DIAGNOSIS OF TUBERCULOSIS(CULTURAL)

1. Presented by :ARIF KHAN
4TH GROUP
5TH YEAR

2.  Tuberculosis is a diversified disease.
 Any organs can be involved.
 Any age group, gender no bar for
Tuberculosis.
 Involvement of Lungs contribute to
majority of tuberculosis.
 And involvement of Lungs is designated as
Pulmonary tuberculosis.
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3.  Majority of Adults suffer with pulmonary
tuberculosis.
 Microbiological examination of Sputum
continues to be a Gold standard in proving
the Diagnosis.
 Sputum examination in Children is not
sensitive in Diagnosis.
 Radiological examination of Lungs, most
commonly prescribed investigation.
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4. 4

5. Culturing for isolation of
Mycobacterium spp continues
to be a Gold standard,
particularly in Developing
countries.
Need only 10 – 100 bacilli / 1
ml of sputum.
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6.  Surveillance,
 Drug sensitivity testing patterns.
 Identify treatment failures.
 Useful in Patients presenting with
respiratory symptoms, X- ray’s
suggestive, but smear negative. Can prove
culture positive.
 Cultures remain suggestive and helpful in
early treatment periods, failed drug
regimes.
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7.  Culturing on
Lowenstein
Jenson’s culture
medium remain
the affordable
,economical
method in
developing world.
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8.  Mycobacterium spp are slow growing.
 Need 6 – 8 weeks for growing.
 Specimens can be contaminated while
growing, needs repeated specimens, in turn
patients loose confidence in Laboratories.
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9.  Useful in HIV infected patients with
Tuberculosis.
 As even few bacilli can be grown in spite of
smear negativity.
 But the specimens to be incubated for longer
time as few bacilli are present.
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10.  Specificity is lost due to contamination.
 Can yield false positive results in 1 – 4 % of
the cases.
 Cultures may be negative in spite of x rays
are suggestive of tuberculosis.
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