how to present your future interest

1. MESELE TILAHUN BELETE (BSc. )
Assistant researcher (Amhara Agricultural Research Institute,
Bahir Dar, Ethiopia)
(meseletilahun1989@gmail.com /
mesytila.biot@gmail.com)
Tell +251583201938
Fax +2519582266077
Cell +251921637933
Skype Mesele Tilahun
Bahir Dar, Ethiopia
November 2019
Brief admission interview presentation to
University of Science and Technology, Korea Research
Institute of Bioscience and Biotechnology (KRIBB),
Integrative program

2. Contents
• Brief personal profile
• Education
• Experience
• Attended Training
• Developed Skill
• Previous Research
• Study plan
• Further Interests
• Reference

3. Brief personal profile
Name Mesele Tilahun Belete
Date of Birth 1989/4/27
Place of Birth Gondar, Ethiopia
Marital Status Single
Nationality Ethiopian

4. EDUCATION
July 2012 University of Gondar,
Ethiopia
Bachelor of Science in
Biotechnology
Jun 2009 Mekane Eyesus
preparatory school,
Ethiopia
Ethiopian Higher Education
Entrance Qualification
Certificate
Jun 2007 Mekane Eyesus
Secondary School,
Ethiopia
General Secondary School
Education Certificate

5. EXPERIENCE
Dec 2018 – present Assistant researcher II at Amhara Agricultural
Research Institute, Ethiopia
Dec 2016 – Dec 2018 Assistant researcher I at Amhara Agricultural
Research Institute, Ethiopia
Nov 2014 – Dec 2016 Junior researcher at Amhara Agricultural
Research Institute, Ethiopia
Mar 2013 – Nov 2014 Crop expert at Estie Distriict Agricultural
office, Ethiopia

6. ATTENDED TRAININGS
• “Early Generation Potato(G0) multiplication ( in-vitro & ex vitro): Principles,
Techniques and Application”, organized by Amhara Agricultural Research
Institute & World Vision Ethiopia Seed Potato Project from -Sept. 16-25, 2019,
Bahir Dar - Ethiopia
• “Molecular biology & Bioinformatics”, from Jun 3rd – 6th, 2019 by Institute of
Biotechnology at University of Gondar, Gondar -Ethiopia.
• “Molecular biology and Bioinformatics” from 31th May -3rd June 2017 by Dept.
of Biotechnology at University of Gondar, Gondar - Ethiopia.
• “Principles of Micro propagation in Agro-Biotechnology Training” organized
by Better Potato for Better Life (BPBL) Project of CIP from Nov. 24- 26, 2015,
Bahir Dar –Ethiopia.
• “Optimization of Protocol for Micro Propagation of Rosa Damascene” during
the 22nd Annual Research Conference of University of Gondar from Jun. 22-23,
2012, Gondar-Ethiopia.

7. DEVELOPED SKILL
• DNA isolation ( genomic & plasmid DNA )
• DNA quantification and confirmation using electrophoresis,
spectrophotometer and photometer/Nano Drop
• Preparation of competent cell and transformation with recombinant plasmid
by chemical methods using divalent and multivalent cations (Ca++ & Mg++)
• Operation of molecular techniques of equipments such as PCR,
Centrifuge, Gel Documentation system, and Micro plate reader
• Diagnosis of endophytic Bacterial wilt and potato virus (PVA, PVM, PVS,
PVX, PVY, PLRV) with Serological methods of NCM and DAS-ELISA
techniques
• Computer related skills: MS,PPT, excel, SAS, & R-studio

8. PREVIOUS RESEARCH (1)
BELETE Mesele Tilahun, ALEMU Gashaw Belay, Tadele Hulubanchi
(2019). In vitro Micro propagation of Ginger (Zingiber officinale
Rosc.). Manuscript in preparation, at Amhara Agricultural Research
Institute, Ethiopia.
ALEMU Gashaw Belay, BELETE Mesele Tilahun, MEKONEN Desalegn
Abebe, Tadele Hulubanchi. (2017). Introduction, Invitro screening,
disease diagnosis and initiation of Potato (Solanum tuberesum) to tissue
culture mass propagation. Manuscript in preparation, at Amhara
Agricultural Research Institute, Ethiopia.
ALEMEU Gashaw Belay, BELETE Mesele Tilahun, MEKONEN
Desalegn Abebe (2017). The effect of chitosan application on in vitro
and ex vitro plantlet quality and growth of potato(Solanum tuberosum).
Unpublished manuscript, at Amhara Agricultural Research Institute,
Ethiopia.
BELETE Mesele Tilahun, ALEMU Gashaw Belay, MEKONEN
Desalegn Abebe (2017). Determination of appropriate ex vitro
acclimatization media of potato micro-propagation. Unpublished
manuscript, at Amhara Agricultural Research Institute, Ethiopia.

9. PREVIOUS RESEARCH (2)
BELETE Mesele Tilahun and MEKONEN Desalegn Abebe (2016).
Adoption and Optimization of in vitro propagation protocol of Sweet
potato. Unpublished manuscript, at Amhara Agricultural Research
Institute, Ethiopia.
BELETE Mesele Tilahun (2012). In vitro micro propagation optimization
protocols of Rosa damascene (BSc. thesis). Unpublished manuscript,
University of Gondar, Ethiopia.
• Our institute priority is to scale up and implement research results to end users
such as to farmers, private & commercial Tissue Culture (TC) rather than
trying to publish them. As a result, the above research results have not yet been
published.

10. 1. Proposed study plan
Title: Study on Biocontrol Mechanism of Endophytic Bacterial wilt
(Ralstonia solanacearum) using morphological, biochemical, and
molecular techniques.
STUDY PLAN

11. 1.1 INTRODUCTION
• Bacterial wilt is one of the major diseases of tomato and
other solanaceous plants.
• The disease is caused by the bacterium Ralstonia solanacearum,
previously known as Pseudomonas solanacearum which is most
damaging plant pathogens.
• Strains of this pathogen affect more than 200 plant species in over
50 families throughout the world like Potato , tomato banana,
ginger , tobacco , sweet pepper, olive, rose and soybean etc (Rieter
et al.2012; Das et al. 2017; El-deeb et al. 2013 and Elbeltagy et al
2000).
• Endophytic bacteria may have certain metabolic properties, such as
promoting plant growth, controlling soil-borne pathogens, or
helping host plant to defeat stress responses to environmental abuse.

12. INTRODUCTION…(2)
• Biocontrol of bacterial wilt pathogen of tomato using endophytic
bacteria is one of control method to support sustainable agriculture
(NAWANGSIH et al. 2011).
• Several groups of bacteria have been explored for biocontrol of
pathogens. Plant Growth-Promoting Rhizobacteria (PGPR) are one
of the most studied biocontrol agents.
• However, PGPR are usually effective at laboratory or green house
scale, often fail to provide effective disease suppression in a field.
Therefore, this study focus on obtaining comprehensive pictures of
another group of endophytic bacteria from the tomato plants that
used as a biocontrol agents.

13. 1.2 OBJECTIVES
1.2.1 General Objectives
To Study the Biocontrol Mechanism of Endophytic Bacteria based on
morphological, biochemical, and molecular techniques
1.2.2 Specific objectives
• To select & characterize endophytic bacteria isolated from tomato stems,
and
• To investigate whether they can act as plant growth-promoter or as
biocontrol agents of tomato bacterial wilt disease.

14. 1.3 METHODOLOGY
• First Isolate and purify endophytic bacteria by
growing on Nutrient Agar (NA) from tomato
plants with no apparent disease symptoms.
• Then the Pathogenicity of Endophytic Bacteria
will be test by Inoculation of other host plants.
• Screening of endophytic bacteria for antibiosis
using in-vitro competition mechanism of culture
assay
• Finally Characterization & Identification Selected
Endophytic Bacteria will be analyzed based on
morphological (microscope and colony
appearances) biochemical properties, and
molecular analyses (Extracting DNA and
comparing sequence analysis of their 16S rRNA
gene)
Isolate and purify endophytic
bacteria
Inoculation of other
host plants
Screening of endophytic
bacteria for antibiosis
Characterization &
Identification
Fig. 1 Schematic procedure
Nutrient Agar
(NA)

15. Furthermore, I have commitments and interest to work on collaborative
research projects in my field such as
• Diversity of plant viruses in plant ecosystem,
• Functional analysis of plant (soybean) genes by molecular reverse genet
ics
• Drug delivery systems by icosahedral plant viruses as bionanoparticles.
FURTHER INTERESTS

16. REFERENCE
1. Mr.Gashaw Belay (MSc. )
Position- Case team coordinator of Biotechnology
Address- Amhara Agricultural Research Institute in Bahir Dar, Ethiopia
Phone +251-583201343
Fax +251-582266077
E-mail -gashaw.belay3@gmail.com
2. Menale Wonde (Ph.D. )
Position- Senior Researcher, Forestry research directorate of director
Address- Amhara Agricultural Research Institute in Bahir Dar, Ethiopia
Phone +251-918142623
Fax +251-582266077
E-mail –menalewondie@yahoo.com
3. Berhanu Andualem (Ph.D.)
Position-Professor, Instructor and researcher
Address- Institute of Biotechnology, University of Gondar, Ethiopia
Phone +251-918700027
Fax +251-581141240
E-mail - dberhanu2010@gmail.com

17. THANK YOU