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Photosynthesis: Life   from   Light,  Air & Water
Energy needs of life ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Energy needs of life ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
How are they connected? Heterotrophs Autotrophs making energy & organic molecules from light energy making energy & organic molecules from ingesting organic molecules Where ’ s  the  ATP? exergonic endergonic glucose  +  oxygen    carbon  +  water  +  energy dioxide C 6 H 12 O 6 6O 2 6CO 2 6H 2 O ATP  + + + +  water  +  energy    glucose  +  oxygen carbon dioxide 6CO 2 6H 2 O C 6 H 12 O 6 6O 2 light energy  + + +
Energy cycle Photosynthesis Cellular Respiration O 2 CO 2 plants animals ,  plants ATP The Great Circle of Life,AICE Kiddies H 2 O sun glucose
What does it mean to be a  plant ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],ATP CO 2 N P K … H 2 O glucose
Plant structure  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
stomate transpiration
Chloroplasts Chloroplasts Leaf Leaf Chloroplast absorb sunlight & CO 2 make energy & sugar Chloroplasts contain Chlorophyll CO 2
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Plant structure  H + H + H + H + H + H + H + H + H + H + H +
Photosynthesis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],It ’ s the  Dark Reactions !
Light Reactions O 2 H 2 O Energy Building Reactions ATP ,[object Object],[object Object],[object Object],[object Object],NADPH sunlight H 2 O ATP O 2 light energy  + + + NADPH
Calvin Cycle sugars C 6 H 12 O 6 CO 2 Sugar Building Reactions ADP ,[object Object],[object Object],[object Object],ATP NADPH NADP CO 2 C 6 H 12 O 6  + + + NADP ATP + NADPH ADP
Putting it all together Sugar Building Reactions Energy  Building Reactions ,[object Object],[object Object],[object Object],[object Object],O 2 H 2 O sugars C 6 H 12 O 6 CO 2 ADP ATP NADPH NADP CO 2 H 2 O C 6 H 12 O 6 O 2 light energy  + + + sunlight
Light reactions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],H + H + H + H + H + H + H + H + H + H + H + H + H + H + H + H + H + H + H + H + H + H +
The ATP that Jack built ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],sunlight breakdown of C 6 H 12 O 6 respiration photosynthesis ATP H + ADP + P i H + H + H + H + H + H + H + H +
ETC of Respiration ,[object Object],[object Object],generate H 2 O
ETC of Photosynthesis ,[object Object],[object Object]
Pigments of photosynthesis  ,[object Object],[object Object],[object Object],[object Object],Why does this molecular structure make sense?
A Look at Light ,[object Object],R O Y G B I V
Light: absorption spectra ,[object Object],[object Object],[object Object],[object Object],Why are plants green?
Photosystems of photosynthesis  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],reaction center antenna pigments
ETC of Photosynthesis Photosystem II Photosystem I
ETC of Photosynthesis 1 3 4 ATP H + H + H + ADP + P i H + H + H + H + H + H + H + H + to the Calvin Cycle
1 2 3 4 ATP ETC of Photosynthesis H + H + H + ADP + P i H + H + H + H + H + H + H + H + to the Calvin Cycle
ETC of Photosynthesis 6 5 $$ in the bank… reducing power electron carrier to the Calvin Cycle
ETC of Photosynthesis split H 2 O
ETC of Photosynthesis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Experimental evidence ,[object Object],[object Object],Proved O 2  came from H 2 O  not  CO 2  = plants split H 2 O 6CO 2 6H 2 O C 6 H 12 O 6 6O 2 light energy  + + + 6CO 2 6H 2 O C 6 H 12 O 6 6 O 2 light energy  + + + Experiment 1 6C O 2 6H 2 O C 6 H 12 O 6 6O 2 light energy  + + + Experiment 2
Noncyclic Photophosphorylation ,[object Object],[object Object],[object Object]
Cyclic photophosphorylation ,[object Object],[object Object],[object Object],X
Photophosphorylation  noncyclic photophosphorylation cyclic photophosphorylation
Photosynthesis summary  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],… stay tuned for the Calvin cycle
Any Questions??
Stomates

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Chapter 16 lecture 1

  • 2. Photosynthesis: Life from Light, Air & Water
  • 3.
  • 4.
  • 5. How are they connected? Heterotrophs Autotrophs making energy & organic molecules from light energy making energy & organic molecules from ingesting organic molecules Where ’ s the ATP? exergonic endergonic glucose + oxygen  carbon + water + energy dioxide C 6 H 12 O 6 6O 2 6CO 2 6H 2 O ATP  + + + + water + energy  glucose + oxygen carbon dioxide 6CO 2 6H 2 O C 6 H 12 O 6 6O 2 light energy  + + +
  • 6. Energy cycle Photosynthesis Cellular Respiration O 2 CO 2 plants animals , plants ATP The Great Circle of Life,AICE Kiddies H 2 O sun glucose
  • 7.
  • 8.
  • 10. Chloroplasts Chloroplasts Leaf Leaf Chloroplast absorb sunlight & CO 2 make energy & sugar Chloroplasts contain Chlorophyll CO 2
  • 11.
  • 12.
  • 13.
  • 14.
  • 15.
  • 16.
  • 17.
  • 18.
  • 19.
  • 20.
  • 21.
  • 22.
  • 23.
  • 24. ETC of Photosynthesis Photosystem II Photosystem I
  • 25. ETC of Photosynthesis 1 3 4 ATP H + H + H + ADP + P i H + H + H + H + H + H + H + H + to the Calvin Cycle
  • 26. 1 2 3 4 ATP ETC of Photosynthesis H + H + H + ADP + P i H + H + H + H + H + H + H + H + to the Calvin Cycle
  • 27. ETC of Photosynthesis 6 5 $$ in the bank… reducing power electron carrier to the Calvin Cycle
  • 28. ETC of Photosynthesis split H 2 O
  • 29.
  • 30.
  • 31.
  • 32.
  • 33. Photophosphorylation noncyclic photophosphorylation cyclic photophosphorylation
  • 34.

Editor's Notes

  1. So, in effect, photosynthesis is respiration run backwards powered by light. Cellular Respiration oxidize C 6 H 12 O 6  CO 2 & produce H 2 O fall of electrons downhill to O 2 exergonic Photosynthesis reduce CO 2  C 6 H 12 O 6 & produce O 2 boost electrons uphill by splitting H 2 O endergonic
  2. A typical mesophyll cell has 30-40 chloroplasts, each about 2-4 microns by 4-7 microns long. Each chloroplast has two membranes around a central aqueous space, the stroma. In the stroma are membranous sacs, the thylakoids. These have an internal aqueous space, the thylakoid lumen or thylakoid space. Thylakoids may be stacked into columns called grana.
  3. Not accidental that these 2 systems are similar, because both derived from the same primitive ancestor.
  4. NADH is an input ATP is an output O 2 combines with H + ’s to form water Electron is being handed off from one electron carrier to another -- proteins, cytochrome proteins & quinone lipids So what if it runs in reverse??
  5. Two places where light comes in. Remember photosynthesis is endergonic -- the electron transport chain is driven by light energy. Need to look at that in more detail on next slide
  6. Photons are absorbed by clusters of pigment molecules ( antenna molecules) in the thylakoid membrane. When any antenna molecule absorbs a photon, it is transmitted from molecule to molecule until it reaches a particular chlorophyll a molecule = the reaction center . At the reaction center is a primary electron acceptor which removes an excited electron from the reaction center chlorophyll a. This starts the light reactions. Don’t compete with each other, work synergistically using different wavelengths.
  7. Two places where light comes in. Remember photosynthesis is endergonic -- the electron transport chain is driven by light energy. Need to look at that in more detail on next slide
  8. PS II absorbs light Excited electron passes from chlorophyll to the primary electron acceptor Need to replace electron in chlorophyll An enzyme extracts electrons from H 2 O & supplies them to the chlorophyll This reaction splits H 2 O into 2 H + & O - which combines with another O - to form O 2 O 2 released to atmosphere Chlorophyll absorbs light energy (photon) and this moves an electron to a higher energy state Electron is handed off down chain from electron acceptor to electron acceptor In process has collected H + ions from H 2 O & also pumped by Plastoquinone within thylakoid sac. Flow back through ATP synthase to generate ATP.
  9. Need a 2nd photon -- shot of light energy to excite electron back up to high energy state. 2nd ETC drives reduction of NADP to NADPH. Light comes in at 2 points. Produce ATP & NADPH
  10. Two places where light comes in. Remember photosynthesis is endergonic -- the electron transport chain is driven by light energy. Need to look at that in more detail on next slide
  11. 1 photosystem is not enough. Have to lift electron in 2 stages to a higher energy level. Does work as it falls. First, produce ATP -- but producing ATP is not enough. Second, need to produce organic molecules for other uses & also need to produce a stable storage molecule for a rainy day (sugars). This is done in Calvin Cycle!