CAL 0.00 ± 0.00 5.89 ± 0.53 6.56 ± 0.24
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Journal of International Society of Preventive and Community Dentistry ¦ Volume 9 ¦ Issue 5 ¦ September-October 2019
Baddam Harshitha, et al.: DNA laddering to evaluate cytogenetic damage
Neutrophil-mediated tissue injury was evaluated by
Deguchi et al. [21]
where they demonstrated that ROS
causes substantial tissue damage by initiating free
radical chain reactions. DNA damage can be evaluated
by certain tests and methods among which DNA ladder
is the most simple, sensitive, and cost-effective.[22]
DNA fragmentation assay using the agarose gel
electrophoresis is a commonly-used technique for the
detection of apoptosis.[23]
It can easily discriminate
between apoptotic and non-apoptotic (necrotic) modes
of cell death. In most cases, the inter-nucleosomal
cleavage of genomic ds-DNA by the endonucleases,
like caspase-activated DNase (CAD), yields the
characteristic DNA ladder, which is the molecular
hallmark of apoptotic cells.[24,25]
In the DNA ladder
obtained, the pattern observed is that the molecular
weights of the genome fragments are integer multiples
of 180–200
bp length, which are associated with a
nucleosome subunit. Separation of these fragments
by agarose gel electrophoresis and subsequent
visualization, for example, by ethidium bromide
staining, results in a characteristic ‘ladder’ pattern
[Figure 3]. In contrast, a DNA smear is obtained during
agarose gel electrophoresis when genomic fragments of
irregular sizes are induced during cell necrosis.[26]
The results indicated that there are cytogenetic
damages in the periodontitis groups and this correlates
with the literature that suggests that the DNA
mutations and damages were observed in periodontitis
group.[27-29]
Numerous studies showed that the activity
of producing ROS is higher in peripheral blood
neutrophils of patients with periodontitis in contrast
to healthy individuals.[30-32]
There are studies suggesting
a strong association between genetic polymorphism
and periodontitis.[33,34]
These studies suggest that an
increased ROS generation in periodontitis is not only
due to stimulation by pathogens but also is genetically
predisposed.[35]
Cytogeneticdamagewasevaluatedusing
a micronucleus test by Fenech et al.[36]
where they found
the cytogenetic damage in blood and in exfoliated buccal
cells. The involvement of mitochondrial damage with
the disease by the increased ROS production leading to
decreased mitochondrial membrane potential and low
oxygen consumption was put forward by Govindaraj
et al. They also found that mtDNA mutations were
observed in the gingival tissues but were absent in
blood.[37]
Damage of gingival tissues has led to mtDNA
mutations in subjects with CGP and this was evaluated
in a study by Canakci et al.[38]
where they found 5-kb
mtDNA deletion in the gingival tissues of the patients
with CGP in Turkish population. A study evaluated
positive correlation between oxidative stress produced
by periodontitis disease and micronuclei. They
reported that subjects with periodontitis (CP or AgP)
showed an increase in the frequency of micronuclei
that are directly related to DNA damage.[39]
Another
study evaluated oxidative stress-induced apoptosis
Table 2: Grading of the samples of DNA ladder
Group DNA ladder Grading
A- (n = 15) 0(0%) –
B-CGP (n = 15) 15(100%) Moderate
C-GAP (n = 15) 15(100%) Moderate
Figure 3: Diagrammatic representation of DNA fragmentation.
(Source: https://static.bio-rad-antibodies.com/uploads/
DNAFragmentationAntibodies.jpg)
Figure 2: Agarose gel electrophoresis demonstrating the DNA fragmentation
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5. 490 Journal of International Society of Preventive and Community Dentistry ¦ Volume 9 ¦ Issue 5 ¦ September-October 2019
Baddam Harshitha, et al.: DNA laddering to evaluate cytogenetic damage
in H2O2-treated human periodontal ligament cells
(hPDLCs) by TUNEL assay. They found significant
reduction in viability and increased apoptosis in the
hPDLCs which confirms the damage.[40]
In contrast, the findings of few studies did not relate
the role of genetics and oxidative damage in the
pathogenesis of periodontal disease. In a study by
Haritha et al., [41]
no significant differences were found
in the micronuclei frequency in the periodontitis
groups as compared to the controls, thus indicating
a possible lack of cytogenetic damage in peripheral
blood cells. A few other studies also did not report a
positive correlation between the cytogenetic damage
and oxidative stress.[42,43]
Literature suggests that studies evaluated the effect
of initial periodontal therapy on the oxidative stress
markers that detects the DNA damage.[44,45]
Studies
reported that biomarkers of oxidative stress-induced
DNA damage levels like 8-hydroxy-deoxyguanosine
(8-OHdG) were significantly reduced by periodontal
treatment.[46-49]
However, no significant differences in
the local levels of 8-OHdG between individuals with
gingivitisandperiodontitis,[50]
aswellasbetweenpatients
with CGP and GAP [39]
were observed. A study assessed
the frequency of nuclear morphological changes in
gingival epithelial cells by COMET assay in individuals
with periodontitis, before and after periodontal therapy
and compared the morphology to healthy periodontal
tissues. They found that in periodontal disease the
frequency of nuclear morphological changes increased
in gingival epithelial cells and after the periodontal
therapy there was a reduction of the number.[51]
Literature suggests the certitude of the DNA damage by
the ROS released by chronic inflammatory conditions
like metabolic diseases as well.[52,53]
Therefore, to the
best of our knowledge, this study is presumed to be
the first of its kind to evaluate cytogenetic damage of
ds-DNA of the nucleus in peripheral leucocytes using
DNA laddering in periodontitis. Further studies with
larger sample size are required to evaluate the genetic
makeup and the predisposition of the ds-DNA for the
correlation of the intra group variation between CGP
and GAP groups and to assess DNA damage in the
periodontal tissues by DNA-laddering technique.
Financial support and sponsorship
Nil.
Conflicts of interest
There are no conflicts of interest.
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