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TLC analysis system
PVA-Alg system PVA-Alg immobilization 48h TLC analysis 48h 0 h Biodiesel fuel from Jatropha oil via non-catalytic supercritical methanol transesterification
Comparison Biocatalysis Base Catalytic
Comparison 2 Base catalyst Biocatalyst
Different reaction time for the conversion rate FAME 24h 48h
Peanutand Soybean oil  Soybean Peanut Diluted sulfuric acid carbonization Iodine vapor
Conclusion 1.預期Serraia M 產的Lipase有高度選擇性,從上圖可以看岀有中間產物無法催化成FAME(fatty acid methyl ester),坦若使用Pseudomous進行催化則預測無此產生 2.鹼催化不殘留中間產物,反應完全高 3.TLC無法分辨大豆油和花生油差別 4.TLC可快速用於分析生質柴油是否產生
Alginate 3% immobilization System 3%                  5%                        7%                      10%                    13%                    15% (Oil: Catalyst  weight %)
Alginate immobilization system 看不同固定化重對大豆油轉化率影響 (算法= 菌種:大豆油重) 條件=甲醇:油 mol=1.3:1 溫度30℃             150RPM 時間1~2days
24h reaction 3%  5%  7%  10%  13%  15%
2 repeat Serratia lipase  features
Alginate system 24H  FAME
48h figure 今天下午四點驗收
FAME定量 HPLCand GC 1.內標法:和樣品一起打入分析(標準品濃度已知) 2.外標法:用已知的FAME作標準曲線
內標法internal  standard METHYL HEPTADECANOATE STANDARD FOR GC 十七烷酸甲酯1G  =3850NTD
外標法 methyl palmitate34.80USD methyl arachidate 78.30USD methyl stearate35.90USD methyl linolenate(100MG)  40.60USD methyl linoleate37.30USD methyl oleate35.90USD Total =262.8USD= 8409.6NTD
外標法(用於棕梠油) Methyl oleate35.90USD   methyl palmitate34.80USD   methyl stearate35.90USD   methyl linoleate37.30USD   methyl myristate45.00USD           total least: 188.9~~~6076.8NTD
Soybean oil
Project TLC系統(V) HPLC第二次分析(X) NEXT: 要使用哪個系統定FAME(biodiesel)量 比較固定化系統轉酯率 使用Pseudomous作轉酯化

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