Bioreactors for plant cell cultures


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Bioreactors for plant cell cultures

  1. 1. Bio-reactors for Plant Cell Culture 1
  2. 2. BIOREACTORS • A bioreactor is a vessel in which a chemical process is carried out which involves organisms or biochemically active substances derived from such organisms. 2
  3. 3. Types of cell cultures • Continuous culture • Batch culture • Immobilized cell lines 3
  4. 4. Types of Bio-reactors Used in Plant Cultures • Mechanically agitated bioreactors • Pneumatically driven bioreactors • Hydro dynamically driven disposable bioreactors • Non-Agitated bioreactor 4
  5. 5. Mechanically agitated bioreactors 5 Stirred tank reactors Rotary Drum bioreactors
  6. 6. Pneumatically driven bioreactors 6 Bubble column bioreactor Propeller loop airlift Jet loop airlift
  7. 7. Internal and external loop airlift 7
  8. 8. Disposable bioreactors • Disposable bioreactors represent modern alternatives to such traditional cultivation systems.These bioreactors consist of a sterile plastic chamber that is partially filled with media(10% to 55%) , inoculated with cells and discarded after harvest. • The single use chamber eliminating any need for cleaning or sterilization is made of FDA-approval biocompatible plastic such as polyethylene, polystyrene and polypropylene. Usually, the disposable bioreactors are low cost, simple to operate and guarantee high process security 8
  9. 9. Hydro dynamically driven disposable bioreactor 9 Wave and undertow bioreactors Biowave bioreactor
  10. 10. Non agitated bioreactors • Various types of membrane reactor were configured namely Hollow fiber reactor,Tubular membrane reactor, Flat plate membrane reactor with one side or two side flow, Multimembrane reactor • Fluidized bed reactor 10
  11. 11. Uses and advantages • These bioreactors are mostly used in suspension culture for large scale production of secondary metabolites. • They are used in biotransformation of compounds • Reduces labour • More utilization in pharmaceutical industry • More successful biomass production than the use of plant as bio factories 11
  12. 12. Disadvantage • Instability of the productive cell lines, • The slowness of the cell growth and production phase • Limited knowledge about the secondary metabolite pathways. 12
  13. 13. Thank you 13