serum and plasma

1. WINTER GREEN
ACADEMY
DHA Exam Preparation for
LAB TECHNOLOGISTS

2. SERUM & PLASMA

3. SERUM
Serum is blood plasma without fibrinogen or the
other clotting factors
Whole blood – cells and clotting factors = SERUM

5. PREPARATION
1. Take blood in a plain bottle
2. Keep the tube for 15 -30 min
3. Centrifugation: 1500 rpm for 10 min
4. Pale yellow colored serum obtained
Storage : 2-8 degree cel

6. PLASMA

7. PLASMA
The liquid /Fluid portion of blood
Light yellowish or Straw- colored fluid

8. PLASMA
PREPARATION
1. Take blood with specific anti-coagulant
2. Shake the tube for mixing ( delay in shaking
partial clotting of specimen occurs)
3. Centrifugation : 1500 rpm for 10 min
2-3 ml anti-coagulated blood  0.5ml plasma
obtained
5-6 ml blood  clot 1ml serum obtained
Storage : 2-8 Deg Cel

9. If PLASMA Value greater than SERUM value (%)
TPR ( Total Protein Rate ) 4%
LDH ( Lactate Dehydrogenase ) 2.7%
Chloride 0.2%
Calcium 0.9%

10. If no difference between SERUM & PLASMA
values
Bilirubin
Cholesterol
Creatinine

11. If PLASMA value less than SERUM value (%)
Glucose 5.1
Phosphorus 7
Potassium 8.4
CPK 2.1
Albumin 1.3
Alkaline phosphatase 1.6
Urea 0.6
Bicarbonate 1.8

12. CHANGES IN BLOOD ON KEEPING
Following changes occurs if Room temperature long
time (90-120 min)
Glycolysis  glucose converted to lactic acid
Formation of ammonia from urea (bacterial action)
Increased plasma inorganic phosphorus (formation
of ester phosphate in RBC)
Increased potassium
Conversion of pyruvic acid to lactic acid
Loss of carbon dioxide
Passage of bicarbonate from plasma to cells
Passage of chlorides from cell to plasma

13. THANK YOU