Escherichia coli (e.c oli) : gram-ve bacteria

1. Escherichia Coli (E. Coli):
Presented by:
Khushbu’s Microbiology

2. ESCHERICHIA
COLI:MORPHOLOGY
Shape – : Rod shape (bacillus) bacterium.
Size –About 1–3 µm × 0.4–0.7 µm (micrometer).
Arrangement Of Cells – singly or in pairs.
Motility – motile bacterium. Some strains of E. coli are non-motile.
Flagella – flagellated bacterium with peritrichous flagella arrangement.
Spores –non–sporing bacterium.
Capsule – Capsules are present in some strains of the E. coli which can
easily be demonstrated using India ink preparation, appear as a clear
halo in a dark background.
Gram Staining Reaction –Gram -ve (Negative) bacterium.

3. E.Coli :
gram –Ve Bacteria
 Escherichia coli, also known as E.
coli, is a Gram-negative, facultative
anaerobic, rod-shaped,
 coliform bacterium of the genus
 Escherichia that is commonly found
in the lower intestine of warm-
blooded organisms (endotherms).

4. CLASSIFICATION OF E.COLI
Domain: Bacteria
Kingdom: Bacteria
Phylum: Proteobacteria
Class: Gamma Proteobacteria
Order: Enterobacteriales
Family:
Enterobacteriaceae
Genus: Escherichia
Species: Escherichia coli (E. coli)

5. NUTRITION
 E. Coli : known to be as heterotrophic organisms. “An organism
that cannot synthesize its own food and is dependent on complex
organic substances for nutrition” .They use their host to take in
carbon from the biosynthesis process.
 The main source of E. Coli’s carbon supply comes from glucose
molecules that are previously ingested by the host.
 The glucose is then transformed into carbon by the process of
centre metabolism.
1. The conversion of glucose into pyruvate by the Embden-
Meyerhof Parnas Pathway.
2. The oxidization of acetyl into carbon dioxide by the Tricarboxylic
Acid.
The pyruvate that is formed can also be transformed
into ATP by fermentation.

6. E.Coli Cell Diagram

7. E.Coli on different Media
E.Coli on MacConkey’s
Agar
MacConkey’s Agar E.Coli on EMB AGAR

8. E.Coli

9.  THANKS