1. The proton motive force (PMF) generated by the electron transport chain is associated with which features? Options: A.It has a charge component such that it is more positive in the Mitochondrial inner membrane space than in the matrix B.The pH of the mitochondrial inner membrane space is higher than in the matrix C.Protons are pumped across the inner mitochondrial membrane by Complexes I-IV. D.It has a concentration component such that the [H+] is lower in the mitochondrial matrix than in the inner membrane space. E.The Phosphate transporter dissipates only the pH component of the PMF F.Exchange of ATP and ADP through a transporter dissipates both the pH and the charge component of the PMF. 2.Mitochondrial conditions determine the degree of reduction of each carrier in the respiratory chain. For example, when NADH and O2O2 are abundant, the steadystate degree of reduction of the carriers decreases as electrons pass from the substrate to O2.O2. When electron transfer is blocked, the carriers before the block become more reduced and those beyond the block become more oxidized. Match the state of oxidation for ubiquinone (Q) and cytochromes b, c , c, a, and a with each 1 3 mitochondrial condition. A.Abundant NADH and O2, but cynide added Match the following to the correct letter: 1. All carriers remain oxidized., 2. Early carriers undergo reduction, and later carriers B.ABUsndant NADH , but 02 exhausted. remain oxidized , 3. Early carriers remain oxideized , later carriers undergo reductio C.Abundant O2, but NADH exhausted 4.All carriers undergo reduction. D. ABUsndant NADH and O2 3.ATP-Synthase is powered by the PMF that is generated by the transfer of electrons from NADH or FADH2 to reduce oxygen. Which of the following are INCORRECT concerning ATP synthase and its mechanism? Options: A.The catalysis of the -subunits is allosterically regulated by the -subunits. B.The formation of mitochondrial cristae by ATP-synthase dimerization facilitates rapid exchange of substrates between the two enzymes. C.ATP synthase catalyzes a bimolecular reaction in which the transition state consists of a high energy conformation of the -subunit in the presence of ATP. D.The number of c-subunits in the F0 system varies across species resulting in differing ratios of ATP produced per Oxygen atom reduced. E.If the F0 and F1 were separated, F1 would promote hydrolysis of ATP.

1. 1. The proton motive force (PMF) generated by the electron transport chain is associated with
which features?
Options:
A.It has a charge component such that it is more positive in the Mitochondrial inner membrane
space than in the matrix
B.The pH of the mitochondrial inner membrane space is higher than in the matrix
C.Protons are pumped across the inner mitochondrial membrane by Complexes I-IV.
D.It has a concentration component such that the [H+] is lower in the mitochondrial matrix than in
the inner membrane space.
E.The Phosphate transporter dissipates only the pH component of the PMF
F.Exchange of ATP and ADP through a transporter dissipates both the pH and the charge
component of the PMF.
2.Mitochondrial conditions determine the degree of reduction of each carrier in the respiratory
chain. For example, when NADH and O2O2 are abundant, the steadystate degree of reduction of
the carriers decreases as electrons pass from the substrate to O2.O2. When electron transfer is
blocked, the carriers before the block become more reduced and those beyond the block become
more oxidized.
Match the state of oxidation for ubiquinone (Q) and cytochromes b, c
1
, c, a, and a
3
with each
mitochondrial condition.
A.Abundant NADH and O2, but cynide added Match the following to the correct letter: 1. All
carriers remain oxidized., 2. Early carriers undergo reduction, and later carriers
B.ABUsndant NADH , but 02 exhausted. remain oxidized , 3. Early carriers remain oxideized ,
later carriers undergo reductio
C.Abundant O2, but NADH exhausted 4.All carriers undergo reduction.
D. ABUsndant NADH and O2
3.ATP-Synthase is powered by the PMF that is generated by the transfer of electrons from NADH
or FADH2 to reduce oxygen. Which of the following are INCORRECT concerning ATP synthase
and its mechanism?
Options:
A.The catalysis of the -subunits is allosterically regulated by the -subunits.
B.The formation of mitochondrial cristae by ATP-synthase dimerization facilitates rapid exchange
of substrates between the two enzymes.
C.ATP synthase catalyzes a bimolecular reaction in which the transition state consists of a high
energy conformation of the -subunit in the presence of ATP.
D.The number of c-subunits in the F0 system varies across species resulting in differing ratios of
ATP produced per Oxygen atom reduced.
E.If the F0 and F1 were separated, F1 would promote hydrolysis of ATP