1. Attenuated Vaccines use: weakened microbes Killed microbes Microbe antigens all of the above 2. Which of the following is incorrectly matched? 1st line of defense - skins 2nd line of defense- Inflammation 3rd line of defense- immunoglobulins 3rd line of defense -complement 1st line of defense- Lysozyme 3. Which Leuckocyte is the most abundant in circulation? Macrophage Neurophil Plasma Cell Basophil.