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DSM eBOOST, Industrially Proven Yeast Technology
1. CONFIDENTIAL
Advanced Yeast Technologies
Š 2020 DSM. All rights reserved.
Same Corn, More Value:
Industrially proven Yeast Technology
Phil Bureman
Sales Manager
DSM Bio-Products & Services
Phil.Bureman@DSM.com
Cell: +1 913-708-4969
September 11, 2020
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CONFIDENTIAL Confidential
Phil Buremanâ Sales Manager
⢠Started in biofuels industry in 1993
⢠25 yrs Technical Sales at Nalco with focus
on process chemicals and fuel quality
⢠10+yrs RFA Technical Committee
⢠Began with DSM in March 2020
Our Local Team â DSM Biobased Product & Services
Michael Mszal â Bioprocess and Mfg Specialist
⢠Started in biofuels industry in 2014
⢠8 years experience in production, R&D,
scale-up, and other roles in various
fermentation houses as a supervisor and
process development engineer
⢠Began with DSM in May 2016
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Pat Moran â Sales Manager
⢠Started in biofuels industry in 2005
⢠Held various positions with Walling
Water/ HOH Water, US Water including
General Manager/President, Regional
Sales Manager
⢠Began with DSM in August 2019
Who is DSM?
Arvind Kaul â Bioprocess and Mfg Specialist
⢠Started in biofuels industry in 2016
⢠Held technical positions with Roche
Vitamins, Novozymes, Genomatica, DSM
⢠Overall 25+ years of technical experience
in fermentation based processes.
⢠Began with DSM in late May 2016
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CONFIDENTIAL Confidential
Our purpose is to create brighter lives for people
today and generations to come
We connect our unique competences in Life Sciences and
Materials Sciences to create solutions that nourish,
protect and improve performance
DSM: A Global Innovation and Sustainability Leader â
#2 currently on Forbes Sustainability Index
2
Animal Nutrition
& Health
Human Nutrition
& Health;
Food Specialties;
Personal Care
Materials
Sustainability
Leader
Fortune Magazine:
2nd in âChange the
Worldâ index
Intrinsically
Innovative
21% sales from
innovation products
Global
presence
22,174
employees
worldwide
Strong
performance
$10 billion sales up
2%
from 2018
Other
PEOPLE â PLANET - PROFIT
What can DSM offer to your site?
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CONFIDENTIAL Confidential
DSM in biofuels 150 years of yeast experience
Vision: Accelerate the growth of biofuels as global fuel source
Mission: Deliver the technologies and expertise that enhance biofuel production
History: A rich history in ethanol production; began as Gist-Brocades producing yeast and
spirits since 1869
150 years
Gist-Brocades, NL Elgin, IL
We are a relative new comer in USA Bioethanol so we must Innovate!
Let me show you what we have created so far
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CONFIDENTIAL Confidential
Why Should You Consider DSM BP&S
⢠We are the newcomer to the USA Ethanol Industry
ďź So we have to innovate and work harder to earn and keep your business!
ďź DSM DOES NOT charge Technology or Ferm Fees â All In $/kg Pricing!
⢠DSM Yeast produces a Cleaner Ethanol!
ďź DSM Yeast delivers an average of 3% more ethanol compared to non GMO industry
standard with the same or lower amounts of acetaldehyde & acetal & other fusels
ďź Glycerol reduction (up to 68%)
ď§ Lower energy costs by increasing drying and evaporator efficiency
ď§ Improves whole stillage separation efficiency & corn oil recovery
ďź We believe our nitrogen uptake pathway converts more carbon to useful amino acids
& polypeptides thereby improving the quality of your DDGS
⢠We offer Monthly Lab Analysis, Quarterly Business Reviews & Regular Operator
Training. We are MORE than a delivery & an invoice!
5
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CONFIDENTIAL Confidential
⢠DSM Yeast has very good acid tolerances showing up to 0.5% LA + 0.2% AA with
negligible inhibition to finish
ďź DSM Yeast DO NOT contribute to the production of Acetic or Lactic acids
⢠eBoostTM GT offers high GA reductions (~50%) compared to traditional yeasts,
and further increases shown over other GA expressing yeasts
ďź A recent mini-ferm trial showed additional 25% GA reduction
⢠eBOOST⢠& eBOOST⢠GT are produced in the U.S. / All regulatory approvals in
place (MCAN*, GRAS)
⢠At this time we offer only Active Dry Yeast (ADY)
6
I can share some Performance Data but first
Letâs talk about what is important to you
*Microbial Commercial Activity Notices (MCANs)
Why Should You Consider DSM BP&S
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CONFIDENTIAL Confidential
7
Letâs focus on your interest
⢠What is most important to you when choosing a yeast supplier?
⢠What are your expectations from a yeast supplier?
⢠What do you wish a yeast supplier would offer or innovate?
⢠Other thoughts/questions/coaching for us?
ďź Are you making or considering making hand sanitizer?
⢠Do you have an ability to test our yeast in a mini ferm or lab trial?
ďź If so, do you place much value is such tests?
⢠Is there an appetite now to trial one or both of our yeast?
ďź Have you had challenges optimizing your plant to a new yeast in the past?
ďź If so, what expectations do you have for DSM in a potential trial or switch?
ďź We are offering very low cost trials at this time!
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CONFIDENTIAL Confidential
DSM eBOOST yields CLEAN Ethanol
Lab Test Measurements in Fermentation Broth
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Mash Yeast
Acetaldehyde
(mg/l) % of ER
Corn
Mash #1 Ethanol Red 77.5 100
Corn
Mash #1 eBOOST⢠65.9 85
Corn
Mash #2 Ethanol Red 101 100
Corn
Mash #2 eBOOST⢠73.6 73
Mash Yeast
(Acetal) 1,1-dieth
oxyethane (mg/l) % of ER
Corn Mash
#1
Ethanol
Red 6.77 100
Corn Mash
#1 eBOOST⢠7.56 112
Corn Mash
#2
Ethanol
Red 10.9 100
Corn Mash
#2 eBOOST⢠8.21 75
⢠Temperature Profile for this testing was
SOF to 22 Hrs: @ 94 F
22 Hrs to 36 Hrs @ 92.5 F
36 Hrs to 69 Hrs (EOF) @ 89.6 F
⢠Measurements were done from fermentation drop
samples
⢠Drop samples were centrifuged and supernatants
collected and frozen
⢠Samples sent to a certified third-party analytical lab
for acetaldehyde and 1,1-Diethoxyethane (acetal)
analysis by GC
Letâs talk Commercial Results
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CONFIDENTIAL Confidential
Commercial trial results with
Same corn, more value
eBOOST⢠compared to market standard:
⢠About 3% avgerage EtOH yield improvement
⢠Down to 68% glycerol reduction
32%
45%
38%
50%
40% 41%
38%
41%
0%
20%
40%
60%
80%
100%
Market
standard
Plant 1 Plant 2 Plant 3 Plant 4 Plant 5 Plant 6 Plant 7 Average
Low glycerol levels eBOOSTâ˘
2.0%
5.0%
4.7%
3.5%
2.1%
1.9%
2.5%
3.1%
98%
100%
102%
104%
106%
Market
standard
Plant 1 Plant 2 Plant 3 Plant 4 Plant 5 Plant 6 Plant 7 Average
EtOH yield improvement eBOOSTâ˘
Equal or very similar performance observed with eBOOST⢠GT
In todayâs low price market your
plant could make the same ethanol
with less corn input cost!
How does DSM compare with other
Market Leaders?
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CONFIDENTIAL Confidential
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Outperforms competitors
Best yield potential in the market
2.5 - 5%
50 - 60%
Equal or very similar performance observed with eBOOST⢠GT
Let me share details on in Plant Performance Data for
eBoost GT Vs a Competitor Advanced Yeast
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CONFIDENTIAL Confidential
Charts at Fermentation drop â Trial-O-Rama
eBOOST⢠delivers higher EtOH and EtOH/Liq solids at drop
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Š 2019 DSM. All rights reserved.
ďeBOOST⢠delivers consistently higher EtOH titers and yields at drop vs. the
other two strains.
Compt 1 - Baseline Compt 2
eBOOSTâ˘
Compt 2
eBOOSTâ˘
Ethanol
Ethanol/Liq solids
Compt 1 â Re-baseline
Compt 1 - Baseline Compt 1 â Re-baseline
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CONFIDENTIAL Confidential
eBOOST⢠GT Recipe â Key Operating Conditions
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⢠Propagator
â Liq solids ~ 20%; Yeast Pitch â 1 g/l with opportunity for optimization.
â Urea ~ 1 g/l
â No GA
â Propagation time of ~ 8-9 hrs
â Temperature: 90-93 F
⢠Main Fermenter
â Mash solids of 32-34%
â Urea ~1.0- 0.8 g/l
â 50% of normal GA dosage
â Plant standard temperature staging 92 â 88 F targeted ranges;
â In sustained commercial use, eBOOST GT had good finish with high ethanol titers even when
fermentation temperatures occasionally peaked at 95F
First, let me share some sugar
consumption data vs a
competitor using this recipe
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CONFIDENTIAL Confidential
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Evaluation of performance â eBOOST GT vs. Comp Yeast @ Recent Ferm Trial
⢠This data is @ a 50% reduction in GA which can be optimized up to
70%.
⢠eBOOST GT has a higher tolerance for glucose peak compared to
competitor yeast
⢠eBOOST⢠GT produces less Glycerol during fermentation
⢠No significant difference in lactic and acetic acid production
compared to current plant levels.
Equal or very similar performance observed with eBOOSTâ˘