80 µl of 10^-5 dilution of E. coli culture was plated on LB agar and incubated at 37 degree Celsius overnight. Next day, you observed 212 colonies on this plate. Based on this information, calculate the viable count (cfu/mL) of the original E. coli culture. Solution Colony forming units (CFU)/mL (from plate C) = (no. of colonies x dilution factor) / volume of culture plate Number of colonies observed is = 212 Dilution factor is = 10^5 Volume of culture plate =80 µl Colony forming units (CFU)/mL (from plate C) = 212*10^5/ 0.08 ml = 26.50* 10^7 CFU/ml.