Authentic knowledge.

1. Micropropagation
(In-Vitro Multiplication)
M.PHOOL BADSHAH

2. Define:
“ It is the process of quickly multiplying
stock plant material to produce a
large number of progeny plants, using
modern plant tissue culture
methods.”
• Usually used for Ferns, Orchids, many other
plants.

3. Clone & Clonal Propagation
• Clone is plant population derived from a
single individual by asexual reproduction.
• Clonal propagation is the multiplication
of genetically identical individuals by asexual
reproduction.

4. Key Role of Micropropagation
• Plants which are produced can be diseased
free.
• Production of unlimited number of clones,
multiplication stage can be recycled many
times.
• Clonal Reproduction Occurs.
• Easy to manipulate production cycles.

5. Kind of Plant Material Selected
• Genotype role
• Size of Explant required.
• Position on Plant Specie.
• Part of Plant Excised.
• Seasonal Effect
• Physiological Impact

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• In-vitro Propagation of Plant Species:
 Obtained from Cells, Tissues and Organs.
 Culture vessels containing.
 Cultured aseptically on defined media.
 Provide proper condition of Temperature & Light

7. Stages of Micropropagation
• Stage 1:
 Selection of Mother Plant (Explant)
 Sterilization of Plant Part
 Nutrient medium
• Stage 2:
 Shoot hormone producing shoots.
• Stage 3:
 Root hormone producing roots.
 Plant returned to soil pot.

8. Micropropagation Process & Stages

9. Method of Propagation
• Organogenesis
• Embryogenesis
• Micro Excision

10. Advantages
• Multiplication of explant in controlled Lab
conditions.
• Once established, inexpensive per plant.
• Disease free plant propagation occurs.
• Multiplication convert into one to many
propagules.

11. Disadvantages
• Initially expensive process but later
inexpensive.
• Some plants do not fulfill industrial
requirements.
• Special equipment in Lab needed.
• Without aseptic conditions, variations occurs.