1. What is the function of DNA polymerase I? How replication eye and forks are formed? 2. Explain Okazaki fragments, leading and lagging strands? How Okazaki fragments are involved in DNA replication? 3. Describe the process of DNA replication with proof reading, nick translation, Klenow fragment and involvement of metal ions for DNA polymerase? 4. Explain the characteristics of different types of prokaryotic DNA polymerases? 5. Describe the synthesis of DNA with leading and lagging strands with formation of replisome, sliding clamp and termination steps? 1 FORCE FIELD ANALYSIS 2 FORCE FIELD ANALYSIS Force Field Analysis Any business aspiring to make a change in its operations must use the force field analysis because it is a strategic tool used to take action whenever the cause of a problem in the business is established. The force field analysis is a technique based on the assumption that any situation in the organization that threatens its sustainability has forces that are for or against the organization's stability. The applied remedies will ensure that the business goes back to operate optimally. Since the procurement department has been marred with corruption scandals in the recent past and some employees were fired, the kickback culture can negatively influence the remaining employees, especially the older ones who have served the organization for the past fifteen years (Hayes, 2018). Therefore, the purpose of the force field analysis is to ensure that there is a complete change in the way operations are done in the procurement department and instill a better culture that will enable the procurement department to operate effectively. The CEO may have a good intention to impose the change on the employees for the organization's stability. However, some factors may lead to resistance, and the employees may view it as a witch hunt. Unless there is proper communication, the older stuff will be very reluctant to embrace the organization's new procurement processes. Fear of failure is another threat to the implementation of the change, and a proper strategy must be employed to have the buy-ins of all the stakeholders in the shipping industry. The process of introducing the change will have to follow the Kurt-Lewin three-step models to change. The models entails; unfreeze, change, and then refreeze. In simple terms, the unfreezing process explains the need for the change, and the stakeholders will have a clear explanation of what is needed to make the industry better. Once the people understand the importance of the change and realize that the change is meant for their good, 3 FORCE FIELD ANALYSIS they will embrace it (Hayes, 2018). Finally, after the people have agreed to adopt the change, the new norm will be solidified to be a business culture Cummings, (Bridgman, & Brown, 2016). Identifying the areas of change Identification is the first step in the process of introducing change. The ...