PBL Assay Science sells interferons, cytokines, growth factors, antibodies, ELISA kits, cell-based assays, and also performs contract research for pharmaceutical, biotechnology and academic researchers.
Ultrasensitive cytokine and biomarker assay services 2015stephenaparent
This document describes ultrasensitive cytokine and biomarker assay services using two single molecule detection technologies: Singulex Erenna® and Quanterix SimoaTM. More than 40 assays are available using Singulex, which employs magnetic beads, washing, fluorescent labeling, and single molecule counting. Assay characterization is shown for IL-1β, demonstrating sensitivity down to picogram-per-milliliter levels. Quanterix Simoa uses a similar magnetic bead approach with single molecule arrays to enable over 30 assays with high sensitivity.
PBL Assay Science is a biotechnology company that has worked for over 24 years to develop assays and proteins to help researchers solve difficult protein quantification problems. The company was founded in 1990 and has 35 employees based in New Jersey. It produces ELISA and multiplex assay kits, recombinant proteins, antibodies, and provides custom assay services, with a focus on interferons and cytokines.
The document describes the development and use of AlphaLISA assays to characterize monoclonal antibody biotherapeutics. AlphaLISA is a homogeneous bead-based immunoassay that provides advantages over ELISA, including shorter assay times without washing steps. New AlphaLISA assays have been developed for detecting fucosylated IgG, host cell proteins, and quantifying therapeutic antibodies. Data shows the assays offer sensitive and precise detection of analytes during biotherapeutic development and characterization.
Top selling kits 2016 of Elabscience including 139 best selling kits and references published by customers who used in their biology experiments. Turn to page 7 you will know more tips about ELISA kits.
09.elisa kits for iga glomerulonephritis researchElabscience
IgA glomerulonephritis,also known as IgA nephropathy,Berger diseas,or synpharyngitic glomerulonephritis, is a disease of the kidney,specifically it is a form of glomerulonephritis or an inflammation of the glomeruli of the kidney.The classic presentation is episodic hematuria, which usually starts within a day or two of a non-specific upper respiratory tract infection.Less commonly gastrointestinal or urinary infection can be the inciting agent.More info visit: http://www.elabscience.com
ELISA Kits for Signal Transduction ResearchElabscience
Signal transduction plays an important role in activating cellular functions, cell growth, cell differentiation and cell proliferation. Elabscience provides hundreds of ELISA Kits coated with signal transduction targets for you to chose, which may help you in the related research fields. More info visit: http://www.elabscience.com
1. A multiplex sandwich ELISA array was developed to simultaneously measure multiple cytokine levels in cell culture supernatant samples.
2. The array was used to profile the induction of cytokines over time in human immune cells stimulated with PMA and ionomycin.
3. Large amounts of IL-2, IFN-gamma and TNF-alpha were induced at 6 hours, while IL-4, IL-5, IL-10 and IL-13 levels increased more over 24-48 hours, demonstrating the ability to monitor the cytokine response kinetics.
02 elisa kits for malignant pleural effusion researchElabscience
Malignant pleural effusion is a condition in which cancer causes an
abnormal amount of fluid to collect between the thin layers of tissue
(pleura) lining the outside of the lung and the wall of the chest
cavity. Lung cancer and breast cancer account for about 50-65% of
malignant pleural effusions. Other common causes include pleural
mesothelioma and lymphoma.More info visit: http://www.elabscience.com
Ultrasensitive cytokine and biomarker assay services 2015stephenaparent
This document describes ultrasensitive cytokine and biomarker assay services using two single molecule detection technologies: Singulex Erenna® and Quanterix SimoaTM. More than 40 assays are available using Singulex, which employs magnetic beads, washing, fluorescent labeling, and single molecule counting. Assay characterization is shown for IL-1β, demonstrating sensitivity down to picogram-per-milliliter levels. Quanterix Simoa uses a similar magnetic bead approach with single molecule arrays to enable over 30 assays with high sensitivity.
PBL Assay Science is a biotechnology company that has worked for over 24 years to develop assays and proteins to help researchers solve difficult protein quantification problems. The company was founded in 1990 and has 35 employees based in New Jersey. It produces ELISA and multiplex assay kits, recombinant proteins, antibodies, and provides custom assay services, with a focus on interferons and cytokines.
The document describes the development and use of AlphaLISA assays to characterize monoclonal antibody biotherapeutics. AlphaLISA is a homogeneous bead-based immunoassay that provides advantages over ELISA, including shorter assay times without washing steps. New AlphaLISA assays have been developed for detecting fucosylated IgG, host cell proteins, and quantifying therapeutic antibodies. Data shows the assays offer sensitive and precise detection of analytes during biotherapeutic development and characterization.
Top selling kits 2016 of Elabscience including 139 best selling kits and references published by customers who used in their biology experiments. Turn to page 7 you will know more tips about ELISA kits.
09.elisa kits for iga glomerulonephritis researchElabscience
IgA glomerulonephritis,also known as IgA nephropathy,Berger diseas,or synpharyngitic glomerulonephritis, is a disease of the kidney,specifically it is a form of glomerulonephritis or an inflammation of the glomeruli of the kidney.The classic presentation is episodic hematuria, which usually starts within a day or two of a non-specific upper respiratory tract infection.Less commonly gastrointestinal or urinary infection can be the inciting agent.More info visit: http://www.elabscience.com
ELISA Kits for Signal Transduction ResearchElabscience
Signal transduction plays an important role in activating cellular functions, cell growth, cell differentiation and cell proliferation. Elabscience provides hundreds of ELISA Kits coated with signal transduction targets for you to chose, which may help you in the related research fields. More info visit: http://www.elabscience.com
1. A multiplex sandwich ELISA array was developed to simultaneously measure multiple cytokine levels in cell culture supernatant samples.
2. The array was used to profile the induction of cytokines over time in human immune cells stimulated with PMA and ionomycin.
3. Large amounts of IL-2, IFN-gamma and TNF-alpha were induced at 6 hours, while IL-4, IL-5, IL-10 and IL-13 levels increased more over 24-48 hours, demonstrating the ability to monitor the cytokine response kinetics.
02 elisa kits for malignant pleural effusion researchElabscience
Malignant pleural effusion is a condition in which cancer causes an
abnormal amount of fluid to collect between the thin layers of tissue
(pleura) lining the outside of the lung and the wall of the chest
cavity. Lung cancer and breast cancer account for about 50-65% of
malignant pleural effusions. Other common causes include pleural
mesothelioma and lymphoma.More info visit: http://www.elabscience.com
Heat stress affects the kinetics of olfactory responses in rats. The study found that exposing rats to high temperatures of 45°C for 25 minutes significantly increased their body temperatures and caused faster rise and decay times of electrical responses in the olfactory epithelium to odorants. Heat stress also increased blood levels of lipopolysaccharides and free vesicles but did not change most cytokine levels, except for an increase in IL-10. This suggests heat stress causes strong, irreversible modulation of olfactory responses that is consistent with effects seen in other neurons and sensory systems.
The document lists various ELISA kits for detecting human proteins and their specifications. It provides the product codes, product names, detection ranges, sensitivity thresholds, and test methods for 16 different ELISA kits. All kits use a sandwich method to detect proteins within specific concentration ranges, with sensitivities down to 0.09 ng/mL. The kits can measure levels of proteins involved in thyroid function, cancer, growth factors, and cell signaling.
Dr. Carol Treasure discusses adapting the KeratinoSens skin sensitization test to be animal-product free. She explains that most in vitro testing methods still use animal components like fetal bovine serum and outlines the challenges of replacing them. XCellR8 adapted KeratinoSens to use human serum and recombinant trypsin instead. They validated the modified method using reference chemicals and it correctly classified all chemicals. The adapted method was published as an OECD test guideline annex, demonstrating regulatory acceptance of animal-product free in vitro testing.
Elisa kits for infantile hepatitis syndrome researchElabscience
Infantile hepatitis syndrome refers to the neonatal period with symptoms of intrahepatic obstructive jaundice, liver and spleen enlargement, abnormal liver function. Because the cause is more, the exact reasons of each case is difficult to determine as a result of complex pathogenys. More info visit: http://www.elabscience.com
18 elisa kits for mediastinal tumor researchElabscience
The mediastinum is the cavity that separates the lungs from the rest of the chest. It contains many important organs, such as heart, esophagus, trachea, thymus, aorta, fat, nerve and lymphatic tissue. Mediastinal tumor refers to tumors caused by abnormal developmental or acquired cyst.More info visit: http://www.elabscience.com
02.elisa kits for renal failure researchElabscience
Kidney failure, also known as renal failure or renal insufficiency, is a medical condition of impaired kidney function in which the kidneys fail to adequately filter metabolic wastes from the blood. The two main forms are acute kidney injury, which is often reversible with adequate treatment, and chronic kidney disease, which is often not reversible. In both cases, there is usually an underlying cause.More info visit: http://www.elabscience.com
24 elisa kits for carcinoma of penis researchElabscience
Carcinoma of penis, also called penile cancer, is a malignant growth found on the skin or in the tissues of the penis. Around 95% of penile cancers are squamous cell carcinomas. Other types of penile cancer such as Merkel cell carcinoma, small cell carcinoma, melanoma and other are generally rare.Symptoms include redness of the penis, rash on the penis, foul smelling discharge from the penis and pain in the penis, etc. More info visit: http://www.elabscience.com
Edema (also oedema, dropsy, and hydropsy) is an abnormal accumulation of fluid in the interstitium, located beneath the skin and in the cavities of the body, which can cause severe pain. Clinically, edema manifests as swelling. More info visit: http://www.elabscience.com
07.elisa kits for sick sinus syndrome researchElabscience
Sick sinus syndrome(SSS), also called sinus dysfunction, or sinoatrial node disease ("SND"), is a group of abnormal heart rhythms (arrhythmias) presumably caused by a malfunction of the sinus node, the heart's primary pacemaker.More info visit: http://www.elabscience.com
Elisa kits for children abdominal pain researchElabscience
Children abdominal pain(also called infantile abdominal pain) is one of the most common symptoms in childhood. Abdominal pain, also known as stomach pain, is a common symptom associated with both temporary, non-serious disorders and more serious conditions.The reasons that cause abdominal pain vary widely, involving almost all kinds of diseases.Common causes of pain in the abdomen include gastroenteritis and irritable bowel syndrome.More info visit: http://www.elabscience.com
09 elisa kits for hemangioma of bone researchElabscience
Hemagioma can occurs in all parts of the body, and it may also occurs
in bone, but the latter is more rare than the others parts of body.
Hemangioma of bone sometimes exists with hemangioma of other parts at
the same time, it is a common tumor of benign tumor attached in bone
tissue.More info visit: http://www.elabscience.com
01 elisa kits for malignant ascites researchElabscience
The document lists various ELISA kits for detecting levels of proteins involved in cancer. For each kit, it provides the product code, protein name being detected, detection range, sensitivity, and test method. The kits use ELISA (enzyme-linked immunosorbent assay) to detect proteins including TP53, CDKN2A, KRAS, EGFR, CCND1, VEGF-A, IL-6, PDGFRβ, ALB, BRAF, JAK2, ERα, MMP-2, BRCA2, TM, MMP-9, and BRCA1 within certain concentration ranges with good sensitivity. All kits employ a sandwich ELISA test method.
04..elisa kits for peyronie disease researchElabscience
Prostatitis is inflammation of the prostate gland. Prostatitis is classified into acute, chronic, asymptomatic inflammatory prostatitis, and chronic pelvic pain syndrome. Acute prostatitis is a bacterial infection of the prostate gland that requires urgent medical treatment. Chronic prostatitis is a pelvic paincondition in men, formerly known as prostatodynia (painful prostate).More info visit: http://www.elabscience.com
Elisa kits for children juvenile bronchial asthma researchElabscience
ELISA Kits for Children Bronchial Asthma
Children bronchial asthma is a common disease of respiration tract in children. Bronchial (asthma for short) is a common long-term inflammatory disease of the airways of the lungs. Symptoms include episodes of wheezing, coughing, chest tightness, and shortness of breath. These episodes may occur a few times a day or a few times per week.More info visit:
http://www.elabscience.com
Elisa kits for children nephrotic syndrome researchElabscience
Children nephrotic syndrome refers to nephrotic syndrome occurred in children, which is a syndrome comprising signs of nephrosis, chiefly proteinuria, hypoalbuminemia, and edema. It is a component of glomerulonephrosis, in which different degrees of proteinuria occur. Essentially, loss of protein through the kidneys (proteinuria) leads to low protein levels in the blood (hypoproteinemia including hypoalbuminemia), which causes water to be drawn into soft tissues (edema).
01.elisa kits for urethral syndrome researchElabscience
Urethral syndrome is characterised by a set of symptoms typically associated with lower urinary tract infection, such as painful urination (dysuria) and frequency. It is a diagnosis of exclusion, made when there is no significant presence of bacteriuria with a conventional pathogen ruling out urinary tract infection, and when cystoscopy shows no inflammation of the bladder, ruling out interstitial cystitis and cystitis cystica.More info visit: http://www.elabscience.com
Elisa kits for congenital heart disease researchElabscience
Congenital heart defect (CHD), also known as a congenital heart anomaly or congenital heart
disease, is a problem in the structure of the heart that is present at birth.Signs and
symptoms depend on the specific type of problem. Symptoms can vary from none to life-
threatening.The cause of a congenital heart defect is often unknown.Most congenital heart
problems do not occur with other diseases. Complications that can result from heart defects
include heart failure.More info visit: http://www.elabscience.com
09.elisa kits for rheumatic heart disease researchElabscience
Rheumatic heart disease refers to the valvular heart disease resulting from rheumativ fever. Rheumatic fever is an inflammatory disease that can involve the heart, joints, skin and brain. The damaged valves may result in heart failure, atrial fibrillation and infection of the valves.More info visit: http://www.elabscience.com
SABiosciences produces ELISArray kits that allow researchers to simultaneously profile multiple cytokines or chemokines from a single sample in a single plate. This overcomes limitations of traditional ELISA kits that profile only one cytokine at a time, saving researchers both time and money. The ELISArray kits have been optimized to provide high performance and sensitivity comparable to individual ELISA kits while being easy to use with minimal hands-on time. They allow researchers to initially screen samples to see changes in cytokine expression over time or conditions before following up with individual kits for quantitative results. The document provides examples of how ELISArray kits have been used successfully to profile cytokine induction in human immune cells.
The document describes the activities of Biopra, a contract research organization serving the drug discovery industry. Biopra was founded by three experts in Alpha technologies who previously worked at PerkinElmer. Biopra offers assay development services focusing on Alpha technologies like AlphaScreen and AlphaLISA. Their services include developing biochemical and cellular assays for targets like kinases, epigenetic enzymes, and biomarkers. They have expertise in areas like epigenetics, biomarker detection, protein interactions, and kinase profiling. Biopra aims to provide high quality services executed by technology pioneers with quick turnaround times through a collaborative approach.
Heat stress affects the kinetics of olfactory responses in rats. The study found that exposing rats to high temperatures of 45°C for 25 minutes significantly increased their body temperatures and caused faster rise and decay times of electrical responses in the olfactory epithelium to odorants. Heat stress also increased blood levels of lipopolysaccharides and free vesicles but did not change most cytokine levels, except for an increase in IL-10. This suggests heat stress causes strong, irreversible modulation of olfactory responses that is consistent with effects seen in other neurons and sensory systems.
The document lists various ELISA kits for detecting human proteins and their specifications. It provides the product codes, product names, detection ranges, sensitivity thresholds, and test methods for 16 different ELISA kits. All kits use a sandwich method to detect proteins within specific concentration ranges, with sensitivities down to 0.09 ng/mL. The kits can measure levels of proteins involved in thyroid function, cancer, growth factors, and cell signaling.
Dr. Carol Treasure discusses adapting the KeratinoSens skin sensitization test to be animal-product free. She explains that most in vitro testing methods still use animal components like fetal bovine serum and outlines the challenges of replacing them. XCellR8 adapted KeratinoSens to use human serum and recombinant trypsin instead. They validated the modified method using reference chemicals and it correctly classified all chemicals. The adapted method was published as an OECD test guideline annex, demonstrating regulatory acceptance of animal-product free in vitro testing.
Elisa kits for infantile hepatitis syndrome researchElabscience
Infantile hepatitis syndrome refers to the neonatal period with symptoms of intrahepatic obstructive jaundice, liver and spleen enlargement, abnormal liver function. Because the cause is more, the exact reasons of each case is difficult to determine as a result of complex pathogenys. More info visit: http://www.elabscience.com
18 elisa kits for mediastinal tumor researchElabscience
The mediastinum is the cavity that separates the lungs from the rest of the chest. It contains many important organs, such as heart, esophagus, trachea, thymus, aorta, fat, nerve and lymphatic tissue. Mediastinal tumor refers to tumors caused by abnormal developmental or acquired cyst.More info visit: http://www.elabscience.com
02.elisa kits for renal failure researchElabscience
Kidney failure, also known as renal failure or renal insufficiency, is a medical condition of impaired kidney function in which the kidneys fail to adequately filter metabolic wastes from the blood. The two main forms are acute kidney injury, which is often reversible with adequate treatment, and chronic kidney disease, which is often not reversible. In both cases, there is usually an underlying cause.More info visit: http://www.elabscience.com
24 elisa kits for carcinoma of penis researchElabscience
Carcinoma of penis, also called penile cancer, is a malignant growth found on the skin or in the tissues of the penis. Around 95% of penile cancers are squamous cell carcinomas. Other types of penile cancer such as Merkel cell carcinoma, small cell carcinoma, melanoma and other are generally rare.Symptoms include redness of the penis, rash on the penis, foul smelling discharge from the penis and pain in the penis, etc. More info visit: http://www.elabscience.com
Edema (also oedema, dropsy, and hydropsy) is an abnormal accumulation of fluid in the interstitium, located beneath the skin and in the cavities of the body, which can cause severe pain. Clinically, edema manifests as swelling. More info visit: http://www.elabscience.com
07.elisa kits for sick sinus syndrome researchElabscience
Sick sinus syndrome(SSS), also called sinus dysfunction, or sinoatrial node disease ("SND"), is a group of abnormal heart rhythms (arrhythmias) presumably caused by a malfunction of the sinus node, the heart's primary pacemaker.More info visit: http://www.elabscience.com
Elisa kits for children abdominal pain researchElabscience
Children abdominal pain(also called infantile abdominal pain) is one of the most common symptoms in childhood. Abdominal pain, also known as stomach pain, is a common symptom associated with both temporary, non-serious disorders and more serious conditions.The reasons that cause abdominal pain vary widely, involving almost all kinds of diseases.Common causes of pain in the abdomen include gastroenteritis and irritable bowel syndrome.More info visit: http://www.elabscience.com
09 elisa kits for hemangioma of bone researchElabscience
Hemagioma can occurs in all parts of the body, and it may also occurs
in bone, but the latter is more rare than the others parts of body.
Hemangioma of bone sometimes exists with hemangioma of other parts at
the same time, it is a common tumor of benign tumor attached in bone
tissue.More info visit: http://www.elabscience.com
01 elisa kits for malignant ascites researchElabscience
The document lists various ELISA kits for detecting levels of proteins involved in cancer. For each kit, it provides the product code, protein name being detected, detection range, sensitivity, and test method. The kits use ELISA (enzyme-linked immunosorbent assay) to detect proteins including TP53, CDKN2A, KRAS, EGFR, CCND1, VEGF-A, IL-6, PDGFRβ, ALB, BRAF, JAK2, ERα, MMP-2, BRCA2, TM, MMP-9, and BRCA1 within certain concentration ranges with good sensitivity. All kits employ a sandwich ELISA test method.
04..elisa kits for peyronie disease researchElabscience
Prostatitis is inflammation of the prostate gland. Prostatitis is classified into acute, chronic, asymptomatic inflammatory prostatitis, and chronic pelvic pain syndrome. Acute prostatitis is a bacterial infection of the prostate gland that requires urgent medical treatment. Chronic prostatitis is a pelvic paincondition in men, formerly known as prostatodynia (painful prostate).More info visit: http://www.elabscience.com
Elisa kits for children juvenile bronchial asthma researchElabscience
ELISA Kits for Children Bronchial Asthma
Children bronchial asthma is a common disease of respiration tract in children. Bronchial (asthma for short) is a common long-term inflammatory disease of the airways of the lungs. Symptoms include episodes of wheezing, coughing, chest tightness, and shortness of breath. These episodes may occur a few times a day or a few times per week.More info visit:
http://www.elabscience.com
Elisa kits for children nephrotic syndrome researchElabscience
Children nephrotic syndrome refers to nephrotic syndrome occurred in children, which is a syndrome comprising signs of nephrosis, chiefly proteinuria, hypoalbuminemia, and edema. It is a component of glomerulonephrosis, in which different degrees of proteinuria occur. Essentially, loss of protein through the kidneys (proteinuria) leads to low protein levels in the blood (hypoproteinemia including hypoalbuminemia), which causes water to be drawn into soft tissues (edema).
01.elisa kits for urethral syndrome researchElabscience
Urethral syndrome is characterised by a set of symptoms typically associated with lower urinary tract infection, such as painful urination (dysuria) and frequency. It is a diagnosis of exclusion, made when there is no significant presence of bacteriuria with a conventional pathogen ruling out urinary tract infection, and when cystoscopy shows no inflammation of the bladder, ruling out interstitial cystitis and cystitis cystica.More info visit: http://www.elabscience.com
Elisa kits for congenital heart disease researchElabscience
Congenital heart defect (CHD), also known as a congenital heart anomaly or congenital heart
disease, is a problem in the structure of the heart that is present at birth.Signs and
symptoms depend on the specific type of problem. Symptoms can vary from none to life-
threatening.The cause of a congenital heart defect is often unknown.Most congenital heart
problems do not occur with other diseases. Complications that can result from heart defects
include heart failure.More info visit: http://www.elabscience.com
09.elisa kits for rheumatic heart disease researchElabscience
Rheumatic heart disease refers to the valvular heart disease resulting from rheumativ fever. Rheumatic fever is an inflammatory disease that can involve the heart, joints, skin and brain. The damaged valves may result in heart failure, atrial fibrillation and infection of the valves.More info visit: http://www.elabscience.com
SABiosciences produces ELISArray kits that allow researchers to simultaneously profile multiple cytokines or chemokines from a single sample in a single plate. This overcomes limitations of traditional ELISA kits that profile only one cytokine at a time, saving researchers both time and money. The ELISArray kits have been optimized to provide high performance and sensitivity comparable to individual ELISA kits while being easy to use with minimal hands-on time. They allow researchers to initially screen samples to see changes in cytokine expression over time or conditions before following up with individual kits for quantitative results. The document provides examples of how ELISArray kits have been used successfully to profile cytokine induction in human immune cells.
The document describes the activities of Biopra, a contract research organization serving the drug discovery industry. Biopra was founded by three experts in Alpha technologies who previously worked at PerkinElmer. Biopra offers assay development services focusing on Alpha technologies like AlphaScreen and AlphaLISA. Their services include developing biochemical and cellular assays for targets like kinases, epigenetic enzymes, and biomarkers. They have expertise in areas like epigenetics, biomarker detection, protein interactions, and kinase profiling. Biopra aims to provide high quality services executed by technology pioneers with quick turnaround times through a collaborative approach.
This document discusses biomarkers for assessing immune function throughout the drug development process. It describes how various techniques can be used to identify, validate, and qualify biomarkers. These include flow cytometry to analyze cell populations and activation markers, Luminex to measure cytokine levels, and gene expression profiling using NanoString. Whole blood stimulation assays are discussed as a way to assess target engagement and immune responses ex vivo. The importance of assay validation and understanding sources of variation are also covered. Biomarkers can provide insights into mechanisms of action, safety, and efficacy to support clinical development.
This document summarizes a study evaluating the utility of using multiplex panels of biomarkers for screening purposes without prior knowledge of biomarkers of interest. Fifteen multiplex panels were developed containing up to ten assays each, requiring less than 1 mL of sample to measure 122 biomarkers total. Assays showed low cross-reactivity, broad dynamic ranges, and good reproducibility. The multiplex panels allowed rapid screening and stratification of patient populations to identify potential biomarker targets.
Profile Multiple Cytokines and Chemokines Simultaneously with Very High Sensi...QIAGEN
Learn how to profile multiple cytokines and chemokines simultaneously with very high sensitivity and specificity using the standard ELISA reader. Available in different formats to suit your research needs such as single-analyte, multi-analyte or custom mix-n-match format for human, mouse and rat.
ACCULAB Corporation is a manufacturer and supplier of laboratory and medical instruments based in New York. It incorporates 7 companies to offer a wide range of products at different price points to meet customer demands. ACCULAB aims to provide both high quality products and competitive prices with a vision of being a one-stop shop for all laboratory equipment needs through excellent customer service.
The document describes Cignal Reporter Assays from SABiosciences that enable simple and robust analysis of signal transduction pathways. The assays utilize dual-luciferase reporters containing optimized transcriptional regulatory elements and luciferase variants to provide high sensitivity and low variability. The assays allow monitoring of 29 pathways and are available in different formats for various cell types and applications like RNAi and small molecule screening.
The document discusses analyzing cytokines and chemokines simultaneously using ELISArray kits. It describes the challenges of analyzing cytokines one at a time using traditional ELISA methods. ELISArray kits allow measuring multiple cytokines from a single sample using a simple ELISA protocol. They provide flexibility in selecting the cytokines analyzed and have been optimized to have consistent performance across all cytokines measured. An example application demonstrates their use in profiling cytokine production over time in stimulated immune cells.
The document discusses various topics related to molecular profiling and personalized medicine. It describes first generation molecular profiling techniques like gene sequencing, microarrays, and PCR. It then covers next generation sequencing technologies like Roche 454, Illumina, and ABI SOLID. It also discusses second generation techniques for DNA and RNA profiling including exome sequencing, ChIP-seq, and RNA-seq. Finally, it briefly mentions third generation sequencing and epigenetic profiling.
This document provides information about the services offered by a central laboratory for clinical trials and drug discovery. The laboratory provides quality central laboratory services for clinical trials, maintains confidentiality, and meets regulatory standards. It has offered services to leading pharmaceutical companies for preclinical and clinical trials. The services described include in vitro and in vivo ADME studies, cell-based assays, in vivo pharmacology studies, toxicology studies, analytical chemistry services, and more.
Answer four fundamental questions on how to develop the most innovative cancer immunotherapy treatments, starting with screening for lead molecules and ending with evaluation of combination therapies.
Toxikon is a contract research organization providing analytical, preclinical, and toxicology services. It has over 30 years of experience working with medical device, pharmaceutical, and biotech clients. The company has laboratories in the US and Belgium that are ISO 17025 accredited and GMP/GLP compliant. It offers a range of proof-of-concept, preclinical toxicology, and analytical chemistry services to support drug and device development from research through regulatory approval.
1. The document discusses the use of nucleic acid testing (NAT) to screen blood donations for HIV, HCV, and HBV. NAT helps reduce the window period by directly detecting viral nucleic acids, providing an additional layer of safety beyond serological testing alone.
2. Data from testing over 40,000 blood donations in India showed a NAT yield rate of 1 in 598 donations, detecting 68 donations that were infectious by NAT but non-reactive by serology.
3. Studies comparing different NAT assays found that the Ultrio Plus assay had greater sensitivity than older assays, detecting more true positive donations during the window period.
This document discusses clinical and consumer applications of microarrays and genotyping technologies. It provides an overview of genotyping and different technologies like PCR microarrays and SNP microarrays. It describes how microarrays are still useful despite the rise of sequencing due to their low cost, high throughput, and ability to test millions of markers. The document outlines several applications of microarrays like direct-to-consumer testing, pharmacogenetics, and clinical sequencing. It also discusses challenges and trends in these areas like global initiatives to increase genomic data sharing.
A rapid library preparation method with custom assay designs for detection of...Thermo Fisher Scientific
Herein, we describe a new research method for library
preparation using the Ion AmpliSeq™ HD Library Kit with
custom assay designs from Ion AmpliSeq HD Panels for
detection of low level variants from liquid biopsy samples. This
method includes incorporation of molecular tags that enable
0.1% Limit of Detection (LOD) in cell free DNA (cfDNA) and
dual barcodes for sample identification. This method is also
applicable to formalin-fixed paraffin embedded (FFPE)
samples. The libraries can be prepared in as little as 3 hours
and are compatible for analysis with the Ion GeneStudio™ S5
system
ELISA use an enzyme to detect the binding of antigen (Ag) antibody (Ab). • The enzyme converts a colorless substrate (chromogen) to a colored product, indicating the presence of Ag:Ab binding. • An ELISA can be used to detect either the presence of antigens or antibodies in a sample depending how the test is designed
This document discusses food authenticity testing and the issues with current testing methods. It introduces next generation sequencing (NGS) as a new method for food authenticity testing. NGS allows for the simultaneous detection of thousands of potential food contaminants in a single test, overcoming limitations of current polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) methods. NGS involves amplifying DNA from all species in a mixed sample, sequencing the amplified products, and comparing the sequences to a reference database to identify contaminants. While a major improvement, NGS also has limitations, such as an inability to currently quantify contamination levels.
Cosa hanno in comune un mattoncino Lego e la backdoor XZ?Speck&Tech
ABSTRACT: A prima vista, un mattoncino Lego e la backdoor XZ potrebbero avere in comune il fatto di essere entrambi blocchi di costruzione, o dipendenze di progetti creativi e software. La realtà è che un mattoncino Lego e il caso della backdoor XZ hanno molto di più di tutto ciò in comune.
Partecipate alla presentazione per immergervi in una storia di interoperabilità, standard e formati aperti, per poi discutere del ruolo importante che i contributori hanno in una comunità open source sostenibile.
BIO: Sostenitrice del software libero e dei formati standard e aperti. È stata un membro attivo dei progetti Fedora e openSUSE e ha co-fondato l'Associazione LibreItalia dove è stata coinvolta in diversi eventi, migrazioni e formazione relativi a LibreOffice. In precedenza ha lavorato a migrazioni e corsi di formazione su LibreOffice per diverse amministrazioni pubbliche e privati. Da gennaio 2020 lavora in SUSE come Software Release Engineer per Uyuni e SUSE Manager e quando non segue la sua passione per i computer e per Geeko coltiva la sua curiosità per l'astronomia (da cui deriva il suo nickname deneb_alpha).
Programming Foundation Models with DSPy - Meetup SlidesZilliz
Prompting language models is hard, while programming language models is easy. In this talk, I will discuss the state-of-the-art framework DSPy for programming foundation models with its powerful optimizers and runtime constraint system.
Climate Impact of Software Testing at Nordic Testing DaysKari Kakkonen
My slides at Nordic Testing Days 6.6.2024
Climate impact / sustainability of software testing discussed on the talk. ICT and testing must carry their part of global responsibility to help with the climat warming. We can minimize the carbon footprint but we can also have a carbon handprint, a positive impact on the climate. Quality characteristics can be added with sustainability, and then measured continuously. Test environments can be used less, and in smaller scale and on demand. Test techniques can be used in optimizing or minimizing number of tests. Test automation can be used to speed up testing.
“An Outlook of the Ongoing and Future Relationship between Blockchain Technologies and Process-aware Information Systems.” Invited talk at the joint workshop on Blockchain for Information Systems (BC4IS) and Blockchain for Trusted Data Sharing (B4TDS), co-located with with the 36th International Conference on Advanced Information Systems Engineering (CAiSE), 3 June 2024, Limassol, Cyprus.
Full-RAG: A modern architecture for hyper-personalizationZilliz
Mike Del Balso, CEO & Co-Founder at Tecton, presents "Full RAG," a novel approach to AI recommendation systems, aiming to push beyond the limitations of traditional models through a deep integration of contextual insights and real-time data, leveraging the Retrieval-Augmented Generation architecture. This talk will outline Full RAG's potential to significantly enhance personalization, address engineering challenges such as data management and model training, and introduce data enrichment with reranking as a key solution. Attendees will gain crucial insights into the importance of hyperpersonalization in AI, the capabilities of Full RAG for advanced personalization, and strategies for managing complex data integrations for deploying cutting-edge AI solutions.
Things to Consider When Choosing a Website Developer for your Website | FODUUFODUU
Choosing the right website developer is crucial for your business. This article covers essential factors to consider, including experience, portfolio, technical skills, communication, pricing, reputation & reviews, cost and budget considerations and post-launch support. Make an informed decision to ensure your website meets your business goals.
Best 20 SEO Techniques To Improve Website Visibility In SERPPixlogix Infotech
Boost your website's visibility with proven SEO techniques! Our latest blog dives into essential strategies to enhance your online presence, increase traffic, and rank higher on search engines. From keyword optimization to quality content creation, learn how to make your site stand out in the crowded digital landscape. Discover actionable tips and expert insights to elevate your SEO game.
For the full video of this presentation, please visit: https://www.edge-ai-vision.com/2024/06/building-and-scaling-ai-applications-with-the-nx-ai-manager-a-presentation-from-network-optix/
Robin van Emden, Senior Director of Data Science at Network Optix, presents the “Building and Scaling AI Applications with the Nx AI Manager,” tutorial at the May 2024 Embedded Vision Summit.
In this presentation, van Emden covers the basics of scaling edge AI solutions using the Nx tool kit. He emphasizes the process of developing AI models and deploying them globally. He also showcases the conversion of AI models and the creation of effective edge AI pipelines, with a focus on pre-processing, model conversion, selecting the appropriate inference engine for the target hardware and post-processing.
van Emden shows how Nx can simplify the developer’s life and facilitate a rapid transition from concept to production-ready applications.He provides valuable insights into developing scalable and efficient edge AI solutions, with a strong focus on practical implementation.
Threats to mobile devices are more prevalent and increasing in scope and complexity. Users of mobile devices desire to take full advantage of the features
available on those devices, but many of the features provide convenience and capability but sacrifice security. This best practices guide outlines steps the users can take to better protect personal devices and information.
Infrastructure Challenges in Scaling RAG with Custom AI modelsZilliz
Building Retrieval-Augmented Generation (RAG) systems with open-source and custom AI models is a complex task. This talk explores the challenges in productionizing RAG systems, including retrieval performance, response synthesis, and evaluation. We’ll discuss how to leverage open-source models like text embeddings, language models, and custom fine-tuned models to enhance RAG performance. Additionally, we’ll cover how BentoML can help orchestrate and scale these AI components efficiently, ensuring seamless deployment and management of RAG systems in the cloud.
HCL Notes and Domino License Cost Reduction in the World of DLAUpanagenda
Webinar Recording: https://www.panagenda.com/webinars/hcl-notes-and-domino-license-cost-reduction-in-the-world-of-dlau/
The introduction of DLAU and the CCB & CCX licensing model caused quite a stir in the HCL community. As a Notes and Domino customer, you may have faced challenges with unexpected user counts and license costs. You probably have questions on how this new licensing approach works and how to benefit from it. Most importantly, you likely have budget constraints and want to save money where possible. Don’t worry, we can help with all of this!
We’ll show you how to fix common misconfigurations that cause higher-than-expected user counts, and how to identify accounts which you can deactivate to save money. There are also frequent patterns that can cause unnecessary cost, like using a person document instead of a mail-in for shared mailboxes. We’ll provide examples and solutions for those as well. And naturally we’ll explain the new licensing model.
Join HCL Ambassador Marc Thomas in this webinar with a special guest appearance from Franz Walder. It will give you the tools and know-how to stay on top of what is going on with Domino licensing. You will be able lower your cost through an optimized configuration and keep it low going forward.
These topics will be covered
- Reducing license cost by finding and fixing misconfigurations and superfluous accounts
- How do CCB and CCX licenses really work?
- Understanding the DLAU tool and how to best utilize it
- Tips for common problem areas, like team mailboxes, functional/test users, etc
- Practical examples and best practices to implement right away
Have you ever been confused by the myriad of choices offered by AWS for hosting a website or an API?
Lambda, Elastic Beanstalk, Lightsail, Amplify, S3 (and more!) can each host websites + APIs. But which one should we choose?
Which one is cheapest? Which one is fastest? Which one will scale to meet our needs?
Join me in this session as we dive into each AWS hosting service to determine which one is best for your scenario and explain why!
Unlock the Future of Search with MongoDB Atlas_ Vector Search Unleashed.pdfMalak Abu Hammad
Discover how MongoDB Atlas and vector search technology can revolutionize your application's search capabilities. This comprehensive presentation covers:
* What is Vector Search?
* Importance and benefits of vector search
* Practical use cases across various industries
* Step-by-step implementation guide
* Live demos with code snippets
* Enhancing LLM capabilities with vector search
* Best practices and optimization strategies
Perfect for developers, AI enthusiasts, and tech leaders. Learn how to leverage MongoDB Atlas to deliver highly relevant, context-aware search results, transforming your data retrieval process. Stay ahead in tech innovation and maximize the potential of your applications.
#MongoDB #VectorSearch #AI #SemanticSearch #TechInnovation #DataScience #LLM #MachineLearning #SearchTechnology
UiPath Test Automation using UiPath Test Suite series, part 6DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 6. In this session, we will cover Test Automation with generative AI and Open AI.
UiPath Test Automation with generative AI and Open AI webinar offers an in-depth exploration of leveraging cutting-edge technologies for test automation within the UiPath platform. Attendees will delve into the integration of generative AI, a test automation solution, with Open AI advanced natural language processing capabilities.
Throughout the session, participants will discover how this synergy empowers testers to automate repetitive tasks, enhance testing accuracy, and expedite the software testing life cycle. Topics covered include the seamless integration process, practical use cases, and the benefits of harnessing AI-driven automation for UiPath testing initiatives. By attending this webinar, testers, and automation professionals can gain valuable insights into harnessing the power of AI to optimize their test automation workflows within the UiPath ecosystem, ultimately driving efficiency and quality in software development processes.
What will you get from this session?
1. Insights into integrating generative AI.
2. Understanding how this integration enhances test automation within the UiPath platform
3. Practical demonstrations
4. Exploration of real-world use cases illustrating the benefits of AI-driven test automation for UiPath
Topics covered:
What is generative AI
Test Automation with generative AI and Open AI.
UiPath integration with generative AI
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Driving Business Innovation: Latest Generative AI Advancements & Success StorySafe Software
Are you ready to revolutionize how you handle data? Join us for a webinar where we’ll bring you up to speed with the latest advancements in Generative AI technology and discover how leveraging FME with tools from giants like Google Gemini, Amazon, and Microsoft OpenAI can supercharge your workflow efficiency.
During the hour, we’ll take you through:
Guest Speaker Segment with Hannah Barrington: Dive into the world of dynamic real estate marketing with Hannah, the Marketing Manager at Workspace Group. Hear firsthand how their team generates engaging descriptions for thousands of office units by integrating diverse data sources—from PDF floorplans to web pages—using FME transformers, like OpenAIVisionConnector and AnthropicVisionConnector. This use case will show you how GenAI can streamline content creation for marketing across the board.
Ollama Use Case: Learn how Scenario Specialist Dmitri Bagh has utilized Ollama within FME to input data, create custom models, and enhance security protocols. This segment will include demos to illustrate the full capabilities of FME in AI-driven processes.
Custom AI Models: Discover how to leverage FME to build personalized AI models using your data. Whether it’s populating a model with local data for added security or integrating public AI tools, find out how FME facilitates a versatile and secure approach to AI.
We’ll wrap up with a live Q&A session where you can engage with our experts on your specific use cases, and learn more about optimizing your data workflows with AI.
This webinar is ideal for professionals seeking to harness the power of AI within their data management systems while ensuring high levels of customization and security. Whether you're a novice or an expert, gain actionable insights and strategies to elevate your data processes. Join us to see how FME and AI can revolutionize how you work with data!
Monitoring and Managing Anomaly Detection on OpenShift.pdfTosin Akinosho
Monitoring and Managing Anomaly Detection on OpenShift
Overview
Dive into the world of anomaly detection on edge devices with our comprehensive hands-on tutorial. This SlideShare presentation will guide you through the entire process, from data collection and model training to edge deployment and real-time monitoring. Perfect for those looking to implement robust anomaly detection systems on resource-constrained IoT/edge devices.
Key Topics Covered
1. Introduction to Anomaly Detection
- Understand the fundamentals of anomaly detection and its importance in identifying unusual behavior or failures in systems.
2. Understanding Edge (IoT)
- Learn about edge computing and IoT, and how they enable real-time data processing and decision-making at the source.
3. What is ArgoCD?
- Discover ArgoCD, a declarative, GitOps continuous delivery tool for Kubernetes, and its role in deploying applications on edge devices.
4. Deployment Using ArgoCD for Edge Devices
- Step-by-step guide on deploying anomaly detection models on edge devices using ArgoCD.
5. Introduction to Apache Kafka and S3
- Explore Apache Kafka for real-time data streaming and Amazon S3 for scalable storage solutions.
6. Viewing Kafka Messages in the Data Lake
- Learn how to view and analyze Kafka messages stored in a data lake for better insights.
7. What is Prometheus?
- Get to know Prometheus, an open-source monitoring and alerting toolkit, and its application in monitoring edge devices.
8. Monitoring Application Metrics with Prometheus
- Detailed instructions on setting up Prometheus to monitor the performance and health of your anomaly detection system.
9. What is Camel K?
- Introduction to Camel K, a lightweight integration framework built on Apache Camel, designed for Kubernetes.
10. Configuring Camel K Integrations for Data Pipelines
- Learn how to configure Camel K for seamless data pipeline integrations in your anomaly detection workflow.
11. What is a Jupyter Notebook?
- Overview of Jupyter Notebooks, an open-source web application for creating and sharing documents with live code, equations, visualizations, and narrative text.
12. Jupyter Notebooks with Code Examples
- Hands-on examples and code snippets in Jupyter Notebooks to help you implement and test anomaly detection models.
Essentials of Automations: The Art of Triggers and Actions in FMESafe Software
In this second installment of our Essentials of Automations webinar series, we’ll explore the landscape of triggers and actions, guiding you through the nuances of authoring and adapting workspaces for seamless automations. Gain an understanding of the full spectrum of triggers and actions available in FME, empowering you to enhance your workspaces for efficient automation.
We’ll kick things off by showcasing the most commonly used event-based triggers, introducing you to various automation workflows like manual triggers, schedules, directory watchers, and more. Plus, see how these elements play out in real scenarios.
Whether you’re tweaking your current setup or building from the ground up, this session will arm you with the tools and insights needed to transform your FME usage into a powerhouse of productivity. Join us to discover effective strategies that simplify complex processes, enhancing your productivity and transforming your data management practices with FME. Let’s turn complexity into clarity and make your workspaces work wonders!
TrustArc Webinar - 2024 Global Privacy SurveyTrustArc
How does your privacy program stack up against your peers? What challenges are privacy teams tackling and prioritizing in 2024?
In the fifth annual Global Privacy Benchmarks Survey, we asked over 1,800 global privacy professionals and business executives to share their perspectives on the current state of privacy inside and outside of their organizations. This year’s report focused on emerging areas of importance for privacy and compliance professionals, including considerations and implications of Artificial Intelligence (AI) technologies, building brand trust, and different approaches for achieving higher privacy competence scores.
See how organizational priorities and strategic approaches to data security and privacy are evolving around the globe.
This webinar will review:
- The top 10 privacy insights from the fifth annual Global Privacy Benchmarks Survey
- The top challenges for privacy leaders, practitioners, and organizations in 2024
- Key themes to consider in developing and maintaining your privacy program
1. “For over 24 years, we’ve worked hand in hand with industry,
academic, and government researchers to help solve difficult
assay development and protein quantification problems.”
Stephen Parent, PhD
Director of Business Development
sparent@pblbio.com
(732) 777-9123 x117
2. PBL Assay Science History
• Founded in 1990 by Dr. Sidney Pestka
• 35 employees based in Piscataway, NJ
• World’s largest producer of interferons and related
cytokine products for the research market
– ELISA and multiplex assay kits
– Recombinant proteins
– Antibodies
– Assay services
– Custom services
3. High Quality Assay Solutions Interrogating
Interferon and Cytokine Biology
4. Assay Solutions Interrogating IFN and Cytokine Biology
• Off the Shelf Assays
– More than 70 Interferon ELISAs, antibodies and cell-
based assays
• Assay Services Offerings
– Sample Testing and Screening
– More than 40 Singulex Erenna biomarker assays
– Assay Design and Development
– Custom Services
– We provide ~ 70 different services
5. Assay Services Capabilities
Cell-Based Assay Development
Antiviral Assays
Cytokine/Chemokine Bioactivity Assays
Immunoassays
Development
Single Analyte & Multiplex ELISAs
Ultrasensitive Cytokine Detection Assays
using Singulex Erenna® technology
More than 40 Assays Available
Human and Mouse
Reagent Development & Characterization
Assay Optimization
Technology Transfer
Consulting in Assay Development and
Therapeutics Development
6. IL-17
Ultrasensitive Cytokine Detection Service
using Singulex Erenna® Immunoassay System
Modified IA process
using magnetic beads
Single Molecule
Detection (SMD)
technology
50- 1000 fold higher sensitivity over existing IA
products
More than 40 Assays Available
7. IL-17
Singulex Single Molecule Counting (SMC)
Erenna®
4.
5.
► Elution buffer
dissociates the
complex (analyte +
detection antibody
in simple buffer)
► Transfer to 384-
well plate
► Draw eluate into the
instrument for reading
► Fluorescently labeled
antibodies introduced
to laser confocal
microscope digital
counter
► Molecules are
individually detected
and digitized
Elution Molecule counting
Proprietary Singulex technology
Sample collection
2.
3.
► Transfer samples
to 96-well plate
► Incubate with
capture magnetic
antibody-coated
beads
► Wash to remove
unbound
antibodies
► Incubate with dye-
labeled detection
antibodies
1.
► Low abundance of
certain biomarkers in
complex biological
samples
Antibody incubation Washing & fluorescent labeling
Standard immunoassay protocol
More than 40 Assays Available
9. Examples of Available Cell-based IFN Assays
Cytopathic Effect (CPE) Inhibition Assay
Neutralization of 1 U of IFN in
cytopathic effect (CPE) inhibition assay
A549 human lung cancer cell line with
encephalomyocarditis virus (EMCV)
Cell-based Neutralizing Antibody Assay
10. Examples of Cell Proliferation & Anti-Proliferation Assays
OVCAR-3 human adenocarcinoma line TF-1 human erythroleukemia line
Proliferation quantified using soluble tetrazolium reagent
11. Immunoassay Products
• 10 Human Interferon ELISAs
• 3 Mouse Interferon ELISAs
• 1 Cynomolgus/Rhesus Interferon ELISA
• 1 Human soluble IFNAR2 ELISA
• 2 Multiplex ELISAs quantifying Type I, II and III IFNs
and other cytokines
• Custom Multiplex ELISAs are also possible
12. You can have confidence in PBL ELISAs!
• Human IFN alpha ELISA study (2008)
• Split samples run at a CRO and PBL
• Slope = 0.991
• Correlation coefficient = 0.9931
• Human IFN beta ELISA study (2009)
• Split samples run at a CRO and PBL
• Slope = 1.036
• Correlation coefficient = 0.9966
• Human IFN beta ELISA study (2014)
• P. Conliffee et al. AAPS Biotechnology Conference
• Validated analytical range 2.34 - 150 pg/ml
• Dilutional linearity up to 1 : 2187
• Interassay precision (CV) ≤13.1%
• LLOQ 2.34 pg/ml
• No IFN-b detected in normal sera.
• Median quantifiable IFN-b was 17.3 pg/ml in MS sera (n=47), with 26
quantifiable & 21 below LLOQ.
• MS samples across different sites showed excellent correlation (r2=0.9975)
13. VeriKine Human Interferon ELISA Kits
• Human IFNa Multisubtype Serum Kit
• Validated at numerous sites
• Kit standard calibrated to NIH human IFN alpha
standard
• Dual detection range
– 12.5 – 1000 pg/ml
• LLOD: 1.6 pg/ml; LLOQ: 5.7 pg/ml
• Intra-assay, Inter-assay, and Inter-lot CV < 8%
• Compatible with 50% normal human serum, 50%
plasma, TCM and nasal lavage
• Unique broad subtype cross reactivity
• Detects Intron-A®, Roferon®, pegylated interferon alphas
• Human Interferon Beta Serum Kit
• Developed to meet ICH guidelines on validation of
analytical procedures
• Validated at 3 independent labs to date
• Kit standard calibrated to NIH human IFN beta
standard (correlates with NIBSC standard)
• The most sensitive human IFN beta ELISA in the
market
• Detection range: 1.2 - 150 pg/ml
• LLOD: 0.3 pg/ml; LLOQ: ~2 pg/ml
• Intra-assay, Inter-assay, and Inter-lot CV < 8%
• Compatible with normal human serum, plasma,
tissue culture media
• Characterized on MS patient sera
• Detects Betaseron®, Avonex®, Cynomolgus,
Rhesus IFN beta
• Detects IFN beta bound to soluble IFNAR2
receptors
14. VeriKine-HS High Sensitivity
Human Interferon Beta Serum ELISA Kit
90% of Patients on IFN
Beta therapy have
quantifiable IFN Beta
2% of Healthy Donors have
1-2 pg/ml IFN-Beta
No elevation of IFN Beta
on other therapies (p=0.4)
IFN Beta in Normal and MS Patient Sera
Detected by ELISA
IFN
Beta
Treated
n=29
N
o
IFN
Treatm
entn-25
N
H
S
n=193
0
10
20
30
40
50
60
70
80
90
100
110
120
IFN-Beta(pg/ml)
16. PBL’s New Human IFNa All Subtype ELISA (Cat. 41115)
is the most sensitive assay kit on the market
Mean LLOD &
LLOQ values on
ALL 12 human
IFN-α subtypes
LLOD & LLOQ values
from IFN-α2 alone;
subtype quantitation
information available
LLOD & LLOQ values from IFN-α2 alone.
No subtype quantitation information available.
*Competitor data obtained from public sources,
provided for comparison only.
0
2
4
6
8
10
12
PBL 41115 PBL 41110 Competitor A Competitor B Competitor C Competitor D(non-
ELISA technology)
LLOD (pg/ml) LLOQ (pg/ml)
18. ELISA assays for soluble Type I and Type II IFN receptors
Soluble Type I and Type II Receptors are elevated in Males
19. sIFNAR2 ELISA Assay
sIFNAR2 Levels in Autoimmunity
Normal n=59
SLE n=111
RA n=16
Sjogren's n=11
Scleroderma n=10
0
2500
5000
7500
10000
10000
35000
p=0.008
sIFNAR2pg/ml
20. Reagent Products
• Proteins
– Human Interferons
– Mouse Interferons
– Rat Interferons
– Other Interferons
– Human Cytokines & Growth
Factors expressed in Human Cells
• Polyclonal Antibodies
– PAbs to Human Interferons
– PAbs to Mouse Interferons
– PAbs to Rat Interferons
– Labeled PAbs
• Monoclonal Antibodies
– MAbs to Human Interferons
– MAbs to Mouse Interferons
– MAbs to Rat Interferons
– MAbs to Porcine Interferons
– Labeled MAbs
21. PBL Assay Science Team
PRaDAS Quality Control
Tom Lavoie Sehrish Ajmal*
Taher Fatakdawala* Karen Zipf*
Robert Niemczyk* Production Team
Xiao-Hong Lin* Daniela Rotaru
Tara Stauffer* Karen Murphy
Michael Skawinski* Rocio Acosta
Lara Izotova* Ksenia Makarenko
Eric Zhu* Natasha Kugakolova
Barbara Schwartz
Nirupama Putcha Collaborators
Sanjna Sanghvi Ray Donnelly (FDA/NIH)
Katherine Carrino-Bednarski Phyllis Conliffe (Smithers Avanza)
Jonathan Ferreira Ira DuBey (Smithers Avanza)