Please answer everything andd show all steps please and thank you. I just don\'t have enough time with 4 midterms Biology 2007 Mini-quiz 4 1. A group of Biology 207 students was interested in determining if two compounds found in sunscreen (PABA and oxybenzone) are mutagenic. They tested this by exposing yeast cells that were auxotrophic for methionine to increasing concentrations of PABA, oxybenzone, EMS and toothpaste. The students used toothpaste as a control because they assumed it was neither mutagenic nor toxic. They used EMS because it is known to be mutagenic. They plated 0.1ml from a 10 dilution of the original culture onto YEPD plates. They plated 0.1ml of a 10- 1 dilution onto YM plates. The results from their experiment are shown below 450 400 350 o 300 250 200 150 100 50 100 60 80 120 Concentration (mM) toothpaste PABA oxybenzone --EMs Figure 1. The growth response of metA S.cerevisiae cells in response to exposure to different concentrations of toothpaste, PABA, oxybenzone and EMS on YEPD media Solution A: Ans: The concentration of the original culture can be calaculated by the formula CFU/mL (Concentration) = (No. of Colonies * dilution factor) / Volume of culture plated. From the first graph we can see that the Yeast cells have grown well in YEPD medium. No of colonies = 400. The diultion factor = 10 7 Volume of culture plated = 0.1 mL Therefore CFU/mL = 400*10 7 / 0.1 = 4 * 1010 CFU/mL B) Ans: The frequencey of sponatneous reversion = No of spontaneous reversions observed / Total no of cells in population. Sponatneous reversion was opbserved when cells are grown in YM medium. No of sponatenous reversion for EMS = (20+100+50) = 170 No of sponatneous reversion for Oxy benzene = (30+65+20) = 115 Mutant freuquency for EMS = 170/ 4 x1010 = 425 x 109 reversions / cell division Muatnt frequency fpr Oxy benzene = 115 / 4 x 1010 = 287 x 109 reversions / cell division c) Ans: The proportion of survivals of PABA and EMS in YEPD medium is From first graph PABA: EMS = 350:175 = 2:1 The proportion of survival of PABA and EMS in YM medium is From second graph PABA :EMS = 5:100 = 1:20.