Why is CBS Isothermal -190C Air Storage LN2 Freezer preferred? Temperature Uniformity. CBS Isothermal provides excellent temperature control throughout the freezer, from -190C at the bottom of the freezer to the top, right below the lid. Safety. Storage in liquid nitrogen can make vials shrink. This may cause liquid nitrogen to seep into the vials, which on re-warming, expand and subsequently explode as nitrogen vaporizes inside the vials. Dry storage eliminates this possibility. Also, the Isothermal design provides added user safety by eliminating contact with or splashing of liquid nitrogen. Cross Contamination. Studies have shown that viral, bacterial and fungal pathogens can survive after suspension in liquid nitrogen. Infected samples can cross contaminate other samples in the same liquid nitrogen tank. Dry storage eliminates the possibility of cross contamination.

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CBS Isothermal -196°C dry storage freezers
CBS Standard liquid nitrogen freezers
Cryosystems, vapor-shippers and lab tanks
Controlled rate freezers
Electronic controls, alarms and data recorders
Freezer racks and inventory systems
Custom BioGenic Systems manufactures a wide range of products for industries that include; life sciences, research laboratories, veterinarian, horticulture, pharmaceutical, industrial, scientific and medical, as well as cell and tissue banks.

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CBS Isothermal
Dry Storage Freezers
Why is dry storage important?
Safety.Storage in liquid nitrogen can make vials shrink. This may cause liquid nitrogen to seep into the vials, which on rewarming, expand and explode as nitrogen vaporizes inside the vials. Dry storage eliminates this possibility. Also, the Isothermal design provides added user safety by eliminating contact with or splashing of liquid nitrogen.
Cross Contamination. Studies have shown that viral, bacterial and fungal pathogens can survive after suspension in liquid nitrogen. Infected samples can cross contaminate other samples in the same liquid nitrogen tank. Dry storage eliminates the possibility of cross contamination.

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CBS Isothermal -196C Dry Storage Freezers

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Custom BioGenic Systems -196C Isothermal Dry Storage Freezers Specifications
V1500-AB
V3000-AB
V3000EH-AB
V5000-AB
V5000EH-AB
LN2 Capacity (liters)
30
70
89
93
140
External Width in (mm)
26 (660)
37 (939)
37 (939)
47 (1,219)
47 (1,219)
External Depth in (mm)
37 (939)
48 (1,219)
48 (1,219)
54 (1,371)
54 (1,371)
External Height in (mm)
45 (1,143)
47.5 (1,206)
58 (1,473)
52 (1,320)
58 (1,473)
Usable Interior Height in (mm)
29 (736)
29 (736)
37 (940)
29 (736)
34 (864)
Usable Interior Diameter in (mm)
21 (534)
31 (787)
31 (787)
40 (1,016)
40 (1,016)
Weight Empty lbs(kg)
327 (148)
600 (272)
650 (295)
936 (425)
997 (453)
Weight Full lbs(kg)
383 (174)
720 (327)
808 (367)
1100 (500)
1247 (566)
Maximum 2ml vial capacity
9,100
22,100
25,500
40,300
46,500
Maximum 50ml bag capacity
434
1,120
1,280
1,936
2,208

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Know the CBS Isothermal Freezer
The unique, patented design provides the optimum environment for preserving the long-term viability of samples with uniform temperatures throughout the freezer in the -190°C range, including directly below the lid.

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Know the CBS Isothermal Freezer
No liquid nitrogen inside the sample area eliminates the risk of cross contamination and provides added user safety by eliminating contact with or splashing of liquid nitrogen.

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Know the CBS Isothermal Freezer
The full internal volume is available for storing samples making the most efficient use of freezer capacity. There are no aluminum plates or carousel walls taking up valuable storage space.
The full width lid provides unrestricted access to samples.

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The outstanding temperature uniformity and “dry” storage condition address requirements of the latest European directives for tissue storage, in respect to maintaining cell integrity and preventing contamination.
Know the CBS Isothermal Freezer

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Temperature Uniformity and Liquid Level
In conventional (non CBS) vapor phase freezers, the temperature directly under the lid can be reduced by raising the level of liquid Nitrogen in the bottom.
Whilst improving the temperature uniformity (gradient), this reduces the amount of space available for storing samples in vapor. This also makes the freezer more expensive in terms of the number of samples stored.
In this situation, ask the customer to find out the relationship between temperature gradient and the level of liquid Nitrogen in the freezer, since the specified temperature may depend heavily on the liquid Nitrogen level.
In CBS Isothermal freezers all the internal volume is available for sample storage -free from liquid Nitrogen.

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Temperature Uniformity and Temperature Probe Location
Encourage the customer to check with potential suppliers about the operating temperatures of their freezers and where the temperature sensor is located.
Cold air sinks and therefore the best location for the temperature sensor is at the top, above the samples. On the CBS Isothermal freezers, the sensor is located directly below the lid and above the top of the racks giving the customer confidence that all samples are stored below the indicated {display) temperature -see typical V-1500 mapping data below.
If the sensor is positioned lower down in the freezer, the displayed temperature will look good but can the customer be sure of the temperature of samples higher up?

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Probe #1
Probe #2
Probe #3
Probe #4
Probe #5
Probe #6
An aluminum freezer rack is used for attaching 6 temperature probes to monitor liquid nitrogen vapor temperature.
Spacing between the probes is approximately 4 inches (10 cm).
CBS Isothermal -190C Dry Storage Freezers
Fill to Fill Temperature Study

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-196°C
-194°C
-192°C
-190°C
-188°C
-186°C
-184°C
-182°C
-180°C
-178°C
Probe 1
Probe 2
Probe 3
Probe 4
Probe 5
Probe 6
END OF FILL
LEVEL AT 17"(43CM)
FILLING PROGRAM-LOW SET POINT 10" (25 CM)
HIGH SET POINT 17" (43 CM)
START OF FILL
LEVEL AT 10"(25CM)
Approximately 21 to 24 hours fill to re-fill
10 Minute fill period
6 Temperature probes are placed in a 13 shelf aluminum freezer rack, equally spaced from top to bottom.
CBS Isothermal -190C Dry Storage Freezers
Fill to Fill Temperature Study

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This study shows that as the liquid nitrogen in your CBS Isothermal Freezer evaporates, the internal temperature at probe #1 will rise slightly to -182C. As the re-fill process starts, the temperature at probe #1 will return to the normal operating temperature in the -189C range.
The temperature of the other 5 probes increases very little, ranging between -186C to -196C. Therefore, 85% of the freezer storage space remains below -186C and only the top15% of the space will be slightly warmer in the -182C range.
This study provides temperature data that confirms samples stored in a CBS Isothermal Freezer will be well below critical temperatures.
Your CBS Isothermal Freezer is pre set to re-fill when the liquid nitrogen level reaches a low set point of 10” (25cm) and stop filling when the liquid nitrogen reaches the high set point of 17” (43cm). These filling set points will provide excellent operating parameters. However, they can be changed to suit your specific requirements.
Conclusion

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Temperature Uniformity and Thermal Conductivity
Customer: "Other freezers also offer -190°C vapor storage"
To compete with the Isothermal concept and provide greater uniformity, other manufacturers use the thermal conductivity properties of aluminum and install aluminum plates (frames and carousel walls) inside the freezer. The aluminum conducts heat from the top of the freezer, which promotes evaporation and the resulting Nitrogen vapor rises to cool the top of the freezer.
This approach has two disadvantages;
•Firstly, the more aluminum in contact with liquid Nitrogen, the higher the consumption will be. This is why it is important for the customer to compare the loss rate of the actual models they are considering.
•Secondly, the use of frames and carousels reduces the amount of space inside the freezer for samples. In contrast, the open interior of the CBS Isothermal freezer provides maximum storage capacity without the restrictions of carousels etc.

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Customer: " I prefer Narrow Neck Lids as they keep the cold in"
In an effort to reduce cold air loss and liquid Nitrogen consumption, manufacturers have introduced models with narrow neck openings. Whilst the concept appears attractive, feedback from many customers highlights some practical issues with this design.
Firstly, access to and location of samples is made more difficult. In contrast,
the CBS Isothermal design with Nitrogen vaporvents helps to hold the cold air inside the freezer. This means that full-width lids can be used giving the user unrestricted access for easier sample location.
The following temperature mapping data shows how the temperature inside a V-1500 increases on lid opening. Even after 30 minutes the air temperature at the top of the V-1500 remains well below the critical glass transition temperature of water.
Secondly, if anything is dropped or becomes jammed in the carousel system of a freezer with a narrow neck lid, it becomes almost impossible to retrieve or repair.
Narrow Neck Lids

16. Slide 22of 89
An aluminum freezer rack is used for attaching 6 temperature probes to monitor liquid nitrogen vapor temperature.
Spacing between the probes is approximately 4 inches (10 cm).
CBS Isothermal -190C Dry Storage Freezers
5 Minute Lid Open Temperature Study
Probe #1
Probe #2
Probe #3
Probe #4
Probe #5
Probe #6

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-196°C
-194°C
-192°C
-190°C
-188°C
-186°C
-184°C
-182°C
-180°C
-178°C
0:30:00
12:00:00
12:30:00
13:00:00
13:30:00
14:00:00
14:30:00
15:00:00
15:30:00
16:00:00
16:30:00
17:00:00
17:06:00
17:12:00
Probe 1
Probe 2
Probe 3
Probe 4
Probe 5
Probe 6
5 MINUTE LID OPEN PERIOD
COLD VAPOR FROM THE LOWER LAYERS COOLS THE UPPER LAYERS
TEMPERATURE INCREASES
TEMPERATURE IS STABILIZED TO NORMAL OPERATING
TEMPERATURE
CBS Isothermal -190C Dry Storage Freezers
5 Minute Lid Open Temperature Study
6 Temperature probes are placed in a 13 shelf aluminum freezer rack, equally spaced from top to bottom.
Freezer lid is opened.
Items are stored or retrieved.
Lid is closed.

18. Slide 24of 89
Lower capacity
Round lid opening may restrict access to square racks
Narrow Neck Lids

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Liquid Nitrogen Consumption
Customer: “Other freezers use less liquid nitrogen”
The only way to measure and compare LN2 consumption of freezers is by using the Static Evaporation Rate (also known as Normal Evaporation Rate). This is an industry accepted method for measuring the LN2 consumption of an empty freezer under standardized conditions. (A copy of the method is available on request).
Many manufacturers make claims about lower LN2 consumption and running costs but then don't support this with data in their sales material.
If LN2 consumption is an issue, we recommend that customers ask potential suppliers for the Static Evaporation Rate (SER) for the freezers they are considering so that like-for like comparisons can be made.
It is important that the customer asks for the SER of the specific model they are considering, including built-in options. Some manufacturers only quote a SER for the base model, however the actual SER could be much higher if the customer orders optional aluminum frames or a carousel.

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Series 2301 Auto-Fill and Monitor System
2301 Front Panel Display
Four line LCD readout
Start / Stop Fill
System Status
Output Data to Printer
Fill Level Set Points
Temperature Display –Fahrenheit / Centigrade
Liquid Level –Inch / Centimeter

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Liquid Level Filling Set Points
Your CBS Isothermal Freezer is delivered with factory pre-set liquid level filling set points.
The low set point (start fill) should be set at 10 inches (25 cm).
The high set point (stop fill) should be set at 17 inches (43 cm).
These filling set points will provide excellent operating parameters. However, they can be changed to suit your specific requirements.

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Series 2301 Controller
2301 Features
0-5 volt outputs provide temperature & level data for connection to existing alarm/monitor system
4-20 mA outputs provide temperature & level data for connection to existing alarm/monitor system
Sequential and One-Fill-All-Fill input and output connection for filling systems
New 16 Port Connector
New two level temperature display option
Temp A displays temperature at the lid, approximately 11” (27.94 cm)
Temp B displays temperature inside the storage space, approximately 20” (50.80 cm)
Additional Features
Download data files to PC and be able to convert them to .csv files for use in EXCEL
Ethernet and auxiliary RS485 for future expansion
Dedicated 24VDC signal output to activate a third 24VDC valve to control the LN2 supply
Global Remote Alarm -Dry contact that switches during any alarm condition
Overflow Sensor (Optional)
Fill Timer
Data Log -Report Temp A, Temp. B and level with date and time stamp. Printed or displayed.

23. Slide 29of 89
Series 2301 Features / Specifications
1.Vent Thermocouple
2.Temp. A Thermocouple -Lid temp (located 11” down)
3.Temp. B Thermocouple -Temp (located 20” down)
4.Ethernet Port -For future expansion
5.RS-485 IN
6.RS-485 OUT
7.Sensor Port
8.Lid Switch
9.Fill/Vent In Progress
10.Fill Solenoid Valve Outlet
11.Vent Solenoid Valve Outlet
12.Global Remote Alarm
13.2 Amp Fuse
14.24 V Power Supply
15.Overflow Sensor (Optional)
16.Thermal Printer Port -For printing alarms & data
17.16 Port Connector:
0-5V output for Temp. A, Temp. B, and Level
4-20mA outputs –for Temp. A, Temp. B, and Level
Seq. OFAF Input and Output Connection
18.Auxiliary RS 485 -For future expansion
19.USB Port
1
2
3
4
5
6
7
9
10
11
12
13
19
18
17
8
16
14
15

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The amount of time required to fill a freezer with liquid nitrogen will vary with model. Listed below are the estimated fill times for each CBS Isothermal Freezer.
V1500 –approximately 40 minutes
V3000 –approximately 90 minutes
V5000 –approximately 90 minutes
V5000 EH –approximately 90 minutes
Initial Filling Details
These fill times are based on a conventional 180 liter and 230 liter supply tanks operating at 22 psi.
Fill times may vary with the use of bulk tanks and vacuum jacketed supply lines.