14. Parallel Streams of Research
CAN be Engineered
Synthetic Biology
Bioluminescent bacteria
Development of synthetic Luciferin
Vibrio Fischeri animation
CANNOT be Engineered
Focus on optimizing growth conditions
Study on lighting patterns to improve collective flashing
Bioluminescent Dinoflagellates
14
17. Bioluminescence as solution..
Why is Bioluminescence special ?
Cold light
Most efficient of all the sources of lights. ~98% efficiency
As cultured at micro-organic level, immune to external impacts
No environmental impact
Once genetically re-engineered, unlimited source
“Grow your own light“
17
20. InVivo Imaging
70% 36% 32% 25% 18%
Oncology/Cancer Inflammatory
Diseases
Neurology Cardiovascular Drug Metabolism
Studies
What is InVivo Imaging ?
A noninvasive insight into living organisms
Understand disease related changes in the body
Detection of key Diseases
OtherApplication
68% 59% 49% 41% 24%
Monitoring Biodistribution Cancer cell Biomarkets Structural Imaging
Treatment ResponseSource : Drug Discovery World S
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1
1
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In vivo preclinical imaging Layout 1 23/06/2011
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21. InVivo Imaging
Magnetic Resonance Imaging (MRI)
Single photon emission computed
tomography (SPECT)
Positron emission tomography (PET)
Conventional Methods
MRI
SPECT
PET
Optical Imaging – Bio Luminescence Imaging
Optical,
28% MRI, 23%
PET
, 20%
Others,
26%
Optical
SPECT, 3%
MRI PET SPECT Others
Source : Drug Discovery World Summer 2011, In vivo preclinical imaging Layout 1 23/06/2011
14:05 Page 59
22. BLI - Concept
Growth of cancer cell over 4 weeks can be observed
Source : A New Diagnostic System in Cancer Research: Bioluminescent Imaging (BLI)*, Z., Ralph MASON, Peter ANTICH,
Edmond RICHER, Woodring E. WRIGHT
23. PET vs.MRI vs.BLI
Equipment Costs
Mouse Preparation
Time
Scanning Time
Total Time for
1 animal
Data Analysis
Total Time for
10 animal
>600 K USD 1-2 million USD <500 K USD
1hr 30 min 30 min 20 min
15min / 3D Scan 30 min / 2D Scan 1s – 2min
Experts Required Experts Required Straight Forward
1 hr 1hr 30min 20min
>600 K USD 1-2 million USD <500 K USD
Data Analysis Time
>600 K USD 1-2 million USD <500 K USD
PET MRI BLI
Source : A Comparison of Imaging Techniques to Monitor Tumor Growth and Cancer Progression in Living Animals, Anne-
Laure Puaux,Lai Chun Ong,Yi Jin,Irvin Teh,Michelle Hong,Pierce K. H. Chow, Xavier Golay
25. Applications in the Food Industry
VALUE
PROPOSITION
Disposable
Low cost
Easy operation
Fast response
Prototype ofATP bioluminescence based Biosensor for detection of
bacteria
Source : Disposable bioluminescence-based biosensor for detection of bacterial count in food, Analytical Biochemistry 394 (2009) 1-6
26. Opportunities in the Food Industry
In alocal butcher shopsfor detection of contaminated food substancessuch
asmeat.
To quickly and easily detect if small individual samples (i.e.per bottle) of
fluids, suchasmilk or water, havebeencontaminated/spoiled during
packaging transportation.
In remote areas such asSaharan desert andAlaska.
Can be used by rescue workers during natural disasters such asearthquakes
and tsunamis.
Future Space missions (e.g.during along mars mission)
27. Genetically engineered Bioluminescent pets
Source : http://www.ibtimes.com/glowing-dark-rare-pictures-genetically-engineered-fluorescent-animals-photos-840089
Genetically engineeredAngelfish (Pterophyllum Scalare) glow in atank under ablack light while being
displayed at the 2010Taiwan International Aqua Expo inTaipei October 29,2010.
28. Bioluminescence Why does Bioluminescence occur?
How it Happens ?
BioluminescenceVs. FluorescenceVs.
Incandescence
Opportunities Lighting
Inferences and
Conclusion
Biomedical Imaging
Food Industry
Commercialization
Entrepreneurial O pportunities
Technology Convergence
Conclusion
Outline
51
29. Factors for commercialization
Maturity of technology
Scalability
Entrepreneurial opportunities
Market penetration
Need for acheaper lighting
Cost effectiveness
Food
Contamination
Lighting BLI
53
Other
new
areas
of
market
penetration
30. Conclusion
Changeprovides opportunities
Bioluminescence provides anew dimension to Lighting,Healthcare and Food
industry
Adoption of this technologies will lead to massive growth Bioluminescence
When technology matures andbecomeseconomically feasible, it will definitely
offer asuperior value proposition
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