1. REDUCTION OF PERCHLORATE BY MAGNETOPIRULLUM sp. VITRJS5
Harshini.E, Aamani.D, J.Esther Jo Vita, Suthindharan.K
School of Biosciences and Technology
VIT UNIVERSITY,VELLORE,TAMIL NADU.
MATERIALS AND METHODS
Culturing of Microorganism
Three sets of experiment with acetate as electron donor
Oxygen Nitrate Perchlorate
(electron acceptor) (electron acceptor) (electron acceptor)
Statistical analysis
ABSTRACT
Perchlorate is a toxic by-product, which contaminate the
soil and water bodies. Perchlorate reducing bacteria has
the ability to utilize electron donor (acetate) and reduce
perchlorate (electron acceptor). Magnetospirullum sp.
VITRJS5, is a novel perchlorate reducing bacterium, and
found to have perchlorate reducing activity. In this work
various statistical analysis have been done to indicate the
growth of Magnetospirullum sp. VITRJS5 in different
concentrations of perchlorate present in the media and
also their ability to reduce the perchlorate.
INTRODUCTION
•Perchlorate is the salts derived from the perchloric acid.
•The physical and chemical properties of perchlorate also
make it impractical to remove the anion by common
treatment approaches .
• A number of perchlorate reducing bacteria have been
isolated and studied and they are microaerophilic in
nature.
• Magnetotactic bacterium was isolated from the fresh
water sediment. They are phylogenetically closer to the
perchlorate degrading Dechlorospirullum species.
•They use electron donor like acetate to reduce electron
acceptors like nitrate, perchlorate etc.
CONCLUSION
The novel Magnetospirullum strain sp. VITRJS5 was able to
reduce oxygen, nitrate and perchlorate as electron acceptor by
utilizing electron donor(acetate).As Magnetospirullum strain
sp. VITRJS5 were cultured in same conditions and it started
gaining the ability to degrade perchlorate and nitrate. The mean
and standard deviation values for the first and the second set
was found to be : O1-0.192,0.138, N1-0.111,0.091, P1-0.099,
0.1,and O2 -0.251,0.148 , N2-0.216,0.091 ,-P2 -0.1651,0.283
respectively. Thus Magnetospirullum VITRJS5 can be further
used for degradation kinetic studies of Perchlorate with nitrate
and oxygen as competitive electron acceptors.
REFERENCE
•Cameron Thrash J, Magnetospirullum bellicus sp. nov., a Novel Dissimilatory
Perchlorate-Reducing Alphaproteobacterium Isolated from a Bioelectrical Reactor, Appl
Environ Microbiol. Jul 2010; 76(14): 4730–4737.
•Coates, J. D., and L. A. Achenbach. 2004. Microbial perchlorate reduction: rocket-
fuelled metabolism. Nature Review Microbiology. 2:569–580.
•Chaudhuri, S. K. et al., 2002. Environmental factors that control microbial
perchlorate reduction, Applied Environmental Microbiology 68:4425–4430.
ACKNOWLEDGEMENT
We sincerely thank Dr. Suthindharan and Marine
biotechnology and Biomedicine Lab , VIT university for
helping us to complete this project as a successful one.
0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
0 1 2 3 4 5 6 7
ODat600nm
Hours
Growth cuvre
1.5mg
2.5mg
5mg
12.5mg
20mg
30mg
0
0.002
0.004
0.006
0.008
0.01
0.012
0.014
0.016
0 1 2 3 4 5 6 7
SpecificGrowthRate1/hr
Starting Acetate Concentration mg/L
oxy
0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0 1 2 3 4 5 6
ODat600nm
Time (Hours)
OD Vs Time
2.4grm 3.75grm
6.5grm 16grm
41grm 50grm
Scale-
Y-axis 1cm= 0.05 unit
X-axis 1cm= 24hrs
-0.05
0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0 2 4 6 8 10 12
ODat600nm
Hours
Growth Curve
1.6grm
2.4grm
3.75grm
6.5grm
16grm
41grm
0
0.01
0.02
0.03
0.04
0.05
0.06
0 1 2 3 4 5 6 7 8
SpecificGrowthRate1/hr
Starting Acetate Concentration (mg/L)
0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
0 2 4 6 8
ODat600nm
Time (Hours)
OD Vs Time
1.25mg
2.5mg
5mg
12.5mg
20mg
30mg
Scale-
Y-axis 1cm= 0.05 unit
X-axis 1cm= 24hrs
0
0.01
0.02
0.03
0.04
0.05
0.06
0 1 2 3 4 5 6 7
SpecificGrowthRate1/hr
Starting Acetate Concentration (mg/L)
MEAN(Oxygen) STANDARD
DEVIATION
First
set
Second
set
First
set
Second set
0.085 0.0872 0.045 0.045
0.085 0.08917 0.045 0.046
0.09 0.123 0.058 0.062
0.1295 0.17767 0.090 0.07149
0.1318 0.196 0.08 0.108
0.1925 0.251 0.138 0.148
MEAN (Nitrate) STANDARD
DEVIATION
First
set
Second
set
First set Second set
0.0335 0.0728 0.043 0.043
0.0635 0.0812 0.038 0.038
0.0451 0.0668 0.034 0.0346
0.0685 0.1266 0.043 0.0435
0.1069 0.207 0.083 0.0831
0.1111 0.2164 0.091 0.0919
MEAN (Perchlorate) STANDARD DEVIATION
First set Second set First set Second set
0.016 0.098 0.025 0.084
0.009 0.08 0.009 0.099
0.009 0.09 0.007 0.059
0.0223 0.127 0.0.023 0.113
0.017 0.345 0.013 0.296
0.099 0.1651 0.100 0.283
0
0.05
0.1
0.15
0.2
0.25
0.3
0 1 2 3 4 5 6 7
ODat600nm
Time (Hours)
OD Vs Time
2.4mg
3.75mg
6.5mg
16mg
41mg
50mg
Scale-
Y-axis 1cm= 0.05 unit
X-axis 1cm= 24hrs
0
0.005
0.01
0.015
0.02
0.025
0.03
0.035
0 1 2 3 4 5 6 7
SpecificGrowthRate1/hr
Starting Acetate Concentration (mg/L)
0
0.01
0.02
0.03
0.04
0.05
0.06
0.07
0.08
0 1 2 3 4 5 6 7
SpecificGrowthRate1/hr
-0.1
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0 1 2 3 4 5 6 7
ODat600nm
Time (Hours)
OD Vs Time
2.4mg 3.75mg
6.5mg 16mg
41mg 50mg
Scale-
Y-axis 1cm= 0.05 unit
X-axis 1cm= 24hrs
0
0.02
0.04
0.06
0.08
0.1
0.12
0.14
0 1 2 3 4 5 6 7
SpecificGrowthRate1/hr
Starting Acetate Concentration (mg/L)
Set -2
Set 1
RESULTS AND DISCUSSION