1. The overall procedure depicted in present study a flow chart is given below
Water Galing river as samples will collected using sterile glass bottles with
methods Depth Integrated Sampleat station Kilang Jaya Gading, Kawasan Setali,
Jalan Galing 23 & 33 and Vistana in Galing river, Kuantan
Isolation of bacteria from water sample
Water Galing river diluted from
10-1, 10-6, 10-8 dilution factor
Identification of water Galing river :
100 µl from each dilution, inoculated
into petri dished containing nutrient
agar
Accounting of
colonies
Incubated of petri
dished at 37 ˚C for 24 h
pH, temperature, conductivity, TDS,
salinity,OD (Oxygen Demand), TSS
(Total suspended solids), turbidity,
COD (Chemical oxygen demand),
BOD (Biochemical oxygen demand),
SS (Suspended solids), Iron (Fe),
chloride, NH4 (Nitrogen Ammonia),
Phosphorus, T. Phosphorus, sulphate.
Cultivation and morphological of bacteria
Successful of bacteria isolation,
then sub-cultured in petri dish
Identificated isolated of bacteria
with gram straining procedure
Classification of bacteria by
Bergey’s Manual determinative
bacteriology
Growth bacteria in culture medium
Take amount of bacteria from subculture, then put into flask
contained nutrient broth
Incubation of sample in
incubator shaker at 37 ˚C
until 24 h
Incubation all of sample at 37 ˚C until
24 h. Counting number of bacteria in
plate counter or see in UV lamp
The sample with growth
bacteria showed by turbid
color in the sample
Add each 1 ml sample with turbid color
into 3 petri dish containing phenol red,
rodamine B, twin 20, respectively
Batch Production
Prepare of flask 100 ml, add bacteria and medium
culture with comparison 10%; 20%; 30%; 40%; 50%.
Media culture namely: lipid, phenol, pesticides, and pb
Measuring by spectrometer
to know grow of bacteria
1

2. 8. Time Schedule
Year 1
No
Project Activities
Year 2
Sep Oct Nov Dec Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Jan Feb Mar Apr May Jun Jul Aug
1 Literature review
Collection sample water
Galing river in at station
Kilang Jaya Gading,
2
Kawasan Setali, Jalan
Galing 23 & 33 and Vistana
in Galing river, Kuantan
3
Characterization of water
Galing river
Media preparation and
4 Isolation of bacteria from
water Galing river
Cultivation and
morphological
5
characteristics of bacterial
isolates
6
Growth bacteria in culture
medium
Characterization of bacteri
7 using PCR and DNA, FTIR
and GC
8
Application Bacteria in
Batch Production
9 Publication/ report writing
Milestone
2

3. 3