paper presentation on the study the dose effective manner of particular plant species on the bone mineral density for the cure of Osteoporosis

1. PRESENTED BY
AYUSH JAIN
ROLL NO: 16
DEPARTMENT OF BIOTECNOLOGY, V.N.S.G.U
.

2. Introduction
 Osteoporosis: A condition in which a bone mineral density of 2.5 standard deviation or more
below the mean peak bone mass, measured by dual energy X-ray absoptiometry.
 Primary: Post-menopausal(Type-1) & Senile occurs after the age of 75 with ratio
2♀:1♂(Type-2)
 Secondary: Can arise in any age with equal sex ratio.

3. Background or Hypothesis
Investigation: In Vivo Observation and verification of the ameliorative effect of L.C on
Osteoporosis on mice 6 week old female
It has being subjected that L. chinense is most popular for counter effect on muscular Skeletal
diseases.
This concept was performed in ovarie-ectomized mice subjected with the extracts of the L.C
fruit using ethanol and B.M.C, B.M.D, concentration of Osteocalcin & Calcium was
determined to prove the hypothesis.

4. Results
Fig-1 L.C increased Low femoral B.M.C & B.M.D Levels
*p<0.05 compared with OVX .**p<0.01 compared with OVX. ***P<0.01 compared with SHAM, ****p<0.001 Compared with SHAM
SHAM-Normal Control OVX-Ovarie-ecctomzed E2-17-estradiol L.C1-L.C 1mg/Kg L.C100-L.C 100mg/Kg

5. Cont.
Fig-2- L.C recovered bone integrity. The growth plate thickness of the capital femoral epiphysis was shown by hematoxylin and
eosin staining (Magnification-100X)
E2-17-estradiol L.C-Lycium chinense extract OVX-Ovarie-ectomized

6. Cont.
Fig-3- L.C increased (A) serum osteocalcin & (B) Calcium Concentration
**p<0.05 compared with SHAM. ***p<0.001 compared with SHAM.
SHAM-Normal Control OVX-Ovarie-ecctomzed E2-17-estradiol L.C1-L.C 1mg/Kg L.C100-L.C 100mg/Kg

7. Discussion
 In the study ovariectomy decreased the femoral B.M.C & B.M.D, which is restored with oral
administration of L.C at 1mg/Kg & 100mg/Kg much higher then OVX set.
 Controlled remodeling between osteoblasts and osteoclasts mainly occurs to maintain bone
homeostasis. Moreover osteocalcin and calcium are classic markers of osteoblastic maturation.
 Why to use L.C
Alternative Approaches Disadvanatges
1. Hormone replacement
therapy(Estrogen)
Prolonged exposure associates with
breast cancer
2. Administration of Parathyroid
hormone
Prolonged treatment cause down-
regulation of bone forming genes

8. Conclusion
 The administration of L.C ameliorate osteoporosis in OVX. Moreover the shattering of bone
structure & replenishing of B.M.C and B.M.D were recovered by L.C along with Bone
markers.

9. References
 Song, L., Bi, Y. N., Zhang, P. Y., Yuan, X. M., Liu, Y., Zhang, Y., ... & Zhou, K. (2017).
Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region
Mice for Antiosteoporosis Research. BioMed research international, 2017.
 https://www.nhp.gov.in/disease/musculo-skeletal-bone-joints-/osteoporosis