Phdhepatoprotective
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Phytochemical analysis found that petroleum ether, alcoholic, and aqueous basic extracts of the plant contained phenols, tannins, flavonoids and other compounds. DPPH, H2O2, and OH- ion antioxidant assays showed the ethanol extract had the best antioxidant activity with IC-50 values of 38.37, 85.27, and 202.79 μg/ml respectively. Therefore, the hepatoprotective activity of the ethanol extract will be assessed.
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This document summarizes a study that analyzed the phenolic profile and antioxidant activity of extracts from different parts (roots, leaves, fruits) of the Citrullus colocynthis plant. Reverse phase high performance liquid chromatography was used to simultaneously quantify phenolic acids and flavonoids in ethanol and hexane extracts. The major phenolic compounds identified were ferulic acid, vanillic acid, p-coumeric acid, gallic acid, p-hydroxy benzoic acid, chlorogenic acid, quercetin, myricetin and catechin. Total phenolic and flavonoid contents were highest in ethanol extracts of the leaves. Ethanol extracts of the leaves also exhibited the highest antioxidant and DPPH radical sc
40, antioxidant, 1
This document reports on a study that evaluated the phytochemical content and antioxidant activities of different extracts of Jatropha curcas leaves. Qualitative phytochemical screening showed the presence of flavonoids, tannins, phenolics, saponins, coumarins, sterols and triterpenes in the leaves. The total phenolic and flavonoid contents were highest in the successive methanolic extract and crude methanolic extract, respectively. The successive methanolic extract exhibited the strongest antioxidant activities in DPPH radical scavenging, total antioxidant, and reducing power assays. HPLC analysis of the successive methanolic extract identified catechin as a major polyphenolic compound. The results confirm the presence of
Gareth Fenn Journal of analytical 2016
The document summarizes an experiment to determine the polyphenol and caffeine contents that can be extracted from spent coffee grounds, as well as the antioxidant capacities of the extracted compounds. Two types of spent coffee grounds (Costa Robusta and Nandos Arabica) were extracted using ultrasonic-assisted extraction. The extracts were then analyzed using HPLC, FRAP, DPPH, and Folin-Ciocalteu assays to determine caffeine, chlorogenic acid, total polyphenol, and antioxidant levels. The Robusta extract showed higher scavenging ability and electron transfer capacity compared to the Arabican extract based on FRAP and DPPH assays.
Optimization of Polyphenol Content in Green Tea and studying its Antioxidant ...
The aim of my project is to optimize the level of Polyphenol content present in Green Tea at various
temperatures. During the project various combinations of temperatures and time intervals were used to obtain
the Green Tea extract and the polyphenol content of all these extracts were estimated. Both unfermented and
artificial fermentation condtions were used to obtain the extract. The extracts were further used to screen the
various contents of the extract both normally and after purifying the sample with PVP (Polyvinyl pyrrlidone).
The extract with the highest content of polyphenols were further used to estimate the antioxidant property after
purification with column chromatography
Optimization of Polyphenol Content in Green Tea and studying its Antioxidant ...
The aim of my project is to optimize the level of Polyphenol content present in Green Tea at various
temperatures. During the project various combinations of temperatures and time intervals were used to obtain
the Green Tea extract and the polyphenol content of all these extracts were estimated. Both unfermented and
artificial fermentation condtions were used to obtain the extract. The extracts were further used to screen the
various contents of the extract both normally and after purifying the sample with PVP (Polyvinyl pyrrlidone).
The extract with the highest content of polyphenols were further used to estimate the antioxidant property after
purification with column chromatography.
ChindavongPoster_pbm
1. Researchers investigated fractionating pine cone extracts (PCT and PPC) using solid phase extraction columns to identify active compounds, focusing on C18 column separation.
2. C18 column fractionation indicated PCT contained more non-polar compounds eluted by ethyl acetate, while PPC had more polar compounds eluted by acidic methanol.
3. All PCT fractions showed higher antioxidant activity per mg than PPC fractions, though PPC fractions had higher protein binding activity suggesting better immune activity.
A Cycloalkanol from Derivatization Studies on Vanillin: Evaluation of Antioxi...
A Cycloalkanol from Derivatization Studies on Vanillin: Evaluation of Antioxi...BRNSSPublicationHubI
This document describes a study that derivatized vanillin through acetylation, O-demethylation, reduction, and oxidation reactions. The identities of the derivatives were determined using IR spectroscopy. The antioxidant activities of vanillin and the derivatives were evaluated using the DPPH radical scavenging assay and compared to vitamin C as a standard. The reduced derivative was unexpectedly found to be a cycloalkanol rather than a phenolic compound. The reduction and acetylation derivatives showed the highest antioxidant activities, indicating these modifications enhanced vanillin's antioxidant properties.Determination of phenolic compounds
Research Paper PPT on determination of phenolic compounds in apples and processed apple products by HPLC.
Comparison of antioxidant activity of ethanolic extracts of propolis obtained...
The Particular antioxidant actions in the ethanolic extracts regarding propolis obtained simply by d...
Phytochemical and Biological Evaluation of Cassia tora, L. Seeds
The document summarizes a study that evaluated the hepatoprotective activity and fatty acid composition of Cassia tora L. seeds. Researchers extracted the seeds with hexane, ethanol, and a combination to obtain total, defatted, and lipid extracts. GC-MS analysis of the lipid extract identified 27 fatty acids. Rats were treated with extracts or Silymarin after carbon tetrachloride induction of hepatic injury. Biomarkers and histology showed the extracts comparable to Silymarin in protecting liver function, with the total extract more effective. The study demonstrated hepatoprotective effects of C. tora seed extracts.
Synthesis of E, E-dibenzalacetone (E, E-DBA) Lab ReportN.docx
Synthesis of E, E-dibenzalacetone (E, E-DBA) Lab Report
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Abstract
The main purpose behind this experiment was to synthesize E, E-dibenzalacentone by use of a based-catalyzed aldol condensation reaction. Secondly, this experiment was aimed at identifying characteristics of crude and purified samples of E, E-dibenzalacentone by use of the melting point determination method. The results from this experiments showed that the synthesis process yielded 65% crude E, E-dibenzalacentone and 56% yield for purified E, E-dibenzalacentone. When it comes to the techniques that were used, the researchers employed Hirsch funnel and solid-liquid extraction as part of the separation techniques. Lastly, recrystallization of the E, E-dibenzalacentone was also used as a purification technique.
Introduction
The synthetic goal of this experiment was to make use of a based-catalyzed aldol condensation in the entire process of synthesizing o E, E-dibenzalacentone. The other main goal of this experiment was to identify or to characterize crude and purified samples of E, E-dibenzalacentone by use of the melting point determination
Results
The results of the synthesis of E, E-dibenzalacentone are summarized below
Compound
Molar mass(g/mol)
Volume/mass moles
Yield
Mp( °c)
Benzaldehyde (density1.043g/mL)
106.2 g/mol
0.250 mL
0.26 g
0.002 mol
N/A
N/A
Acetone(density 0.788g/mL)
58.08 g/mol
0.1 mL
0.07g
0.001 mol
N/A
N/A
E,E-DBA(a yellow solid)
234.24 g/mol
N/A
Theoretical
0.234g
0.001 moles
Literature Value
110-111 °c
E,E-DBA(a yellow solid)
234.24 g/mol
N/A
Actual
Crude: 0.65 g=65%
Purified:0.15 g=56%
Actual
101-103Co
Crude
108-109Co
Discussion
The E, E-dibenzalacentone was prepared by combining 0.250 mL of Benzaldehyde, 0.1mL of reaction grade acetone, 0.5 mL of 95% ethanol and 1 mL of a catalyst solution in a 10 mL round bottom flask. The catalyst solution that was used in this experiment has been prepared earlier by dissolving 10g of sodium hydroxide in 100 mL of water and 75 mL of 95% ethanol. The main purpose of this catalyst was to speed up the reaction process. A micro scale condenser was attached to the round bottom flask and the reactants were gently mixed for about 15 minutes, until a solid started forming. Then the crude solid was isolated by pouring the mixture into a Hirsch funnel attached to the vacuum filter, thus allowing the solid crude E, E-dibenzalacentone to be trapped by the filter paper in the Hirsch funnel.
An RB flask was de-attached from the condenser after which it was rinsed with 20 mL of de-ionized water at one time, 3 times totaling 60 mL, to gain any residue thus E, E- dibenzalacentone was left in the round bottom flask. The dried solid was transferred to a pre-weighed Erlenmeyer flask where its mass was measured and the results were 0.65 g=65%. A small sample of the crude solid was taken and smeared on a microscope slide by use of the flat end of the micro spatula after which a melting p ...
Study of invitro anti-Oxidant Activity of Ipomea Pes-Caprae
The traditional medicinal plant ipomea pes- caprae belongs to convolvuceae family. The present study has been undertaken to find out the antioxidant activity of the whole plant extract of Ipomea pes-caprae. Plant was subjected to extraction by cold maceration by using ethanol as a solvent. Antioxidant activity such as 1,1-Diphenyl,2-Picryl,Hydrazyl (DPPH) Radical Scavenging Activity, Hydroxyl Radical Scavenging Activity, Reducing Power, Metal chelating activity were determined. Physicochemical analysis was carried out to identify the chemical constituent of the plant and showed the presence of alkaloid, sugar, steroid, glycoside, saponins, Terpenoids and phenol compounds. The result of free radical scavenging activity of EEIP by DPPH reduction revealed that the test compound is electron donor and could react with free radicals to convert them to more stable product and terminate radical chain reaction. For the measurement of reducing ability we investigated the Fe3+ to Fe2+ transformation. The metal chelating capacity of the EEIP and standard anti oxidants are determined by accessing the ability to complete with bipyridil and thiocyanate for Fe3+ and Fe2+ respectively. The formation of ferrous bipyridil, ferric – thiocyanate is not complete in the presence of EEIP. The ability of chelating is increased with increased concentration. So, it can be assumed that the plant extract chelate the iron. The experiment demonstrates that action of plant extract as per oxidation protector may be related to its iron binding ability.
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Synthesis of E, E-dibenzalacetone (E, E-DBA) Lab ReportN.docx
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Study of invitro anti-Oxidant Activity of Ipomea Pes-Caprae
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Phdhepatoprotective
- 1. Phytochemical investigation of all extracts was performed Petroleum ether fraction gave positive results for cardiac glycosides, steroids, tannins, phenolic compounds and flavonoids. Alcoholic fraction gave positive results for presence of carbohydrates, and tannins, flavonoids. Aqueous basic layer fraction also gave positive results for alkaloids, mucilages and flavonoids. On the basis of these results it was found that nearly all fractions were having phenols and tannins, important secondary metabolite of plant reported to possess antioxidant potential. Further DPPH assay of all extracts were performed. Petroleum ether fraction showed IC-50 57.62 µg/ml. Ethanol fraction showed IC-50 38.37 g/ml. H2O2 assay of petroleum ether and alcoholic extract showed IC-50 55.27 and 85.27 µg/ml respectively. OH-ion assay of petroleum ether and alcoholic extract showed IC-50 203.85 and 202.79 µg/ml respectively. Thus antioxidant assay revealed that the -------------------fraction was having best antioxidant activity and thus on the basis of this, it was decided that hepatoprotective activity of this fraction will be assessed.