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Transcription
• Tyler Campbell pd.7
DNA                    The whole step of transcription involves
                       an mRNA copy of the gene is made, it
                       carries the encoded information to the
      mRNA             cytoplasm.




             Nucleus




                   RNA polymerase-Reads DNA and build
                   the mRNA unit.
Promoter- turns the
gene on and off.
Coding region- This encodes the
information for the protein to
use.
Termination sequence-
signals the end of the
gene.
Now the promoter turns the genes on. It acts light
electricity and turns them on.
CC        Now the
                    GG          polymerase
            A       T           recognizes
            T       A           promoter
                    C
                    C           and
        T           A           produces
                  G G           protein.
        A             T
                      C
                   G G
    A                T
    T                A      A- adenine, T-
                  C C       Thymine= blue
    T                 A
                     GG     G- Guanine, C-
                            Cytosine= red
                    CC
                    CC
                    GG
    T                 A
                   C C
T                     A
A                     T
                   GG
                C       C
A                       T
                    C C
T                    A
CC
                          G G
                                  The DNA
                 A         T
                 T         A      polymerase
                                  binds to the
                          CC
                                  DNA and
             T             A
                                  double helix
                         G G      unwinds or
             A              T
                             C    unzips. It is
                          G G     transferring
         A                  T     the DNA
         T                 A      code into a
                         C C      mRNA code.
         T                   A
                            GG
                           CC
                          CC
                          GG           A
T                                          C
                         C
    T                                      A
     A                                      T
                         GG                    T
                     C
 A                                             C
T                            C     C
                              A
CC                  The
                          G G                    coding
                     A     u                     region
                     u     A
                                                 reache
                          CC                     s the
                 u         A                     single
                         G G                     strand
                 A          u                    and
                             C
                                                 builds
                          G G
             A                                   mRNA
                            u
             T             A                     copy
                         C C                     of it to
             u               A                   read.
                                  G
                                  G              The T
                                  CC             is now
                                       C         a U.
                          G            G
                                       A
     u                                   C
                                  C
                                        A
 u                                        u
A
                                         GG
         A                               C C
                                             u
                                        CC
C                    RNA polyme
                             C
                         G G                     reads the
                    A     u                      nucleotides
                    u     A                      decodes the
                         CC                      mRNA code
                          A                      nucleotides
                u
                                                 break free a
                        G G
                A          u                     turns into th
                            C                    double helix
                                   G G           again.
    A                                u
u                                   A
                                   C C
    u                                 A
                                    G G
                                      C C
                                 C   C
                                   G    G
        u                                  A
                                   C        C
        u                                   A
         A                                   u
                                  G        G
                                       C    C
            A                                u
                                      C     C
CC
                    GG      The
            A       u       termination
            u       A       sequence
                    C
                    C       comes in
        u           A       causes the
                  G G       mRNA strand
        A             u     to fall off of
                      C     the DNA
                   G G      strand to
    A                u      stop copying.
    u                A
                  C C
    u                 A
                     GG
                    CC
                    CC
                    GG
    u                 A
                   C C
u                     A
A                     u
                   GG
                C       C
A                       u
                    C C
u                    A
Translation
The mRNA strand is read and amino acids
are linked together into a protein at the first
part of translation.
The mRNA strand is read and amino acids
     are linked together into a protein at the first
     part of translation.
mRNA strand.
Now ribosomes read the
sequence of mRNA.
The ribosome links together
                  the amino acid also present
                  in the cytoplasm.




Amino acid pool
Now the individual nucleotides in the
                                          sequence are seen.


G C A A U U   G C G   G   A   U   U   A   C   G
The ribosome attaches to
                                                the mRNA strand and reads
                                                a codon at a time, (which is
                                                3 nucleotides).

A U G U U   G C G   G   A   U   U   A   C   G
A U G U U   G C G   G   A   U   U   A   C   G
A U G   G C G   G   A   U   U   A   C   G
A U G U U   G C G   G   A   U   U   A   C   G
A U G U U   G C G   G   A   U   U   A   C   G
A U G U U   G C G   G   A   U   U   A   C   G

                                                U A C




     tRNA
A U G U U    G C G   G   A   U   U   A   C   G

                                                 U A C




Amino acid
Anti codon


A U G U U        G C G   G   A   U   U   A   C   G

                                                     U A C




The amino acids
are transferred to
the ribosome by
tRNA molecules.
Anti codon


A U G U U       G C G   G   A   U   U   A   C   G




The anticodon of
tRNA dictates
which amino acid it
carries to the
ribosome.
The tRNA structure is coiled up in the   A U G U U    G C G   G   A   U   U   A   C   G
proper shape and now has read the
codes.

                                           UAC

                        UAA
                                                UGC




                                          GCC
The tRNA binds to the mRNA code.
            A U G U U        G C G   G     A   U   U   A   C   G
            U A C

                                         UAC

                      UAA
                                           UGC




                                     GCC
The tRNA binds to the mRNA code.
            A U G U U        G C G   G     A   U   U   A   C   G
            U A C, A A       C,

                                         UAC

                      UAA
                                           UGC




                                     GCC
The tRNA binds to the mRNA code.
             A U G U U G C G       G     A    U   U   A   C   G
          U A C, A A     C, G C    C,

                                        UAC

                   UAA
                                         UGC




                                   GCC
The tRNA binds to the mRNA code.
             A U G U U G C G       G     A   U   U    A   C   G
          U A C, A A     C, G C    C, U      A   A,

                                       UAC

                   UAA
                                         UGC




                                   GCC
The tRNA binds to the mRNA code.
             A U G U U G C G       G     A   U   U   A   C   G
          U A C, A A     C, G C    C, U      A   A, U    G   C

                                       UAC

                   UAA
                                         UGC




                                   GCC
During this process 2
A U G U U     G C G    G     A   U   U   A   C   G
                                                     amino acids come
U A C, A A    C, G C   C, U      A   A, U    G   C   together to form a
                                                     peptide bond.
                           UAC

      UAA
                             UGC




                       GCC
   Peptide bond
                                             Peptide bond
As the tRNA matches
A U G U U    G C G    G     A   U   U   A   C   G
                                                    each codon and
U A C, A A   C, G C   C, U      A   A, U    G   C   binds to the
                                                    ribosome, and the
                          UAC                       amino acids bond.


      UAA
                            UGC




                      GCC
This process
A U G U U    G C G    G     A   U   U   A   C   G
                                                    continues until it
U A C, A A   C, G C   C, U      A   A, U    G   C   reaches the end of
                                                    the gene.
                          UAC

      UAA
                            UGC




                      GCC
The last tRNA falls
A U G U U    G C G    G     A   U   U   A   C   G
                                                    off of the ribosome
U A C, A A   C, G C   C, U      A   A, U    G   C   and the ribosome
                                                    falls off of the mRNA
                          UAC                       strand.


      UAA
                            UGC




                      GCC
Now the amino acid chain folds into a 3-
dimensional figure ran by the order of amino
acids. This structure gives each protein its
own function.
The overall process of translation involves
ribosomes reading the mRNA’s code and
turning into tRNA’s code so it can make
protein.
The mRNA strand has bases on
it, the ribosome is reading
them.
The mRNA strand has bases on
      it, the ribosome is reading
      them.




AUG
The mRNA strand has bases on
            it, the ribosome is reading
            them.




AUU
      AUG
      UUA
The mRNA strand has bases on
                it, the ribosome is reading
                them.




GCA   AUU AUG
      CGA UUA
          AAG
The mRNA strand has bases on
                it, the ribosome is reading
                them.




          AUU
GCA AUG   AAG
GGG UUA   CGA
    UAU   AGC
The mRNA strand has bases on
                         it, the ribosome is reading
                         them.




AUG   AAA     UUA AUU
      UUU CCC GGG AAG
          CGG GCA CGA
               UAU AGC
          GGG      AAG
The termination sequence has
                         stopped the ribosomes from
                         reading them.




AUG   AAA     UUA AUU
      UUU CCC GGG AAG
          CGG GCA CGA
               UAU AGC
          GGG      AAG
Summary of steps
In transcription- The DNA code copy for you gets split in half from the DNA
polmerase and gets turned into a double helix again, the ribosome reads it and this
time has the code for an mRNA.
In translation- The mRNA strand is read and amino acids are put together to from a
protein and make a new code for tRNA from mrNA.
The process of translation and
transcription is done



           The End

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Transcription and Translation Process

  • 2. DNA The whole step of transcription involves an mRNA copy of the gene is made, it carries the encoded information to the mRNA cytoplasm. Nucleus RNA polymerase-Reads DNA and build the mRNA unit.
  • 4. Coding region- This encodes the information for the protein to use.
  • 6. Now the promoter turns the genes on. It acts light electricity and turns them on.
  • 7. CC Now the GG polymerase A T recognizes T A promoter C C and T A produces G G protein. A T C G G A T T A A- adenine, T- C C Thymine= blue T A GG G- Guanine, C- Cytosine= red CC CC GG T A C C T A A T GG C C A T C C T A
  • 8. CC G G The DNA A T T A polymerase binds to the CC DNA and T A double helix G G unwinds or A T C unzips. It is G G transferring A T the DNA T A code into a C C mRNA code. T A GG CC CC GG A T C C T A A T GG T C A C T C C A
  • 9. CC The G G coding A u region u A reache CC s the u A single G G strand A u and C builds G G A mRNA u T A copy C C of it to u A read. G G The T CC is now C a U. G G A u C C A u u A GG A C C u CC
  • 10. C RNA polyme C G G reads the A u nucleotides u A decodes the CC mRNA code A nucleotides u break free a G G A u turns into th C double helix G G again. A u u A C C u A G G C C C C G G u A C C u A A u G G C C A u C C
  • 11. CC GG The A u termination u A sequence C C comes in u A causes the G G mRNA strand A u to fall off of C the DNA G G strand to A u stop copying. u A C C u A GG CC CC GG u A C C u A A u GG C C A u C C u A
  • 13. The mRNA strand is read and amino acids are linked together into a protein at the first part of translation.
  • 14. The mRNA strand is read and amino acids are linked together into a protein at the first part of translation. mRNA strand.
  • 15. Now ribosomes read the sequence of mRNA.
  • 16. The ribosome links together the amino acid also present in the cytoplasm. Amino acid pool
  • 17. Now the individual nucleotides in the sequence are seen. G C A A U U G C G G A U U A C G
  • 18. The ribosome attaches to the mRNA strand and reads a codon at a time, (which is 3 nucleotides). A U G U U G C G G A U U A C G
  • 19. A U G U U G C G G A U U A C G
  • 20. A U G G C G G A U U A C G
  • 21. A U G U U G C G G A U U A C G
  • 22. A U G U U G C G G A U U A C G
  • 23. A U G U U G C G G A U U A C G U A C tRNA
  • 24. A U G U U G C G G A U U A C G U A C Amino acid
  • 25. Anti codon A U G U U G C G G A U U A C G U A C The amino acids are transferred to the ribosome by tRNA molecules.
  • 26. Anti codon A U G U U G C G G A U U A C G The anticodon of tRNA dictates which amino acid it carries to the ribosome.
  • 27. The tRNA structure is coiled up in the A U G U U G C G G A U U A C G proper shape and now has read the codes. UAC UAA UGC GCC
  • 28. The tRNA binds to the mRNA code. A U G U U G C G G A U U A C G U A C UAC UAA UGC GCC
  • 29. The tRNA binds to the mRNA code. A U G U U G C G G A U U A C G U A C, A A C, UAC UAA UGC GCC
  • 30. The tRNA binds to the mRNA code. A U G U U G C G G A U U A C G U A C, A A C, G C C, UAC UAA UGC GCC
  • 31. The tRNA binds to the mRNA code. A U G U U G C G G A U U A C G U A C, A A C, G C C, U A A, UAC UAA UGC GCC
  • 32. The tRNA binds to the mRNA code. A U G U U G C G G A U U A C G U A C, A A C, G C C, U A A, U G C UAC UAA UGC GCC
  • 33. During this process 2 A U G U U G C G G A U U A C G amino acids come U A C, A A C, G C C, U A A, U G C together to form a peptide bond. UAC UAA UGC GCC Peptide bond Peptide bond
  • 34. As the tRNA matches A U G U U G C G G A U U A C G each codon and U A C, A A C, G C C, U A A, U G C binds to the ribosome, and the UAC amino acids bond. UAA UGC GCC
  • 35. This process A U G U U G C G G A U U A C G continues until it U A C, A A C, G C C, U A A, U G C reaches the end of the gene. UAC UAA UGC GCC
  • 36. The last tRNA falls A U G U U G C G G A U U A C G off of the ribosome U A C, A A C, G C C, U A A, U G C and the ribosome falls off of the mRNA UAC strand. UAA UGC GCC
  • 37. Now the amino acid chain folds into a 3- dimensional figure ran by the order of amino acids. This structure gives each protein its own function.
  • 38. The overall process of translation involves ribosomes reading the mRNA’s code and turning into tRNA’s code so it can make protein.
  • 39. The mRNA strand has bases on it, the ribosome is reading them.
  • 40. The mRNA strand has bases on it, the ribosome is reading them. AUG
  • 41. The mRNA strand has bases on it, the ribosome is reading them. AUU AUG UUA
  • 42. The mRNA strand has bases on it, the ribosome is reading them. GCA AUU AUG CGA UUA AAG
  • 43. The mRNA strand has bases on it, the ribosome is reading them. AUU GCA AUG AAG GGG UUA CGA UAU AGC
  • 44. The mRNA strand has bases on it, the ribosome is reading them. AUG AAA UUA AUU UUU CCC GGG AAG CGG GCA CGA UAU AGC GGG AAG
  • 45. The termination sequence has stopped the ribosomes from reading them. AUG AAA UUA AUU UUU CCC GGG AAG CGG GCA CGA UAU AGC GGG AAG
  • 46. Summary of steps In transcription- The DNA code copy for you gets split in half from the DNA polmerase and gets turned into a double helix again, the ribosome reads it and this time has the code for an mRNA. In translation- The mRNA strand is read and amino acids are put together to from a protein and make a new code for tRNA from mrNA.
  • 47. The process of translation and transcription is done The End