Module iii student's copy 2

PERFECT SCORE BIOLOGY 2009
1
BAHAGIAN SEKOLAH BERASRAMA PENUH DAN
SEKOLAH KLUSTER
KEMENTERIAN PELAJARAN MALAYSIA
Student’s Copy
PERFECT
SCORE
BIOLOGY

2009
MODUL III PAPER 3
QUESTION 1
DISEDIAKAN OLEH:
1. Puan Hasimah binti Azit – Sekolah Seri Puteri, Cyberjaya
2. Puan Natrah binti Mohamad – SMA Persekutuan Kajang
3. Puan Mardiana binti Yusof – SMS Hulu Selangor
4. Puan Sarah Safarina binti Sabri- Kolej Islam Sultan Alam Shah
5. Puan Kamariah binti Zailan – SMA Persekutuan Labu
6. Puan Siti Asma binti Razali – Kolej Islam Klang
7. Puan Noorsyamsinar binti Kasim- SM Sains Selangor

2
Answer all questions.
1. A group of students carried out an experiment to study the effect of temperature to rate of plant
transpiration.
Diagram 1(a) shows an experiment set-up of the apparatus used in this experiment. The apparatus
are prepared and kept in laboratory with difference temperature. The whole experiment in Diagram 1
was repeated using different temperature.
Diagram 1 (b) shows the position of the meniscus at the initial of the experiment. Two points, P and
Q are mark with 10 cm distance at the tube of potometer.
Table 1.1 shows the reading of temperature and location of meniscus in the tube of potometers after 5
minutes.
The location of meniscus in tube (X) of potometer

3
Thermometers after 5 minutes
Table 1.1
Table 1.1
(a) (i) Based on table 1.1 state two observations on the relationship between the
temperature and meniscus.

4
1. ……………………...........................................................................................................................
.........................................................................................................................................................
2. .......................................................................................................................................................
........................................................................................................................................................
[3 marks]
(ii) State the inference which corresponds to the observations in 1 (a)(i)
1. .................................................................................................................................................
......................................................................................................................................................
2. .................................................................................................................................................
........................................................................................................................................................
[3 marks]
(b) Complete the Table 1.1 by recording the temperature and the final position of meniscus.
[3 marks]
(c) (i) Complete the Table 1.2 base on the experiment that was carried out.
Variables Method to handle the variable
Manipulated variable
.......................................................
.......................................................
.......................................................
.......................................................
Responding variable
.......................................................
.......................................................
.......................................................
.......................................................
Controls variable
.......................................................
.......................................................
.......................................................
.......................................................
[3 marks]
Table 1.2
(ii) The following list is part of the apparatus and material used in this experiment.
Thermometer, stop watch, ruler, capillary tube, plant leaves, water

5
Complete Table 1.3 by matching each variable with the apparatus and material used in this
experiment.
Variable Apparatus material
Manipulated
Responding
Controlled
[3 marks]
Table 1.3
(d) State the hypothesis for this experiment.
.....................................................................................................................................................................
.....................................................................................................................................................................
.....................................................................................................................................................................
[3 marks]
(e) (i) Base on the Table 1.1, construct the table and record the results of this experiment which includes
following aspect:
• Temperature (o
C)
• The distance of meniscus movement (cm)
• Rate of transpiration (cm/minute) [3 marks]
(ii) On the graph paper, draw the graph of rate of transpiration against the
temperature.
[3 marks]

6
(iii) Explain the relationship between the rate of transpiration and the temperature
based on the graph.
.......................................................................................................................................................................

7
.......................................................................................................................................................................
.......................................................................................................................................................................
[3 marks]
(f) Based on the experiment, what can you deduce about the transpiration?
........................................................................................................................................................................
........................................................................................................................................................................
........................................................................................................................................................................
[3 marks]
(g) In another experiment, the apparatus is located under the sun shine.
Predict the observation and explain the results of the experiment.
.....................................................................................................................................................................
.....................................................................................................................................................................
......................................................................................................................................................................
[3 marks]
Question 2:
An experiment was done to study the effect of pH on the activity of amylase. Three test tubes labelled P,
Q and R are filled with 10ml of amylase and later filled with 40ml distilled water, 40ml hydrochloric acid
and 40ml sodium bicarbonate respectively.

8
The 50ml of starch suspension was put into each test tube and the contents were stirred.
Every two minutes a drop of the mixture from each test tube was dropped into a drop of iodine solution on
a white tile.
Changes in colour of iodine are observed. The figure shows the set of apparatus of the experiment.
(a)
(i)
State the observations that can be made from the experiment.
1. ……………………………………………………………………………….
………………………………………………………………………………..

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2. ……………………………………………………………………………….
……………………………………………………………………………….
[3 marks]
(ii) State the inferences for the observations made.
1. ……………………………………………………………………………….
………………………………………………………………………………..
2. ……………………………………………………………………………….
……………………………………………………………………………….
[3 marks]
b) Record the time taken when the iodine solution stopped changing colour in the boxes provided.
[3 marks]
c) Complete the table below.
Variable Particulars to be implemented
………………………………..
How to alter the manipulated variable?
……………………………………………………..
……………………………………………………..
Responding variable
………………………………..
How to determine the responding variable?
. ……………………………………………………..
……………………………………………………..
Constant variable
.
………………………………..
How to maintain the constant variable?
……………………………………………………..
……………………………………………………..
(3 marks)
e) State the hypothesis for this experiment.
……………………………………………………………………………………………………….
………………………………………..……………………………………………………………..
[3 marks]

10
f) Based on the results of the experiment, record the results in a table
[3 marks
g) What is the optimum pH for the activity of amylase? Give reasons for your answer.
…………………………………………..…………………………………………………………….
………………………………………….……………………………………………………………..
[3 marks]
h) Explain the relationship between the time taken for change in colour of iodine from yellowish brown
to blue black and different pH medium.
……………………………………………………………………………………………………….
……………………..………………….……………………………………………………………..
……………………..………………………………………………………………………………..
[3 marks]
i) Predict the time taken for change in colour of iodine from yellowish brown to blue black if 40ml of
amylase is used in boiling tube P. Explain your answer.
…………………………………………………………………………………………………….
…………………………………………..………………………………………………………..
…………………………………………………………………………………………………….
[3 marks]
j) Based on the result of the experiment, define what an enzyme is.
……………………………………………………………………………….
………………………………………………………………………………..

11
………………………………………………………………………………..
[3 marks]
k) From your biological knowledge, match the substrate with the enzymes below:
Protein, amylase, trypsin, maltose, starch, maltase, pepsin.
. ……………………………………………………………………………….
………………………………………………………………………………..
……………………………………………………………………………….
………………………………………………………………………………..
[3 marks]
Question 3:
A group of students carried out an experiment to study the inheritance of the colour of flowers in a
plant. The colour of flower is controlled by single pair of alleles, red and white.
The students used red and white button to represents the alleles for red and white flowers.
Two black plastic bag, A and B, each containing a mixture of 50 red buttons and 50 white buttons
were used to illustrate the cross.

12
The students carried out the experiment as follows:
Step 1: One button was drawn at random from each bag.
Step 2: The colour of the button was noted and recorded in a table.
Step 3: The buttons were returned to their respective bags.
Step 4: The bags A and B were shaken to mix up the button randomly.
Step 5: Step 1 to 4 were repeated until a total of 20 times.

13
(a) (i) State two observations from the experiment based on the results shown in Table 1.1
[3 marks]
1. …………………………………………………………………………………………………..
…………………………………………………………………………………………………..
2. …………………………………………………………………………………………………..
………………………………………………………………………………………………….
(ii) State one inference from each observation in 1(a)(i)
[3 marks]
1. …………………………………………………………………………………………………..
…………………………………………………………………………………………………..
2. …………………………………………………………………………………………………..
………………………………………………………………………………………………….
(b) Complete table 1.2 based on the experiment that was carried out.
Variable Method to handle the variable
Manipulated variable:
…………………………..
…………………………………………………………………………
………………………………………………………………………….
Responding variable:
…………………………..
…………………………………………………………………………
………………………………………………………………………….
Controlled variable:
…………………………..
…………………………………………………………………………
………………………………………………………………………….
[3 marks]
(c) State the hypothesis for the experiment. [3 marks]
……………………………….………………………………………………………………………………..
…………………………………………………………………………………………………………………
(d) (i)If the allele for red flowers ( R) is dominant to the allele for white flower (r) , write down the
genotypes of all the draws in the table 1.1 using the letter R and r. [3 marks]

14
(ii) Determine and write down the phenotype of each draw in table 1.1 From the results in table 1.1,
calculate the ratio of the phenotypes obtained from the experiment. [3 marks]
…………………………………………………………….…………………………………………..
………………………………………………………………………………………………………….
(e) Explain the results obtained in 1(d)(ii) by using a genetic diagram. [3 marks]
(f) State the effect of the number of draws on the result obtained. [3 marks]
…………………………………………………………….…………………………………………..
………………………………………………………………………………………………………….
(g) Based on the experiment on the inheritance of the colours of flowers that the students had done,
define Mendel’s First Law of inheritance.
[3 marks]
…………………………………………………………….…………………………………………..
………………………………………………………………………………………………………….
(h) The experiment is repeated by increasing the numbers of red and white buttons from 50 to 100 in
each bag. Predict the ratio of the phenotypes that you would obtain.
[3 marks]
…………………………………………………………….…………………………………………..
.……………………………………………………………………………………………………….
(i) Classify the list of materials shown below according to their function in the experiment.
[3 marks]
Material Function

15
Question 5
An experiment is carried out to investigate the content of vitamin C in certain fruit juices. The method of
experiment is conducted as follows:
1. Preparation of fruit juices shown in the diagram 5.0 below.
DIAGRAM 3.0
2. The steps is repeated for pineapple and orange juice
3. Standard ascorbic acid is used to decolorize the DCPIP as shown in the diagram 5.1
4. The experiment is repeated by using lime and pineapple juices. The result shown in the Diagram
5.2
The squashed is
filtered to obtain the
juice.
Fruit is squashed
Fruit
juices
Diagram 5.0
5 ml syringe
Ascorbic
acid 0.1%
1 ml
DCPIP
0.1% (dark blue) Colourless
Volume of ascorbic
acid 0.1% used:

16
Diagram 5.2 shows the result of an experiment.
Fruit juice Before Experiment After Experiment Volume of fruit juice
is used to
decolorize DCPIP
solution / ml
Lime juice

17
Pineapple juice
(a) (i) Based on Table 1.0, state two observations from the experiment.
[3 marks]
1. ..................................................................................................................................
………………………………………………………………………………………………
2. ………………………………………………………………………………………………
………………………………………………………………………………………………
(ii) State the inferences for each of the observation in 1(a)(i) [3 marks]
Observation 1:
..................................................................................................................................
………………………………………………………………………………………………
Observation 2:
..................................................................................................................................
………………………………………………………………………………………………
(b) Complete Table 3.0 by recording the volume of fruit juices needed to
decolourise DCPIP solution. [3 marks]
Type of fruit juices Volume of fruit juice required to
decolourise DCPIP solution (ml).
Lime juice
Pineapple juice
Orange juice

18
(c)(i) Complete Table 2 based on this experiment.
………………………….
………………………………………………
………………………………………………
.
Responding variable
………………………….
………………………………………………
………………………………………………
Controlled variable
………………………….
………………………………………………
………………………………………………
[3 marks]
[3 marks]
..................................................................................................................................
………………………………………………………………………………………………
………………………………………………………………………………………………
(e) Concentration of vitamin C in the fruit juices is calculated using formula
Concentration of vitamin C = Volume of ascorbic acid used to decolourise
DCPIP solution / volume of fruit juice used to
decolourise DCPIP solution x 1.0 mg / dm3.
Construct a table and record the results of the experiment which includes the following
aspects:
Type of fruit juices
Volume of fruit juices required to decolourise DCPIP solution.
Concentration of vitamin C [3 marks]

19
(f) Explain the relationship between the volume of fruit juice required to decolourise DCPIP
solution and the concentration of vitamin.
[3 marks]
..................................................................................................................................
……………………………………………………………………………………………
……………………………………………………………………………………………..
(g) The following lists are some of the apparatus and material used in this experiment.
Syringe 5.0 ml, watermelon juice, specimen tube, DCPIP solution, 0.1
% ascorbic acid solution, scalpel.
Categorise apparatus and materials used in this experiment.
[3 marks]
(h) (i) If lime juice is exposed to the air for several hours, predict the volume of lime juice
required to decolourise DCPIP solution. [3 marks]
............................................................................................................................................

20
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
.
(ii) explain the relationship between volume of lime juice required to decolourise DCPIP
solution and the time based on (h)(i).
[3 marks]
............................................................................................................................................
……………………………..………………………………………………………………………
……………………………………………………………………………………………………..
(i) Based on this experiment, define vitamin C operationally.
[3 marks]
..................................................................................................................................
………………………………………………………………………………………………
Question 6 :
A group of students carried out an experiment to determine the concentration of external solution which
is isotonic to the cell sap of a plant.
Figure1.1 shows the set up of apparatus for this experiment. By using a cork borer, a cylindrical potato
strip was obtained. The potato strip was cut into 5 cm long.
After 30 minutes , the potato strip is measured again and the final length of potato strip is recorded. The
measurement of potato strip is shown in figure 1.2.
The experiment in figure1.1 was repeated by using different concentration of sucrose solutions.
Potato strip
(initial length –
5cm)
0.0% Sucrose
solution
Figure 1.1
Ruler
Potato strip

21
Figure 1.2
Final length of potato strip is:
5.8 cm

22
Table 1.1 shows the results of the experiment.
Concentr
ation
of
sucrose
solution
(%)
Potato strips after being immersed in solution Final length of
potato strip
(cm)
0.5
5%
10%
15%
TABLE 1.1
(a) Measure and record the length of potato strips and complete the table 1.1.

23
(b) (i) Based on Table 1.1 state two observations that can be made from the experiment concerning the
length of cylindrical potato strips.
............................................................................................................................................
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
.
[ 3 marks]
(ii) State the inference which corresponds to the observation in 1(b)(i).
............................................................................................................................................
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
[ 3 marks]
c) Complete Table 1.2 based on this experiment. State the type of variable of the experiment and
method to handle the variable.
Responding variable
Controlled variable
[ 3 marks]
Table 1.2

24
............................................................................................................................................
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
[ 3 marks]
(e) (i) Based on table 1.1 construct a table and record the results of the experiment
which includes the following aspects:
o concentration of sucrose solutions
o initial length
o final length
o percentage change in length of potato strips
[3 marks]
Aspect Concentration of sucrose solutions (%)
0 0.5 5 10 15
Initial length/cm
Final length/cm
Change in length/cm
Percentage change in length/%

25
(ii) On the graph paper provided, draw the graph of the percentage change in length of
the potato strips against the concentration of the sucrose solutions .
[ 3 marks]
(iii) Based on the graph in (d )(ii), state the concentration of the sucrose solution which is

26
isotonic to the concentration of the cell sap of the potato strip. Explain your answer.
............................................................................................................................................
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
[ 3 marks]
(f) Based on this experiment, what can you deduce about osmosis ?
............................................................................................................................................
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
[ 3 marks]
(g) The potato strip that was immersed in 15% sucrose solution is wipe dry and immerse into the
0.2% sucrose solution.
Predict the changes that occur to the strips?
............................................................................................................................................
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
[ 3 marks]
(h) Classify the various sucrose concentrations used in this experiment by comparing
with the osmotic concentration of the cell sap.
Sucrose concentrations (%) Types of sucrose solution compared to the
osmotic concentration of cell sap
[ 3 marks]
Question 7

27
An experiment is carried out to investigate the effect of environmental factor on the activity of
yeast.
Yeast reacts with sucrose solution to produce alcohol and carbon dioxide gas.
The apparatus as shown in Diagram 1.1 is set up.
1cm3
of 1% yeast suspension is transferred using a pipette into a boiling tube P containing 5
cm3
of 1% sucrose solution. The initial level of the coloured liquid in the U tube is set at 0.0 mm.
After exactly 10 minutes, the level of the coloured liquid is observed as shown in Diagram 1.
Diagram 1.1
The experiment is repeated by using boiling tubes Q, R and S fill with same volume of sucrose
solution. The concentrations of solution used are 2%, 3% and 4% .

28
The changes of the coloured liquid levels in the U-tube are shown in Diagram 1.2
Level of coloured liquid
Boiling tube P (1%) Boiling tube Q (2%) Boiling tube R (3%) Boiling tube S (4%)
25.0 mm 35.0mm 45.0mm 55.0mm
Diagram 1.2 (3 marks)
(a) (i) Based on the results in Diagram 1.1 and 1.2 , state two observations that can be
made from the experiment.
............................................................................................................................................
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
[ 3 marks]
(a) (ii) Write down an inference for each of the observations in (a).
............................................................................................................................................
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
[ 3 marks]
(c) Record the levels of the coloured liquid in the U-tubes in the spaces provided in Diagram
1.2.
[3 marks]

29
(d) (i) Complete Table 1.1 based on this experiment
Responding variable
Controlled variable
Table 1.1 [ 3 marks]
(ii). The following list of apparatus and materials are used in this experiment.
Pipette, 1% yeast solution, scale, coloured liquid, 2% to 5% sucrose solution.
Complete the table 1.2 below by matching each variables with the materials used in this
experiment
Variables Apparatus Materials
Manipulated
Responding
Constant
[ 3 marks]
Table 1.2
(e) State the hypothesis for the experiment ?
............................................................................................................................................
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
[ 3 marks]
(f) (i) The volume of the carbon dioxide produced by the yeast in the boiling tube can be
calculated using the following formula:
The volume of the carbon dioxide produced (ml) = The cross-section of the glass tube
(mm2
) X the distance travelled by the
coloured liquid (mm)

30
The volume of carbon dioxide produced (mm3
)
The rate of carbon dioxide produced = ---------------------------------------------------------------------
Time taken for the coloured liquid to travel (s)
Based on Figure 2, record the results of this experiment obtained in (c) in a table, given that
the cross section of the glass tube is 3 mm2
. Your table should include the following aspects:
• The concentration of the sucrose solution
• The distance travelled by the coloured liquid
• The rate of carbon dioxide produced
(3 marks)

31
(ii) From the table in (f) (i) above, draw a graph of the concentration of the sucrose solution
against the rate of carbon dioxide produced.
[ 3 marks]

32
(g) From the table you have drawn in (f) above, explain the relationship between the
concentration of sucrose solution, the height of the coloured liquid and the rate of carbon
dioxide produced by the yeast.
............................................................................................................................................
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
[ 3 marks]
(h) From the experiment, what can be deduced about fermentation of yeast ?
............................................................................................................................................
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
[ 3 marks]
(i) If the sucrose solution concentration is increased to 6%, predict the rate of carbon
dioxide produced by the yeast in 10 minutes.
............................................................................................................................................
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
[ 3 marks]
Question 8

33
An experiment is carried out to investigate the pollution level of a river in W district.
Figure 1.1
Three samples of water are collected from the three location respectively, and named water
sample P, water sample Q and water sample R. The samples are brought to the school lab and
tests are conducted.
First, 100ml of the water sample P is measured using a measuring cylinder and poured into a
clean reagent bottle. 1ml of 0.1% methylene blue solution is added into the water sample using
a syringe. The mixture is stirred carefully with the needle of the syringe. The reagent bottle is
closed quickly and kept in a dark cupboard (Figure 1.2). The stop watch is started (Table 1.1)
and the colour of the solution is observed from time to time to determine the time taken for the
0.1% methylene blue solution to decolourise.
Figure 1.2
The above steps are repeated with water sample Q and water sample R. The time taken for the
solution to decolourise is recorded in table 1.1.
R
Q
P
orchard
Housing
area
Food
factory

34
Water
sample
location Treatment
Time shown on the stop
watch when the
methylene blue solution
decolourises
Time taken
(min)
P
Q
R
Table 1.1
(a) (i) Based on the results in Table 1.1, state two observations for the experiment.
............................................................................................................................................
………………………………..……………………………………………………………………

35
……………………………………………………………………………………………………
(3 marks)
(ii) Write down an inference for each of the observations in (a).
............................................................................................................................................
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
(3 marks)
(b) Record the time taken for the decolourisation of 0.1% methylene blue solution in the
spaces provided in Table 1.1.
(3 marks)
(c) Complete Table 2.1 based on this experiment
Responding variable
Controlled variable
Table 2.1 (3 marks)

36
(d) The following list of apparatus and materials are used in this experiment.
0.1% methylene blue solution, water samples, stop watch, syringe, reagent bottle,
measuring cylinder,
Complete the table 1.4 below by matching each variables with the materials used in this
experiment
Variables Apparatus Materials
Manipulated
Responding
Constant
(3 marks)
e. State the hypothesis for the experiment ?
............................................................................................................................................
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
(3 marks)
f. Based on Table 1.1, record the results of this experiment obtained in (c) in a table. Your
table should include the following aspects:
- The water sample
- The time taken for the 0.1% methylene blue to decolourise
- The BOD level of the water samples.
(3 marks)
(g) From the table you have drawn in (f) above, explain the relationship between the
location from orchard area and time taken for the decolourisation of 0.1% methylene
blue solution and the B.O.D. level of the water sample.
............................................................................................................................................
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
(3 marks)

37
g. From the experiment, what can be deduced about B.O.D. level of water samples ?
............................................................................................................................................
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
(3 marks)
h. If the number of houses in housing area increase predict the time taken and the level of
B.O.D. for water in location Q.
............................................................................................................................................
………………………………..……………………………………………………………………
……………………………………………………………………………………………………
(3 marks)
END

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