What is Linux?
Command-line Interface, Shell & BASH
Popular commands
File Permissions and Owners
Installing programs
Piping and Scripting
Variables
Common applications in bioinformatics
Conclusion
What is Linux?
Command-line Interface, Shell & BASH
Popular commands
File Permissions and Owners
Installing programs
Piping and Scripting
Variables
Common applications in bioinformatics
Conclusion
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2023comp90024_linux.pdf
1. Linux on Spartan
COMP90024 Cluster and Cloud Computing
University of Melbourne, 21st
March 2023
lev.lafayette@unimelb.edu.au
2. Prerequisites and CLI Reasons
An account on Spartan and membership to a project is
required.
An SSH (secure shell client) is required. This is typically
already installed on Mac and Linux systems. For MS-
Windows putty or mobaxterm are options.
The command line interface (CLI) is “close to the metal”, and
resource efficient. It is also powerful and, with scripting, frees
up time and effort for the user.
Knowing the Linux CLI is essential for any serious
researcher!
3. SSH and Logging In
Access the logon node with SSH command; e.g.,
ssh lev@spartan.hpc.unimelb.edu.au
Process can be improved with SSH configuration files and
“passwordless SSH” that uses key-exchange for
authentication. Agent-forwarding provides additional
convenience.
https://dashboard.hpc.unimelb.edu.au/ssh/
4. File System and Environment
"Everything in the UNIX system is a file" (Kernighan & Pike,
1984, 41).
Login will take the user to /home/$USERID
https://swcarpentry.github.io/shell-novice/fig/standard-
filesystem-hierarchy.svg
Core environment commands;
whoami : print user id
pwd : print working directory
ls : List the directory contents
date : print system date and time
5. Shell Shortcuts
~ Home directory
. Current working directory
.. One directory level closer to root
/ root directory, directory delimiter
^r Traverse command history or use navigation keys
^L Clear the screen; equivalent of the “clear” command.
Tab completion provides navigation and command options
e.g.,
$ ls /usr/local/common/Int
Interact/ IntroLinux/
6. Command Options and Manual
Commands are modular and robust and come with a default
execution. Alternative execution is invoked with options;
<command> -<abbreviation> or <command> --<full-word>
For example:
ls -lart : Directory listing, all files, reverse time of modification
ls -lash : Directory listing, all files, size is in human format
Hundreds of commands, thousands of options! Fortunately,
there is a manual :)
man ls or info ls : Summary of “ls” command and options.
whatis ls : Short description.
man -k compiler or apropos compiler : Search man pages.
man man: The manual page for manual pages.
7. Pipes and Redirects
The pipe is typically used to redirect the output of one
command as the input to another for further processing.
Multiple commands can be piped in sequence to develop
some very specific outcomes.
who -u | less : List of users logged in, piped to less (viewer)
who | wc -l : Count lines of users logged in
Output and input can redirected to/from a file rather than
standard output or standard input. Output redirects can be
appended.
w > list.txt : Redirect to list.txt
w >> list.txt : Redirect and append to list.txt
8. Files and Text Editing
Filenames can include any characters except the forward
slash (directory delimiter) or null. A good habit to avoid non-
printing characters (e.g., spaces)! Consider using
snake_case, kebab-case, or CamelCase, instead.
The “touch” command creates a file with no content e.g.,
touch LIST.TXT
Filenames are case sensitive; list.txt, LIST.TXT are different.
File types can be determined by the file command (e.g., file
list.txt).
Three main text editors available; nano (easy), emacs
(feature-rich), and vim (powerful).
9. Local Copying
To copy files from a remote system to a local system one can
use tools like wget, git, cURL, scp, or rsync. For example:
wget https://swcarpentry.github.io/shell-novice/fig/standard-
filesystem-hierarchy.svg
wget -r --no-parent https://swcarpentry.github.io/shell-novice/
To copy files and directories within a system use the cp
command. Common options include -r (recursive), -a (archive
permissions), -u (update, copy only if source is newer).
cp /usr/local/common/IntroLinux/gattaca.txt .
cp -r /usr/local/common/IntroLinux
10. Remote Copying
To copy files between systems use scp/stfp or rsync. GUI
clients are available (e.g., Filezilla). Again, passwordless
SSH and SSH client files are useful!
scp lev@spartan.hpc.unimelb.edu.au:gattaca.txt .
scp -r lev@spartan.hpc.unimelb.edu.au:IntroLinux .
The rsync utility provides a fast way to keep two collections of
files "in sync" by tracking changes. This example keeps
permissions (-a), is verbose (-v), compresses between
source and destination (-z), and only syncs if the source is
newer (--update)
rsync -avz --update workfiles/
lev@spartan.hpc.unimelb.edu.au:files/workfiles
11. Remote Copying
To copy files between systems use scp/stfp or rsync. GUI
clients are available (e.g., Filezilla). Again, passwordless
SSH and SSH client files are useful!
scp lev@spartan.hpc.unimelb.edu.au:gattaca.txt .
scp -r lev@spartan.hpc.unimelb.edu.au:IntroLinux .
The rsync utility provides a fast way to keep two collections of
files "in sync" by tracking changes. This example keeps
permissions (-a), is verbose (-v), compresses between
source and destination (-z), and only syncs if the source is
newer (--update)
rsync -avz --update workfiles/
lev@spartan.hpc.unimelb.edu.au:files/workfiles
12. Creating Directories, Moving Files
Directories can be created with the mkdir (make directory)
command. Multiple directories can be created at the same
time with space delimiting the separate directories.
The mv (move) command will move (and rename) a file.
There are options for -f (force), -i (interactive), and -n (no
clobber). If more than one of these is selected only the last
one is applied.
Example:
mkdir BRAF
nano gattaca.txt (make a single character change)
mv gattaca.txt BRAF/
scp -r BRAF lev@spartan.hpc.unimelb.edu.au:
13. File Differences
The -l option is ls gives a timestamp for modification.
Differences in content can be determined by the diff
command. Or, with a side-by-side representation with sdiff.
Further, there is the comm command, which compares two
files sorted line by line.
ls -l gattaca.txt BRAF/gattaca.txt
diff gattaca.txt BRAF/gattaca.txt
sdiff gattaca.txt BRAF/gattaca.txt
comm gattaca.txt BRAF/gattaca.txt
Note that the diff command also gives the sequence of
commands for the very old UNIX editor, ed. This is still used
for invoking patch files!
14. File Searches, Globbing
On many Linux systems one can use the locate command to
find files; but often disabled a large shared system like
Spartan!
Instead, use the “find” command; very powerful, many
options. The following examples also use wildcards (*, all
files and ?, single character) and ranges.
find . -name "*.log"
find . -name "comsol?.log"
find . -name "comsol[1-3].log"
The term “glob” is short for “global” in the UNIX function
glob().
15. Searches Within Files
Searching within a file for a string of text is achieved with
grep (“global search for regular expression and print”). Often
used with “strong” quotes to protect from command
substitution or variables.
The following command is grep, with the option -i for "case-
insensitive", followed by the regular expression 'ATEK'.
grep -i 'ATEK' BRAF/*
touch braf/file1
grep -i 'ATEK' BRAF/*
16. Deletions
Deleting files actually means deleting files on the CLI! There
is no "trashcan" or "recycle bin" or anything like that.
Files can be deleted with the "rm" (remove) command. Empty
directories can be deleted with "rmdir" (remove directory),
however the directory must be empty.
A very powerful shortcut is rm -rf (remove, recursive, force).
rm gattaca.txt
rmdir braf/
rm -rf braf/
Think before running rm -rf – and then think again!
17. This Matters
BRAF is a human gene that makes a protein (imaginatively)
named B-Raf. This protein is involved in sending signals
inside cells, which are involved in directing cell growth.
In 2002, it was shown to be faulty (mutated) in human
cancers. In particular, the difference between the two files
"ATVKSRWSGS" and "ATEKSRWSGS" is the difference that
leads to susceptibility to metastatic melanoma.