The document discusses different types of cell culture used in bioreactors. It describes organ culture, tissue culture, and cell culture. Cell culture involves dispersing tissue enzymatically into a cell suspension that can be grown as a monolayer or in suspension. Continuous cell lines can be propagated indefinitely and have gained immortality through transformation. Bioreactors must provide a well-controlled environment for cell culture and can operate in batch, fed-batch or perfusion modes. Common bioreactor designs include stirred tank, airlift and wave bioreactors.

1. TYPES OF CELL CULTURE IN BIOREACTORS

9. Current Choices of Host Cells in Biotech Bacteria Cells Yeast Transgenic Animals Transgenic Plants Animal Cells

10. Comparison of Monoclonal Antibody Produced from CHO & Transgenic Goats Assumption Annual Yield (Kg/yr) Batch Yield (grams/L) 60 goat herd 350 L/animal year 40 5.0 Transgenic Goats Grange Castle 6 X 12,500 L Bioreactors 4000 3.4 CHO Bioreactor

11. The Majority of Biotech Products on the Market Are Made in Animal Cells

12. Comparison of Animal and Microbial Culture 10 6 cells/mL 10 9 -10 10 cells/mL Growth density 10 5 cells/mL 1 cell Seeding density 10000-100000 nm 100-2000 nm Size Very susceptible Less affected Environmental FX Key for buffering Sometimes CO 2 Requirement Complex Usually simple Nutritional Rqmt Low High O 2 Requirement 1-5% per hour 10-50% per hour Growth Rate Present Present Cell membrane Generally absent Generally present Cell wall Animal Cells Microbes Features

20. Development of Cell Lines

34. Batch Curve

44. Perfusion Culture

49. McLimans' group developed the first "spinner flasks" in 1957. Present Model Original Model

53. Bioreactor Design Airlift Reactors Stirred Tank Reactor

54. Stirred Tank Bioreactor

58. Wave Bioreactor

59. Wave Bioreactor

60. Wave-induced Agitation

63. Wave Bioreactor in Perfusion Mode

64. Packed-bed and fluidized-bed biofilm or immobilized-cell bioreactor

65. Tissue culture flasks (T-flasks)

66. Hollow Fiber Bioreactor