Decoloration of Direct Red- Azo Dyes by Acinetobacter Jenuii

1. Decolorization of Direct Red (azodyes)
by Acinetobacter jenuii
Abstract
Azodyes are cheap, stable, accessible, give a variety of colours and are easy to produce but in the process of dying they are 10-50% unused and are
wasted in environment where they can cause severe environmental problems by causing water pollution. Of all the methods used to decolorize the
azodyes we have used the decolorization by bacteria through azoreductase which have several advantages and can effectively reduce the sludge.
However further studies are needed as the bacteria used can have serious health problems.
Introduction
They are:
● Electron withdrawing groups making
molecule resistant to degradation
● causing environmental problems such as
obstruction of light and oxygen
● some degradation intermediates are
virulent (cause mutation)
Methodology
First of all culture the bacteria in the medium.
Then add the bacteria to the solution of
azodye and measure optical density to check
bacterial activity and then centrifuge the
solution. the supernatant is used as
extracellular enzyme and the pellet is
suspended in a buffer and DNase is added in
it. Samples are incubated for 20 minutes. The
lysate is clarified by centrifugation at 10,000
rpm for 30 minutes at 40C and the pallets are
discarded.
Results:
Growth of Acinetbacter jenuii
Results: Optical Density
Protein Estimation Enzyme Assay
Conclusion
Experiments show that Acinetobacter jenuii can decolourize direct red at low cost also reducing amount of sludge considerably but still more work on
gene identification is required as Acinetobacter jenuii is observed causing pathological conditions in human.
Acknowledgement: This scientific work has been done with the aid of Pakistan Science Foundation and the experiments have been performed
in Government College University Faialabad.
Why Bacteria?
1. Fungal systems appear to be the most appropriate in
the treatment of colored and metallic effluents but it is
not feasible because of their long reproductive spans
2. Recently some studies describe the use of plants for
the dye removal from wastewater, however
phytoremediation presently faces a number of
obstacles including the level of pollutants tolerated by
the plant, the bioavailable fraction of the contaminants
and evapotranspiration of volatile organic pollutants as
well as requiring big areas to implant the treatment.
Muhammad Abdullah Nabeel, Muhammad Zain Ahmad Noor,
Dr. Habib Ullah Nadeem✱