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- 1. Ecological and evolutionary functional genomics: finding & studying genes that matter Christopher W. Wheat
- 3. Rapid transcriptome characterization for a nonmodel organism using 454 pyrosequencing C. Vera and C. Wheat, Fescemyer, Frilander, Crawford, Hanski, Marden 2008
- 4. 48k contigs
- 5. First de novo transcriptome assembly using 454 pyrosequencing SNP’s
- 6. Annotation of 45,000 contigs + singletons Predicted genes: D. melanogaster = 13,379 B. mori = 18,510 Estimated coverage : 70% D. mel estimate 50% B. mori estimate Lower estimate 50% genes
- 7. Upper estimate of 70% = 13,142 genes
- 13. Mixed Model Analysis : popage & peak metabolic rate Popage fixed effects: dye popage bodymass popage*bodymass PMR fixed effect: dye PMR Random effects: slide array(slide) spot spot*array individual (FDR threshold = 5%) Using JMP Genomics (SAS)
- 15. Significantly different genes (FDR threshold = 5%) Peak Metabolic Rate Continuous Wheat et al., in prep. 623 33 188 656 Population Age 221 Categorical
- 18. Temperature treatments Last instar larvae Reared in lab Study gene expression Kvist et al., in prep. 20 o C 26 o C 35 o C Temperature maximum Cold Standard Hot Treatments Hot Standard Cold