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Stem cell therpay a hope to no hope disoders

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Stem cell therpay a hope to no hope disoders

  1. 1. Dr. Sharda Jain Dr. Ajay saxena Dr. Rajesh Dhall :A Hope to No Hope Disorders Stem Cell Therapy
  2. 2. Life is too Precious - don’t give up !! Mother Teresa
  3. 3. STEM CELL Therapy A HOPE TO NO HOPE DISORDERS
  4. 4. The fascinating new world of STEM CELL THERAPY : a medical revolution at the corner Welcome to
  5. 5. Future of Stem Cell Medicine • The Indian market potential slated to be in the range of US$ 500 million. • More than 45,000 people across the world receiving stem cell transplant every year. • Potential to reduce pharmaceutical R&D by 25% each year, with savings of up to US $25 million in each drug market. • Potential to be the next revolution in the field of medicine. LifeCare – ReeCure Centre of STEM CELL Therapy
  6. 6. “The Need” • The physical misery • Psychological trauma Of “No Hope Disorder” is great!! • No cure available for most of these diseases
  7. 7. Rationale of is stem cell Therapy ?? Introduction of adult stem cells into damaged tissue in order to treat injuries / Disease in “No Hope Disorders”
  8. 8. Stem cell potential … beating cardiac muscle
  9. 9. • Is it really working? • Why not studies are coming from premier institutes of country ? • Is it really possible to segregate Mesenchymal cells? • Differentiation of cells out side body possible in India??? • If it is too good then, why not NOBEL PRIZE given ? • ? • ?? • ??? • Is this therapy available in India? Questions ????
  10. 10. Times of India 9th October 2012
  11. 11. We are sure there are a lot of doubts plaguing your mind about STEM CELL BANKING and STEM CELL THERAPY. Let us help you to ERASE these doubts. Doubts… on SCT
  12. 12. 1913 the great Spanish neuroscientist Santiago Ramón y Cajal pronounced “that in adult Neurone & the nerve paths – Everything may die, nothing may be regenerated”. Nerve Cell Cannot Regenerate ?
  13. 13. Heart Brain Same is our perception for any tissue !!
  14. 14. Beliefs turned out to be FALSE as several types of cells can regenerate
  15. 15. Major Milestones Which Triggered Adult Stem Cell Research ? • Since1970’s, BONE MARROW TRANSPLANTS have been used for treatment of Immunodeficient & leukemia (with Good results).
  16. 16. 2nd Breakthrough… History of Human Embryonic Stem Cell Research In 1998, James Thomson (University of Wisconsin-Madison) isolated cells from the inner cell mass of the blastocyst, and developed the first human embryonic stem cell line in culture.
  17. 17. Prediction was made of Making cells and replacing all the diseased cells? & even gametes
  18. 18. Stem Cell Potential
  19. 19. Stem Cell History • 1970 – BM transplant for leukaemia • 1998 - Researchers first extract stem cells from human embryos • 1999 - First Successful human transplant of insulin-making cells from cadavers • 2001 - President Bush restricts federal funding for embryonic stem-cell research • 2002 - Juvenile Diabetes Research Foundation International creates $20 million fund-raising effort to support stem- cell research • 2003?? - California ok stem cell research • 2004 - Harvard researchers grow stem cells from embryos using private funding • 2004 - Ballot measure for $3 Billion bond for stem cells • 2006-7 – Private Banking for cord & cord blood in india. • 2014 – Public cord banking Kolkatta – Inst. Tropical med.
  20. 20. What are Stem Cells • Ability to differentiate • Ability to regenerate
  21. 21. Stem Cell is a •young, •primitive and unspecialized cell with a remarkable potential to renew, •Differentiate and Develop into any desired tissue or organ of the body. What is stem cells ?
  22. 22. Self renewal: Unlimited proliferative potential. Differentiation: Differentiate into various cell types Pluripotency, Multipotency, Unipotency) Plasticity : Trans-differentiation Regeneration potential: a means of Repair Characteristic Features
  23. 23. A single precursor What is Differentiation?
  24. 24. Cells having infinite self renewing capacity and potential for differentiation. What are Stem Cells?
  25. 25. 1000 IEC ICAs in biocompatible capsule IP Tx STZ Diabetic mice Blood glucose analysis of STZ induced diabetic mice transplanted with hUCMSCs and Islet like clusters (ILC) 0 10 20 30 0 100 200 300 400 500 hUCMSCs ILCs Normal Diabetic control Days after transplantationBloodglucoseconcentration mg/dl 0 25 50 75 100 125 150 0 100 200 300 400 ILC diabetic control non diabetic control time (mins) Bloodglucoseconcentration mg/dl Glucose Tolerance Test Fasting blood glucose concentration of diabetic mice after transplantation of ILCs showed that the hyperglycemic mice reverted back to normoglycemia. Intraperitoneal glucose tolerance test was performed on 45th day, post-transplantation. Mice transplanted with ILCs showed similar blood glucose profile as compared to the normal non diabetic controls.
  26. 26. How MSCs work In vivo Under normal circumstances MSC contribute to tissue maintenance, contributing to immune homeostasis by avoiding unnecessary immune cell response. With damaged tissue, harmonious interaction between MSC and endothelial cells are disrupted, allowing blood extravasations , blood clot form, platelet release multitude of bioactive molecule, immune cells are attracted where they start immune surveillance and immune response. MSC secrete bioactive molecules that exert anti-apoptotic, immunomodulatory , angiogenic , anti-scaring , anti-fibrotic and chemoattractant effects
  27. 27. Source of Adult Stem cells • Autologus, • Allogeneic or • Xenogenic Fetal tissue is the best current tissue source for human stem cells, However ethical issues are a major concern. (ICMR – Autologous)
  28. 28. The embryos of aborted fetuses Umbilical cord and cord blood of a new born baby Bone Marrow Peripheral Bloodstream Adult Tissues Amniotic Fluid MENSTRUAL BLOOD Dental Pulp Sources of Stem Cells Hair
  29. 29. Best Source Amniotic fluid, umbilical cord, Cord blood - Rich Source of Stem Cells
  30. 30. Cord Blood Stem Cells • Can be used only to cure chronic blood-related disorders such as sickle cell disease, Thalasemia, and leukaemia.
  31. 31. Umbilical Cord Blood Stem Cell Transplant • Umbilical cord blood stem cell transplants are less prone to rejection than either bone marrow or peripheral blood stem cells. This is probably because the cells have not yet developed the features that can be recognized and attacked by the recipient's immune system
  32. 32. Placental Blood as a Source of Hematopoietic Stem Cells for Transplantation into UNRELATED RECIPIENTS Joanne Kurtzberg, M.D.et al
  33. 33. PLASTICITY: The ability of one tissue to produce cells of other developmentally unrelated tissues Traditionally, stem cells were believed to be Lineage Restricted and Organ Specific. Bone marrow stem cells: The gold standard
  34. 34. AUTOLOGUS – STEM CELLS • While most blood stem cells reside in the bone marrow, a small number are present in the bloodstream. These multipotent peripheral blood stem cells, or PBSCs, can be used just like bone marrow stem cells to treat leukaemia, other cancers and various blood disorders
  35. 35. Adult Stem Cells - Hair
  36. 36. Dental Pulp Menstrual Blood
  37. 37. Allogeneic – Stem Cells • Sources of stem cells from another donor (allogeneic) are primarily relatives (familial- allogeneic) or completely unrelated donors (unrelated- allogeneic). The stem cells in this situation are extracted from either the donor's body or cord blood
  38. 38. Xenogeny - Stem Cells • In this stem cells from different species are transplanted, e.g. porcine fetal ventral mesencephalic (FVM) xenotransplants for Parkinson's disease. This has no major ethical concerns and a large amount of tissue is available, however life long immunosupression and risk of rejection are the major limitations
  39. 39. Prediction for Future Cells from these Animal will be used in human beings Without risk of rejection
  40. 40. THE SUCCESS OF STEM CELL THERAPY DEPENDS ON LAB.
  41. 41. These are • Mono Nuclear cells ( MNC ) • Hematopoietic Stem Cells [HSC] family: CD34+; CD45; CD133 • Mesenchymel count in M.N.C is just 0.001% • Mesenchymel stem cell [MSC]: CD73; CD90; CD106 Etc .......... Classification Of Stem Cells
  42. 42. Differentiation with different cluster coding/desire cells type ( as per disorder) through culture.. (Mesenchymal types are - CD 73, CD 90, CD 105 etc . .. . . • Multiplication up label of required cells quantity.( near to 1.5 Cr.). • Composition of all cells like Mnc, Hsc & Msc. Technology Behind Success
  43. 43. Bone Marrow Menstrual BloodHair Follicle Adipose Tissue STEM CELL THERAPY
  44. 44. Homogeneous population and high rate of cell division Homogeneous population and high rate of cell division No risk of rejection – USED across HLA barrier No risk of rejection – USED across HLA barrier No ethical issues No ethical issues Immune privileged Immune privileged Greater potency of cultured expanded product Greater potency of cultured expanded product Precise identification Precise identification Ease of isolation and scale up Ease of isolation and scale up Genetic stability Genetic stability Advantages ofAdvantages of MesenchymalMesenchymal Stem CellsStem Cells No risk of teratoma formation No risk of teratoma formation PluripotentPluripotent Efficient large scale expansion Efficient large scale expansion Lower cost of cell culture process Lower cost of cell culture process Why MSCs ?
  45. 45. Indications
  46. 46. Indications STEM CELL Therapy
  47. 47. • Diabetes • Cerebral Palsy • Multiple Sclerosis • Parkinson’s Disease •Alzheimer’s Disease • Autism • Anti-Ageing Very Effective
  48. 48. Diabetes STEM CELL THERAPY FOR DIABETES
  49. 49. Neuro-Muscular Disorders • Cerebral Palsy √ • Multiple Sclerosis √ • Parkinson’s Disease √ • Autism √ • Spinal Cord Injury • Stroke •Alzheimer’s Disease • Muscular Dystrophy STEM CELL Therapy for Neuro – Muscular Disorders
  50. 50. STEM CELL THERAPY FOR Anti-Ageing
  51. 51. Effective • Liver Cirrhosis • Non-healing fractures • Stroke • Osteogenesis Imperfecta • Muscular Dystrophy • Myocardial Infarction • Spinal Muscular Atrophy • Cardiomyopathy • ALS • Chronic Heart Failure • Osteoarthritis • Osteogenesis Imperfecta • Non-healing fractures
  52. 52. • Osteoarthritis • Non-healing fractures • Osteogenesis Imperfecta • Achondrodysplasia Bone and Cartilage Disorders Cardiovascular Disorders • Chronic Heart Failure • Myocardial Infarction • Cardiomyopathy • Buerger’s Disease STEM CELL Therapy for Bone and Cartilage Disorders
  53. 53. KIDNEY LIVER STEM CELL THERAPY FOR KIDNEY /LIVER DISORDER
  54. 54. Least Effective • Spinal Cord Injury • Buerger’s Disease • Eye Disorder
  55. 55. Not Effective/ Need More Trails Acute Leukaemia • Inherited Platelet Abnormalities • Chronic Leukaemia • Other Inherited Disorder • Myelodysplastic Syndromes • Inherited Metabolic Disorders • Marrow Failure • Histiocytic Disorders • Thalassemia • Inherited Erythrocyte Abnormalities • Myeloproliferative Disorders • Inherited Immune System Disorders • Lymphoproliferative Disorders • Plasma Cell Disorders • Phagocyte Disorders • Other Malignancies
  56. 56. Drug Testing - Quicker • Stem cells could allow scientists to test new drugs using human cell line which could speed up new drug development. • Only drugs that were safe and had beneficial effects in cell line testing would graduate to whole animal or human testing. • It would allow quicker and safer development of new drugs.
  57. 57. Let’s Create a better tomorrow for Spread the message Please create Public Awareness Spinal injury My cord blood treated Bhai’s thalassemia major Cured of insulin dep. DM Super man Ajit Jogi
  58. 58. Only company in India with 9 type of stem cell banking and treatment through Reecure 50 centres spread across India Mr. Ajay Saxena Ph. 8467888005 Website : www.reelabs.com Email : ajay.saxena@reelabs.com Q.& A.session Dr. Sharda Jain Mr. Ajay Saxena
  59. 59. STEM CELL Therapy A HOPE TO NO HOPE DISORDERS Thank you

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