letter of recommondation from Gilles Travé, my boss during my work experience for experimental thesis in ESBS (ecole supériore de biotechnologie in Strasbourg.

1. Gilles TRAVE, PhD
Directeur de recherche au CNRS
Responsable de l'équipe Oncoprotéines
Institut de Recherche de l’Ecole de
Biotechnologie de Strasbourg
Boulevard Sébastien Brant
BP 10413
F-67412 Illkirch cedex
Tel +33 3 68 85 47 20
C. gilles.trave@unistra.fr
Laboratoire de
Biotechnologie et
Signalisation cellulaire
UMR 7242
Institut de Recherche de
l’École de Biotechnologie
de Strasbourg
Illkirch,
le
9
juillet
2015
To
whom
it
may
concern
:
I
hereby
confirm
that
Giorgia
Ricci,
a
master
student
(second
year)
from
the
University
of
Roma,
has
performed
a
research
stay
in
our
laboratory
in
the
frame
of
the
Erasmus
program.
She
has
been
in
our
laboratory
for
almost
10
months,
starting
from
september
8th,
2014
and
finishing
on
june
28th,
2015.
With
her
stay
in
our
lab,
she
obtained
30
european
credits
for
her
studies.
In
addition
to
the
research
stay,
she
followed
several
courses
in
the
University
of
Strasbourg.
In
our
laboratory,
Giorgia
has
started
a
new
research
project
under
the
close
supervision
of
my
collaborator
Anne
Forster.
With
Anne,
Giorgia
has
learnt
all
the
practical
aspects
of
construct
planning,
cloning,
sequencing,
sequence
analysis
and
validation,
recombinant
protein
expression
in
bacteria,
and
analysis
of
protein
expression
on
SDS
PAGE.
For
all
these
aspects
she
has
received
theorical
explanations,
have
been
allowed
to
follow
the
procedures
performed
by
others,
and
then
as
a
last
step
she
has
performed
all
procedures
herself,
thereby
acquiring
a
full
autonomy
for
these
steps.
She
has
been
introduced
to
two
alternative
methods
of
cloning
:
classical
ligation
in
open
vectors
as
well
as
cloning
by
homologous
recombination.
She
has
also
received
an
introduction
to
protein
purification
strategies,
and
has
followed
several
purification
procedures,
performed
either
by
Anne
or
myself.
She
has
also
been
introduced
to
the
GPCA
approach,
a
novel
method
for
protein-­‐protein
interaction
analysis
in
mammalian
cells.
Moreover,
and
most
importantly,
Giorgia
has
followed
and
fully
understood
the
entire
strategic
planning
of
her
research
project,
and
she
has
also
gained
quite
some
insight
in
the
bibliographic
and
scientific
background
justifying
the
project.
This
project
consisted
in
analyzing
the
sequence
of
a
850-­‐amino
acid
enzyme,
whose
more
than
50%
of
the
3D
structure
is
unknown,
predicting
the
potential
folded
domains,
of
the
protein,
and
planning
expression
constructs
which
would
correspond
to
folded
and
well-­‐delimitated
fragments
of
the
protein,
with
as
a
final
aim
the
production
and
purification
of
these
fragments
for
structural
studies.
All
these
steps
have
been
effectively
performed,
and
the
very
week
when
Giorgia
left
the
lab,
the
first
purified
samples
of
one
600-­‐
amino
acids
fragment
were
purified
and
subjected
to
the
first
crystallisation
screens.
Giorgia
has
been
a
very
pleasant,
thoutful
and
intelligent
person
to
have
in
the
laboratory.
All
the
team
has
very
much
appreciated
her
very
nice,
well-­‐balanced
character,
always
kind
and
careful
about
others
in
the
group.
She
is
a
very
motivated
student
with
a
very
good
theoretical
background,
always
eager
to
learn
and
to
understand
not
only
her
particular
project,
but
also
the
general
thematics
of
the
lab.
Besides,
she
also
showed
very
good
communication
skills,
by
learning
the
french
language
with
incredible
facility
and
rapidity.
In
conclusion,
we
all
have
very

2. much
enjoyed
working
with
Giorgia,
and
hope
her
all
the
best
–
which
she
highly
deserves-­‐
for
the
end
of
her
studies
as
well
as
her
subsequent
professional
carreer.
Sincerely,
Gilles
TRAVÉ