Not necessarily. It could very well mean that you have not used aneluent that is sufficiently polar to displace any cpds. I would trya more polar eluent to see if separation occurs. Hope this helps, Steve Solution Not necessarily. It could very well mean that you have not used aneluent that is sufficiently polar to displace any cpds. I would trya more polar eluent to see if separation occurs. Hope this helps, Steve.