Stem Cell Application

1. Stem cells
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Yasmine Hosny
Teaching assistant-Faculty of Science-Ain Shams University
CELL Team Board Member
Yasmine Hosny Hassan

2. 1. Hematopoietic stem cells (HSCs).
2. Isolation of HSCs.
3. Sub-culturing and counting of stem cells.
4. Applications of stem cells.
Agenda …
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3. Hematopoietic stem cells (HSCs)
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stem cells that give rise to other blood cells. This process
is called haematopoiesis. This process occurs in the red
bone marrow.
more than 500 billion blood cells are produced every day.
HSCs give rise to both the myeloid and lymphoid lineages
of blood cells.
Myeloid cells include monocytes, macrophages,
neutrophils, basophils, eosinophils, erythrocytes, and
megakaryocytes to platelets.
Lymphoid cells include T cells, B cells, and natural killer
cells. Yasmine Hosny Hassan

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5. Location
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Bone marrow
Umbilical Cord blood
Peripheral blood
Yasmine Hosny Hassan

6. Hematopoietic stem cells (HSCs)
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• https://drive.google.com/open?id=1CqvLj5ZU3qNo
EvIipz4Q1pr0uZyfWhcz
Cord blood collection

9. HSCs isolation
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HSCs isolation
• https://drive.google.com/open?id=16DcGTSx 11-
O0 dY2YewbOditI2yJctZ-P

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12. Detection of HSCs
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The cultured HSCs express on their surface CD34,
CD38, CD90, CD133, CD105.
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13. Subculture of stem cells
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14. Counting stem cells
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15. https://drive.google.com/open?id=1nSeT
PDX_4Figb3dUjLzRvnRXWdZMxUeX
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counting

16. Applications of stem cells
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18. Applications
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19. Hematopoietic stem cell transplant
Intense chemotherapy damages a patient’s bone
marrow where the stem cells for blood resides.
This left the patient vulnerable to infection, anemia
and bleeding.
These side effects of chemotherapy can be treated
with a bone marrow transplant.
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• https://drive.google.com/open?id=1awbUG4ZRgeIv
wQXze_cPUcEOWRT-sUZq
Baldness

22. stem cells and diabetes
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23. Stem cells and myocardial infraction
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24. Stem cells and myocardial infaraction
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25. Stem cells and myocardial infaraction
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• https://drive.google.com/open?id=11qxS-
Al2Qz72QqaMVR90UEtEgMWKI6sR
Heart

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30. Thank you for
your attention!
Any Questions?
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