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Plant Reproduction
ENOCH B. TACLAN
What is Plant Reproduction
 Reproduction is one of the most important characteristic of all
living beings. It is the production of ones own kind.
 It is necessary for the continuation of the species on earth and also to
replace the dead members of the species.
 The process by which living organisms produce their offspring's for the
continuity of the species is called reproduction.
The modes of reproduction vary according to individual species and
available conditions. It may be simply by division of the parent cell as in
unicellular organisms, by fragmentation of the parent body, by formation
of buds and spores, or it may be very elaborate involving development of
male and female reproductive organs (stamens and pistils). Irrespective of
the mode of reproduction, all organisms pass on their hereditary material
to their offspring during the process of reproduction (Reproduction and
Heredity, Biology Module Chapter 18)
Plant Reproduction
Asexual Reproduction
No Gametes
Only one parent needed
New organism genetically identical to
parent
Plants and bacteria reproduce this way
Asexual Reproduction
 Fission : As in unicellular organisms like bacteria where the content of the
parent cell divides into 2, 4 or 8 daughter cells and accordingly the fission
is known as binary or multiple fission. Each newly formed daughter cell
grows into a new organism.
 Budding : A bud like outgrowth is formed on one side of the parent cell
and soon it separates and grows into a new individual e.g. in yeast.
 Fragmentation : In filamentous algae, an accidental breaking of the
filament into many fragments, each fragment may give rise to a new
filament of the algae by cell division e.g. Spirogyra, green algae.
 Spore formation : In lower plants like bryophytes reproductive units
develop asexually on the parent body. These are called spores. They are
microscopic and covered by protective wall. When they reach the suitable
environment they develop into a new plant body e.g. in bread moulds,
moss, fern.
Plant Reproduction
Sexual Reproduction
Male and Female Gametes involved
Two parents are needed
New organism will not be genetically
identical to either of the parent
Reproduction in Lower Plants
 Chlamydomonas (A Unicellular Alga)
 Asexual-with the help of zoospores
 Chlamydomonas loses flagella and becomes non-motile.
 Its protoplasm (cytoplasm and nucleus) divides mitotically and
forms 4-8 zoospores.
 The parent cell wall is ruptured and zoospores are released.
 Each zoospore develops a cell wall and grows into an adult
cell.
 The parent cell does not exist, any more.
Reproduction in Lower Plants
 Sexual Reproduction
 The cell becomes non-motile by losing its flagella.
 The protoplasm divides mitotically into 2, 4, 8, 16, 32 daughter cells.
 Each daughter cell develops flagella and is released in water by the rupture of
mother cell wall. It acts as a gamete.
 The gamete is morphologically identical in structure but all identical
(Isogamous).
 Gametes released from two different mother cells fuse together.
 The contents of the gametes fuse and form a zygote (diploid). This is the only
diploid stage in the life cycle of Chlamydomonas.
 The zygote develops a thick wall around itself to tide over unfavourable
conditions (zygospores)
 On the return of favourable conditions (temperature, food and water) the
diploid nucleus divides by meiosis and forms four haploid zoospores.
 Each zoospore grows into a new adult Chlamydomonas
Vegetative Reproduction
 involves formation of new plantlets from
vegetative (somatic) cell, buds or organs of the
plant.
 It is similar to a sexual reproduciton in that it
also requires only mitotic division, no gametic
fusion and daughter plants are genetic clones
of the parent plant.
Spirogyra (A Multicellular Alga)
 Vegetative Reproduction by fragmentation
 The filament breaks into small fragments.
 Each fragment grows into a new filament by cell division
 Sexual Reproduction
 Two filaments come to lie very close to each other.
 Cells of the two filaments form a contact with the help of a tube called the
conjugation tube.
 Cytoplasmic contents of each cell round off to act as a gamete.
 Gamete from one cell (male) passes to the other cell (female) through the
conjugation tube.
 Each filament acts either as male or female.
 The contents of two gametes fuse in the female cell and form a diploid zygote
 The zygote develops a thick wall around itself and tides over the
unfavourable period.
 On the return of favourable conditions the diploid nucleus divides by
meiosis into four haploid nuclei. Three of these nuclei degenerate.
 On germination, wall of the zygote ruptures and a small tube like structure,
containing one haploid nucleus comes out.
 The small tube develops into 1 filament by repeated mitotic divisions.
Reproduction in Angiosperms
(Flowering plants)
 Angiosperms reproduce both vegetative and sexual methods
 Angiosperms reproduction occurs by fusion of male and female gametes
present in the flower.
 Angiosperms can be classified depending the time they take to complete
life cycle and flowering
 Annuals – Live for one year , produce flowers and seeds within one season
 Biennials - two seasons, first year vegetative state, second year produce
flowers, fruits and seeds
 Perennials – plants which live for several years , one to few years vegetative
stage then after that flowers, fruits and seeds every year
 Monocarpic - plants are those that flower, set seeds and then die
Plant Reproduction
The Flower:
The flower is the part of a plant
that is involved in sexual
reproduction
Plant Reproduction
 Initiation of flowering
 The young plant grows vigorously and continue to
grow till it attains a definite shape and size with its
vegetative parts (roots, stem, leaves) well developed
 Young or the Juvenile phase
 Reproductive phase or adult phase
 Vegetative shoot apex transforms into a reproductive
or floral apex and starts bearing flowers
Plant Reproduction
The Parts of the Flower
Sepals
Petals
Carpel
Stamen
Plant Reproduction
Sepal:
Green leaves which protect the
flower when it is a bud
Petals:
Attract insects to the flower
Plant Reproduction
 The Stamen:
 Male Part of the flower
 Filament – stalk part of stamen
 Anther – Small bag on top of the filament contains
pollen
Plant Reproduction
 Structure of the Carpel:
 Stigma – Pollen grains land here
 Style – Connects stigma to the ovary
 Ovary – Contains ovules
 Ovules – Contain female gametes – eggs
 Pistil - The ovule producing part of a flower
Plant Reproduction
Stages in Sexual Reproduction in Plants:
Pollination
Fertilisation and Seed Formation
Seed Dispersal
Germination
Plant Reproduction
Pollination: Happens when pollen
from one flower is transferred to
another flower of the same species
Insect Pollination(Entomophily) or
Wind Pollination (Anemophily)
Plant Reproduction
TWO TYPES OF POLLINATION
Self pollination – transfer of pollen grains to
stigma of same or another flower of same
plant
Cross pollination – transfer of pollen grains
to stigma of a flower of another plant of the
same species
Importance of Pollination
 It results in fertilization and ovule is converted into seed.
 New varieties of plants are formed through new combination
of genes in case of cross pollination.
 During pollination pollen tube produces growth hormones
which convert ovary into fruit.
 Cross pollination is brought about by various external
agencies such as, wind, insects, water, birds and other animals.
Plant Reproduction
 Insect Pollination:
 Petals are bright and perfumed.
 Insect rub against Anther and pollen gets stuck to
them
 Insect move to another plant of same species and
pollen sticks to sticky stigma
Plant Reproduction
 Wind Pollination:
 The flowers have long anthers which hang outside
the flower.
 Pollen is blown out of the anthers by the wind
 The stigma of a wind-pollinated flower is very large
and feathery – able to catch pollen
Plant Reproduction
 Fertilisation and Seed Formation:
 After pollination, pollen tube develops out of the pollen grain
and into the style,
 It then enters the ovule and male gamete travels down to join
with the egg – zygote
 The zygote then becomes the seed.
 The ovary will turn into the fruit
Plant Reproduction
Dispersal Of The Seed:
 The major function of the fruit is to scatter the seed
of the plant
 Animal Dispersal
 Wind Dispersal
 Water Dispersal
 Self Dispersal
Plant Reproduction
 Animal Dispersal:
 Edible Fruits: Animal eats fruit and eventually passes
the seed in its droppings, if seed falls in right place it
will grow into new plant
 Hooked Fruits: Fruits have tiny hooks on their surface,
sticks to the animals.
Plant Reproduction
 Wind Dispersal:
 The wind provides a very effective method of carrying
fruit away from the parent plant
 Dandelion uses parachute
 Sycamore tree uses winged fruits
Plant Reproduction
 Water Dispersal:
 The water lily is an example of a plant with
fruits that can float.
 Water currents carry the fruit away, with the
seeds inside
Plant Reproduction
 Self Dispersal:
 The gorse plant can scatter its own seed.
 In summer the plant dries out and suddenly bursts.
 The seeds shoot out from the fruit, and may land
some distance from the parent plant
Plant Reproduction
 Germination:
 Plumule – Will form shoot of plant
 Radicle – Will become the root of the plant
 Testa – Protects the seed
 Food Store – Provides nourishment for the
young plan
Plant Reproduction
 Conditions Necessary For Germination:
 Water
 Oxygen
 Warmth
Vegetative Reproduction in
Angiosperms
 Reproduction of new plants from the portion of the
vegetative parts of a plant is very common and is called
vegetative reproduction. Stems, roots, leaves and even buds
are variously modified to suit this requirement. This is called
natural vegetative reproduction.
 Natural Method : In natural methods, a portion of the plant
gets detached from the body of the mother plant and grows
into an independent plant. The parts may be stem, root, leaf
or even flower
 The underground modification of stem, like rhizome, (in ginger), tuber
(potato), bulb (onion) are provided with buds which develop into a new
plant and therefore used to carry out vegetative propagation of the plant
in the field. Plants with subaerial modification such as Pistia (offset) and
Chrysanthemum (sucker) are also used for vegetative propagation.
 Similarly, tuberous roots (Asparagus and sweet potato) can also be used
for propagation as these roots have adventitious (form accidentally) buds
which ground into a new plant.
 Sometimes even leaves contribute to propagation of plants leaves of
Bryophyllum that have buds on the margin and these buds grow into small
plantlets. When detached from the mother plant they grow into
independent plants.
 In plants like Agave and Oxalis multicellular bodies called bulbils develop
near the flower. These are called bulbils which when fall on the ground
grow into new plant
Vegetative Reproduction in
Angiosperms
 Humans have taken advantage of this natural phenomenon and have
artificially propagated plants vegetatively by using the specialized parts as
by cutting, grafting and layering.
 Cuttings: Many plants like rose, Bougainvillea, Croton, Coleus, money
plants, sugarcane etc. are grown through their stem cuttings. Cuttings of
these plants can be grown even in water where they strike roots and
develop adventitious buds
 Layering: In this method, a lower branch of a plant is bent down and
covered with moist soil leaving the growing tip above the soil. A ring of
bark is removed from the stem before it is bent down. In a few weeks time
when enough roots have developed on the underground portion above
the ringed part, it. is cut off from the parent plant and grown separately as
an independent plant.
Vegetative Reproduction in
Angiosperms
 Aerial layering or Goatee : In this method a ring of bark is removed from a
selected branch, and it is covered with moist moss and enclosed in a
polythene sheet when roots appear, the stem is cut below the roots and
planted to form a new plant
Vegetative Reproduction in
Angiosperms
 Grafting – The purpose of grafting is to combine one plant's qualities of
flowering or fruiting with the roots of another that offers vigour and
resilience. This is a difficult task and requires lots of skill and practice. In
most cases, trees and shrubs are available to buy already grafted onto a
rootstock.
 In this stock a branch is inserted which is known as scion or graft. This
scion or graft is the stem cutting from the desired plant. Usually the
grafted end of stock and scion fit well with each other and are bound
firmly with tape or rubber-band until their tissues unite and vascular
continuity is established. Grafting is mostly. practised in dicot plants
Advantages and Disadvantages of
Vegetative Reproduction
 Advantages
 (a) Rapid means of reproduction and spread.
 (b) Offsprings identical to parent. The desired varieties can
thus be preserved genetically for use.
 (c) Food storage organs allow perennation or survival in
adverse condition
 (d) Improved varieties of ornamental plants and fruit trees can
be multiplied easily.
 (e) Vegetative propagation is a quicker, easier and a less
expensive method of multiplying plants
 Disadvantages
 (a) Overcrowding and competition for space unless
separated artificially.
 (b) New varieties cannot be produced by this method
except by mutation.
 (c) Diseases typical of the species are rapidly
transmitted and can decimate a crop
Thank you

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Plant reproduction

  • 2. What is Plant Reproduction  Reproduction is one of the most important characteristic of all living beings. It is the production of ones own kind.  It is necessary for the continuation of the species on earth and also to replace the dead members of the species.  The process by which living organisms produce their offspring's for the continuity of the species is called reproduction. The modes of reproduction vary according to individual species and available conditions. It may be simply by division of the parent cell as in unicellular organisms, by fragmentation of the parent body, by formation of buds and spores, or it may be very elaborate involving development of male and female reproductive organs (stamens and pistils). Irrespective of the mode of reproduction, all organisms pass on their hereditary material to their offspring during the process of reproduction (Reproduction and Heredity, Biology Module Chapter 18)
  • 3. Plant Reproduction Asexual Reproduction No Gametes Only one parent needed New organism genetically identical to parent Plants and bacteria reproduce this way
  • 4. Asexual Reproduction  Fission : As in unicellular organisms like bacteria where the content of the parent cell divides into 2, 4 or 8 daughter cells and accordingly the fission is known as binary or multiple fission. Each newly formed daughter cell grows into a new organism.  Budding : A bud like outgrowth is formed on one side of the parent cell and soon it separates and grows into a new individual e.g. in yeast.  Fragmentation : In filamentous algae, an accidental breaking of the filament into many fragments, each fragment may give rise to a new filament of the algae by cell division e.g. Spirogyra, green algae.  Spore formation : In lower plants like bryophytes reproductive units develop asexually on the parent body. These are called spores. They are microscopic and covered by protective wall. When they reach the suitable environment they develop into a new plant body e.g. in bread moulds, moss, fern.
  • 5. Plant Reproduction Sexual Reproduction Male and Female Gametes involved Two parents are needed New organism will not be genetically identical to either of the parent
  • 6. Reproduction in Lower Plants  Chlamydomonas (A Unicellular Alga)  Asexual-with the help of zoospores  Chlamydomonas loses flagella and becomes non-motile.  Its protoplasm (cytoplasm and nucleus) divides mitotically and forms 4-8 zoospores.  The parent cell wall is ruptured and zoospores are released.  Each zoospore develops a cell wall and grows into an adult cell.  The parent cell does not exist, any more.
  • 7. Reproduction in Lower Plants  Sexual Reproduction  The cell becomes non-motile by losing its flagella.  The protoplasm divides mitotically into 2, 4, 8, 16, 32 daughter cells.  Each daughter cell develops flagella and is released in water by the rupture of mother cell wall. It acts as a gamete.  The gamete is morphologically identical in structure but all identical (Isogamous).  Gametes released from two different mother cells fuse together.  The contents of the gametes fuse and form a zygote (diploid). This is the only diploid stage in the life cycle of Chlamydomonas.  The zygote develops a thick wall around itself to tide over unfavourable conditions (zygospores)  On the return of favourable conditions (temperature, food and water) the diploid nucleus divides by meiosis and forms four haploid zoospores.  Each zoospore grows into a new adult Chlamydomonas
  • 8.
  • 9. Vegetative Reproduction  involves formation of new plantlets from vegetative (somatic) cell, buds or organs of the plant.  It is similar to a sexual reproduciton in that it also requires only mitotic division, no gametic fusion and daughter plants are genetic clones of the parent plant.
  • 10. Spirogyra (A Multicellular Alga)  Vegetative Reproduction by fragmentation  The filament breaks into small fragments.  Each fragment grows into a new filament by cell division  Sexual Reproduction  Two filaments come to lie very close to each other.  Cells of the two filaments form a contact with the help of a tube called the conjugation tube.  Cytoplasmic contents of each cell round off to act as a gamete.  Gamete from one cell (male) passes to the other cell (female) through the conjugation tube.  Each filament acts either as male or female.  The contents of two gametes fuse in the female cell and form a diploid zygote
  • 11.  The zygote develops a thick wall around itself and tides over the unfavourable period.  On the return of favourable conditions the diploid nucleus divides by meiosis into four haploid nuclei. Three of these nuclei degenerate.  On germination, wall of the zygote ruptures and a small tube like structure, containing one haploid nucleus comes out.  The small tube develops into 1 filament by repeated mitotic divisions.
  • 12.
  • 13. Reproduction in Angiosperms (Flowering plants)  Angiosperms reproduce both vegetative and sexual methods  Angiosperms reproduction occurs by fusion of male and female gametes present in the flower.  Angiosperms can be classified depending the time they take to complete life cycle and flowering  Annuals – Live for one year , produce flowers and seeds within one season  Biennials - two seasons, first year vegetative state, second year produce flowers, fruits and seeds  Perennials – plants which live for several years , one to few years vegetative stage then after that flowers, fruits and seeds every year  Monocarpic - plants are those that flower, set seeds and then die
  • 14. Plant Reproduction The Flower: The flower is the part of a plant that is involved in sexual reproduction
  • 15. Plant Reproduction  Initiation of flowering  The young plant grows vigorously and continue to grow till it attains a definite shape and size with its vegetative parts (roots, stem, leaves) well developed  Young or the Juvenile phase  Reproductive phase or adult phase  Vegetative shoot apex transforms into a reproductive or floral apex and starts bearing flowers
  • 16. Plant Reproduction The Parts of the Flower Sepals Petals Carpel Stamen
  • 17. Plant Reproduction Sepal: Green leaves which protect the flower when it is a bud Petals: Attract insects to the flower
  • 18. Plant Reproduction  The Stamen:  Male Part of the flower  Filament – stalk part of stamen  Anther – Small bag on top of the filament contains pollen
  • 19. Plant Reproduction  Structure of the Carpel:  Stigma – Pollen grains land here  Style – Connects stigma to the ovary  Ovary – Contains ovules  Ovules – Contain female gametes – eggs  Pistil - The ovule producing part of a flower
  • 20. Plant Reproduction Stages in Sexual Reproduction in Plants: Pollination Fertilisation and Seed Formation Seed Dispersal Germination
  • 21. Plant Reproduction Pollination: Happens when pollen from one flower is transferred to another flower of the same species Insect Pollination(Entomophily) or Wind Pollination (Anemophily)
  • 22. Plant Reproduction TWO TYPES OF POLLINATION Self pollination – transfer of pollen grains to stigma of same or another flower of same plant Cross pollination – transfer of pollen grains to stigma of a flower of another plant of the same species
  • 23. Importance of Pollination  It results in fertilization and ovule is converted into seed.  New varieties of plants are formed through new combination of genes in case of cross pollination.  During pollination pollen tube produces growth hormones which convert ovary into fruit.  Cross pollination is brought about by various external agencies such as, wind, insects, water, birds and other animals.
  • 24. Plant Reproduction  Insect Pollination:  Petals are bright and perfumed.  Insect rub against Anther and pollen gets stuck to them  Insect move to another plant of same species and pollen sticks to sticky stigma
  • 25. Plant Reproduction  Wind Pollination:  The flowers have long anthers which hang outside the flower.  Pollen is blown out of the anthers by the wind  The stigma of a wind-pollinated flower is very large and feathery – able to catch pollen
  • 26. Plant Reproduction  Fertilisation and Seed Formation:  After pollination, pollen tube develops out of the pollen grain and into the style,  It then enters the ovule and male gamete travels down to join with the egg – zygote  The zygote then becomes the seed.  The ovary will turn into the fruit
  • 27. Plant Reproduction Dispersal Of The Seed:  The major function of the fruit is to scatter the seed of the plant  Animal Dispersal  Wind Dispersal  Water Dispersal  Self Dispersal
  • 28. Plant Reproduction  Animal Dispersal:  Edible Fruits: Animal eats fruit and eventually passes the seed in its droppings, if seed falls in right place it will grow into new plant  Hooked Fruits: Fruits have tiny hooks on their surface, sticks to the animals.
  • 29. Plant Reproduction  Wind Dispersal:  The wind provides a very effective method of carrying fruit away from the parent plant  Dandelion uses parachute  Sycamore tree uses winged fruits
  • 30. Plant Reproduction  Water Dispersal:  The water lily is an example of a plant with fruits that can float.  Water currents carry the fruit away, with the seeds inside
  • 31. Plant Reproduction  Self Dispersal:  The gorse plant can scatter its own seed.  In summer the plant dries out and suddenly bursts.  The seeds shoot out from the fruit, and may land some distance from the parent plant
  • 32. Plant Reproduction  Germination:  Plumule – Will form shoot of plant  Radicle – Will become the root of the plant  Testa – Protects the seed  Food Store – Provides nourishment for the young plan
  • 33. Plant Reproduction  Conditions Necessary For Germination:  Water  Oxygen  Warmth
  • 34. Vegetative Reproduction in Angiosperms  Reproduction of new plants from the portion of the vegetative parts of a plant is very common and is called vegetative reproduction. Stems, roots, leaves and even buds are variously modified to suit this requirement. This is called natural vegetative reproduction.  Natural Method : In natural methods, a portion of the plant gets detached from the body of the mother plant and grows into an independent plant. The parts may be stem, root, leaf or even flower
  • 35.  The underground modification of stem, like rhizome, (in ginger), tuber (potato), bulb (onion) are provided with buds which develop into a new plant and therefore used to carry out vegetative propagation of the plant in the field. Plants with subaerial modification such as Pistia (offset) and Chrysanthemum (sucker) are also used for vegetative propagation.  Similarly, tuberous roots (Asparagus and sweet potato) can also be used for propagation as these roots have adventitious (form accidentally) buds which ground into a new plant.  Sometimes even leaves contribute to propagation of plants leaves of Bryophyllum that have buds on the margin and these buds grow into small plantlets. When detached from the mother plant they grow into independent plants.  In plants like Agave and Oxalis multicellular bodies called bulbils develop near the flower. These are called bulbils which when fall on the ground grow into new plant
  • 36. Vegetative Reproduction in Angiosperms  Humans have taken advantage of this natural phenomenon and have artificially propagated plants vegetatively by using the specialized parts as by cutting, grafting and layering.  Cuttings: Many plants like rose, Bougainvillea, Croton, Coleus, money plants, sugarcane etc. are grown through their stem cuttings. Cuttings of these plants can be grown even in water where they strike roots and develop adventitious buds  Layering: In this method, a lower branch of a plant is bent down and covered with moist soil leaving the growing tip above the soil. A ring of bark is removed from the stem before it is bent down. In a few weeks time when enough roots have developed on the underground portion above the ringed part, it. is cut off from the parent plant and grown separately as an independent plant.
  • 37. Vegetative Reproduction in Angiosperms  Aerial layering or Goatee : In this method a ring of bark is removed from a selected branch, and it is covered with moist moss and enclosed in a polythene sheet when roots appear, the stem is cut below the roots and planted to form a new plant
  • 38. Vegetative Reproduction in Angiosperms  Grafting – The purpose of grafting is to combine one plant's qualities of flowering or fruiting with the roots of another that offers vigour and resilience. This is a difficult task and requires lots of skill and practice. In most cases, trees and shrubs are available to buy already grafted onto a rootstock.  In this stock a branch is inserted which is known as scion or graft. This scion or graft is the stem cutting from the desired plant. Usually the grafted end of stock and scion fit well with each other and are bound firmly with tape or rubber-band until their tissues unite and vascular continuity is established. Grafting is mostly. practised in dicot plants
  • 39. Advantages and Disadvantages of Vegetative Reproduction  Advantages  (a) Rapid means of reproduction and spread.  (b) Offsprings identical to parent. The desired varieties can thus be preserved genetically for use.  (c) Food storage organs allow perennation or survival in adverse condition  (d) Improved varieties of ornamental plants and fruit trees can be multiplied easily.  (e) Vegetative propagation is a quicker, easier and a less expensive method of multiplying plants
  • 40.  Disadvantages  (a) Overcrowding and competition for space unless separated artificially.  (b) New varieties cannot be produced by this method except by mutation.  (c) Diseases typical of the species are rapidly transmitted and can decimate a crop

Editor's Notes

  1. Chlamydomonas loses flagella and becomes non-motile. Its protoplasm (cytoplasm and nucleus) divides mitotically and forms 4-8 zoospores. The parent cell wall is ruptured and zoospores are released. Each zoospore develops a cell wall and grows into an adult cell. The parent cell does not exist, any more The cell becomes non-motile by losing its flagella. The protoplasm divides mitotically into 2, 4, 8, 16, 32 daughter cells. Each daughter cell develops flagella and is released in water by the rupture of mother cell wall. It acts as a gamete. The gamete is morphologically identical in structure but all identical (Isogamous). Gametes released from two different mother cells fuse together. The contents of the gametes fuse and form a zygote (diploid). This is the only diploid stage in the life cycle of Chlamydomonas. The zygote develops a thick wall around itself to tide over unfavourable conditions (zygospores) On the return of favourable conditions (temperature, food and water) the diploid nucleus divides by meiosis and forms four haploid zoospores. Each zoospore grows into a new adult Chlamydomonas
  2. When, we use the vegetative parts for propagating crops or ornamental plants it is termed as artificial vegetative propagation. Cuttings : rose Layering : strawberry