Genetic engineering and Transgenic

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Plant Genetic Engineering

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Genetic engineering and Transgenic

  1. 1. Genetic Engineering & Transgenic. Moses Okello Jr. MSc. (Ag. Biotechnology) Reg No. 0015 omoses48@yahoo.com
  2. 2. •Presentation Summary Introduction: Definition of G.E and History Item 1 Detailed process of Genetic Engineering & Transgenic formation. Item 2 Benefits of Genetic Engineering Item 3 Item 4 Item 5 Current Status citing Example & Statistics Future Prospects (R&D Pipeline)
  3. 3. Introduction Genetic Engineering The term refers to the manipulation of Plant genome to achieve a desired goal. Our major focus We must get the DNA: Into the cell Integrate into the genome Express (Everywhere or controlled)
  4. 4. Introduction Two main approaches for plant G.E Agrobacterium mediated gene transfer ( most commonly used method for Engineering crop plants) because of its ability to transfer DNA between itself and Plants Pioneered by J. Schell (Max Plank Inst., Cologne Direct gene transfer
  5. 5. History The history of manipulation dates back since man Began planting crops, about 10,000 years! It is even mentioned in the Bible. • Foreign genes were first introduced in plants in the Early 1980’s • 1983-first transgenic plant (Tobacco) Expressing Kanamycin resistance produced • 1986-first field trial for G.E plants established in France & U.S.A • 1994-Flavr Savr tomato approved for public, making it the first G.M whole plant commercialized •
  6. 6. •Cont • • 1995-Bt Potato approved safe by the environmental Protection agency.(first pesticide producing crop approved in the U.S) 2009-11 transgenic crops grown commercially in 25 countries
  7. 7. Genetic Engineering Process Agrobacterium mediated Plant Transformation • • • • • • • Isolation of gene of interest from a source organism Develop functional transgenic construct Insertion of transgene into ti-plasmid Introduction of the t-DNA containing plasmid into Agrobacterium Mixing of the transformed Agrobacterium with plant cells to allow transfer of t-DNA into the Plant Chromosome Regeneration of the transformed cells into GM Plant Testing for trait performance/transgene expression at the Laboratory, Green House, and Field trials
  8. 8. Genetic Engineering Process
  9. 9. Manipulation of Plant Genome
  10. 10. The Gene Gun
  11. 11. Applications          Insect-, pathogen-, and herbicide-resistant plants Stress- and senescence-tolerant plants Genetic manipulation of flower pigmentation Modification of plant nutritional content Modification of plant food taste and appearance Plant as bioreactors (Therapeutic agents, Antibodies) Edible vaccines Renewable energy crops Plant yield
  12. 12. Current Status of Transgenic Global Area under Cultivation
  13. 13. Future Prospects  Biofuel production will be the next greatest application of G.E for efficient biological production of bio
  14. 14. Thanks

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