Recombinent DNA Technology

1. An Assignment on-An Assignment on-
RECOMBINENT DNA TECHNOLOGYRECOMBINENT DNA TECHNOLOGY
Presented by_Presented by_

2. Deoxyribonucleic acid (DNA) is a long double-
stranded chain of nucleotides
Deoxyribonucleic acid (DNA) is a long double-
stranded chain of nucleotides
• DNA is made up of four nucleotides: A, C, G,
and T.
• A always pairs with T.
• C always pairs with G.
• The two strands of DNA are in an antiparallel
configuration.
• DNA is made up of four nucleotides: A, C, G,
and T.
• A always pairs with T.
• C always pairs with G.
• The two strands of DNA are in an antiparallel
configuration.
Recombinent DNA:Recombinent DNA:
Techniques in which DNA fragments are linked to self-
replicating vectors to create recombinant DNA molecules
which are replicated in a host cell.
Techniques in which DNA fragments are linked to self-
replicating vectors to create recombinant DNA molecules
which are replicated in a host cell.
Link: Link: http://en.wikipedia.org/wiki/Recombinant_DNALink: Link: http://en.wikipedia.org/wiki/Recombinant_DNA

3. Making recombinant
DNA:
Making recombinant
DNA:
Cut with restriction enzymesCut with restriction enzymes
Isolate DNAIsolate DNA
Ligate into cloning vectorLigate into cloning vector
Transform recombinant DNA
molecule into host cell
Transform recombinant DNA
molecule into host cell
Link: Link:
http://en.wikipedia.org/wiki/
Recombinant_DNA
Link: Link:
http://en.wikipedia.org/wiki/
Recombinant_DNA

4. Why is rDNA important?Why is rDNA important?
Better Crops (drought & heat resistance)
Recombinant Vaccines (ie. Hepatitis B)
 Prevention and cure of sickle cell anemia
Prevention and cure of cystic fibrosis
Production of clotting factors
Production of insulin
Production of recombinant pharmaceuticals
Plants that produce their own insecticides
Germ line and somatic gene therapy
Better Crops (drought & heat resistance)
Recombinant Vaccines (ie. Hepatitis B)
 Prevention and cure of sickle cell anemia
Prevention and cure of cystic fibrosis
Production of clotting factors
Production of insulin
Production of recombinant pharmaceuticals
Plants that produce their own insecticides
Germ line and somatic gene therapy

5. • Recombinant DNA is widely used in biotechnology,
medicine and research.
• Golden rice: a recombinant variety of rice that has been
engineered to express the enzymes responsible for β-
carotene biosynthesis.
• Herbicide-resistant crops.
• Insect-resistant crops.
• Recombinant Chymosin: Found in Rennet, is an enzyme
required to manufacture cheese.
• Recombinant DNA is widely used in biotechnology,
medicine and research.
• Golden rice: a recombinant variety of rice that has been
engineered to express the enzymes responsible for β-
carotene biosynthesis.
• Herbicide-resistant crops.
• Insect-resistant crops.
• Recombinant Chymosin: Found in Rennet, is an enzyme
required to manufacture cheese.
Link: Link: http://en.wikipedia.org/wiki/Recombinant_DNA

6. INSULIN for diabetics
FACTOR VIII for males suffering from hemophilia A
FACTOR IX for hemophilia B
Human growth hormone (HGH)
Erythropoietin (EPO) for treating anemia
Several types of ‘Interferons’
Several ‘Interleukins’
Granulocyte-macrophage colony-stimulating factor
(GM-CSF) for stimulating the bone marrow after a bone
marrow transplant
Parathyroid hormone
INSULIN for diabetics
FACTOR VIII for males suffering from hemophilia A
FACTOR IX for hemophilia B
Human growth hormone (HGH)
Erythropoietin (EPO) for treating anemia
Several types of ‘Interferons’
Several ‘Interleukins’
Granulocyte-macrophage colony-stimulating factor
(GM-CSF) for stimulating the bone marrow after a bone
marrow transplant
Parathyroid hormone
Link: Link: http://en.wikipedia.org/wiki/Recombinant_DNALink: Link: http://en.wikipedia.org/wiki/Recombinant_DNA