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Introduction to
Histology:
The science of Pathology
Objectives
 Introduction
 Applicable for
 Importance
 Requirement
 Various stain
Where it is applied??
 Microscopic anatomy (histology)
 Gross anatomy
 Other basic science courses
• Biochemistry
• Physiology
• Microbiology
• Pathology
• Pharmacology
WHY ??????
 Develop mental image
 Understand normal function of cell
 Differentiate slides
 Identify organs
 Exam
What things Need for it???
 Microscope
 Knowledge of Stains used
 Characteristics of slide
 Imagination
 H&E pencil
 A good book
Many stains……
 H&E (hematoxyllin and eosin)
 PAS (periodic acid schiff)
 Acid fast, toludine blue
 Masson trichome
 Van Gieson
 Orecine
 Silver nitrate
 Elastin
 Osmic acid
 Wright and Giemsa stain
 Many other…
Difficult to observe:
 diameter: 5 to 50um or in nm
 Like:
 Cell membrane
 Cytoplasmic organelles
 Nucleolus
 Chromatin
Epithelium:
The covering and lining
Objectives
I. Definition of Epithelia.
II.Epitheloid tissue
III.Function of Epithelia
IV.Classification of Epithelial cells.
V.Basement membrane
VI.Epithelial intercellular junctions
VII.The apical surface of epithelial
cells.
EPITHELIA
group cells
cover the outer surface of the body or the
luminal surfaces of body cavities
single layered to multiple layered .
Epitheloid tissue
 Aggregation of cell
 No free surface
 Example
 Parenchyma of adrenal gland
 Leydig cell of testis
 Luteal cell of ovary
Function
Classification of Epithelial tissues
Stratified columnar
Simple Squamous Epithelium
• flattened cells
• Oval and large nucleus
• Bulging of cell surface due to large nucleus
• Eg. Lines the:
 alveoli of lungs,
 the free surfaces of peritoneum,
 pleura and
 pericardium
 blood vessels (endothelium)
 heart (endocardium) (mesothelium)
Simple cuboidal Epithelium
• like squares or cuboid
• Nuclei is rounded and centrally placed.
• lines the:
 follicles of thyroid gland,
 kidney tubules,
 germinal layer of ovary and
 ducts of various glands .
Simple Columnar Epithelium
• Height >width .
• nuclei are oval & elongated toward the basement
membrane.
• lines the
 mucous membrane of the stomach
 large intestine
 Some part of bronchioles
 Uterine tube
Columnar epithelium
 Striated border
 Cliated
 Non-ciliated
Cell present:
 Goblet cell: intestine
Columnar Epithelium Striated Border
• vertical striations on free surface.
• Brush border
• Electronic view: microvilli
• Non motile
• typically seen in small intestine.
Ciliated columnar
 Large bronchioles
 Uterine tube
Pseudo stratified Epithelium
• appears to be multi-layered but actually there is
only one layer of cells.
• Cell of different height
• position of nucleus at different levels
• bears hair like projections called cilia.
• Mostly seen in trachea and
large bronchi .
Pseudo Stratified Columnar Epithelium
(With Stereocilia)
• Stereocilia as seen in epididymis .
• Stereocilia are actually long microvilli and not cilia.
Stratified Squamous Epithelium
(Non–Keratinised )
• only the most superficial cells are flattened .
• deepest are columnar
• middle layers they are polyhedral,
• while the more superficial layers show increasing
degrees of flattening .
• no superficial keratinized zone ; flattened cells
are seen in topmost layer .
• lining some internal organs like
 Oesophagus,
 Cornea, Tongue and the vagina .
Stratified Squamous Epithelium
(Keratinized)
 The surface layer is made up of keratin which
appears as fibers .
 Keratinization
 typically seen in epidermis of skin.
Stratified cuboidal
 Excretory duct of salivary gland, pancrease
Stratified columnar
 Male urethra
Transitional Epithelium
• Have several layers of cells with round nuclei.
• The deepest cells are columnar or cuboidal.
• The middle layers are made up of polyhedral or pear-
shaped cells.
• Superficial layer shaped as
umbrella.
• These are mostly found in
urinary tract.
Specialized structure:
On the apical surface of epithelial cells
 Striated border
 Steroclia
 cilia
Sterocilia
Basement membrane
(15-20nm)
(3nm)
20-25nm
introduction to Histology and epithelia

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introduction to Histology and epithelia

  • 2. The science of Pathology
  • 3. Objectives  Introduction  Applicable for  Importance  Requirement  Various stain
  • 4. Where it is applied??  Microscopic anatomy (histology)  Gross anatomy  Other basic science courses • Biochemistry • Physiology • Microbiology • Pathology • Pharmacology
  • 5. WHY ??????  Develop mental image  Understand normal function of cell  Differentiate slides  Identify organs  Exam
  • 6. What things Need for it???  Microscope  Knowledge of Stains used  Characteristics of slide  Imagination  H&E pencil  A good book
  • 7. Many stains……  H&E (hematoxyllin and eosin)  PAS (periodic acid schiff)  Acid fast, toludine blue  Masson trichome  Van Gieson  Orecine  Silver nitrate  Elastin  Osmic acid  Wright and Giemsa stain  Many other…
  • 8. Difficult to observe:  diameter: 5 to 50um or in nm  Like:  Cell membrane  Cytoplasmic organelles  Nucleolus  Chromatin
  • 10. Objectives I. Definition of Epithelia. II.Epitheloid tissue III.Function of Epithelia IV.Classification of Epithelial cells. V.Basement membrane VI.Epithelial intercellular junctions VII.The apical surface of epithelial cells.
  • 11. EPITHELIA group cells cover the outer surface of the body or the luminal surfaces of body cavities single layered to multiple layered .
  • 12. Epitheloid tissue  Aggregation of cell  No free surface  Example  Parenchyma of adrenal gland  Leydig cell of testis  Luteal cell of ovary
  • 13.
  • 15. Classification of Epithelial tissues Stratified columnar
  • 16. Simple Squamous Epithelium • flattened cells • Oval and large nucleus • Bulging of cell surface due to large nucleus • Eg. Lines the:  alveoli of lungs,  the free surfaces of peritoneum,  pleura and  pericardium  blood vessels (endothelium)  heart (endocardium) (mesothelium)
  • 17. Simple cuboidal Epithelium • like squares or cuboid • Nuclei is rounded and centrally placed. • lines the:  follicles of thyroid gland,  kidney tubules,  germinal layer of ovary and  ducts of various glands .
  • 18. Simple Columnar Epithelium • Height >width . • nuclei are oval & elongated toward the basement membrane. • lines the  mucous membrane of the stomach  large intestine  Some part of bronchioles  Uterine tube
  • 19. Columnar epithelium  Striated border  Cliated  Non-ciliated Cell present:  Goblet cell: intestine
  • 20. Columnar Epithelium Striated Border • vertical striations on free surface. • Brush border • Electronic view: microvilli • Non motile • typically seen in small intestine.
  • 21. Ciliated columnar  Large bronchioles  Uterine tube
  • 22. Pseudo stratified Epithelium • appears to be multi-layered but actually there is only one layer of cells. • Cell of different height • position of nucleus at different levels • bears hair like projections called cilia. • Mostly seen in trachea and large bronchi .
  • 23. Pseudo Stratified Columnar Epithelium (With Stereocilia) • Stereocilia as seen in epididymis . • Stereocilia are actually long microvilli and not cilia.
  • 24. Stratified Squamous Epithelium (Non–Keratinised ) • only the most superficial cells are flattened . • deepest are columnar • middle layers they are polyhedral, • while the more superficial layers show increasing degrees of flattening . • no superficial keratinized zone ; flattened cells are seen in topmost layer . • lining some internal organs like  Oesophagus,  Cornea, Tongue and the vagina .
  • 25. Stratified Squamous Epithelium (Keratinized)  The surface layer is made up of keratin which appears as fibers .  Keratinization  typically seen in epidermis of skin.
  • 26. Stratified cuboidal  Excretory duct of salivary gland, pancrease Stratified columnar  Male urethra
  • 27.
  • 28. Transitional Epithelium • Have several layers of cells with round nuclei. • The deepest cells are columnar or cuboidal. • The middle layers are made up of polyhedral or pear- shaped cells. • Superficial layer shaped as umbrella. • These are mostly found in urinary tract.
  • 29. Specialized structure: On the apical surface of epithelial cells  Striated border  Steroclia  cilia
  • 31.