Recommended
Recommended
More Related Content
What's hot
What's hot (20)
Similar to Tissue engineering Dr Hemali Goonasekera
Similar to Tissue engineering Dr Hemali Goonasekera (20)
More from costi2014
More from costi2014 (20)
Recently uploaded
Recently uploaded (20)
Tissue engineering Dr Hemali Goonasekera
- 1. National Consultative Workshop on Strengthening and Road mapping of Emerging Technology Innovation Systems of Sri Lanka Tissue Engineering Dr. Hemali Goonasekera MBBS(Colombo), D.Path., MD(Haematology) Senior Lecturer, Faculty of Medicine University of Colombo 17.11.2015
- 2. Tissue engineering (TE) Tissue Eng Part B Rev. 2015 Apr;21(2):203-17, IEEE Rev Biomed Eng. 2013;6:47-62. Main Aim Produce functional substitutes that can improve or replace a failing tissue Other Applications Cancer models, Drug models, Biorobotics Cells (Stem cells) Scaffolds Biologically active molecules Growth of new functional tissues / organs Biologically active molecules
- 3. Cell source - Mesenchymal stem cells (MSC) Ethical issues Tumour formation ? Efficacy of differentiation ? Introduction of somatic mutations Mesenchymal stem cells (MSCs) Adv: Multipotential differentiation & Immune modulatory effects Sources – Discarded tissue: Umbilical cord (UC), Adipose tissue
- 4. UC derived MSC– TE Applications (Registered on www.clinicaltrials.gov) Application – Diabetic Skin wounds MSC Delivery mechanisms – Direct (spraying, injecting) or systemic administration or via skin scaffolds Int. J. Mol. Sci. 2015; 16(10): 25476-501.
- 5. Scaffolds – Amniotic membrane (AM) AM for TE: anti- inflammatory, anti- scarring, & anti- angiogenic Dehydrated AM – original structure preserved Commercial products: EpiFix (dehydrated AM allograft) Acute and chronic wounds World J Stem Cells. 2015 Mar 26; 7(2): 428– 36.
- 6. TE Applications for SL Tissue Engineered (TE) product Application TE Skin replacement products Venous or pressure ulcers due to Diabetes / other critical ischaemia TE Bone and Cartilage Regeneration of damaged bone or cartilage TE Vascular grafts (TEVG) Coronary artery bypass grafting Peripheral artery bypass grafting TE Nerve Regeneration Spinal cord injury Drug models Nerve disease models Stem Cell source – UC & Adipose tissue The Tissue-Engineered Vascular Graft—Past,Present,and Future. Tissue Engineering Part B: Reviews. 2015. http://online.liebertpub.com/doi/pdfplus/10.1089/ten.teb.2015.0100 Perspectives on Tissue-Engineered Nerve Regeneration for the Treatment of Spinal Cord Injury Tissue Engineering Part A. 2014 Jul;20(13-14):1781-3. Endochondral Ossification for Enhancing Bone Regeneration:Converging Native Extracellular Matrix Biomaterials and Developmental Engineering In Vivo. Tissue Engineering Part B: Rev.2015 Jun;21(3):247-66.
- 7. Associated areas to Develop in SL Type Utility Mesenchymal stem cell (MSC) bank Availability of “off the shelf” MSC for TE and Research Amniotic membrane bank (Cryopreserved AM at -1960C) Use as a membrane graft – E.g. skin defects, ophthalmology Use to develop scaffolds – E.g. vascular grafts, skin grafts Development of Biobanks Biobanking: The Future of Cell Preservation Strategies. Adv Exp Med Biol.2015;864:37-53. Biobanking in a constantly developing medical world. Scientific World Journal. 2013 Sep 23;2013:343275.
- 8. Thank you
Editor's Notes
- Good afternoon ladies and gentleman. Thank you for inviting me to give a presentation in the area of Tissue Engineering.
- TE refers to the practice of combining cells, scaffolds, and biologically active molecules into functional tissues. And its aim is to restore, maintain, or improve damaged tissues or whole organs and additionally the developed tissue can be models to study development of disease in-vitro, become platforms for drug discovery and even be used in the field of biorobotics. Tissue Eng Part B Rev. 2015 Apr 1; 21(2): 203–217.
- One of the most important aspects of an engineered tissue construct is the cell source. Mesenchymal stem cells have been isolated from all most any tissue type of the body. Their ability for multipotent differentiation and immune modulatory effects, have made them good cell source for TE. Furthermore they can be easily obtained without great ethical conserns from UC or Adipose tissue, and these tissue derived stem cells has been used in TE applications which have gone upto clinical trail stage in clinical applications which are relavent in the Sri Lankan context. One example I have put here.
- These cells can be delivered to the wounds either directly (e.g., through spraying, injecting, or systemic administration) or via skin scaffolds
- One type of scaffold that has been used commercially is dehydrated amniotic membranes. AM a discarded product following partus can be easily obtained. which can Skin tissue engineering is the first field within tissue engineering that successfully engineered tissue in the laboratory. Skin tissue engineering utilizes biomaterials, stem cells, connective tissues and an established broad knowledge of the mechanism of the acute and chronic healing processes. EpiFix is a dehydrated amniotic membrane allograft. It is provided in sheets form, does not require FDA approval as human amniotic membrane is considered not significantly changed from its original structure. EpiFix is used in the treatment of acute and chronic wounds.
- Based on my limited experience in stem cell work, in our lab we have been able to isolate MScs from UC tissue and differentiae into bone, neural and endothelial tissue, the latter based on morphology only. Furthermore we have experience in cryopreserving AM. Therefore I would suggest that we can now think of moving forward to develop TE products according to GMP in the fields mentioned here. These mentioned areas of TE have been well studied and reviews are available in the literature.
- Furthermore I also think it is timely that in the same vein, we think of developing biobanks; as has been done in other countries; for storage of expanded MSCs obtained from UC/adipose tissue and AM for applications mentioned here as well as others. Thank you.