2. FACTORS INFLUENCING GROWTH
• In the course of evolution, fungi are diverse field group as they survive in
a wide variety of habitats.
• Different species hence require different conditions for optimal growth.
• Microbial metabolism is significantly influenced by the physical and
chemical environment.
• Thus, toxin-producing fungi may invade food at pre-harvesting period,
harvest-time, during post-harvest handling and in storage.
3. TYPES OF FUNGI
• FIELD FUNGI
Includes species of plant pathogenic fungi, usually with
high requirements of water, such as Fusarium, Alternaria and
Cladosporium.
• ƒ
STORAGE FUNGI
With lower requirements of humidity, are principally the
genus Aspergillus and Penicillium.
• ƒ
ADVANCED DETERIORATION FUNGI
Normally do not invade intact food, but easily attack
damaged one and require high moisture content.
Some examples are some other Aspergilli species, Rhizopus,
Mucor and Absidia.
4. CONT…
• The main factors that influence growth and production of Aspergillus toxins
include temperature, pH and moisture.
• But apart from environmental factors, chemical and biological factors clearly
play a role (Figure 1).
• Under some circumstances these effects are additive.
• Under others, the implication is that synergistic interactions lead to a
combined effect of greater magnitude than the sum of constraints applied
individually.
6. ENVIRONMENTAL FACTORS
• The large and diverse group of microscopic foodborne yeasts and molds
includes several hundred species.
• These attack on many food, to maintained their population in the
environment and also to fulfil the environmental requirements.
• For this purposes, fungi use several major parameters which leading
fungal invasion, growth and production of mycotoxins.
7. TEMPERATURE
• Temperature is an important environmental factor affecting growth and
mycotoxin production by molds.
• Fungi are capable of surviving under the full range of temperatures
normally experienced in environments in which they live.
• The temperature range usually reported for fungal growth is broad (10-
28°C), with a few species capable of growth below or above this range.
• Fungi can be divided according to their tolerance to temperature in
psychrotolerant (Cryophiles) (−20°C to +10°C), mesophilic (20 to 45°C),
thermotolerant (high temperatures) and thermophilic fungi (50 and 55 °C).
8. HYDROGEN ION CONCENTRATION (pH)
• Hydrogen ion concentration in a medium could affect growth either
indirectly by its effect on the availability of nutrients or directly by
action on the cell surfaces.
• The acid/alkaline requirement for growth of all yeasts and molds is
quite broad, ranging from pH 3 to above pH 8, with optimum around
pH 5, if nutrient requirements are satisfied.
• In general, Aspergillus species are more tolerant to alkaline pH while
Penicillium species appear to be more tolerant to acidic pH.
9. AVAILABILITY OF OXYGEN
• Organisms can obtain energy by oxidative (respiratory) metabolism or by
fermentation.
• The implications for oxygen requirements of the occurrence of respiration,
fermentation or both in a fungus divided them in obligate aerobes, facultative
anaerobes and obligate anaerobes.
• Food spoilage molds, like almost all other filamentous fungi and yeasts, have an
complete requirement for oxygen.
• The most oxygen demanding molds will colonize the surface of the food, while
the less critical could be found inside the food.
10. CONT…
• However, black aspergilli have been reported as dominant genera
among most of the common mycoflora in grapes, such as;
• Alternaria,
• Cladosporium,
• Eurotium,
• Candida and some Penicillium .
11. WATER AVAILABILITY
• Living organisms consist largely of water.
• Hence if an organism is to grow has to take up water from the
environment.
• Whether water enters or leaves a cell depends on the difference
between the water potential of the cell and that of the surrounding
environment (medium).
• Water moving from a region of high to one of lower water potential is
osmosis.
• Fungi can easily grow in water moister environment.
12. COMPETING MICROFLORA
• The simultaneous presence of different microorganisms, as bacteria or
other fungi, could disturb fungal growth and the production of
mycotoxins.
• Therefore, several microorganisms have been reported as biological
pest control agents.
• It is seen that Trichoderma harzianum produces a lytic enzyme,
chitinase, which has an antifungal activity against a wide range of
fungal strains.
13. STRAIN VARIABILITY
• As already emphasized, the production of mycotoxins is influenced by
both the genotype of the organism and the physicochemical
environment in which it is growing.
• The production of any particular mycotoxin depends on the strain and
not only on the species.
14. INOCULUM
• The source of inoculum for most of the fungi affecting vegetal products
is likely the soil.
• They found significantly higher populations in summer months of the
year, and mycotoxigenic fungi were recovered more frequently from
crop residues in continuous culture than in culture rotation plots.
• It is known that the survival structure of most fungi in the soil may be
in debris or in soil, as mycelium or as conidia, and depending on the
fungi, as sclerotia.
15. NUTRITIONAL FACTORS
• Fungi can use a number of different carbon sources to fill their carbon
needs for the synthesis of carbohydrates, lipids, nucleic acids and
proteins.
• Oxidation of sugars, alcohols, proteins, lipids, and polysaccharides
provides them with a source of energy.
• Differences in their ability to utilize different carbon sources, such as
simple sugars, sugar acids, and sugar alcohols, are used, along with
morphology, to differentiate the various yeasts.
16. CONT…
• Fungi require a source of nitrogen for synthesis of amino acids for proteins, purines
and pyrimidines for nucleic acids, glucosamine for chitin, and various vitamins.
• Depending on the fungus, nitrogen may be obtained in the form of nitrate, nitrite,
ammonium or organic nitrogen as no fungus can fix nitrogen.
• Most fungi use nitrate, which is reduced first to nitrite and then to ammonia.
Therefore, availability and type of nutritional factors such as carbon source and
nitrogen source can also affect both mycotoxin production and morphological
differentiation.
• Other major nutrients for fungi are sulphur, phosphorus, magnesium and potassium,
which can be supplied to most fungi as salts.
• Trace elements like iron, copper, manganese, zinc and molybdenum are required by
nearly all fungi as cofactors for enzymes.
• But in high amounts, some trace elements can become toxic for some fungi.
Fusarium: large genus of filamentous fungi
Alternaria is a genus of Deuteromycetes fungi.
The main difference between yeast and mold is that yeast is a unicellular type of fungi whereas mold is multicellular filaments of the fungi. Mold consists of tubular branches with multicellular nuclei. The reproduction of yeast occurs by budding. Mold reproduces by the production of sexual or asexual spores.
Psychrophiles or cryophiles are extremophilic organisms that are capable of growth and reproduction in low temperatures, ranging from −20 °C to +10 °C. They are found in places that are permanently cold.
Water potential is the energy required, per quantity of water, to transport an infinitesimal quantity of water from the sample to a reference pool of pure free water.
DEFINITION. The water activity (a w) of a food is the ratio between the vapor pressure of the food itself, when in a completely undisturbed balance with the surrounding air media, and the vapor pressure of distilled water under identical conditions
relative humidity (RH) is a measure of the water vapor content of air.
Phage lytic enzymes are enzymes produced by bacterial viruses, either as part of their virion to facilitate bacterial infection through local peptidoglycan degradation, or as soluble proteins to induce massive cell lysis at the end of the lytic replication cycle.
Chitinases are hydrolytic enzymes that break down glycosidic bonds in chitin.
The genotype of an organism is its complete set of genetic material. Genotype can also be used to refer to the alleles or variants an individual carries in a particular gene or genetic location
strain is a genetic variant;